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No products found
because this supplier's products are not listed.
Lars Kaiser, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... Caspase 3/7 activation was measured using the Apo-ONE Homogenous Caspase-3/7 Assay (Promega), following the manufacturers protocol ...
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No products found
because this supplier's products are not listed.
Evan R. Semenza, et al.,
bioRxiv - Neuroscience 2021
Quote:
... and 6-chloro-1,2-benzisoxazol-3(2H)-one (CBIO) were purchased from Sigma Aldrich. Phorbol 12-myristate 13-acetate was purchased from Cayman Chemical ...
-
No products found
because this supplier's products are not listed.
Muhammad Junaid Yousaf, et al.,
bioRxiv - Physiology 2019
Quote:
... and HDMBOA (4,7-dimethoxy-2-{[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}-3,4-dihydro-2H-1,4-benzoxazin-3-one)) (Block et al., 2019) (Yang et al., 2019) were quantified using HPLC (Thermofisher scientific) which was coupled with MS (UltiMate 3000 HPLC ...
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No products found
because this supplier's products are not listed.
Soraya Villaseca, et al.,
bioRxiv - Cell Biology 2023
Quote:
... ζ-PKC (Santa Cruz, (B-7), sc-393218 ...
-
No products found
because this supplier's products are not listed.
Yan Tang, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... the small molecule 3-[2-[(4-Fluorophenyl)methyl]imidazo[2,1-b]thiazol-6-yl]-2H-1-benzopyran-2-one (iMDK; TOCRIS Bio-techne, 9mg/kg), rapamycin (Sirolimus A8167 ...
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No products found
because this supplier's products are not listed.
MG Booty, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... Caspase 3/7 indicator dye (Sartorius, Germany) and propidium iodide (BD Biosciences ...
-
No products found
because this supplier's products are not listed.
Samuel J. Resnick, et al.,
bioRxiv - Microbiology 2020
Quote:
... AZVIII-40A (1,2-Benzisothiazol-3(2H)-one) was purchased from Alfa Aesar. Other protease inhibitors in table below were purchased from TargetMol.
-
No products found
because this supplier's products are not listed.
Jack Stubbs, et al.,
bioRxiv - Biochemistry 2024
Quote:
... a volume of 1H,1H,2H,2H-perfluoro-1-octanol (PFO, Merck) is added to the emulsion with gentle pipetting used to break the emulsion ...
-
No products found
because this supplier's products are not listed.
Jennifer G. Abelin, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... 3 µL B-K27 (Abcam, ab47342). The HLA complexes were captured on the beads by incubating on a rotor at 4°C for 3hr ...
-
No products found
because this supplier's products are not listed.
Christopher Wong, Elena M. Jurczak, Richard Roy,
bioRxiv - Developmental Biology 2023
Quote:
... rgef-1p and gfp were inserted into a vector containing the sequence rab-7 amplified using PCR and primers 5’-atgtcgggaaccagaaagaa-3’ and 3’-aagcttatcgataccgtcgac-5’ to create rgef-1p::gfp::rab-7::rab-7 3’UTR using Gibson assembly (NEB E2611). A full list of reagents and resources can be found in table S2.
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No products found
because this supplier's products are not listed.
Teresa Neuwirth, et al.,
bioRxiv - Immunology 2024
Quote:
... Each patient was also stained with one of TotalSeq™-C0251/2/3/4 Antibody (Biolegend, Cat: 394661/3/5/7, 1:100) for sample multiplexing ...
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No products found
because this supplier's products are not listed.
Sapir Herchcovici Levi, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... 3 µM CHIR99021 (PeproTech, SM-2520691-B) and 0.2 µM PD0325901 (PeproTech ...
-
No products found
because this supplier's products are not listed.
Yiru Long, et al.,
bioRxiv - Immunology 2024
Quote:
... B cells were collected and stained with 5 μL of 7-AAD (559925, BD Pharmingen) at room temperature for 10 min ...
-
No products found
because this supplier's products are not listed.
Jose A. Valverde-Lopez, et al.,
bioRxiv - Developmental Biology 2023
Quote:
FLICA™ 660 Caspase-3/7 (BIORAD) was used to measure apoptosis according to manufacturers.
-
No products found
because this supplier's products are not listed.
Eleni Kafkia, et al.,
bioRxiv - Systems Biology 2020
Quote:
... the nuclei pellet was resuspended in 3 mL buffer consisted of one part buffer B and two parts buffer C (2.3M sucrose ...
-
No products found
because this supplier's products are not listed.
Peyton J. Spreacker, et al.,
bioRxiv - Biophysics 2022
Quote:
... 2H-54-DMPC and 2H-22-DHPC (Avanti Polar Lipids, Alabaster, AL) were used to minimize the lipid background in the methyl region of the NMR spectra.
-
No products found
because this supplier's products are not listed.
Oliver Johanndrees, et al.,
bioRxiv - Plant Biology 2021
Quote:
... Four-mm leaf discs from 4th or 5th leaves of 5-week-old Nb plants were washed in double-distilled (mQ) water for 2h and incubated in 200 μl of mQ water in 96-well plates (Greiner Bio-One, #655075) under aluminum foil overnight ...
-
No products found
because this supplier's products are not listed.
Tonci Ivanisevic, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... or hygromycin B for 7 days (500 µg/ml, InvivoGen). Cells were transfected using GeneJuice (Merck ...
-
No products found
because this supplier's products are not listed.
Theodoros Karantanos, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay (11465007001, Roche Diagnostics, Mannheim, Germany) was performed according to manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Christopher Douglas, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... HGF (5’-CTCACACCCGCTGGGAGTAC-3’, 5’-TCCTTGACCTTGGATGCATTC-3’), STAT3 (5’-CTTTGAGACCGAGGTGTATCACC-3’, 5’-GGTCAGCATGTTGTACCACAGG-3’) and B-Actin Primer Set (Qiagen, QT00095431). After preparing master mixes samples were prepared in quadruplicate in a 96-well Reaction Microplates (Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Tuanjun Hu, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... AP20187 (B/B) compound (Takara Bioscience) was injected into the dorsal aorta through a small window made in the ventral egg ...
-
Cat# HY-33900-500 mg,
500 mg, USD $50.0
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Isadora Oliveira Prata, et al.,
bioRxiv - Microbiology 2021
Quote:
1-(2,3-di(Thiophen-2-yl)quinoxalin-6-yl)-3-(2-methoxyethyl)urea (CAS 508186-14-9 – MCE-MedChemExpress) is an Acetyl-CoA Synthetase 2-specific and potent inhibitor ...
-
No products found
because this supplier's products are not listed.
Olivia T.M. Bucheli, et al.,
bioRxiv - Immunology 2023
Quote:
The number of B cells secreting IgG was quantified using a mouse ELISpot kit (Mabtech, 3825-2H). The isolated cells were counted using the Countess Automated Cell Counter II (ThermoFisher ...
-
No products found
because this supplier's products are not listed.
Vicky Katopodi, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... and Caspase 3/7 (1:300) [Cleaved Caspase-3 (Asp175) (5A1E) (Cell Signaling)] ...
-
No products found
because this supplier's products are not listed.
Emily C. Britt, Jing Fan,
bioRxiv - Biochemistry 2020
Quote:
... and 3-2H-glucose (all from Cambridge Isotope), the isotopically labeled glucose was substituted for unlabeled glucose at the same concentration in RPMI culture media ...
-
No products found
because this supplier's products are not listed.
D Singh, et al.,
bioRxiv - Immunology 2020
Quote:
... anti-granzyme B (GrB-7; 1:50; Dako) and anti-PanCK (AE1/AE3 ...
-
No products found
because this supplier's products are not listed.
Paula Tucci, et al.,
bioRxiv - Microbiology 2019
Quote:
... Proteins were loaded into 7 cm IPG Strips 3-10 (GE Healthcare) by overnight passive rehydration ...
-
No products found
because this supplier's products are not listed.
Huda Jumaa, et al.,
bioRxiv - Immunology 2021
Quote:
... 1×105 transformed or 3×105 normal B cell precursors or 106 mature stimulated B cells were plated on Cell-Tak (Corning) coated Seahorse cell culture plates ...
-
No products found
because this supplier's products are not listed.
Robert Schönherr, et al.,
bioRxiv - Biophysics 2023
Quote:
... at 40 °C and for 7 min in 3 % lead citrate (Ultrostain II, Leica) at 20 °C ...
-
No products found
because this supplier's products are not listed.
Kehui Xiang, David P. Bartel,
bioRxiv - Molecular Biology 2021
Quote:
... laevis b-globin 5′- and 3′-UTR sequences were obtained from pT7TS (Addgene #17091). Mouse Malat1 3′ sequence was obtained from the Comp.25 mutant plasmid (Wilusz et al. ...
-
No products found
because this supplier's products are not listed.
Evelyne Krin, et al.,
bioRxiv - Microbiology 2023
Quote:
... 3-(2,4-dinitrostyryl)-(6 R,7 R)-7-(2-thienylacetamido)-ceph-3-em-4-carboxyl acid (Calbiochem), was used to assess membrane permeability ...
-
No products found
because this supplier's products are not listed.
Chahrazade Kantari-Mimoun, et al.,
bioRxiv - Immunology 2020
Quote:
Cell death within tumor slices was assessed using a green-fluorescent caspase 3/7 probe that binds DNA upon cleavage by caspase 3/7 (Nucview, Biotium). 2.106 untransduced or EGFR CAR T cells were applied onto BxPC3 tumor slices that were subsequently incubated with 5μM of NucView dye ...
-
No products found
because this supplier's products are not listed.
Hongchen Shen, et al.,
bioRxiv - Microbiology 2021
Quote:
... and a dye photosensitizer in N,N-dimethylformamide/acetone (7/3, v/v) was electrospun onto one layer of polypropylene (PP) fabrics (VWR® Basic Protection Face Mask). During the electrospinning of 10-20 wt% of PVDF ...
-
No products found
because this supplier's products are not listed.
Jhimmy Talbot, et al.,
bioRxiv - Immunology 2019
Quote:
... Tissue was washed and when needed incubated with secondary fluorescently labeled antibodies (Donkey Anti-Rabbit Pacific Blue or Alexa Fluor 647) for 2h before nuclear staining with Draq-7 (R&D Systems) or 4′,6-diamidino-2-phenylindole (DAPI ...
-
No products found
because this supplier's products are not listed.
Gregory W. Busey, et al.,
bioRxiv - Immunology 2023
Quote:
... and a FlexStation 3 plate reader using SoftMax Pro 7 (Molecular Devices).
-
No products found
because this supplier's products are not listed.
Matthew Waas, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Cells were cultured for 7 d at 5% O2 in EpiCult- B mouse medium (STEMCELL Technologies, 05610) supplemented with 5% FBS ...
-
No products found
because this supplier's products are not listed.
Christopher Jonkergouw, et al.,
bioRxiv - Biochemistry 2023
Quote:
... N-(3-Hydroxy-7-cis-tetradecanoyl)-L-HSL (>95%) (3-OH-C14:1) was used as received from Cayman Chemical. Organic solvents were purchased from Sigma-Aldrich and VWR ...
-
No products found
because this supplier's products are not listed.
JS Cho, et al.,
bioRxiv - Pathology 2024
Quote:
... Isotype controls or fluorescence minus one (FMO) controls were used, and the live/dead marker 7-aminoactinomycin D (7-AAD, PerCP) (Miltenyi Biotec) was added to all samples 10 min before analysis (10 μl to 1 ml of cell suspension).
-
No products found
because this supplier's products are not listed.
May Zaw Thin, et al.,
bioRxiv - Cell Biology 2019
Quote:
... 3 and 7 after renal artery injection using an IVIS Lumina (PerkinElmer, USA). Mice were injected intraperitoneally with 75 mg/kg D-luciferin (Promega ...
-
No products found
because this supplier's products are not listed.
Igor A. Gak, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Gradients were then centrifuged for 2h 45 min on 35.000 rpm (Beckman Coulter ...
-
No products found
because this supplier's products are not listed.
Harald Hartweger, et al.,
bioRxiv - Immunology 2019
Quote:
... washed once in PBS and resuspended in Mouse B cell Nucleofector Solution with Supplement (murine B cells) or Primary Cell Nucleofector Solution 3 with Supplement (human B cells) prepared to the manufacturer’s instructions (Lonza) at a concentration of 4 - 5 × 106 cells / 86 μL ...
-
No products found
because this supplier's products are not listed.
Aikaterini Kalamari, et al.,
bioRxiv - Animal Behavior and Cognition 2020
Quote:
... except for 7 pairs of males from pilot experiment B that originated directly from Charles River. Only male rats were included in the experiments ...
-
No products found
because this supplier's products are not listed.
Megha Maheshwari, et al.,
bioRxiv - Neuroscience 2023
Quote:
... for 2h followed by incubation with avidin-biotin complex (Vector Laboratories) for 1h ...
-
No products found
because this supplier's products are not listed.
Soma Szentkirályi-Tóth, et al.,
bioRxiv - Neuroscience 2023
Quote:
... biotin-conjugated anti-mouse IgG (Jackson ImmunoResearch Laboratories; 1:500; 2h; RT), ABC Elite reagent (Vector ...
-
No products found
because this supplier's products are not listed.
Benjamin Craske, et al.,
bioRxiv - Biochemistry 2022
Quote:
... microtubule seeds containing 7% rhodamine-tubulin (Cytoskeleton Inc., TL590M-B,) were polymerised in BRB80 (80 mM PIPES pH 6.9 ...
-
No products found
because this supplier's products are not listed.
Yuanyuan Yu, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Puromycine (1 μg/ml,2h, GoldBio), α-Amanitin (5 μg/ml ...
-
No products found
because this supplier's products are not listed.
Philippe Jean, et al.,
bioRxiv - Neuroscience 2020
Quote:
Patch-pipettes (3-7 MOhm in bath) were pulled (P87 puller, Sutter Instruments, USA) from borosilicate glass capillaries (GB150F-8P and GB150-8P Science Products ...
-
No products found
because this supplier's products are not listed.
Christopher B. Kaelin, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Libraries were constructed with version 2 (stage 16a) or version 3 (stage15 a/b) chemistry and sequenced on a single HiSeqX (Illumina) lane to generate 400-450 million paired-end 150 bp reads (Supplementary Table 4).
-
No products found
because this supplier's products are not listed.
Ayantika Sen Gupta, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Images for co-labelled CENP-A and CENP-B FISH probes were obtained using the Lattice SIM technology on the Elyra 7 microscope (Carl Zeiss AG) with a similar setup ...
-
No products found
because this supplier's products are not listed.
The International Brain Laboratory, et al.,
bioRxiv - Neuroscience 2020
Quote:
Animals (all female and male C57BL6/J mice aged 3-7 months obtained from Jackson Laboratory or Charles River) were co-housed whenever possible ...