-
No products found
because this supplier's products are not listed.
Simon Schäper, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5-bromo-4-chloro-3-indolyl β-d-galactopyranoside (X-Gal, Apollo Scientific) was used at 100 μg/ml ...
-
No products found
because this supplier's products are not listed.
Subash Godar, et al.,
bioRxiv - Biophysics 2021
Quote:
... We detected the bound alkaline phosphatase labeled antibodies by incubating the membrane in BCIP/NBT (5-bromo, 4-chloro, 3- indolylphosphate/nitro-blue tetrazolium, AMRESCO, LLC.) substrate for 30–45 minutes ...
-
No products found
because this supplier's products are not listed.
Tulsi Upadhyay, Vaibhav V Karekar, Ishu Saraogi,
bioRxiv - Biochemistry 2020
Quote:
... All GrpE variants were labeled with DACM (N-(7-dimethylamino-4-methylcoumarin-3-yl)maleimide) (AnaSpec) and DnaK was labeled with BODIPY-fluorescein-N-(2-aminoethyl)-maleimide (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Benjamin Craske, et al.,
bioRxiv - Biochemistry 2022
Quote:
... microtubule seeds containing 7% rhodamine-tubulin (Cytoskeleton Inc., TL590M-B,) were polymerised in BRB80 (80 mM PIPES pH 6.9 ...
-
No products found
because this supplier's products are not listed.
Indu Raghavan, Zhen Q. Wang,
bioRxiv - Bioengineering 2023
Quote:
... 0.97 – 125 mg (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP) (Echelon Biosciences, Inc., Salt Lake City, UT, USA) and 0.97 – 1000 mg/L dimethylallyl pyrophosphate (DMAPP ...
-
No products found
because this supplier's products are not listed.
Cherrelle Dacon, et al.,
bioRxiv - Immunology 2022
Quote:
... 7 μm streptavidin beads (Spherotech, SVP-60-5) coated in tandem with 10 μg/mL MERS-CoV spike and 10 μg/mL OC43-CoV spike were re-suspended in a cocktail of 2.5 μg/mL goat anti-human IgG-Alexa Fluor 647 (Jackson ImmunoResearch ...
-
No products found
because this supplier's products are not listed.
Rosalba Perrone, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... in 3-5% Bovine Serum Albumin - Fraction V in-1X TBS for one hour (Rockland, Baltimore, MD, BSA-1000). Block was removed and slides were incubated in the respective primary antibody diluted in protein block solution for 1 hour or overnight in a humidified chamber ...
-
No products found
because this supplier's products are not listed.
Cynthia M. Arokiaraj, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Cyanine 3 and Cyanine 5 reagents from Akoya Biosciences were used for probe visualization.
-
No products found
because this supplier's products are not listed.
Erica A. Birkholz, et al.,
bioRxiv - Microbiology 2021
Quote:
... 4-7 µl of cells were deposited on R2/1 Cu 200 grids (Quantifoil) that had been glow-discharged for 1 min at 0.19 mbar and 20 mA in a PELCO easiGlow device shortly before use ...
-
No products found
because this supplier's products are not listed.
Ahmet C. Berkyurek, et al.,
bioRxiv - Genetics 2020
Quote:
Western blotting of RPB-9 in the rpb-9 (mj261) and rpb-9 rescue (mjSi70) (Figure S1A) was performed using a human anti-RPB-9 (NBP1-92344, Novus Biologicals) antibody and a mouse anti-tubulin (clone DM1A ...
-
No products found
because this supplier's products are not listed.
Erin M. Euliano, et al.,
bioRxiv - Bioengineering 2024
Quote:
... 2 equivalents of 4-((6-Amino-2-(2-methoxyethoxy)-8-oxo-7,8-dihydro-9H-purin-9-yl)methyl)benzoic acid (Ambeed, Arlington Heights, IL), also known as 1V209 ...
-
No products found
because this supplier's products are not listed.
Md Gulam Musawwir Khan, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Cell survival was measured using the WST-8 (water-soluble Tetrazolium-8: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)- 5- (2,4-disulfophenyl)-2H-tetrazolium) assay kit (CCK-8; Dojindo Molecular Technologies, #CK04) following manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Andrew Barszczyk, et al.,
bioRxiv - Cell Biology 2023
Quote:
... alkaline phosphatase conjugated antibodies were detected colourometrically using BCIP/NBT (nitro-blue tetrazolium and 5- bromo-4-chloro-3’-indolyphosphate) Substrate Solution (#BE116.100ML; Bio Basic Canada Inc). For analysis of bands ...
-
No products found
because this supplier's products are not listed.
W. Grant Ludlam, et al.,
bioRxiv - Biochemistry 2019
Quote:
The BBS2-7-9 complex was purified at 4°C by affinity purification using 4 mL of Strep-Tactin resin (IBA Life Sciences) packed in a 2 cm diameter column and equilibrated with equilibration buffer (20 mM HEPES pH 7.5 and 20 mM NaCl) ...
-
No products found
because this supplier's products are not listed.
Filippo Macchi, Eric Edsinger, Kirsten C. Sadler,
bioRxiv - Genomics 2022
Quote:
... or anti-5-methyl-cytosine (5mC – Aviva Biosystem clone 33D3 ...
-
Glucocorticoid modulator
Sold for research purposes only.
Cat# 1176.0, SKU# 1176-10 mg,
Inquire
Ask
Isabel R.K. Kuebler, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... The MCH receptor antagonist 6-(4-Chloro-phenyl)-3-[3-methoxy-4-(2-pyrrolidin-1-yl-ethoxy)-phenyl]-3H-thieno[3,2-d]pyrimidin-4-one (GW803430) (Axon Medchem, Groningen, Netherlands) was freshly prepared the day of the treatment ...
-
No products found
because this supplier's products are not listed.
Tomohito Higashi, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 2.9 mM KCl, 5 mM HEPES, pH 7.2 (Westerfield, 2007) and mounted in 4-chamber glass bottom dishes (MatTek) for imaging different conditions in parallel (EGTA addition vs ...
-
No products found
because this supplier's products are not listed.
Ayeong So, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Denatured protein extracts were resolved using 4-9% SDS-PAGE (4–15% Mini-PROTEAN® TGX™ Precast Protein Gelscat# 4561083 ...
-
No products found
because this supplier's products are not listed.
Paula Bracco, et al.,
bioRxiv - Biochemistry 2020
Quote:
... 3-deoxydehydroepiandrostendione (10) and 5α-androstan-3-one (11) were purchased from Steraloids Inc ...
-
No products found
because this supplier's products are not listed.
Jakob Schnabl, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Lysate was cleared by centrifugation and incubated for 2h at 4°C with magnetic GFP-Trap agarose (Chromotek). The beads were washed 3 x 10 min with Buffer 3 and were either used for mass spectrometry analysis or the proteins were eluted in 1× SDS buffer with 5 min incubation at 95°C for western blotting ...
-
No products found
because this supplier's products are not listed.
Rinaldo D. D’Souza, et al.,
bioRxiv - Neuroscience 2020
Quote:
... into one of ten cortical areas and performing iontophoretic injections (3 µA, 7 s on/off cycle for 7 minutes; Midgard current source; Stoelting) of biotinylated dextran amine (BDA ...
-
No products found
because this supplier's products are not listed.
Meike E. van der Heijden, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The recording chamber and headplate were attached to the skull using a combination of C and B Metabond Adhesive Luting Cement (Parkell) and methyl methacrylate dental cement (A-M Systems). Mice were allowed to recover from the surgery for at least three days before we began conducting the in vivo electrophysiological recordings.
-
No products found
because this supplier's products are not listed.
Wouter J. Venema, et al.,
bioRxiv - Genomics 2023
Quote:
... and 500 µg cell lysate was incubated for 2h at 4°C with Protein-G-sepharose beads (BV-6511, BioVision) and anti-ERAP2 (AF3830 ...
-
No products found
because this supplier's products are not listed.
David I. Duron, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and Grp94 (MCP230394-CG12-3-B) were obtained from Genecopoeia (Rockville, MD). The DNA was amplified using standard molecular biology approaches ...
-
No products found
because this supplier's products are not listed.
Ami Vadgama, et al.,
bioRxiv - Immunology 2023
Quote:
... 3-100μM protease-activated receptor 4 agonist (PAR-4; Cambridge Biosciences); 0.03-3μM collagen-related peptide (CRP-XL ...
-
No products found
because this supplier's products are not listed.
Sara Đaković, et al.,
bioRxiv - Microbiology 2023
Quote:
... brucei cultured at 37°C and 5% CO2 in HMI-9 media (PAN Biotech) supplemented with 10% fetal calf serum (Gibco) ...
-
No products found
because this supplier's products are not listed.
Reza Nejat, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... Ubiquitin−7-amino-4-methylcourmarin (Ub-AMC) was purchased from Boston Biochem; human ISG15−7-amino-4-methylcourmarin (ISG15-AMC ...
-
No products found
because this supplier's products are not listed.
Alexey V Sarapulov, et al.,
bioRxiv - Immunology 2020
Quote:
... cells were stained with 4 μg/mL 7-AAD (ABD-17501, Biomol) for live/dead cell discrimination and samples were acquired on BD LSR II equipped with 3 laser lines (405 nm ...
-
No products found
because this supplier's products are not listed.
Shahzad S. Khan, et al.,
bioRxiv - Cell Biology 2021
Quote:
... one group (4 mice) received modified rodent diet (Research Diets D01060501) containing MLi-2 and formulated by Research Diets to provide a concentration of 60 mg/kg per day on the basis of an average food intake of 5 g/day for 14 days ...
-
No products found
because this supplier's products are not listed.
Matt J. Matrongolo, et al.,
bioRxiv - Neuroscience 2023
Quote:
... GS-Lectin IB4 488 and 649 (1:100, Vector Biolabs, #B-1205-.5 and #DL-1208-.5), chicken anti-IBA1 (1:500 ...
-
No products found
because this supplier's products are not listed.
Camille Mazo, et al.,
bioRxiv - Neuroscience 2022
Quote:
... An array of 4 tungsten electrodes (∼3 MΩ; FHC) was glued together and was slowly lowered into the OB ...
-
No products found
because this supplier's products are not listed.
Thorsten M. Leucker, et al.,
bioRxiv - Cell Biology 2021
Quote:
... PCSK9 mRNA expression was assessed by real-time PCR using PCSK9 primer sets 5’-TGTCTTTGCCCAGAGCATC-3’ and 5’-GTCACTCTGTATGCTGGTGTC-3 (Integrated DNA Technologies) and SYBR Select Master Mix (ThermoFisher, ABI 4472908).
-
No products found
because this supplier's products are not listed.
Eirini Tsekitsidou, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 3 μg/mL blasticydin and 200 μg/mL hygromycin-B (Research Products International, H75000-1). MiniTurbo expression was induced with addition of 1 μg/mL doxycycline (Sigma-Aldrich ...
-
No products found
Mohummad Aminur Rahman, et al.,
bioRxiv - Cancer Biology 2020
Quote:
Human ATG7 shRNA-GFP (5’-CAGTGGATCTAAATCTCAAACTGAT-3’) and scrambled control shRNA-GFP (5’-GGGTGAACTCACGTCAGAA −3’) lentiviral plasmids were purchased from Applied Biological Materials Inc ...
-
No products found
because this supplier's products are not listed.
Ana S Almeida, et al.,
bioRxiv - Microbiology 2021
Quote:
... and three 3–4 mm sterile glass beads (Biospec Products). Next ...
-
No products found
because this supplier's products are not listed.
Alexia Caillier, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The supernatant was diluted with 9 mL cold PBS and free puromycin was removed by centrifugation using Macrosep 3 kDa centrifugal filters (Pall). Concentrated lysate was diluted in non-denaturing lysis buffer (10 mM Tris pH 7.4 ...
-
No products found
because this supplier's products are not listed.
He-Chin Hsieh, et al.,
bioRxiv - Microbiology 2023
Quote:
... and BA.4/5 (Genetex, Cat. No. GTX137098-pro) were mixed with RBD-LTA ...
-
No products found
because this supplier's products are not listed.
Victoria A. Bonnell, et al.,
bioRxiv - Genomics 2023
Quote:
... Sonicate the chromatin until sufficiently sheared (130µL, 5% duty cycle, 75W peak incident power, 200 cycles per burst, 7°C, for 5 minutes using Covaris Focus-Ultrasonicator M220). The immunoprecipitation step included ...
-
LC Laboratories' Product Number D-7878 - Daidzin (4',7-Dihydroxyisoflavone...
Cat# D-7878, SKU# D-7878_300mg,
300 mg, $144.00
Ask
Jun-yi Zhu, et al.,
bioRxiv - Genetics 2020
Quote:
Rapamycin (LC Laboratories, 53123-88-9) was dissolved in ethanol and added to standard fly food at appropriate concentrations (0.1 and 200 μM) ...
-
No products found
because this supplier's products are not listed.
Yoann G. Santin, et al.,
bioRxiv - Microbiology 2023
Quote:
... manually back blotted for 3-4 secondes and flash-frozen in liquid ethane using a CP-3 plunger (Gatan). Data were collected on a 300-kV CRYO ARM™ 300 (JEM-Z300FSC ...
-
No products found
because this supplier's products are not listed.
Shuntaro Morikawa, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Fluorescence for cell viability and luminescence for caspase-3/7 activity was measured using Infinite M1000 plate reader (Tecan). Caspase-3/7 activity was normalized to cell viability according to the manufacturer’s protocol.
-
No products found
because this supplier's products are not listed.
Yun-Seok Choi, et al.,
bioRxiv - Biochemistry 2019
Quote:
... cells were incubated overnight at 4 °C with anti-HA antibody (Sigma-Aldrich clone HA-7 or Bethyl Laboratories A190-108A ...
-
No products found
because this supplier's products are not listed.
Yen-Der Li, et al.,
bioRxiv - Biochemistry 2023
Quote:
... transfected with 9 μg HA-tagged and 9 μg Flag-tagged constructs using TransIT-LT1 transfection reagents (Mirus). The transfected cells were cultured for another two days ...
-
No products found
because this supplier's products are not listed.
Bradley M. Roberts, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Iris 9 Scientific CMOS camera (Teledyne Photometrics), 525/50 nm emission filter (Cairn Research) ...
-
No products found
because this supplier's products are not listed.
Nisa Cuevas, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Virus was injected bilaterally in the DMS or DLS at 4.6 nL/5 seconds (9 minutes) using a borosilicate pipette (Drummond scientific, >25 µm) coupled to a nanoinjector (Nanoject II Drummond Scientific) ...
-
No products found
because this supplier's products are not listed.
Aimee D. Potter, et al.,
bioRxiv - Microbiology 2022
Quote:
... and diluted to ~5*10^7 CFU/ml in 6 ml of media in 15 ml conical tubes (Sarstedt). The bacterial suspension was incubated with rotation at 37°C ...
-
No products found
because this supplier's products are not listed.
Ron Refaeli, et al.,
bioRxiv - Neuroscience 2022
Quote:
... the relevant brain slices were incubated for 7 days at 4°C with rabbit anticFos primary antibody (Synaptic system, #226003), and for the mCherry staining ...
-
No products found
because this supplier's products are not listed.
Jean-Philippe Leduc-Gaudet, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The peritoneum was closed in two separate layers using 3-0 absorbable polyfilament interrupted sutures and the skin was closed with a 9 mm surgical staple (AutoClip® System, Fine Science Tools, Foster City, CA). All animals received subcutaneous injections of Buprenex® (0.05 to 0.2 mg/kg buprenorphine in 1 ml of 0.9% saline ...
-
No products found
because this supplier's products are not listed.
Madeleine Linneberg-Agerholm, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... and FCS Express 7 (De Novo Software).
-
No products found
because this supplier's products are not listed.
Elyse M. Digby, et al.,
bioRxiv - Biophysics 2021
Quote:
MRC-9 (human lung fibroblasts) were purchased from American Type Culture Collection (ATCC) and cultured in Eagle’s Minimum Essential Medium (EMEM ...