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No products found
because this supplier's products are not listed.
Aidan McGlinchey, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 3β-Hydroxy-5-cholestene-3-linoleate (ChoE(18:2)) from Larodan, were prepared to the following concentration levels ...
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No products found
because this supplier's products are not listed.
Subash Godar, et al.,
bioRxiv - Biophysics 2021
Quote:
... We detected the bound alkaline phosphatase labeled antibodies by incubating the membrane in BCIP/NBT (5-bromo, 4-chloro, 3- indolylphosphate/nitro-blue tetrazolium, AMRESCO, LLC.) substrate for 30–45 minutes ...
-
No products found
because this supplier's products are not listed.
Pazhanichamy Kalailingam, et al.,
bioRxiv - Pathology 2024
Quote:
... then incubated overnight at 37°C with staining buffer (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside [X-gal, Bioline, London ...
-
No products found
because this supplier's products are not listed.
Ryan A.V. Bell, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... and recombinant active caspase 3 (0.5 μg; Chemicon) or recombinant active caspase 7 (0.5 μg; Biovision) were incubated for 3 h in cleavage assay buffer (50 mM Hepes ...
-
No products found
because this supplier's products are not listed.
Shail Kabrawala, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Actin (2µM; 7% pyrene-labeled) was then polymerized in the presence of 20nM bovine Arp2/3 complex (Cytoskeleton Inc.) plus MBP-tagged fusion proteins in control buffer supplemented with 0.2mM ATP and 0.5mM DTT ...
-
No products found
because this supplier's products are not listed.
Yun-Seok Choi, et al.,
bioRxiv - Biochemistry 2019
Quote:
... cells were incubated overnight at 4 °C with anti-HA antibody (Sigma-Aldrich clone HA-7 or Bethyl Laboratories A190-108A ...
-
No products found
because this supplier's products are not listed.
Zhen-lu Li, et al.,
bioRxiv - Biophysics 2020
Quote:
1μg of recombinant Plexin-B1 FL WT pCDNA 3.1 plasmid or Plexin-B1 FL mutants pCDNA 3.1 (as described above) was transfected to 80% confluent COS 7 cells on coverslips using 3 μL Trans IT 2020 transfection reagent (Mirus Bio) for 48 hours at 37 °C ...
-
No products found
because this supplier's products are not listed.
Mark A Gutierrez, Brett E Dwyer, Santos J Franco,
bioRxiv - Neuroscience 2020
Quote:
... Samples were incubated for 3 hours at 4°C with Anti-DYDDDDK Affinity Gel (Rockland, RRID:AB_10704031). After incubation ...
-
No products found
because this supplier's products are not listed.
Tomasz Czerniak, James P Saenz,
bioRxiv - Biochemistry 2021
Quote:
... 7-deaza-GTP (Trilink Biotechnologies, USA) was used ...
-
No products found
because this supplier's products are not listed.
Dalia E. Gaddis, et al.,
bioRxiv - Immunology 2019
Quote:
... Nrp1 (clone N43-7; MBL International, Woburn, MA), and CD45.1 (clone A20 ...
-
No products found
because this supplier's products are not listed.
Lucas E. Cabrera Zapata, et al.,
bioRxiv - Neuroscience 2022
Quote:
... ChIP reactions were carried out overnight at 4 °C with 3 µg of sheared chromatin and 10 μg of anti-H3K27me3 rabbit polyclonal antibody (39155, Active Motif) or anti-mouse IgG as negative control (115-001-003 ...
-
No products found
because this supplier's products are not listed.
Diogo Tavares, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
... in a rotating wheel (TC-7, New Brunswick/Eppendorf, Belgium). The next day ...
-
No products found
because this supplier's products are not listed.
Chrysa Koukorava, et al.,
bioRxiv - Cell Biology 2023
Quote:
NHDFs (passage 4-10) were seeded at 3 x 104/well on gelatinised glass-bottomed 24-well plates (662892; Greiner Bio-One) in FGM and cultured for 3 days ...
-
No products found
because this supplier's products are not listed.
Wander Van Breedam, et al.,
bioRxiv - Bioengineering 2021
Quote:
... from Bio-Rad (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels ...
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No products found
because this supplier's products are not listed.
Priyanka Nain, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... 3-(4-hydroxy-3-methoxyphenyl) propanal was procured from AA Blocks. 3-(4-Hydroxy-3-methoxyphenyl ...
-
No products found
because this supplier's products are not listed.
Rinaldo D. D’Souza, et al.,
bioRxiv - Neuroscience 2020
Quote:
... into one of ten cortical areas and performing iontophoretic injections (3 µA, 7 s on/off cycle for 7 minutes; Midgard current source; Stoelting) of biotinylated dextran amine (BDA ...
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No products found
because this supplier's products are not listed.
Reza Nejat, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... Ubiquitin−7-amino-4-methylcourmarin (Ub-AMC) was purchased from Boston Biochem; human ISG15−7-amino-4-methylcourmarin (ISG15-AMC ...
-
No products found
because this supplier's products are not listed.
Camille Mazo, et al.,
bioRxiv - Neuroscience 2022
Quote:
... An array of 4 tungsten electrodes (∼3 MΩ; FHC) was glued together and was slowly lowered into the OB ...
-
No products found
because this supplier's products are not listed.
Kawsar Hossain, et al.,
bioRxiv - Neuroscience 2024
Quote:
... incubated with EdU reaction solution (4 mM CuSO4, 4 µM Sulfo-Cyanine 3 Azide [Lumiprobe] ...
-
No products found
because this supplier's products are not listed.
Arpit Kumar Pradhan, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 2 ng of epietiocholanolone (3β-hydroxy-5β-androstan-17-one, Steraloids, Newport, Rhode Island) for 5α/β-dihydrotestosterone (DHT) ...
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No products found
because this supplier's products are not listed.
Eric Hee Jun Lee, et al.,
bioRxiv - Cancer Biology 2023
Quote:
Tumor tissue was fixed for up to 3 days in 4% paraformaldehyde (4% PFA, Boston BioProducts) and stored in 70% ethanol until further processing ...
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No products found
because this supplier's products are not listed.
Hao Guo, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Germany) with a guard column (EC 4/3 NUCLEODUR C18 Gravity, 3 μm, Macherey-Nagel GmbH & Co. KG Germany), while the column temperature was kept at 40 °C in a thermostatic column compartment (Thermo Fisher UltiMate 3000) ...
-
No products found
because this supplier's products are not listed.
Shuntaro Morikawa, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Fluorescence for cell viability and luminescence for caspase-3/7 activity was measured using Infinite M1000 plate reader (Tecan). Caspase-3/7 activity was normalized to cell viability according to the manufacturer’s protocol.
-
No products found
because this supplier's products are not listed.
Sang Soo Kim, et al.,
bioRxiv - Neuroscience 2019
Quote:
... GT1-7 cells were infected with the retrovirus encoding MC4R-3×HA using ViraDuctin™ Transduction Kit (Cell Biolabs, Inc.) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Yuchen Li, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Organoid sections (4 µm) were probed with anti-cleaved caspase 3 (Biocare Medical) in 10% goat serum for 2 hours at room temperature and subsequently stained with Mach 1 Universal Polymer HRP ...
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No products found
because this supplier's products are not listed.
Matthias Fellner, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Caspase 3-like activity was measured fluorometrically by the addition of 50 µM acetyl-Asp-Glu-Val-Asp-7-amino-4-methylcoumarin (Ac-DEVD-AMC) at 37 °C in 96-well plates using a ClarioSTAR microplate reader (BMG LABTECH). The production of AMC (λEM = 390 nm ...
-
No products found
because this supplier's products are not listed.
Shirsendu Ghosh, et al.,
bioRxiv - Biophysics 2020
Quote:
7) Glass bottom culture dish (MatTek, 35 mm petri dish ...
-
No products found
because this supplier's products are not listed.
Bogdan B. Grigorash, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Keratin 7 (Genetex #GTX110414; 1:250), Keratin 8 (DSHB Hybridoma Product TROMA-I ...
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No products found
because this supplier's products are not listed.
Steffen Klein, et al.,
bioRxiv - Cell Biology 2020
Quote:
... for 4 weeks (the medium was exchanged 3 times a week) and Detach Kit (PromoCell, C-41210) was used to detach the cells from the culture dish before seeding the cells on EM grids ...
-
No products found
because this supplier's products are not listed.
Yue Qu, et al.,
bioRxiv - Neuroscience 2021
Quote:
The completed constructs in M-6-attB-UAS-1-3-4 vector were amplified in Epi300 competent cells (EpiCentre) in LB-Chloramphenicol medium ...
-
No products found
because this supplier's products are not listed.
Ryann M. Fame, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 5–10 μL of CSF or serum was extracted in 4:6:3 chloroform:methanol:water mixture supplemented with isotopically labeled T3 and T4 (at 100 nM, Cambridge Isotope Laboratories ...
-
No products found
because this supplier's products are not listed.
Hélène Scheer, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 10 pmol of gene-specific primer (Supplementary Table 7) and 10 pmol of a TruSeq RNA PCR index (RPI, Supplementary Table 7) 10 nmol of dNTP ...
-
No products found
because this supplier's products are not listed.
Najet Serradj, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The double layer of dorsal musculature was closed with 7-0 vicryl absorbable surgical suture (Ethicon) and skin closed with 7 mm surgical wound clips (Fine Science Tools). Mice were given 1 ml saline (0.9% solution ...
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No products found
because this supplier's products are not listed.
Anastasia Accoti, et al.,
bioRxiv - Microbiology 2023
Quote:
... re-suspended in water was injected into the thorax of cold-anesthetized 3-4 day old female mosquitoes using a Nanoject III (Drummond Scientific).
-
No products found
because this supplier's products are not listed.
Amador Romero-Franco, et al.,
bioRxiv - Cell Biology 2024
Quote:
... membranes were blotted with the appropriate primary antibody (Suppl. Table 3) and infrared dyed secondary antibodies (Suppl. Table 4) diluted in Odyssey Blocking Buffer (LI-COR) supplemented with 0.1% Tween-20 and 0.01% Sodium Azide ...
-
No products found
because this supplier's products are not listed.
Julius Brandenburg, et al.,
bioRxiv - Immunology 2020
Quote:
... and incubated for 7 days in cell culture dishes (Sarstedt, Nümbrecht, Germany) in DMEM containing 10 mM HEPES ...
-
No products found
because this supplier's products are not listed.
Donatas Repecka, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
... and MultiQuant 3 (Sciex) was used for analysis and quantitation of results ...
-
No products found
because this supplier's products are not listed.
Ashwathi Rajeevan, Riya Keshri, Sachin Kotak,
bioRxiv - Cell Biology 2020
Quote:
Double-stranded siRNA oligonucleotides used were 5’- CAGUACCAGUGAGUGGCCCCACCUG-3’ (NuMA 3’UTR siRNA; Eurogentec) and 5’-CACCGUGUGUCUAAGCAAA-3’ (RCC1 siRNA ...
-
No products found
because this supplier's products are not listed.
Musleh M. Muthana, et al.,
bioRxiv - Immunology 2023
Quote:
... Polyhistidine-tagged human CTLA-4 (HIS-hCTLA-4) (Sino Biological Inc, 11159-H08H). Mouse TNF-alpha (Sino Biological Inc ...
-
No products found
because this supplier's products are not listed.
Simon Schäper, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5-bromo-4-chloro-3-indolyl β-d-galactopyranoside (X-Gal, Apollo Scientific) was used at 100 μg/ml ...
-
No products found
because this supplier's products are not listed.
Andrew Barszczyk, et al.,
bioRxiv - Cell Biology 2023
Quote:
... alkaline phosphatase conjugated antibodies were detected colourometrically using BCIP/NBT (nitro-blue tetrazolium and 5- bromo-4-chloro-3’-indolyphosphate) Substrate Solution (#BE116.100ML; Bio Basic Canada Inc). For analysis of bands ...
-
No products found
because this supplier's products are not listed.
Sherine E. Thomas, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Wizard 3&4 (Molecular Dimensions), JCSG +Suite (Molecular Dimensions) ...
-
No products found
because this supplier's products are not listed.
W. Grant Ludlam, et al.,
bioRxiv - Biochemistry 2019
Quote:
The BBS2-7-9 complex was purified at 4°C by affinity purification using 4 mL of Strep-Tactin resin (IBA Life Sciences) packed in a 2 cm diameter column and equilibrated with equilibration buffer (20 mM HEPES pH 7.5 and 20 mM NaCl) ...
-
No products found
because this supplier's products are not listed.
Amada M. Abrego, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Rats were induced with 3-4% isoflurane (SomnoSuite, Kent Scientific) and all whiskers on the right facial pad were trimmed except B1 ...
-
No products found
because this supplier's products are not listed.
Simon Amiard, et al.,
bioRxiv - Plant Biology 2023
Quote:
... and chromatin sonicated using the Diagenode Bioruptor (set to high intensity, 3 times 7 cyles (30sec ON / 30 sec OFF) or the S220 Focused-ultrasonicator (Covaris) for 20 min at peak power 110 W ...
-
No products found
because this supplier's products are not listed.
Tanay Ghosh, et al.,
bioRxiv - Neuroscience 2022
Quote:
... in QuantStudio 7 Flex (Applied Biosystems, ABI) machine ...
-
No products found
because this supplier's products are not listed.
Christiane Geithe, et al.,
bioRxiv - Biochemistry 2021
Quote:
We used hybriwell chambers (16 wells, 7 mm x 7 mm x 0.05 mm; RD477991, Grace Bio-Labs, Oregon, USA) that were customized according to our needs ...
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35 mm glass bottom dish with 4 chambers, 20mm microwell, #1 cover glass (0.13-0.16mm). Designed...
Cat# D35C4-20-1-N,
100/case, $184.00
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Lissenya B. Argueta, et al.,
bioRxiv - Cell Biology 2021
Quote:
... hESC-qualified)-coated plates at 4×10^5/well in 24-well plates or 3×10^4/well in glass-like polymer bottom 96-well plates (CellVis).
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No products found
because this supplier's products are not listed.
Shahzad S. Khan, et al.,
bioRxiv - Cell Biology 2021
Quote:
... the other group (7 mice) received untreated diet (Research Diets D01060501) for 14 days and served as the control group ...
-
No products found
because this supplier's products are not listed.
Abhishek Jamwal, et al.,
bioRxiv - Microbiology 2022
Quote:
... ft tangential filter flow unit (3 kDa, PALL). The buffer-exchanged supernatant was loaded onto a CaptureSelect™ C-tagXL pre-packed column (Thermofisher Scientific) ...