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Irina V. Mikheyeva, et al.,
bioRxiv - Microbiology 2023
Quote:
... Cells were stained with 300 µM 3-[[(7-Hydroxy-2-oxo-2H-1-benzopyran-3-yl)carbonyl]amino]-D-alanine hydrocholoride (HADA, MedChemExpress, Cat. #HY-131045/CS-0124027) and perfused with 0.85X PBS prior to the osmotic shock.
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No products found
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Marley J. Dewey, et al.,
bioRxiv - Bioengineering 2021
Quote:
... then decalcified for 4-8 days in CalciClear decalcifying solution (National Diagnostics). Samples were rinsed in several changes of water after decalcification ...
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No products found
because this supplier's products are not listed.
Morgan E. Mouchka, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
... Colonies were screened on LB plates with 50 μg/mL ampicillin and 9.5 mg/mL X-gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside; APExBIO) and Sanger sequencing of the J was used to confirm cloning and successful transformation of the ancestral λ sequence.
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No products found
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Sangin Kim, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 5-chloro-2ʹ-deoxyuridine (CldU) (#105478) (MP Biomedicals); Shield1 (632189 ...
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No products found
because this supplier's products are not listed.
Marco Schade, et al.,
bioRxiv - Paleontology 2022
Quote:
All other specimens (Figs. 4, 5, 7, 8; Supplementary Figs. 5–11) were documented with a Xradia MicroXCT-200 (Carl Zeiss Microscopy GmbH, Jena, Germany) of the Imaging center of the Department of Biology ...
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No products found
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Md Gulam Musawwir Khan, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Cell survival was measured using the WST-8 (water-soluble Tetrazolium-8: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)- 5- (2,4-disulfophenyl)-2H-tetrazolium) assay kit (CCK-8; Dojindo Molecular Technologies, #CK04) following manufacturer’s instructions ...
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No products found
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Xuyong Chen, Lingling Liu, Ruoning Wang,
bioRxiv - Immunology 2022
Quote:
... anti–mIL-4 (8 μg/mL, Bio X Cell), and anti–mIFN-γ (8 μg/mL ...
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No products found
because this supplier's products are not listed.
James Chen, et al.,
bioRxiv - Biophysics 2021
Quote:
... 3-([3-cholamidopropyl]dimethylammonio)-2-hydroxy-1-propanesulfonate (CHAPSO, Anatrace) was added to the sample (8 mM final) ...
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No products found
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Tim Nierhaus, et al.,
bioRxiv - Microbiology 2021
Quote:
... pooled and concentrated to 7-8 mg/ml using Vivaspin 20 concentrators (30 kDa MWCO, Sartorius). Small aliquots were flash-frozen in liquid nitrogen and stored at −80°C ...
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No products found
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P. C. Steketee, et al.,
bioRxiv - Microbiology 2021
Quote:
... UK). Libraries were prepared from 8 samples (4× T. brucei, 4× T. congolense) using the TruSeq Stranded mRNA kit (Illumina) and 2 × 75 bp paired-end sequencing was carried out using a HiSeq 4000 system (Illumina) ...
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No products found
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Zhe Zhang, Jay R. Gibson, Kimberly M. Huber,
bioRxiv - Neuroscience 2021
Quote:
... Whole cell recordings of L2/3 and L5 pyramidal neurons were obtained using borosilicate pipettes (4-7 MΩ) and a Multiclamp 700A amplifier (Molecular Devices). Internal solution contained (in mM) ...
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No products found
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Clara Hozer, Fabien Pifferi,
bioRxiv - Physiology 2021
Quote:
... We assayed plasma 8-hydroxy-2’-deoxyguanosine (8-OHdG) levels (OxiSelect™ Oxidative DNA Damage Elisa kit, Cell Biolabs Inc.) and insuline-like growth-factor 1 (IGF-1 ...
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No products found
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Yun Deng, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... with 5-bromo-4-chloro-3-indolyl-phosphate nitro blue tetrazolium staining (Vector Laboratories, Burlingame, CA, USA). 10 ∼ 30 embryos were used for each probe ...
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No products found
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Matthew Yedutenko, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Patch pipettes (resistance 7-8 MOhm, PG-150T-10; Harvard Apparatus, Holliston, Massachusetts) were pulled with a Brown Flaming Puller (Model P-87 ...
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No products found
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Aditya N. Bade, et al.,
bioRxiv - Bioengineering 2021
Quote:
CEST imaging was performed on controls (n = 8) and 3TC-treated mice (n = 7) on a 7 Tesla MRI scanner (Bruker BioSpec 70/20, Billerica, MA). A Bruker-made volume quadrature RF coil was employed for signal transmission and a Bruker 4-element coil array was used for signal reception ...
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No products found
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Nina Criado Santos, et al.,
bioRxiv - Cell Biology 2022
Quote:
... cells were loaded with 4 μM Fluo-8-AM (AAT Bioquest) for 30 min at 37 °C ...
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No products found
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Trinh Lam, et al.,
bioRxiv - Microbiology 2021
Quote:
... centrifuged at 7000 RPM for 7 min at 4 °C (Eppendorf model 5804R). The supernatant was discarded ...
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No products found
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Andrew H. Cooper, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 4 µL FastBlue (FB; 2% in sterile PBS; CAS# 73819-41-7; Polysciences Inc., Warrington, PA USA) per paw was then slowly injected ...
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No products found
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Yi Jiang, et al.,
bioRxiv - Microbiology 2023
Quote:
... The samples were run on precast SurePAGE gels (Bis–Tris, 10×8, 4%– 12%, 15 wells; GenScript) and transferred to polyvinylidene difluoride membranes (Beyotime Biotechnology ...
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No products found
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Paloma P Maldonado, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Consecutive xyt-stacks were acquired at a frame rate of 4-7 Hz through a 40x (0.8 NA, Olympus) or a 16x (0.8 NA ...
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No products found
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Qing Tang, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... The kinetics of substrate cleavage (increase in fluorescence of the liberated 7-amino-4-methylcoumarin, AMC) was measured using a fluorometer (BioTek, USA) set at 370 nm excitation and 460 nm emission at 37°C ...
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No products found
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Yunlong Cao, et al.,
bioRxiv - Immunology 2022
Quote:
... Tetramethylbenzidine (TMB) (Solarbio, 54827-17-7) was added into each well ...
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No products found
because this supplier's products are not listed.
Wenbo Liu, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... The cells are centrifugated at 600 g for 7 min at 4 °C and resuspended with 180 μl running buffer (Miltenyi Biotec, 130-091-221) for MACS selection.
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No products found
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Rachael Gordon, et al.,
bioRxiv - Immunology 2019
Quote:
... were detected with bromo-4-chloro-3-indolyl phosphate substrate (Southern Biotech).
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No products found
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Zhaoduan Liang, et al.,
bioRxiv - Immunology 2019
Quote:
... 5-Bromo-4-chloro-3-indolyl phosphate/Nitro blue tetrazolium (BCIP/NBT, Mabtech) was used to develop the immune-spot according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Walden Li, Ryan M. Wyllie, Paul A. Jensen,
bioRxiv - Microbiology 2020
Quote:
... Strains carrying the pLacZ plasmid appear blue when plated on 0.008% X-gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Zymo Research, CA, USA). X-gal concentrations above 0.008% appear to inhibit growth of S ...
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No products found
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Julia L. Daiß, et al.,
bioRxiv - Biochemistry 2021
Quote:
The selected cell line RPA1-sfGFP was cultivated adherently and adapted to suspension growth as follows: Cells from 8 flasks (about 7×107 cells total; 83.3912.302, Sarstedt) were detached by incubation with trypsin (25300 ...
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No products found
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Anastasia K. Pickford, et al.,
bioRxiv - Microbiology 2021
Quote:
... 4 µg of chromatin were immunoprecipitated overnight at 4°C with 8 µg of antibody against H3K9me3 (Diagenode, C15410193) previously coupled to protein A/G magnetic beads provided in the kit ...
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No products found
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Nicholas W. Villarino, et al.,
bioRxiv - Neuroscience 2022
Quote:
... ADVASEP-7 (Biotium) was also resuspended at 500 μM in HBSS ...
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No products found
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Yun-Seok Choi, et al.,
bioRxiv - Biochemistry 2019
Quote:
... cells were incubated overnight at 4 °C with anti-HA antibody (Sigma-Aldrich clone HA-7 or Bethyl Laboratories A190-108A ...
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8 Well Chambered Cover Glass with #1 cover glass (0.13-0.16mm), with lid, sterilized. Designed...
Cat# C8-1-N,
48/case, $219.00
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Mai Pho, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Cells were imaged in either 4 well cover glass dishes or 8 well cover glass chambers (Cellvis). For time lapse data ...
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No products found
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E. V. Ely, et al.,
bioRxiv - Bioengineering 2023
Quote:
... or 8-well cell culture slides (CCS-8; Mattek) for 1 day at a density of 4.16e4 cells/cm2 (4.16e5 cells/well ...
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No products found
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Feng Long, et al.,
bioRxiv - Genomics 2020
Quote:
... overnight at 4°C and secondary antibody [Alexa Fluor 594 – conjugated Affinipure Donkey Anti-Rabbit IgG(H+L) antibody (SA0006-8, Proteintech Group)] for 1 h at 37°C in dark ...
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No products found
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Madeleine Linneberg-Agerholm, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... and FCS Express 7 (De Novo Software).
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No products found
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Naba Al-Sari, et al.,
bioRxiv - Biophysics 2020
Quote:
... trioctanoin-1,1,1-13C3 (TG(8:0/8:0/8:0)-13C3) and 1-palmitoyl-2-hydroxy-sn-Glycero-3phosphatidylcholine (LPC(16:0)) from Larodan AB (Solna ...
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The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell...
Cat# B34304, SKU# B34304-25 mL (2500 rxns),
25 mL (2500 rxns), $201.00
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Kristina A.M. Arendt, et al.,
bioRxiv - Cancer Biology 2020
Quote:
In vitro cell proliferation was determined using WST-8 [water soluble tetrazolium-8 or 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulphophenyl)-2H-teterazolium] assay (Bimake; Munich, Germany). For this ...
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No products found
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Matteo Tassinari, et al.,
bioRxiv - Microbiology 2020
Quote:
... 1μL was then spotted on LB media supplemented with 40 μg/mL of 5-bromo-4-chloro-3-indolyl-β-D-galactoside (X-gal) (Euromedex), ampicillin (Amp ...
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No products found
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Le Xiong, et al.,
bioRxiv - Genomics 2021
Quote:
... 1*10^8 cells were labeled with 500 µM 4-thiouridine (4sU, Carbosynth, 13957-31-8) for 5min ...
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No products found
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Erica A. Birkholz, et al.,
bioRxiv - Microbiology 2021
Quote:
... 4-7 µl of cells were deposited on R2/1 Cu 200 grids (Quantifoil) that had been glow-discharged for 1 min at 0.19 mbar and 20 mA in a PELCO easiGlow device shortly before use ...
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No products found
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Yuyang Wang, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... 3,4-Dihydro-2H-pyrano[2,3-b]quinolin-7-yl)-(cis-4-methoxycyclohexyl)-methanone (JNJ 16259685) was purchased from HelloBio (Princeton, NJ, USA). N-[4-Chloro-2-[(1,3-dihydro-1,3-dioxo-2H-isoindol-2-yl)methyl]phenyl]-2-hydroxybenzamide (CPPHA ...
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No products found
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Hannah Walters, Lynne S Cox,
bioRxiv - Cell Biology 2020
Quote:
... Cells were seeded at 4-8×103 cells/cm2 in filter-capped flasks or multi-well plates (Greiner) and incubated with complete media in a humidified incubator at 37oC in 5% CO2 and 20% O2 ...
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No products found
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Alexia Caillier, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The PVDF membranes were incubated overnight at 4◦C in 2% BSA (Research Products International, 9048-46-8) with primary antibody ...
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No products found
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KR Bowles, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Multiplexed immunofluorescent staining was carried out on 4-6µm sections using the Opal Polaris 7 color IHC detection kit (Akoya biosciences) according to manufacturer’s instructions ...
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No products found
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Ron Refaeli, et al.,
bioRxiv - Neuroscience 2022
Quote:
... the relevant brain slices were incubated for 7 days at 4°C with rabbit anticFos primary antibody (Synaptic system, #226003), and for the mCherry staining ...
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No products found
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Giorgia Napoletani, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... and resolved by electrophoresis on a 4-20% or 8-16% polyacrylamide gradient gel (Mini-Protean TGX, Bio-Rad). Proteins were transferred to nitrocellulose or PVDF membranes (Immobilon-P membrane ...
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No products found
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Siyuan Zhao, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Slices were then incubated in a secondary antibody solution at 4 °C for 5-7 days with protection from light (1:500, Alexa Fluor 647 donkey anti-chicken, Jackson Immunoresearch, USA ...
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No products found
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Tomasz Czerniak, James P Saenz,
bioRxiv - Biochemistry 2021
Quote:
... 7-deaza-GTP (Trilink Biotechnologies, USA) was used ...
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Prepared to contain collagenase and caseinase activities approximately four-fold higher than...
Cat# LS005332,
100 mg, $68.00
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Francesco Manfredi, et al.,
bioRxiv - Immunology 2023
Quote:
... and then digested for 30 minutes under gentle agitation at 37°C with 7 mL of 4 mg/mL of type III collagenase (Worthington Biochemical, Lakewood, NJ) and 0.05 mg/mL of DNase I (Sigma ...
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No products found
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Arun S. Rajkumar, John P. Morrissey,
bioRxiv - Synthetic Biology 2020
Quote:
... with a REZEX 8 μL 8% H+ organic acid column (300 ×7.8 mm) (Phenomenex, CA, USA) operating at 65◦C using 0.01 N H2SO4 as a solvent at a flow rate of 0.6 mL min−1 ...
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No products found
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David L. Prole, Colin W. Taylor,
bioRxiv - Cell Biology 2019
Quote:
HeLa and COS-7 cells (American Type Culture Collection) were cultured in Dulbecco’s modified Eagle’s medium/F-12 with GlutaMAX (ThermoFisher ...