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The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell...
Cat# B34304, SKU# B34304-25 mL (2500 rxns),
25 mL (2500 rxns), $201.00
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Kristina A.M. Arendt, et al.,
bioRxiv - Cancer Biology 2020
Quote:
In vitro cell proliferation was determined using WST-8 [water soluble tetrazolium-8 or 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulphophenyl)-2H-teterazolium] assay (Bimake; Munich, Germany). For this ...
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No products found
because this supplier's products are not listed.
Nicholas Dillon, et al.,
bioRxiv - Microbiology 2020
Quote:
... hexakis (1H,1H,2H-difluoroethoxy)-phosphazene (SynQuest Labs, Inc.) was used as a “lock mass” internal calibrant (m/z 622.028960 ...
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No products found
because this supplier's products are not listed.
Aaron T. Crain, et al.,
bioRxiv - Genomics 2024
Quote:
... Membranes were blocked in 5% milk in TBS- Tween for 1h prior to incubation with α-GFP (1:1000, Rockland) or α-Set8 (1:1000 ...
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No products found
because this supplier's products are not listed.
Yiran Li, et al.,
bioRxiv - Genomics 2020
Quote:
... 2 □1 water and 5□1 MyTaq™ HS Mix (Bioline). RT-PCR was performed in the following conditions ...
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No products found
because this supplier's products are not listed.
Kathryn P. Trogden, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 5-8 islets per 10 mm glass bottomed dish (Mattek) were placed in the center of the glass in 100 ul RPMI 1640 and allowed to settle for 1.5-2 hours before being transferred to 5% CO2 at 37°C ...
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No products found
because this supplier's products are not listed.
Alexandros Sfikas, et al.,
bioRxiv - Cell Biology 2019
Quote:
... siRNA 2 5’ GCCCUAUCCCUUUACGUCA (Eurogentec). Dharmacon ONTarget plus SMARTpool ...
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No products found
because this supplier's products are not listed.
Riffat Mehboob, et al.,
bioRxiv - Pathology 2020
Quote:
... NK-!R antibody (Abbott, 1:1000) was applied and incubated for 30 min at 37 °C ...
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No products found
because this supplier's products are not listed.
Jane S. Huang, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and GCaMP6s (GFP-Booster-Atto-488 [1:500, 1h at room temperature, gba-488-100, Chromotek]). Confocal z-stacks of ∼1 mm of dorsal septal OE were collected (voxel size ...
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No products found
because this supplier's products are not listed.
Yohan S.S. Auguste, et al.,
bioRxiv - Neuroscience 2022
Quote:
... subcutaneous] before being anesthetized using isoflurane (SomnoSuite, Kent Scientific; 3-5% induction, 1-2% maintenance). Once anesthetic depth was achieved ...
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No products found
because this supplier's products are not listed.
Yahor Savich, et al.,
bioRxiv - Biophysics 2019
Quote:
... and RLC was incubated at 4°C for 1hr in 5-fold molar excess of bifunctional 3,4-Bis-(methanethiosulfonylmethyl)-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-1-yloxy spin label (BSL; Toronto Research Chemicals). The covalent disulfide double bond between BSL and the 𝛼-helix backbone produces bifunctional stereospecific attachment in this labeling conditions (Fig ...
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No products found
because this supplier's products are not listed.
Laurell Kessler, et al.,
bioRxiv - Biophysics 2024
Quote:
... U-2 OS cells were seeded on 8-well chambered coverglass (Sarstedt, Germany) that were coated with RGD-modified poly-L-lysine-grafted polyethylene glycol (PLL-PEG-RGD ...
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No products found
because this supplier's products are not listed.
Baptiste Coxam, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... at 56°C for 40min and re-probed with anti–GFP antibody for 1h (1:1000, Origene R1091P). Band intensity was measured using the histogram function on the Fiji software ...
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No products found
because this supplier's products are not listed.
Rajendra Karki, et al.,
bioRxiv - Immunology 2020
Quote:
... caspase-8 (AdipoGen, AG-20T-0138-C100, 1:1000), cleaved caspase-8 CST ...
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No products found
because this supplier's products are not listed.
Annabel Biruete, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Serum levels of the oxidative stress marker 8-hydroxy-2’-deoxyguanosine (8-OHdG) were measured using an ELISA kit (Enzo Life Sciences, Farmingdale, NY)35 ...
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No products found
because this supplier's products are not listed.
Pierrick Bru, et al.,
bioRxiv - Plant Biology 2022
Quote:
... Then incubated for 1h with the secondary goat anti-rabbit antibody conjugated to horse radish peroxidase AS09 602 (1:10,000) from Agrisera in TBST + 3% milk ...
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No products found
because this supplier's products are not listed.
Gina Partipilo, et al.,
bioRxiv - Bioengineering 2021
Quote:
... 2-(4-((bis((1-(tert-butyl)-1H-1,2,3-triazol-4-yl)methyl)amino)methyl)-1H-1,2,3-triazol-1-yl)acetic acid (BTTAA, Click Chemistry Tools > 95%), Click-IT™ Fucose Alkyne (Invitrogen) ...
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No products found
because this supplier's products are not listed.
John Kokkinos, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and caspase 8 (Q-IETD-OPh; Cat. 1176-1, BioVision) were used to treat MiaPaCa2 (20 μM inhibitors) ...
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No products found
because this supplier's products are not listed.
Kirsty J. McMillan, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 2-5 MΩ borosilicate glass electrodes (GC150-10F, Harvard Apparatus) filled with aCSF were placed into the stratum radiatum of a GFP positive region of CA1 and a bipolar stimulating electrode (CBAPB50 ...
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No products found
because this supplier's products are not listed.
Anna C. Deleray, et al.,
bioRxiv - Bioengineering 2023
Quote:
... 2-hydroxyethyl starch (Spectrum Chemical, H3012) was used.
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No products found
because this supplier's products are not listed.
Simon Schäper, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5-bromo-4-chloro-3-indolyl β-d-galactopyranoside (X-Gal, Apollo Scientific) was used at 100 μg/ml ...
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No products found
because this supplier's products are not listed.
Burt M Sharp, Qin Jiang, Panjun Kim, Hao Chen,
bioRxiv - Neuroscience 2023
Quote:
... 2-(3-(4-chloro-3-fluorophenyl)-5-ethyl-1H-1,2,4-triazol-1-yl)-N-(3,5-di-chlorobenzyl)acetamide (MR-L2) was from Targetmol Chemicals ...
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No products found
because this supplier's products are not listed.
Subash Godar, et al.,
bioRxiv - Biophysics 2021
Quote:
... We detected the bound alkaline phosphatase labeled antibodies by incubating the membrane in BCIP/NBT (5-bromo, 4-chloro, 3- indolylphosphate/nitro-blue tetrazolium, AMRESCO, LLC.) substrate for 30–45 minutes ...
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No products found
because this supplier's products are not listed.
Caelan E Radford, et al.,
bioRxiv - Immunology 2023
Quote:
... 1 µL of 10 µM 5’ PacBio round 2 forward primer (PacBio_5pri_RND2), 1 µL of 10 µM 3’ PacBio round 2 reverse primer (PacBio_3pri_RND2) ...
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No products found
because this supplier's products are not listed.
Syed M. Moin, et al.,
bioRxiv - Immunology 2022
Quote:
Purified H10ssF was applied to glow discharged Quantifoil R 2/1 300 mesh grids (Ted Pella, Redding) at a concentration of 1 mg ml-1 ...
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No products found
because this supplier's products are not listed.
Preksha Gupta, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Rainbow Calibration particles (RCP-60-5-2, RCP-60-5-5) were obtained from Spherotech. FITC and PE stained beads were part of the three color BD CaliBRITE kit (BD Biosciences 340486) ...
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No products found
because this supplier's products are not listed.
Santosh Shivakumaraswamy, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 8 ml of 1:1 paraffin oil: silicone oil (Hampton research) was poured before adding 2 μl of protein and 2 μl of the crystallization condition to each well ...
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No products found
because this supplier's products are not listed.
Maximilian W. G. Schneider, et al.,
bioRxiv - Cell Biology 2021
Quote:
... was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 μl of 5 mg ml−1 Biotin-XX-labeled tubulin (Pursolutions ...
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No products found
because this supplier's products are not listed.
Imke Ensinck, et al.,
bioRxiv - Biochemistry 2022
Quote:
... and 100uL of primary antibody solution was added and incubated for 1h at room temperature (1:10000 Abclonal A19841 including 0.5ug/mL competing ime4Δ yeast total RNA) ...
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No products found
because this supplier's products are not listed.
Luise A. Seeker, et al.,
bioRxiv - Neuroscience 2022
Quote:
... washed again (2 x 5 min) and incubated with OPAL 650 Tyramide (Akoya Biosciences, FP1496001KT, 1:500) for 10 minutes ...
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No products found
because this supplier's products are not listed.
Manish Kuchakulla, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Code: AAH-CYT-5-2 (RayBiotech, Norcross, GA, USA) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Carlos J. Díaz Osterman, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... cells were seeded in poly-hydroxyethyl methacrylic acid (poly-HEMA) coated Costar plates (non-adherent) in serum-free CSC media (3D Tumorsphere Medium XF, PromoCell GmbH) at dilutions recommended by the manufacturer ...
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No products found
because this supplier's products are not listed.
Maral Baghai Arassi, et al.,
bioRxiv - Microbiology 2024
Quote:
... DNA was fragmented into 2–8 kb pieces using focused ultrasonication (Covaris E220 with miniTUBE red). DNA Fragments were subsequently cloned into a PCR-linearized pZE21 expression vector (primers 1 and 2 ...
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No products found
because this supplier's products are not listed.
Takashi Nishina, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Takagi47 and R-spondin 1-CM (final concentration of 10%) (Trevigen).
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No products found
because this supplier's products are not listed.
Yi Juin Liew, et al.,
bioRxiv - Neuroscience 2021
Quote:
Tungsten microelectrodes (FHC, impedance: 2-5 MΩ, 75 µm in diameter) were used in thalamus and cortex to isolate single units that were responding to a single primary whisker on the contralateral side of the face ...
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No products found
because this supplier's products are not listed.
Geoffrey A. Smith, et al.,
bioRxiv - Genomics 2021
Quote:
... and the appropriate pLKO-derived vector (at ratios of 8 µg, 1 µg, and 8 µg, respectively, per 15 cm dish) with Trans-LT1 (Mirus Bio, Madison WI), according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Ming Zhang, et al.,
bioRxiv - Neuroscience 2021
Quote:
... CCNB1 (Boster BA0766-2, 1:500), β-actin (Proteintech 66009-1,1:5000) ...
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No products found
because this supplier's products are not listed.
Jun Sun, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Rabbit anti-5-HT 1:500 (Immunostar); Rabbit Anti-DCP-1 1:250 (Cell Signalling) ...
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No products found
because this supplier's products are not listed.
Min Hu, et al.,
bioRxiv - Biochemistry 2020
Quote:
... 10 μL CCK-8 (Solarbio) was added to a 96-well plate ...
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No products found
because this supplier's products are not listed.
Vivian K. Rojas, et al.,
bioRxiv - Microbiology 2024
Quote:
... The chromogenic molecule X-Phos (5-bromo-4-chloro-3-indolyl phosphate, Chem-Impex) was added to agar plates with the purpose of detecting S ...
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No products found
because this supplier's products are not listed.
Aude Noiret, Fabienne Aujard, Jeremy Terrien,
bioRxiv - Evolutionary Biology 2023
Quote:
... ref MS E-5100) and 8-hydroxy-2’-deoxyguanosine (“8-OHdG,” in ng.ml− 1; OxiSelectTM Oxidative DNA Damage Elisa kit, Cell Biolabs Inc., ref STA-320) were measured in duplicates from urine samples as indicators of the organisms’ response to environmental stress (Miller et al. ...
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No products found
because this supplier's products are not listed.
Aya Ghassani, et al.,
bioRxiv - Microbiology 2023
Quote:
... VII-1-1 and IX-5-2 (Genomic DNA extraction kit, Macherey-Nagel, Hoerdt) by using the primers listed in Table S4 ...
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No products found
because this supplier's products are not listed.
Megan B. Borror, et al.,
bioRxiv - Pathology 2019
Quote:
R-loop formation in worms was quantified using αS9.6 primary antibody (Kerafast, 1:2000 in 5% Blotto, 16 hours, 4°C), previously shown to be selective for DNA::RNA hybrids 50 ...
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No products found
because this supplier's products are not listed.
Jillian M. DiMuzio, et al.,
bioRxiv - Immunology 2021
Quote:
... 8×106 293TN Producer cells (System Biosciences, Cat # LV900A-1) were plated in T150 flasks overnight ...
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No products found
because this supplier's products are not listed.
Gregory J. Ordemann, et al.,
bioRxiv - Neuroscience 2021
Quote:
Extracellular stimulation was performed using bipolar sharp tungsten electrodes (5 MΩ, 8° taper, A-M Systems) connected to a Neurolog NL800A current stimulus isolator (Digitimer) ...
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No products found
because this supplier's products are not listed.
Marine Theret, et al.,
bioRxiv - Cell Biology 2021
Quote:
C3H10T1/2 is a cell line of mesenchymal progenitors (Clone 8, American Type Culture Collection, Manassas, USA), the cells need to be maintained at 50-70% confluence ...
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No products found
because this supplier's products are not listed.
Jungmin Lee, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... Supernatant was concentrated to 5–8 mL using a 10 kDa cut-off Macrosep Advance centrifugal device (Pall). Concentrated protein was bound to 0.5–1 mL of His60 nickel or HisTalon cobalt resin (Takara Bio ...
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No products found
because this supplier's products are not listed.
Marta Vranas, et al.,
bioRxiv - Biochemistry 2021
Quote:
... and 1 mM 5-FAM-Lysine (Anaspec), prepared from a 20 mM stock in DMSO ...
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No products found
because this supplier's products are not listed.
Lasse Toftdal Dynesen, et al.,
bioRxiv - Microbiology 2023
Quote:
... Concentrated proteins were resuspended in buffer W at pH 8 and incubated with BioLock (#2-0205-050, Iba Life sciences) for 15 min at RT to neutralize biotin ...
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No products found
because this supplier's products are not listed.
Oliver D Caspari, et al.,
bioRxiv - Plant Biology 2022
Quote:
... selected for Paromomycin resistance were grown in 200μl TAP in 96-well plates under 50μmol photons m-2 s-2 for 3-5 days and then screened for Venus expression in a fluorescence plate reader (CLARIOstar, BMG Labtech) as described previously (Caspari ...
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No products found
because this supplier's products are not listed.
Chujun Zhang, et al.,
bioRxiv - Cell Biology 2021
Quote:
... and the fluorescence intensity of the dye was immediately recorded over a period of 1h at 26°C using a Spark multimode microplate reader (Tecan) with an excitation of 480nm and an emission of 530nm ...