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No products found
because this supplier's products are not listed.
Icaro Putinhon Caruso, et al.,
bioRxiv - Biophysics 2021
Quote:
... Samples of 20 μΜ N-NTD or N-NTD-SR consisting of varied protein:nucleic acid molar ratios (8:1, 4:1, 2:1, 1:1, 1:2) were prepared in low-binding microtubes (Eppendorf® LoBind) in the presence of 10% PEG-4000 (w/v ...
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No products found
because this supplier's products are not listed.
A.R. von Gundlach, et al.,
bioRxiv - Biophysics 2019
Quote:
... after in situ activation with four equivalents of N,N,N′,N′-Tetramethyl-O-(1H-benzotriazol-1-yl)uronium hexafluorophosphate (HBTU; Carbosynth, Berkshire, United Kingdom) and eight equivalents of N-Methylmorpholine (NMM ...
-
No products found
because this supplier's products are not listed.
Daniel F. Comiskey Jr., et al.,
bioRxiv - Cancer Biology 2019
Quote:
2’O-methyl SSOs were provided by Trilink BioTechnologies ...
-
No products found
because this supplier's products are not listed.
Súil Collins, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... The devices were silanized by pipetting a fresh solution of 2% Trichloro(1H,1H,2H,2H-perfluorooctyl)silane in HFE-7500 (3M) into the chips ...
-
No products found
because this supplier's products are not listed.
Sara Lago, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... Triton 0.3% in PBS) for 1h RT and incubated overnight with primary antibodies against pRPA32 1:1000 (Bethyl, A300-246A-8), anti-human-mitochondria (Abcam ...
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No products found
because this supplier's products are not listed.
Annika Ullrich, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...
-
No products found
because this supplier's products are not listed.
Jordan J. Aoyama, Medha Raina, Gisela Storz,
bioRxiv - Microbiology 2021
Quote:
... was separated on denaturing 8% polyacrylamide gels containing 6 M urea (1:4 mix of Ureagel Complete to Ureagel-8 (National Diagnostics) with 0.08% ammonium persulfate ...
-
No products found
because this supplier's products are not listed.
Xuyong Chen, Lingling Liu, Ruoning Wang,
bioRxiv - Immunology 2022
Quote:
... anti–mIL-4 (8 μg/mL, Bio X Cell), and anti–mIFN-γ (8 μg/mL ...
-
No products found
because this supplier's products are not listed.
Florence E. McLean, et al.,
bioRxiv - Microbiology 2024
Quote:
... 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) 25 mM (Lonza, 17-737F), gentamicin 25 μg/ml (Lonza ...
-
No products found
because this supplier's products are not listed.
Els F. Halff, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and incubated for 1h at RT with 1:1000 mouse anti-MAP2 (Synaptic Systems, 188-011), followed by 45-60 min incubation with the appropriate Alexa-fluorophore conjugated secondary antibodies (goat-anti-mouse Alexa405 ...
-
No products found
because this supplier's products are not listed.
Lucie Lanikova, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... or Stellaris 8 Falcon with 63×/1.3 objective (4 × zoom; Leica Microsystems, Wetzlar, Germany). Nuclei were identified based on 4′,6-diamidino-2-phenylindole (DAPI ...
-
No products found
because this supplier's products are not listed.
Yisrael Rappaport, et al.,
bioRxiv - Cell Biology 2020
Quote:
... rabbit anti-HIM-8 (Novus Biological, 1:2000), rat anti-HIM-8 (1:100 ...
-
No products found
because this supplier's products are not listed.
Naba Al-Sari, et al.,
bioRxiv - Biophysics 2020
Quote:
... trioctanoin-1,1,1-13C3 (TG(8:0/8:0/8:0)-13C3) and 1-palmitoyl-2-hydroxy-sn-Glycero-3phosphatidylcholine (LPC(16:0)) from Larodan AB (Solna ...
-
No products found
because this supplier's products are not listed.
Li Zhenyu, Li Tian, Liu Meisui, Ivanovic Tijana,
bioRxiv - Microbiology 2022
Quote:
... sn-(1-oleoyl-2-hydroxy)-glycerol-3-phospho-sn-3′-(1′-oleoyl-2’-hydroxy)-glycerol (ammonium salt) (18:1 BMP (R,R); Avanti Polar Lipids) ...
-
No products found
because this supplier's products are not listed.
Aude Noiret, Fabienne Aujard, Jeremy Terrien,
bioRxiv - Evolutionary Biology 2023
Quote:
... ref MS E-5100) and 8-hydroxy-2’-deoxyguanosine (“8-OHdG,” in ng.ml− 1; OxiSelectTM Oxidative DNA Damage Elisa kit, Cell Biolabs Inc., ref STA-320) were measured in duplicates from urine samples as indicators of the organisms’ response to environmental stress (Miller et al. ...
-
No products found
because this supplier's products are not listed.
Logan D. Morton, et al.,
bioRxiv - Bioengineering 2022
Quote:
... and O-(1H-6-Chlorobenzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HCTU) coupling reagent (99.9%, Chem-Impex International, Inc.), and a ten-fold excess of N-methylmorpholine (99% ...
-
No products found
because this supplier's products are not listed.
Md Gulam Musawwir Khan, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Cell survival was measured using the WST-8 (water-soluble Tetrazolium-8: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)- 5- (2,4-disulfophenyl)-2H-tetrazolium) assay kit (CCK-8; Dojindo Molecular Technologies, #CK04) following manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Bianca Dietrich, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... inhibition of NOTCH signalling in P1 organoids was achieved by adding either 50 µM [(2R,4R,5S)-2-benzyl-5-(Boc-amino)-4-hydroxy-6-phenyl-hexanoyl]-Leu-Phe-NH2 trifluoroacetate salt (L-685,458; Bachem) or 10 µM Deshydroxy LY-411575 (DBZ ...
-
No products found
because this supplier's products are not listed.
Filip Bergqvist, et al.,
bioRxiv - Immunology 2019
Quote:
IL-8 was quantified in plasma by Human IL-8 (CXCL8) ELISA development kit (3560-1H, Mabtech) according to manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Brajabandhu Pradhan, et al.,
bioRxiv - Microbiology 2020
Quote:
... and bead beating 4 times for 2 minutes in a Mini-BeadBeater-8 (BioSpec) with cooling on ice in between.
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No products found
because this supplier's products are not listed.
Whitney N. Costello, et al.,
bioRxiv - Biophysics 2023
Quote:
... Uniformly-labeled 13C 15N Sup35NM samples were prepared by growing BL21(DES)-Rosetta Escherichia coli in the presence of M9 media with 2 g L-1 D-glucose 1H,13C6 and 1 g L-1 of 15N ammonium chloride (Cambridge Isotope Labs, Cambridge, MA). Purified ...
-
No products found
because this supplier's products are not listed.
Shaibu Oricha Bello, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... Neutral red (3-amino-7-dimethylamino-2-methyl-phenazine hydrochloride) (Solarbio, cat. N8160), Minimal Essential Medium/Earls Balance Salts (MEM/EBSS ...
-
No products found
because this supplier's products are not listed.
Miao-Hsi Hsieh, et al.,
bioRxiv - Microbiology 2020
Quote:
... coverslips were blocked with 2% w/v BSA for 1h and incubated with ACE2 antibody [SN0754] (1:250) (GeneTex, GTX01160), followed by Goat anti-rabbit IgG (H+L ...
-
No products found
because this supplier's products are not listed.
Guojia Xie, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... and incubated with 4-8 μg primary target antibody and 2 μg spike-in antibody (Active Motif, #61686) overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Alexia Caillier, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The PVDF membranes were incubated overnight at 4◦C in 2% BSA (Research Products International, 9048-46-8) with primary antibody ...
-
No products found
because this supplier's products are not listed.
Eric Batsché, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... treated by RNase A (20 µg/mL) for 1h at 37°C in TE buffer and sonicated 8 min with BioRuptor (Diagenode) (15 sec ON / 15 sec OFF ...
-
No products found
because this supplier's products are not listed.
Alexandria N. Miller, et al.,
bioRxiv - Biophysics 2022
Quote:
... 1 mM 4-(2-Aminoethyl)-benzenesulfonylfluoride hydrochloride (AEBSF, Gold Biotechnology), 1 mM benzamidine hydrochloride monohydrate (benzamidine ...
-
No products found
because this supplier's products are not listed.
Domenico Iuso, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... The resulting bottom organic phase was dried and on-line derivatized to fatty acid methyl ester by a chloroform/methanol (1:2)-TMSH solution (Macherey-Nagel) and analyzed by gas chromatography-mass spectrometry (Agilent 5977A-7890B) ...
-
No products found
because this supplier's products are not listed.
Yang Zhang, et al.,
bioRxiv - Physiology 2021
Quote:
... and Di-8-ANEPPS (Biotium, #61012, 2 µM) or Wheat Germ Agglutinin Alexa Fluor™-488 Conjugate (Invitrogen™ ...
-
No products found
because this supplier's products are not listed.
Kazuki Moroishi, et al.,
bioRxiv - Bioengineering 2022
Quote:
... 1H NMR (JEOL, Tokyo, Japan) (400 MHz ...
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No products found
because this supplier's products are not listed.
Sebastian Duno-Miranda, et al.,
bioRxiv - Molecular Biology 2023
Quote:
The fluorescence of 3’-O-(N-Methyl-anthraniloyl)-2’-deoxyadenosine-5’-triphosphate (mantATP) (Jena Biosciences) was measured with 290 nm excitation and a 395 nm long-pass emission filter in a stopped-flow apparatus (Applied Photophysics ...
-
No products found
because this supplier's products are not listed.
Ulrich Hohmann, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... and incubated for 1h at 4 °C before being added to 10 µl magnetic V5 beads (v5tma, Chromotek), pre-equilibrated in buffer K ...
-
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...
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Chunlei Cang, Boxun Lu, Dejian Ren,
bioRxiv - Physiology 2020
Quote:
... 4 mg collagenase type 2 (Worthington) and 1.5 mg trypsin (Worthington) ...
-
No products found
because this supplier's products are not listed.
Jihae Shin, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... The 5’ and 3’ end 2’-O-Methyl and phosphorothioate modified sgRNAs were synthesized by Genscript (Piscataway, NJ).
-
No products found
because this supplier's products are not listed.
Irina V. Chestakova, et al.,
bioRxiv - Microbiology 2023
Quote:
... A small fraction of about 4×4 mm from each tissue was transferred to a 2 mL vial with a 1/4” ceramic sphere (MP Biomedicals, Solon OH, USA) and 300 μL MagNA Pure 96 DNA Tissue Lysis Buffer (Roche Diagnostics GmbH ...
-
No products found
because this supplier's products are not listed.
Yixin Xu, et al.,
bioRxiv - Biophysics 2023
Quote:
... 0.5 μL L-PEG-L (8 mg/ml) and 0.5 μL Pd(NO3)2 (1 mg/ml) was applied to holey carbon grid (Quantifoil 200 mesh Cu R2/2) which was glow discharged 45 s at 15 mA ...
-
No products found
because this supplier's products are not listed.
Alexia Polissidis, et al.,
bioRxiv - Neuroscience 2021
Quote:
For GBA quantification (n=5 for 4 WPI, n=3 for 8 WPI), the surface function of the Imaris software was used to select only GFP-positive cells by appropriately adjusting the number of voxels as well as the intensity of the GFP channel in the threshold tab ...
-
No products found
because this supplier's products are not listed.
Ancély F. dos Santos, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Fluorescence intensity values were collected every 2 min during 1h at 500 nm in a SpectraMax M2 microplate reader (Molecular Devices). Activity units were calculated as ...
-
No products found
because this supplier's products are not listed.
Hiroyuki Okada, et al.,
bioRxiv - Genomics 2024
Quote:
Pico Methyl-Seq Library Prep Kit (Zymo research) was used for making WGBS libraries basically as manufacturer’s instruction ...
-
No products found
because this supplier's products are not listed.
Nicole Y. Lai, et al.,
bioRxiv - Immunology 2019
Quote:
... NKM-PE (16-2-4) from Miltenyi Biotec, Vγ7-FITC (kindly provided by P ...
-
No products found
because this supplier's products are not listed.
Wouter H. van Megen, et al.,
bioRxiv - Physiology 2021
Quote:
8 WT and 8 Nuf/+ mice (age 16-17 weeks) were placed on a 2%-supplemented Ca2+ diet (Envigo, Indianapolis, IN, USA) for 7 days ...
-
No products found
because this supplier's products are not listed.
Anjali Sengar, et al.,
bioRxiv - Microbiology 2023
Quote:
... The membrane was blocked with 5% BSA in TBS containing 0.1% tween-20 (TBS-T) for 1h at 4 °C before incubating with anti-S2 Spike primary antibody (rabbit polyclonal, Sino Biologicals, Cat#: 40590-T62) at 1:1,000 dilution in TBS-T with 5% BSA for 14-16 h at 4°C ...
-
No products found
because this supplier's products are not listed.
Alice M. H. Bedois, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... embryos were cleared for 1h in Optiprep (Stem Cell Technology). For dorsal imaging of the developing head ...
-
No products found
because this supplier's products are not listed.
Mukund Madhav, et al.,
bioRxiv - Microbiology 2019
Quote:
... were subcultured at a ratio of 1:2 every 2 - 4 weeks and maintained in 25 cm2 culture flasks (Cellstar; Greiner Bio-One, Monroe NC) at 32°C ...
-
No products found
because this supplier's products are not listed.
Ruchi Malik, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Patch electrodes (2–4 MΩ) were pulled from borosilicate capillary glass of external diameter 1 mm (Sutter Instruments) using a Flaming/Brown micropipette puller (model P-2000 ...
-
No products found
because this supplier's products are not listed.
Jeffrey L. Platt, et al.,
bioRxiv - Immunology 2021
Quote:
To detect mouse Ig Nunc MaxiSorp ELISA plates were coated for 1h at room temperature with goat anti-mouse Ig (H+L) (4 μg/ml; Southern Biotech, Cat#1010-01; RRID: AB_2794121). Citrobacter rodentium-specific Ig was quantified by coating Nunc MaxiSorp ELISA plates with 108 bacteria/well heat-inactivated at 60°C for 1 h ...
-
No products found
because this supplier's products are not listed.
Aaron T. Crain, et al.,
bioRxiv - Genomics 2024
Quote:
... Membranes were blocked in 5% milk in TBS- Tween for 1h prior to incubation with α-GFP (1:1000, Rockland) or α-Set8 (1:1000 ...
-
No products found
because this supplier's products are not listed.
Abdelrahim Zoued, et al.,
bioRxiv - Microbiology 2021
Quote:
... for 1h at RT without agitation and observed by light microscopy (Nikon Ti Eclipse equipped with a metal-oxide-semiconductor (sCMOS ...
-
No products found
because this supplier's products are not listed.
Trevor S. Wendt, Rayna J. Gonzales,
bioRxiv - Physiology 2023
Quote:
... and for 1h at RT goat anti-mouse IRDye 680LT (LI-COR) which served as a loading control ...
-
No products found
because this supplier's products are not listed.
Kirsten J. Meyer, Justin R. Nodwell,
bioRxiv - Microbiology 2023
Quote:
... supernatant or size exclusion purified vesicles were extracted with butanol (1:2) overnight at 4°C and the butanol fraction transferred to microtiter plates for absorbance measurement (Synergy, Biotek).