-
No products found
because this supplier's products are not listed.
Eamonn Reading, et al.,
bioRxiv - Biochemistry 2020
Quote:
... was then amplified from K-12 Escherichia coli chromosomal DNA (Zyagen Labs) and cloned into the pBR322 vector using In-Fusion® HD cloning (Takara Bio) ...
-
No products found
because this supplier's products are not listed.
Arthur J. Jallet, Arnaud Le Rouzic, Anne Genissel,
bioRxiv - Evolutionary Biology 2019
Quote:
... 50 mL of frozen cell suspension were lyophilized for 3 days (LYOVACTM GT 2-E freeze dryer, Steris) and ground in liquid nitrogen to a fine powder with a mortar and pestle ...
-
No products found
because this supplier's products are not listed.
Katherine Berman, et al.,
bioRxiv - Immunology 2024
Quote:
... A set of 87 COVID-19 negative serum samples collected between 3/24/2017 and 11/9/2018 was also purchased from Access Biologicals, LLC ...
-
No products found
because this supplier's products are not listed.
Karli A. Lipinski, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... 200 µL 425-600 micron acid-washed glass beads (Scientific Industries) and 300 µL 1:1 phenol/CHCl3 were added and cells were lysed by vortexing twice at high speed for 1 min with a 1 min rest on ice between ...
-
No products found
because this supplier's products are not listed.
Shuang Li, et al.,
bioRxiv - Microbiology 2021
Quote:
... live cells were stained with SYTO®9 and counted flow cytometrically with the CyFlow®Space (Sysmex Partec GmbH, Görlitz, Germany) by using the True Volumetric Absolute Counting mode and FloMax 2.4 (Sysmex Partec ...
-
No products found
because this supplier's products are not listed.
Gareth T. Banks, et al.,
bioRxiv - Neuroscience 2020
Quote:
... group housed mice were tagged with RFID micochips at 9 weeks of age and placed in the Home Cage Analysis system (Actual Analytics, Edinburgh) which captured mouse behaviour using both video tracking and location-tracking using RFID co-ordinates.
-
No products found
because this supplier's products are not listed.
Matthew Prideaux, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Antibody against fatty acid binding protein 4 (FABP4) was purchased from ProSci, Inc ...
-
No products found
Victor Danelon, et al.,
bioRxiv - Neuroscience 2022
Quote:
... following incubation in WesternBright HRP-substrate enhanced chemiluminescence (Advansta, catalog #K-12 045-D20) and exposure to Super RX-N X-Ray films (Fujifilm ...
-
No products found
because this supplier's products are not listed.
Franziska Blaeschke, et al.,
bioRxiv - Immunology 2022
Quote:
... cells were stained for 12 min with Dextramer-HLA-A*0201/SLLMWITQV-PE (Immudex) before adding surface antibodies ...
-
No products found
because this supplier's products are not listed.
C.J. Scavuzzo, L.A. Newman, P.E. Gold, D.L. Korol,
bioRxiv - Neuroscience 2020
Quote:
... Small burr holes were made in the skull overlying the hippocampus or striatum for guide cannulae (9 mm long, 350 µm diameter, BASi, West Lafayette, IN) and more laterally for hardware to attach the headcap ...
-
No products found
because this supplier's products are not listed.
Josua Oderbolz, et al.,
bioRxiv - Immunology 2021
Quote:
... M25 peptide (amino acid sequence: NHLYETPISATAMVI) was purchased from EMC microcollections (Tübingen, Germany) and 20 μg of the M25 peptide in 0.5% DMSO in 1x PBS solution were injected i.v ...
-
No products found
because this supplier's products are not listed.
NG Tolman, et al.,
bioRxiv - Genetics 2020
Quote:
... mice were acclimatized to the procedure room and anesthetized via an intraperitoneal injection of a mixture of ketamine (99 mg/kg; Ketlar, Parke-Davis, Paramus, NJ) and xylazine (9 mg/kg; Rompun, Phoenix Pharmaceutical, St. Joseph, MO) immediately prior to IOP assessment ...
-
No products found
because this supplier's products are not listed.
Angela Lai, et al.,
bioRxiv - Bioengineering 2024
Quote:
... Blood samples were collected at the inlet and outlet for measuring blood cell count (2 mL in K2EDTA) and aPTT/PT (3 mL in 1:9 citrate) using a clinical hematology analyzer (Diagnostica Stago Start 4, Siemens, Germany).12 If aPTT was outside the range of 20-50 seconds ...
-
No products found
because this supplier's products are not listed.
Colin Kremitzki, et al.,
bioRxiv - Genetics 2023
Quote:
... contents from the nucleofection were transferred to 12-well plates (TPP 92012, MIDSCI, Fenton, MO, USA) with 2mL of McCoy’s media and were allowed to proliferate for 7 days ...
-
No products found
because this supplier's products are not listed.
Xingyu Lu, et al.,
bioRxiv - Biochemistry 2021
Quote:
... and cholesterol were purchased from Sigma-Aldrich Chemical Company (St. Louis, MO, USA) and arachidonic acid was purchased from Oxford Biomedical Research, Inc (Oxford Biomedical Research ...
-
No products found
because this supplier's products are not listed.
Hannah J. Larsen, et al.,
bioRxiv - Physiology 2022
Quote:
... Arachidonic Acid (P/N 390) and ADP (P/N 384) were purchased from Chrono-Log Corporation ...
-
No products found
because this supplier's products are not listed.
Laura M. Chambers, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... cells were plated at 65 % confluence in a 100 mm dish and cultured in 9 mL DMEM supplemented with heat inactivated 10% FBS (Atlas Biologicals Cat # F-0500-D, Lot F31E18D1)[15] ...
-
No products found
because this supplier's products are not listed.
Harwin Sidik, Christy J. Ang, Mahmoud A. Pouladi,
bioRxiv - Developmental Biology 2019
Quote:
... 10-12 embryos per genotype were pooled for RNA extraction using the Total Tissue RNA Mini Kit (Favorgen) and total RNA is eluted in 15 µl volumes ...
-
No products found
because this supplier's products are not listed.
Rishel B. Vohnoutka, et al.,
bioRxiv - Biochemistry 2021
Quote:
Periodic Acid Schiff Staining with and Without Diastase: All reagents were obtained commercially from Rowley Biochemical Inc (Danvers ...
-
No products found
because this supplier's products are not listed.
Sandra L. Bixler, et al.,
bioRxiv - Microbiology 2021
Quote:
Total SARS-CoV-2 E gene and subgenomic E gene target copy numbers were determined by real-time RT-PCR using previously described assays (24–25) and a synthetic RNA containing the subgenomic E RNA sequence (Bio-Synthesis, Inc., Lewisville, TX, USA). Extracted nucleic acid was tested in triplicate (5 μL extracted nucleic acid ...
-
No products found
because this supplier's products are not listed.
Wilfred A. Jefferies, et al.,
bioRxiv - Immunology 2023
Quote:
... or LNP-B.1.617.2 (Delta) spike protein variant of the SARS-CoV-2 virus (Cat # PM-LNP-12) mRNA purchased from Promab Biotechnologies ...
-
No products found
because this supplier's products are not listed.
Kilea Ward, Hongmei Li-Byarlay,
bioRxiv - Physiology 2021
Quote:
... The assay was conducted using the OXItek™ Thiobarbituric Acid Reactive Substances (TBARS) Assay Kit (ZeptoMetrix Corp). The head of each bee was flash-frozen in liquid nitrogen and immediately pulverized in a 1.5 mL microcentrifuge tube with a sterile plastic pestle ...
-
No products found
because this supplier's products are not listed.
Annie R. Bice, et al.,
bioRxiv - Neuroscience 2021
Quote:
Transcutaneous electrical stimulation was applied to the left and right forepaws as previously described [12] by placing microvacular clips (Roboz) on either side of the wrists ...
-
No products found
because this supplier's products are not listed.
Maurizio Chioccioli, et al.,
bioRxiv - Cell Biology 2022
Quote:
... PCLSs were kept immobilized and flat on the glass bottom by placing a 12 mm diameter porous membrane (0.4 μm) inlet-opening system (BrandTech 782821) on top of the PCLS and weighing it down with 1.66 g flat metal washer (Fig 1b) ...
-
No products found
because this supplier's products are not listed.
Elizabeth A. Kiffmeyer, et al.,
bioRxiv - Neuroscience 2022
Quote:
All mice were housed on a 12-hour light-dark cycle (7 a.m. - 7 p.m.) in open top mouse cages (Ancare, Bellmore, NY) in groups of 2-5 littermates per cage ...
-
No products found
because this supplier's products are not listed.
Alexandra L. Obukhova, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... and then incubated for 12–24 h at 4 °C with rabbit antibodies against 5-HT (rabbit, Immunostar, Cat #20080) diluted 1:1000 in 0.5% PBS-TX ...
-
No products found
because this supplier's products are not listed.
Kyle B. Williams, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... drug was added in triplicate or quadruplicate wells per dose in a 8- point or 12-point dose-response manner (depending on experiment, as indicated) using the acoustic Echo 550 liquid dispenser (Labcyte). Drug combination testing was done in a similar manner and plated in a constant ratio as indicated ...
-
No products found
because this supplier's products are not listed.
Hongji Yan, et al.,
bioRxiv - Bioengineering 2019
Quote:
The sialic acid removal assay was conducted by using Neuraminidase immobilized on slurry (GlycoCleave Neuraminidase kit, GALAB technologies). Briefly ...
-
No products found
because this supplier's products are not listed.
Zhe Li, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... 2 μL 1 M imidazole and 88 μL of 5 nm gold nanoparticles (AuNPs) functionalized with nickel (II) nitriloacetic acid (NTA) chelates (0.5 μM in 50 mM MOPS, pH 7.9, Nanoprobes) were mixed for nanoparticle encapsulation ...
-
No products found
because this supplier's products are not listed.
Victor Hugo Souza, et al.,
bioRxiv - Neuroscience 2021
Quote:
... was recorded from the right abductor pollicis brevis (APB) using surface electrodes in a belly—tendon montage (Neuroline 720, Model 72001-K/12; Ambu, Denmark) with a Nexstim eXimia EMG device (500-Hz lowpass filtering ...
-
No products found
because this supplier's products are not listed.
Pedro J. Llanos, et al.,
bioRxiv - Cell Biology 2021
Quote:
... cells were washed twice and supplemented either with no cytokines or with human (h) IL-2 (200 ng/ml) and mouse (m) mIL-12 (10 ng/ml) from Shenandoah Biotechnology alone or in combination every three days.
-
No products found
because this supplier's products are not listed.
Chuandong Wang, et al.,
bioRxiv - Microbiology 2023
Quote:
... Pipet 2.5 mL or 150 μL of adjusted bacterial cultures into each well of 12- or 96-well microplates (NEST Biotechnology, China), respectively ...
-
No products found
because this supplier's products are not listed.
Yasuhiro Takenaka, et al.,
bioRxiv - Cell Biology 2021
Quote:
Production of hydroxyl radical (.OH) and hypochlorous acid (HClO) were measured using the OxiORANGE reagent (Goryo Chemical, Sapporo, Japan). H2O2 and NO were detected by HYDROP and diaminofluorescein-FM diacetate (DAF-FM DA)(Goryo Chemical ...
-
No products found
because this supplier's products are not listed.
Sandra Schwarz, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... respective wells were treated with 400µM PI-9i (1,3-Benzoxazole-6-carboxylic acid, Advanced ChemBlocks Inc, Hayward, CA, USA). Following pre-treatment ...
-
No products found
because this supplier's products are not listed.
Narayan Pokhrel, et al.,
bioRxiv - Evolutionary Biology 2022
Quote:
... were collected from a commercial farm and stored at 18°C or 12°C up to 28 d in a cooled incubator (SN 265959, VELP SCIENTIFICA, Italy). The temperature and relative humidity of cooled incubator was continuously monitored throughout the experiment ...
-
No products found
because this supplier's products are not listed.
Raeann Goering, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Denatured lysates were separated by PAGE on 4%–12% Bis-Tris gradient gels along with Flash Protein Ladder (Gel Company FPL-008) using MOPS SDS NuPAGE Running Buffer (Thermo Fisher ...
-
No products found
because this supplier's products are not listed.
Julie Zimmermann, et al.,
bioRxiv - Immunology 2021
Quote:
... and monomycoloyl glycerol (17) were obtained from NCK A/S (Farum, Denmark) and polyinosinic:polycytidylic acid was bought from Dalton Pharma Services (North York ...
-
No products found
because this supplier's products are not listed.
John J. McInnis, et al.,
bioRxiv - Neuroscience 2023
Quote:
... in 47.5 mL borate buffer (100 mM Boric Acid, 75 mM Sodium Chloride, pH 8.4, Boston Bioproducts Inc. C-8852S) overnight at room temperature ...
-
No products found
because this supplier's products are not listed.
Oleg A. Sineshchekov, et al.,
bioRxiv - Biochemistry 2019
Quote:
... was protected from excitation laser flashes by a second monochromator of the same type and additionally with 12-nm bandwidth interference filters (Oriel Instruments, Stratford, CT). Signals were amplified by a low noise current amplifier (model SR445A ...
-
No products found
because this supplier's products are not listed.
Saeed Roschdi, et al.,
bioRxiv - Molecular Biology 2021
Quote:
(GU)11G and (GU)12 were screened for crystallization using 200 nL RNA solution and 200 nL reservoir solution using a Mosquito (TTP Labtech, Cambridge, MA.) RNA solution consisting of 0.72 mM RNA ...
-
No products found
because this supplier's products are not listed.
Mario Figueroa, et al.,
bioRxiv - Physiology 2023
Quote:
... C57Bl/6 mice (12-20 weeks of age) underwent subcutaneous left flank implantation of loaded Alzet osmotic mini pump (model 1004; Durect Corporation, Cupertino, CA). C57Bl/6 mice received either an AngII infusion (C57Bl/6+AngII) ...
-
No products found
because this supplier's products are not listed.
Shouka Parvin Nejad, et al.,
bioRxiv - Bioengineering 2023
Quote:
... The insoluble elastin content of oxalic acid digested samples was measured using the FastinTM Elastin Assay Kit by Biocolor (Accurate Chemical and Scientific Corporation ...
-
No products found
because this supplier's products are not listed.
Ava Niazi, et al.,
bioRxiv - Developmental Biology 2024
Quote:
Antibody to TECTA was raised to peptides based on the predicted amino acid sequence of the mouse TECTA protein (CYNKNPLDDFLRPDGR) and was purified by antigen specific affinity purification by Abfrontier (www.gwvitek.com). The purified antibody (1 mg/ml ...
-
No products found
Guneet Kaur, et al.,
bioRxiv - Neuroscience 2023
Quote:
Primary human cerebral cortex microvascular endothelial cells (Passage 3, 12 CPD in vitro, ACBRI 376) were procured from Cell Systems (Kirkland, WA 98034, USA). Brain microvascular endothelial cells (BMECs ...
-
No products found
because this supplier's products are not listed.
de la O Sean, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... + 0.22 g glucose (MilliporeSigma, G7528) + 1.23 g sodium bicarbonate (MilliporeSigma, S5761) + 10 g fatty-acid free bovine serum albumin (FAF-BSA) (Lampire Biological Laboratories, 7500812) + 10 μL Insulin-Transferrin-Selenium-Ethanolamine (ITS-X ...
-
No products found
because this supplier's products are not listed.
Andrea D. Raymond, et al.,
bioRxiv - Microbiology 2019
Quote:
Nef concentration in acid-dissociated microvesicle/exosome preparations was measured using a commercially available anti-Nef sandwich ELISA kit (Immunodiagnostics, Bedford, MA) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Bassem D. Khalil, et al.,
bioRxiv - Cancer Biology 2021
Quote:
HEK293T or D-HEp3 cells were transfected with cDNA for reporter vectors where firefly luciferase expression is driven by retinoic acid response element (RARE) [30] or NR2F1 cis element (Signosis #LR-2029). Cells were co-transfected with Renilla luciferase as an internal control ...
-
No products found
because this supplier's products are not listed.
Jasmin A. Schäfer, et al.,
bioRxiv - Cell Biology 2019
Quote:
... folic acid and riboflavin (Formedium, Norfolk, UK) and 0.2 µg/ml rapamycin in 96-well glass bottom plates (Brooks Life Sciences, Chelmsford, Massachusetts) coated with concanavalin A ...
-
No products found
because this supplier's products are not listed.
Michael F. Coleman, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... control and PC-suppressed E0771 tumors grew for 12 days and then were randomized to begin daily treatment with AZD3965 by oral gavage (50mg/kg) (MedKoo Biosciences, Morrisville, NC, #1448671-31-5). After 14 days of treatment ...
-
No products found
because this supplier's products are not listed.
Manoj Arra, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Supernatant was used immediately for lactic acid assay to measure secreted lactate in the media using a 1:20 dilution (Cat# 1200011002, Eton Biosciences, San Diego, CA USA). Unconditioned DMEM with 10% FBS was used as a control for subtracting background.