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No products found
because this supplier's products are not listed.
Aya Ghassani, et al.,
bioRxiv - Microbiology 2023
Quote:
... VII-1-1 and IX-5-2 (Genomic DNA extraction kit, Macherey-Nagel, Hoerdt) by using the primers listed in Table S4 ...
-
No products found
because this supplier's products are not listed.
Kevin W. Graepel, et al.,
bioRxiv - Microbiology 2019
Quote:
... genomic RNA was detected with a 5’ 6-carboxyfluorescein (FAM) and 3’ black hole quencher 1 (BHQ-1) labeled probe targeting nsp2 (Biosearch Technologies, Petaluma, CA), and RNA copy number was calculated by reference to an RNA standard derived from the MHV A fragment ...
-
No products found
because this supplier's products are not listed.
Maria Zimmermann-Kogadeeva, et al.,
bioRxiv - Systems Biology 2022
Quote:
... and lysed in a bead beater high for 4 min (2 min / kept on ice 1 min / 2 min) (BioSpec Products). Cellular debris was removed by centrifugation (8,000 × g ...
-
No products found
because this supplier's products are not listed.
Agata Stepien, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... rat antibodies for the detection of phosphorylated RNAPII (serine 5 and serine 2) (Chromotek, 1: 100) and secondary goat anti-rat Alexa Fluor 488 antibodies (A-11006 ...
-
No products found
because this supplier's products are not listed.
Daniel Bsteh, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... for 6 cycles (1 min on/1 min off) on a Bioruptor Pico sonicator (Diagenode). For each ChIP reaction 4 % HEK293T-derived human spike-in lysate was combined with mESC lysate and incubated in 1x IP buffer (50 mM HEPES/KOH pH 7.5 ...
-
No products found
because this supplier's products are not listed.
Charlotte H. Hurst, et al.,
bioRxiv - Plant Biology 2023
Quote:
... Calnexin 1/2 (Agrisera AS12 2365) and UDP-glucose pyrophosphorylase (Agrisera AS05 086 ...
-
No products found
because this supplier's products are not listed.
Andrew M. Lerner, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... H3K36me3 (Abcam 9050, 1:1000 for ECL, 1:2000 for LI-COR and 2 μL for ChIP), H3K36me2 (Active Motif 38255 ...
-
No products found
because this supplier's products are not listed.
P. Lejeune, et al.,
bioRxiv - Plant Biology 2020
Quote:
... Jiffypots® with weak or abnormal plantlets were discarded and the others were transplanted into 12-cm square plastic cultivation pots filled with 1.5 L of leaf mould and baked clay (4:1) mixed with 6 gr.L−1 of slow release fertilizer (Osmocote Exact Standard 5-6 M, ICL Specialty Fertilizers). The pots were fitted at the bottom with a 2 x 10 cm felt wick and randomly placed on the deck of the cultivation gutters described above ...
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No products found
because this supplier's products are not listed.
Jonathan Tai, et al.,
bioRxiv - Biochemistry 2023
Quote:
... [1-14C]-IPP and [phenyl-14C]-4-HB were purchased from American Radiolabeled Chemicals. At each time point ...
-
No products found
because this supplier's products are not listed.
Helmut Bischof, et al.,
bioRxiv - Cancer Biology 2023
Quote:
Glucose uptake was assessed using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2- NBDG) (Biomol GmbH). Therefore ...
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No products found
because this supplier's products are not listed.
Hannah B. Musgrove, et al.,
bioRxiv - Bioengineering 2022
Quote:
... after which 4 μL of 1 mg/mL 7-AAD (AAT Bioquest, Sunnyvale, CA, USA) was added ...
-
No products found
because this supplier's products are not listed.
Aimee D. Potter, et al.,
bioRxiv - Microbiology 2022
Quote:
... and diluted to ~5*10^7 CFU/ml in 6 ml of media in 15 ml conical tubes (Sarstedt). The bacterial suspension was incubated with rotation at 37°C ...
-
No products found
because this supplier's products are not listed.
Shogo Tsujikawa, et al.,
bioRxiv - Neuroscience 2022
Quote:
... IL-6 (RayBiotech, ELM-IL6-1), PGE2 (Cayman Chemical ...
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No products found
because this supplier's products are not listed.
Karen. M. Marshall, et al.,
bioRxiv - Bioengineering 2023
Quote:
... The dry Laponite coated or uncoated scaffolds were individually submerged in 1 mL of BMP-2 solution of 5 µg/mL or 1 µg/mL (1.5 mg/mL BMP-2 diluted in PBS) in 1.5 mL low-protein binding eppendorf tubes for 24 hours at RT and BMP-2 solution (5 µg/mL and 1 µg/mL ...
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No products found
because this supplier's products are not listed.
Anja Kopp, et al.,
bioRxiv - Biochemistry 2023
Quote:
Screening of crystallization conditions for wild type GSDMD and GSDMDΔ184-194/Δ247-272 in complex with nanobodies VHHGSDMD-1 to VHHGSDMD-6 and the combination of VHHGSDMD-2 plus VHHGSDMD-6 was performed using commercial kits from Molecular Dimensions (Maumee, OH, USA) and Jena Bioscience (Jena ...
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No products found
because this supplier's products are not listed.
Camilla Schinner, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The following primary antibodies were incubated in antibody buffer (Intercept blocking buffer diluted 1:1 in TBS containing 0.2 % tween 20) at 4 °C overnight: Mouse anti-DSG1/2 (61002, Progen, Heidelberg, Germany), mouse anti-DSP (61003 ...
-
No products found
because this supplier's products are not listed.
Yohan S.S. Auguste, et al.,
bioRxiv - Neuroscience 2022
Quote:
... subcutaneous] before being anesthetized using isoflurane (SomnoSuite, Kent Scientific; 3-5% induction, 1-2% maintenance). Once anesthetic depth was achieved ...
-
No products found
because this supplier's products are not listed.
Timothy N. Hoang, et al.,
bioRxiv - Immunology 2020
Quote:
... The sections were incubated with Thyroid Transcription Factor-1 (Clone 8G7G3/1) for overnight at 4°C followed by a detection polymer system (MACH 2™; Biocare Medical). Labeled antibody was visualized by development of the chromogen (DAB Chromogen Kits ...
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No products found
because this supplier's products are not listed.
Drew T. Dunham, et al.,
bioRxiv - Microbiology 2022
Quote:
... 5 μg of RNA was ran on a 8% polyacrylamide urea gel (National Diagnostics SequaGel 1:4) in 1x TBE buffer (100mM Tris base ...
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No products found
because this supplier's products are not listed.
Maximilian W. G. Schneider, et al.,
bioRxiv - Cell Biology 2021
Quote:
... was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 μl of 5 mg ml−1 Biotin-XX-labeled tubulin (Pursolutions ...
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No products found
because this supplier's products are not listed.
Nayab Fatima, et al.,
bioRxiv - Neuroscience 2020
Quote:
Primary human astrocytes (passage 2-7) obtained from ScienCell were cultured in astrocyte medium (ScienCell ...
-
No products found
because this supplier's products are not listed.
Jessica L. Riesterer, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... the samples described above can also be screened via traditional SEM imaging by mounting a semi-thin 250-350 nm section of the 1 mm2 block faces onto a glow discharged 5×7 mm silicon chip (Ted Pella, Inc., cat# 16007). For the silicon chip surface activation ...
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No products found
because this supplier's products are not listed.
Urszula Kozłowska, et al.,
bioRxiv - Cell Biology 2021
Quote:
... (TLR4, Bioss, 1:200, overnight, +4□C), (CD11b ...
-
No products found
because this supplier's products are not listed.
Yinan Hu, et al.,
bioRxiv - Developmental Biology 2019
Quote:
The fluorescent mitochondrial stain 4-(4-Diethylaminostyryl)-1-methylpyridinium iodide (DiASP, AdipoGen) was used to visualize neuromasts (Collazo et al. ...
-
No products found
because this supplier's products are not listed.
Jun Sun, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Rabbit anti-5-HT 1:500 (Immunostar); Rabbit Anti-DCP-1 1:250 (Cell Signalling) ...
-
No products found
because this supplier's products are not listed.
Francesca Querques, et al.,
bioRxiv - Cell Biology 2021
Quote:
... human TCEAL4 transcript variant 1) and pCMV6-TCEAL4 isoform-2 (CAT#: SC335597, NM_001300901, human TCEAL4 transcript variant 5) were both from Origene. TCEAL4 isoform-1 was cloned from pCMV6-TCEAL4-MYC-FLAG with the Gateway cloning system into pDONR223 and then into the destination vector pEGFP_GW ...
-
No products found
because this supplier's products are not listed.
James P Bridges, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 5 % CO2 with 20 µl Calcium 6 loading buffer (Molecular Devices, Cat #R8191): 1x HBSS (Gibco ...
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Isabel R.K. Kuebler, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... The MCH receptor antagonist 6-(4-Chloro-phenyl)-3-[3-methoxy-4-(2-pyrrolidin-1-yl-ethoxy)-phenyl]-3H-thieno[3,2-d]pyrimidin-4-one (GW803430) (Axon Medchem, Groningen, Netherlands) was freshly prepared the day of the treatment ...
-
No products found
because this supplier's products are not listed.
Philipp Gaugler, et al.,
bioRxiv - Plant Biology 2022
Quote:
... They were then diluted 1:200 in 2 mL fresh medium supplemented with 6 μCi mL−1 [3H]-myo-inositol (30–80 Ci mmol−1; Biotrend; ART-0261-5) and grown overnight at 28°C in a spinning wheel ...
-
No products found
because this supplier's products are not listed.
Stephen J. DeCamp, et al.,
bioRxiv - Biophysics 2020
Quote:
Polymerized polyacrylamide gels were first treated with 440 μL of a 1:50 (v/v) mixture of Sulfosuccinimidyl 6-(4'-azido-2'-nitrophenylamino)hexanoate (Sulfo-SANPAH) (ProteoChem) and 50 mM HEPES solution under a UV light for 10 minutes ...
-
No products found
because this supplier's products are not listed.
Susanna Sauret-Güeto, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... and 5 Channel Spectral Scanhead (4 hybrid detectors and 1 PMT). For slides ...
-
No products found
because this supplier's products are not listed.
Eneritz Rueda-Alaña, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... 6-FAM azide (1:500; Lumiprobe) and Sulfo-Cyanine 5 Azide (1:500 ...
-
No products found
because this supplier's products are not listed.
Matthew D. Kerr, et al.,
bioRxiv - Bioengineering 2022
Quote:
... (4-(1,2,4,5-tetrzain-3-yl)phenyl)methanamine (tetrazine amine) was purchased from Kerafast (FCC659, lot: 2014). 1-bicyclo[2.2.1]hept-5-en-2-ylmethanamine (norbornene amine ...
-
No products found
because this supplier's products are not listed.
Anne Bourdais, Benoit Dehapiot, Guillaume Halet,
bioRxiv - Cell Biology 2021
Quote:
... then individually transferred into small (5 µl) drops of fixative (5:1:4 of methanol:acetic acid:water) on a glass-bottom dish (Mattek). After the zona pellucida had gradually dissolved ...
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No products found
because this supplier's products are not listed.
Seiya Yamada, et al.,
bioRxiv - Neuroscience 2021
Quote:
... were blocked for 2 h at 4°C with 5% normal goat serum in PBST and incubated with anti-EGFP (1:2000, Aves Labs, GFP-1010), anti-HA (1:500 ...
-
No products found
because this supplier's products are not listed.
Cherrelle Dacon, et al.,
bioRxiv - Immunology 2022
Quote:
... 7 μm streptavidin beads (Spherotech, SVP-60-5) coated in tandem with 10 μg/mL MERS-CoV spike and 10 μg/mL OC43-CoV spike were re-suspended in a cocktail of 2.5 μg/mL goat anti-human IgG-Alexa Fluor 647 (Jackson ImmunoResearch ...
-
No products found
because this supplier's products are not listed.
Nicole M Sodir, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... rat monoclonal anti-neutrophils (Clone 7/4, Cedarlane, CL8993AP), rat monoclonal F4/80 (clone Cl:A3-1 ...
-
No products found
because this supplier's products are not listed.
Mickaële Hémono, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... using 2 μl of the diluted cDNAs (1:5) and Takyon Blue Master Mix (Eurogentec). All primers used are listed in Table S9 ...
-
No products found
because this supplier's products are not listed.
Sudha Silwal Gautam, et al.,
bioRxiv - Bioengineering 2020
Quote:
... pax 7 (1:1000, rabbit; Lifespan Biosciences, Inc., Seattle, WA, USA), S100 (1:100 ...
-
No products found
because this supplier's products are not listed.
Surya Prakash Rao Batta, et al.,
bioRxiv - Cell Biology 2022
Quote:
HUVECs (passage 2 to 6; PromoCell) were routinely cultured in EBM media supplemented with the provided growth factors kit (Promocell) ...
-
No products found
because this supplier's products are not listed.
Patrick Günther, et al.,
bioRxiv - Immunology 2019
Quote:
Whole blood or buffy coat was diluted in room temperature PBS (1:2 or 1:5, respectively) and layered onto polysuccrose solution (Pancoll; PAN Biotech, Germany) for the enrichment of mononuclear cells by density gradient centrifugation according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Ming Zhou, et al.,
bioRxiv - Plant Biology 2021
Quote:
... (2) A ∼5 cm (15 µL capacity) glass capillary tube (Drummond Scientific, Cat# 1-1000-0150) was inserted in the 2 mm perforation such that the bottom of the tube reaches the 500 µl mark when the lid is refastened to the Eppendorf tube ...
-
No products found
because this supplier's products are not listed.
Rowyn C Liebau, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Membranes were then blocked for 1-2 hours in 5% powdered milk (Amresco, M203-10G-10PK) in PBST ...
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6 micro-well glass bottom plate with #1 cover glass(0.13-0.16mm), micro-well size 14mm, with...
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Laura R Lee, et al.,
bioRxiv - Plant Biology 2023
Quote:
... 5 mL of 1/2 MS with 2% low melt agarose was cast into imaging cuvettes (CellVis product number #C1-1.5H-N) after being filtered through a 0.45 micron nylon filter to remove any particulates that might disturb the path of the light sheet to prepare media “blankets” ...
-
No products found
because this supplier's products are not listed.
Elizabeth B. Draganova, et al.,
bioRxiv - Biophysics 2023
Quote:
... pH 6) and 1 μL of Silver Bullets (Hampton Research) reagent [G3 (0.25% 2,2’-Thiodiglycolic acid ...
-
No products found
because this supplier's products are not listed.
Cambrian Y. Liu, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... 2 μM Cy3 azide (Click Chemistry Tools AZ119-1), and 100 mM ascorbic acid freshly prepared and diluted in Gomori buffer (pH 7.4) ...
-
No products found
because this supplier's products are not listed.
W. Grant Ludlam, et al.,
bioRxiv - Biochemistry 2019
Quote:
The BBS2-7-9 complex was purified at 4°C by affinity purification using 4 mL of Strep-Tactin resin (IBA Life Sciences) packed in a 2 cm diameter column and equilibrated with equilibration buffer (20 mM HEPES pH 7.5 and 20 mM NaCl) ...
-
No products found
because this supplier's products are not listed.
Hui Zhang, et al.,
bioRxiv - Microbiology 2021
Quote:
... HEK293 cells were transiently co-transfected at a ratio of 1:2 (H:L) with PEI (49553-93-7, Polyscience) according to the manufacturer’s instruction ...
-
No products found
because this supplier's products are not listed.
Méghane Sittewelle, Stephen J. Royle,
bioRxiv - Cell Biology 2023
Quote:
... 6 mM of 2-deoxyglucose (Apexbio) and 10 mM of sodium azide (G-Biosciences ...
-
No products found
because this supplier's products are not listed.
Kaiyi Jiang, et al.,
bioRxiv - Genomics 2022
Quote:
... Hepa 1-6 cells (American Type Culture Collection (ATCC) -CRL-1830) ...