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No products found
because this supplier's products are not listed.
Yun Deng, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... with 5-bromo-4-chloro-3-indolyl-phosphate nitro blue tetrazolium staining (Vector Laboratories, Burlingame, CA, USA). 10 ∼ 30 embryos were used for each probe ...
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No products found
because this supplier's products are not listed.
Ed Zandro M. Taroc, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... Cell counts were done on serial sections immunostained for GnRH-1 at E13.5 (n=3) and E15.0 (n=4) and visualized under bright field (immunoperoxidase) or epi-fluorescence illumination (20×; Leica DM4000 B LED), according to their anatomical location [i.e. ...
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No products found
because this supplier's products are not listed.
Janelle Lauer, et al.,
bioRxiv - Biophysics 2019
Quote:
... 2’-(3’)-bis-O-(N-methylanthraniloyl)-GDP (Jena Biosciences). Rab5 was loaded with mant-GDP in Rab5 buffer + 10mM EDTA and 5-fold excess mant-GDP ...
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No products found
because this supplier's products are not listed.
Julia Martz, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and placenta (n = 7; Enzo Life Sciences, Farmingdale, NY), maternal plasma cytokines (IL-6 and IL-17A ...
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No products found
because this supplier's products are not listed.
Julia Franzen, et al.,
bioRxiv - Genomics 2020
Quote:
... Human umbilical vein endothelial cells were isolated from umbilical cords of healthy donors after cesarean sections (HUVECS; n = 4; RWTH Aachen) (55) or obtained from Lonza (n = 3, Basel, Switzerland) (8) ...
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No products found
because this supplier's products are not listed.
A.R. von Gundlach, et al.,
bioRxiv - Biophysics 2019
Quote:
... after in situ activation with four equivalents of N,N,N′,N′-Tetramethyl-O-(1H-benzotriazol-1-yl)uronium hexafluorophosphate (HBTU; Carbosynth, Berkshire, United Kingdom) and eight equivalents of N-Methylmorpholine (NMM ...
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No products found
because this supplier's products are not listed.
Mei Dang, Tongyang Li, Jianxing Song,
bioRxiv - Biophysics 2022
Quote:
The formation of liquid droplets was imaged on 50 μl of the full-length N protein or N (1-249) samples by DIC microscopy (OLYMPUS IX73 Inverted Microscope System with OLYMPUS DP74 Color Camera ...
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No products found
because this supplier's products are not listed.
Zhaoduan Liang, et al.,
bioRxiv - Immunology 2019
Quote:
... 5-Bromo-4-chloro-3-indolyl phosphate/Nitro blue tetrazolium (BCIP/NBT, Mabtech) was used to develop the immune-spot according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Nejla Ozirmak Lermi, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... genomic DNA and RNA were extracted from fresh frozen tumor (n=2) and normal (n=3) samples using the ZR-Duet DNA/RNA MiniPrep extraction kit (ZYMO RESEARCH, Irvine, CA). Quantification was performed with a NanoDrop One™ spectrophotometer (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Esther Wehrle, et al.,
bioRxiv - Bioengineering 2021
Quote:
... n=8) or collagen scaffolds+BMP-2 (2.5μg/scaffold; PeproTech, London, UK; n=8). Perioperative analgesia (25 mg/L ...
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No products found
because this supplier's products are not listed.
Samantha C. Lauby, Patrick O. McGowan,
bioRxiv - Neuroscience 2020
Quote:
... was measured in the pup serum (n = 5-7 per group) using an ELISA (MP Biomedicals Inc., USA) following the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Francina Mesquida-Veny, et al.,
bioRxiv - Neuroscience 2022
Quote:
... followed by 3 O/N of primary antibody incubation (SCG-10 (1:1000, Novus Biologicals) in 2% BSA ...
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No products found
because this supplier's products are not listed.
Dina Rekler, Chaya Kalcheim,
bioRxiv - Developmental Biology 2022
Quote:
... rabbit anti-RFP (1:1000, OriGene, AP09229PU-N), guinea pig anti-pSmad1/5/8 (1:300 ...
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No products found
because this supplier's products are not listed.
Tulsi Upadhyay, Vaibhav V Karekar, Ishu Saraogi,
bioRxiv - Biochemistry 2020
Quote:
... All GrpE variants were labeled with DACM (N-(7-dimethylamino-4-methylcoumarin-3-yl)maleimide) (AnaSpec) and DnaK was labeled with BODIPY-fluorescein-N-(2-aminoethyl)-maleimide (Invitrogen ...
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No products found
because this supplier's products are not listed.
Denis Antonets, et al.,
bioRxiv - Cell Biology 2019
Quote:
... TagRFP fragment without start codon was obtained by PCR with TagRFP-woATG-F 5’-GT CGGTACCGTGTCTAAGGGCGAAGAGCTG-3’ n BFPX3-R 5’-GCGCTTAAGTTAATTAAGCTTGTGCCCCA-3’ primers and pTagRFP-N vector (Evrogen) as a DNA source ...
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No products found
because this supplier's products are not listed.
Vivian K. Rojas, et al.,
bioRxiv - Microbiology 2024
Quote:
... The chromogenic molecule X-Phos (5-bromo-4-chloro-3-indolyl phosphate, Chem-Impex) was added to agar plates with the purpose of detecting S ...
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No products found
because this supplier's products are not listed.
Connor C. Leek, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... Two histological sections per sample treated with EdU (WT n=4; Fgf9null n=3) were imaged using a Zeiss Axio-observer Z1 microscope (Axio.Observer. Z1, Carl Zeiss, Gottingen, Germany). Differential interference contrast (DIC ...
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No products found
because this supplier's products are not listed.
Samantha L Scudder, et al.,
bioRxiv - Neuroscience 2019
Quote:
... N-succinyl-Leu-Leu-Val-Tyr-7-amino-4-methylcoumarin (Suc-LLVY-AMC) substrate was used (BACHEM). GST-Ubl and His-UIM constructs were a gift from the Alfred Goldberg lab ...
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No products found
because this supplier's products are not listed.
Emily J. Talbot, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 4 µM digitonin) with or without 7 µM 2’,3’-cGAMP (Invivogen). Cells were further washed in PBS and incubated in culture medium until collection ...
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No products found
because this supplier's products are not listed.
Olivier Binda, et al.,
bioRxiv - Biochemistry 2022
Quote:
The installation of a monomethyl-lysine analog at the mutated cysteine of the H3KC79 (C110A) histone was done as described (52) using the 2-chloro-N-methylethanamine hydrochloride (Toronto Research Chemicals C428323) to generate H3KC79me1 (C110A) ...
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No products found
because this supplier's products are not listed.
Moriya Slavin, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Expression plasmids (Nsp1/Nsp2/N) and PEI (Polysciences, 260008-5) were separately diluted in Opti-MEM1 (Gibco 31985-047 ...
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No products found
because this supplier's products are not listed.
Vo Hoa Thi My, et al.,
bioRxiv - Immunology 2021
Quote:
... M and N protein pools [1 µg/mL] (PepTivator® SARS-CoV-2 regents; Miltenyi Biotec) in 96-well U-bottom plates at 0,5-1×106 PBMCs per well ...
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No products found
because this supplier's products are not listed.
L Simões de Oliveira, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and incubated for an hour at room temperature with secondary antibodies (IRDye 800CW Goat anti Rabbit IgG- 1:10,000, #P/N 925-32211; IRDye 680LT Goat anti Mouse IgG- 1:10,000, #P/N 925-68020, LI-COR Biotechnology). Membranes were washed with TBST1X ...
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No products found
because this supplier's products are not listed.
Alison Dumont, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Caspase 3/7 dye (4440, Sartorius, 1/1000) and/or propidium Iodide (PI ...
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No products found
because this supplier's products are not listed.
Rashmi K. Shrestha, et al.,
bioRxiv - Biochemistry 2022
Quote:
... labeled protein was visualized using the AP substrate 5-bromo-4-chloro-indolyl-phosphate/nitro blue tetrazolium (BCIP/NBT; Southern Biotech).
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No products found
because this supplier's products are not listed.
Amanda M. Hamilton, et al.,
bioRxiv - Cell Biology 2019
Quote:
Viability of unlabeled and ferumoxytol-labeled murine MSCs (n=4) and human MSCs (n=3) was assessed by Trypan blue dye exclusion using ViCell (Beckman Coulter).
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No products found
because this supplier's products are not listed.
Yang C. Zeng, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... DNA oligonucleotides /5AmMC6/GGGATAAGTTGATTGCAGAGC-3’(anti-handle) were modified with a 5’ amine and covalently coupled to Cy5 fluorophores via N-hydroxysuccinimide (NHS) ester coupling (Lumiprobe). DNA oligonucleotide (1 mM in ddH2O ...
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No products found
because this supplier's products are not listed.
Charlotte Garot, et al.,
bioRxiv - Bioengineering 2022
Quote:
... and BMP50 (n = 3 3D mini-scaffolds per geometry and per BMP-2 concentration). The homogeneity of BMP-2 loading inside the film-coated 3D mini-scaffolds was assessed using a BMP-2 loading solution containing 5% of BMP-2Rhod and a fluorescence macroscope (Macrofluo Z16 Apo ...
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No products found
because this supplier's products are not listed.
Stephan Kamrad, et al.,
bioRxiv - Genetics 2019
Quote:
... column (New Objective, PF360-75-10-N-5) packed in house with 1.9 um C18 beads (Dr ...
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No products found
because this supplier's products are not listed.
Jia Hui Li, et al.,
bioRxiv - Biophysics 2020
Quote:
... The needle was then glued to a 6×4×2 mm Neodyn magnet (QM-06×04×02-N, Magnets4you) and attached to a motorized micromanipulator (PatchMan, Eppendorf). The magnetic needle was lowered until it touched the bottom of a dummy sample dish and raised again so that it was placed above the bottom of the sample with the very tip in focus around 100 μm above the focus of the glass surface.
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No products found
because this supplier's products are not listed.
Jeffrey W. Pippin, et al.,
bioRxiv - Cell Biology 2021
Quote:
... (2) aged-matched control mice (n=7) that received weekly IP injections of the isotype control IgG2A antibody (2A3, Bio X Cell, Lebanon, NH) at 12 mg/kg for 8 weeks ...
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No products found
because this supplier's products are not listed.
Fabia Febbraro, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... after which 4 ml maturation medium (Cerebral organoid kit, cat. n. 08570, StemCell Technologies) were gently added to each well ...
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No products found
because this supplier's products are not listed.
Siyuan Zhao, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Slices were then incubated in a secondary antibody solution at 4 °C for 5-7 days with protection from light (1:500, Alexa Fluor 647 donkey anti-chicken, Jackson Immunoresearch, USA ...
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No products found
because this supplier's products are not listed.
Subash Godar, et al.,
bioRxiv - Biophysics 2021
Quote:
... We detected the bound alkaline phosphatase labeled antibodies by incubating the membrane in BCIP/NBT (5-bromo, 4-chloro, 3- indolylphosphate/nitro-blue tetrazolium, AMRESCO, LLC.) substrate for 30–45 minutes ...
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No products found
because this supplier's products are not listed.
Janelle S. Weir, et al.,
bioRxiv - Neuroscience 2023
Quote:
... we used in-house developed microfluidic chips interfaced with microelectrode arrays (mMEAs; n=7) and 8-well chambered slides (Ibidi, 80841; n=2). Design and fabrication of the mMEAs was conducted as reported previously by our group (Winter-Hjelm N ...
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No products found
because this supplier's products are not listed.
Aya M. Saleh, et al.,
bioRxiv - Bioengineering 2021
Quote:
... 5 mM tris(3-hydroxypropyltriazolylmethyl)amine (THPTA; Click Chemistry Tools), 2 mM copper sulfate ...
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No products found
because this supplier's products are not listed.
Cecilia Patitucci, et al.,
bioRxiv - Physiology 2023
Quote:
... N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) (HEPES-KOH pH 7.4 20 mM; EuroMedex, 10-110); Sucrose 110mM ...
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No products found
because this supplier's products are not listed.
Shahanshah Khan, et al.,
bioRxiv - Immunology 2021
Quote:
... SARS-CoV-2 N (RayBiotech, 230-30164), SARS-CoV-2 M (MyBioSource ...
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No products found
because this supplier's products are not listed.
Morgan E. Mouchka, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
... Colonies were screened on LB plates with 50 μg/mL ampicillin and 9.5 mg/mL X-gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside; APExBIO) and Sanger sequencing of the J was used to confirm cloning and successful transformation of the ancestral λ sequence.
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Chromatographically purified through step five of the procedure of Yamada and Yasunobu, J. Biol....
Cat# LS003114,
3 ku, $425.00
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Boao Xia, et al.,
bioRxiv - Bioengineering 2020
Quote:
... acellular PCL and GA or GP crosslinked dMEP scaffolds (n = 4/group) were digested in 2 mg/mL collagenase type 2 solution (Worthington) for 24 h at 37°C ...
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No products found
because this supplier's products are not listed.
Alexandra Paul, et al.,
bioRxiv - Cell Biology 2022
Quote:
... littermates were randomly selected to be either kept on a standard laboratory chow diet (CD, Special Diets Services, N=5) or on a high fat diet (HFD, D12492, Research Diets Inc., N=5). Mice were fasted for 4h prior to tissue collection ...
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No products found
because this supplier's products are not listed.
Daisuke Takao, et al.,
bioRxiv - Cell Biology 2019
Quote:
CEP152-N (1:1,000; Bethyl, A302-479A)
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No products found
because this supplier's products are not listed.
Sakthi Rajendran, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... tissues from secondary GBM (Grade III (n=44), Grade IV (n=23)) and LGG (grade II, n=42) using MasterPure kit (Epicentre). Raw reads from the RNA-seq data were processed using an in-house pipeline that uses STAR for read alignment ...
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No products found
because this supplier's products are not listed.
Luke A Johnson, et al.,
bioRxiv - Neuroscience 2020
Quote:
... In all patients a directional “1-3-3-1” electrode was used (4/5 patients: Abbott Infinity model 6172 ...
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No products found
because this supplier's products are not listed.
Michael P. Vincent, et al.,
bioRxiv - Bioengineering 2021
Quote:
... or HUVECs (n=3) were seeded in glass-bottom 96-well plates (Greiner) at a seeding density of 25,000 cells/well ...
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No products found
because this supplier's products are not listed.
Khyati Gohil, et al.,
bioRxiv - Bioengineering 2022
Quote:
... The images of these droplets (n ≥ 3) were acquired with Metamorph software (Molecular Devices) with a 30-s interval and a 100-ms exposure time.
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No products found
because this supplier's products are not listed.
Ming Bi, et al.,
bioRxiv - Systems Biology 2023
Quote:
Permethylated N-glycans were trapped on a C18 (5 μm, 300 Å, Jupiter, Phenomenex) trap column and separated on a C18 analytical column (75 μid ...
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No products found
because this supplier's products are not listed.
Daniel Bsteh, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... with following appropriate antibodies at 4 °C o/n a rotating wheel: H3K27me3 (Diagenode, C15410195), RING1B (Cell Signaling ...
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No products found
because this supplier's products are not listed.
Samantha R. Mackin, et al.,
bioRxiv - Microbiology 2022
Quote:
... halted with 2 N H2SO4 and read at 450 nm using a microplate reader (BioTek). Mean serum endpoint titers were calculated with curve fit analysis of optical density (OD ...
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No products found
because this supplier's products are not listed.
Chunchu Deng, et al.,
bioRxiv - Neuroscience 2021
Quote:
... polyclonal guinea pig Ca2+ channel N-type alpha-1B channel (152305, Synaptic Systems, 1:250) and monoclonal rat anti-mCherry (M11217 ...