-
No products found
because this supplier's products are not listed.
Shalini Gupta, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... The N- and C-peptides were labeled with maleimide-derivatized DY549P1 (Dyomics), DY649P1 (Dyomics) ...
-
No products found
because this supplier's products are not listed.
Christian S. Stevens, et al.,
bioRxiv - Microbiology 2022
Quote:
... and fluorescent probe (5’-6FAM-ACAGACGTTGTATA+C+CAT+G-TMR) (TIB MOLBIOL) RT-qPCR was performed using the following cycle ...
-
No products found
because this supplier's products are not listed.
Helen J. von Richthofen, et al.,
bioRxiv - Immunology 2022
Quote:
... Cells were treated 2h at 37°C with neutrophil elastase (Elastin Products Company), cathepsin G (Biocentrum) ...
-
No products found
because this supplier's products are not listed.
Emilie Pondeville, et al.,
bioRxiv - Genetics 2019
Quote:
... Hemocytes were incubated at 4°C overnight with a rat anti-mCD8 antibody (Ancell) diluted 1:100 or a rabbit anti-PPO2 (Fraiture ...
-
No products found
because this supplier's products are not listed.
Natasha M. O’Brown, et al.,
bioRxiv - Neuroscience 2021
Quote:
Larvae (7 dpf) were anesthetized with tricaine and injected with 2.3 nl of 5 nm NHS-activated gold nanoparticles (Cytodiagnostics: CGN5K-5-1, ~1.114 particles/ml in PBS) just as for the fluorescent tracer injections ...
-
No products found
because this supplier's products are not listed.
Eva Gracia Villacampa, et al.,
bioRxiv - Genetics 2020
Quote:
... the slides were dried at 40°C in a hybridization oven (HB-1000 Hybridizer, LabRepCo) for 1 hour and 45 min ...
-
No products found
because this supplier's products are not listed.
Matthew J. Foulkes, et al.,
bioRxiv - Immunology 2019
Quote:
... The c-Jun N-terminal kinase inhibitor SP600125 was obtained from StressMarq Biosciences (Victoria, Canada), and tanshinone IIA (TIIA ...
-
No products found
because this supplier's products are not listed.
Y Audrey Hay,
bioRxiv - Neuroscience 2021
Quote:
... France) mice temperature was monitored and maintained at 34-36 °C using a heating blanket (Basi). A small burr hole was drilled at LDTg coordinates AP = -4.92 mm and ML = 1 mm and an optical fiber (300 μm ...
-
No products found
because this supplier's products are not listed.
Toshiyuki Oda, et al.,
bioRxiv - Immunology 2022
Quote:
... and 4 µl of HMC buffer containing cytochrome c-stabilized 15-nm colloidal gold (BBI Solutions) was applied for attachment of fiducial markers (Oda et al. ...
-
No products found
because this supplier's products are not listed.
Alyssa N Coyne, et al.,
bioRxiv - Neuroscience 2020
Quote:
Two rabbits were immunized with the peptide antigen C-Ahx-(GP)8-amide (21st Century Biochemicals). Specificity of antiserum versus pre-immune serum was verified by peptide dot blot and Western blot of HEK cells overexpressing GFP-tagged dipeptide repeat proteins (plasmids kindly provided by L ...
-
No products found
because this supplier's products are not listed.
Tamás Bakos, et al.,
bioRxiv - Immunology 2024
Quote:
... Soluble terminal C complex (sTCC, sC5b9) was measured with an ELISA kit from Svar Life Science AB (Malmö ...
-
No products found
because this supplier's products are not listed.
Tomoki Togashi, et al.,
bioRxiv - Bioengineering 2023
Quote:
... hPC antigen (hPC:Ag) was measured using the Human Protein C AssayMaxTM ELISA Kit (Assaypro, St. Charles, MO) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Yannick O Alexandre, et al.,
bioRxiv - Immunology 2020
Quote:
... were coated overnight at 4°C with 10 μg/ml HSV-1 inactivated Vero cell extract (Advanced Biotechnologies). Unbound protein was washed away (PBS 0.05% Tween20 ...
-
No products found
because this supplier's products are not listed.
Han Hao, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The following antibodies were used: VGAT C-terminal antibody (rabbit polyclonal #AB-N44, Advance Targeting System; 1:200); VGAT N-terminal antibody (rabbit polyclonal 131002 ...
-
No products found
because this supplier's products are not listed.
Vera Kovaleva, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Cells were incubated overnight at +4°C with the following primary antibodies: anti-MANF rabbit pAb (Icosagen, 310-100), anti-IRE1α rabbit mAb (CST ...
-
No products found
because this supplier's products are not listed.
Charlène Iltis, et al.,
bioRxiv - Immunology 2021
Quote:
... membranes were stripped at 4°C in agitation using Antibody stripping buffer 1X for 10 minutes (Gene Bio-Application). Protein bands were quantified using ImageJ software ...
-
No products found
because this supplier's products are not listed.
Tobias Butelmann, et al.,
bioRxiv - Bioengineering 2021
Quote:
... an LM was taken out from the medium (30 °C) and weighed on a precision scale (Adam Equipment, UK). To determine the dry weight of the cells ...
-
No products found
because this supplier's products are not listed.
Justin L. Tosh, et al.,
bioRxiv - Neuroscience 2020
Quote:
... ranging from 3678 to 3.6 pg/ml of a synthetic dimer with the Aβ1-11 sequence (DAEFRHDSGYE) C-terminally linked via a cysteine residue (28) was ordered from rPeptide and supplied by Stratech.
-
No products found
because this supplier's products are not listed.
Timothy O'Leary, et al.,
bioRxiv - Biochemistry 2021
Quote:
Cells were washed with DPBS and incubated with cholPEGNTA (Nanocs # PG2-CSNT-2k; 10 μM, 1 hr, 37°C) After washing twice with DPBS ...
-
No products found
because this supplier's products are not listed.
Peter A. van der Ley, et al.,
bioRxiv - Immunology 2021
Quote:
The prefusion stabilized HexaPro spike protein27 with and without a 3xGGGS spacer and mCRAMP sequence added to the C-terminus were produced by Celonic AG (Basel ...
-
No products found
because this supplier's products are not listed.
Marissa M. Gaskill, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... attP40{nos-Cas9}/CyO for the N-terminal line and y1 M{vas-Cas9.RFP-}ZH-2A w1118 (BDSC#55821) for the C-terminal line by BestGene Inc ...
-
No products found
because this supplier's products are not listed.
Brian C. Russo, et al.,
bioRxiv - Microbiology 2021
Quote:
... The cells were then incubated overnight at 4°C with rabbit anti-Shigella conjugated to FITC (ViroStat, catalog no. 0903). The next day ...
-
No products found
because this supplier's products are not listed.
Cornelia Blume, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Data were normalised to the geometric mean of the housekeeping genes (ubiquitin C and glyceraldehyde 3-phosphate dehydrogenase, probe-based duplex primer mix, PrimerDesign) and fold change in gene expression relative to controls was determined using the ΔΔCt method ...
-
No products found
because this supplier's products are not listed.
Guizhen Fan, et al.,
bioRxiv - Biophysics 2022
Quote:
... Membranes were probed with a rabbit polyclonal antibody against the C-terminal 19aa of IP3R1 (CT1; #ARC154, Antibody Research Corporation) at a 1:1000 dilution ...
-
No products found
because this supplier's products are not listed.
Emmeline L. Blanchard, et al.,
bioRxiv - Bioengineering 2020
Quote:
... The RNA was heat denatured at 65°C for 10 minutes before capping with a Cap-1 structure using guanylyl transferase and 2’-O-methyltransferase (Aldevron). mRNA was then purified by lithium chloride precipitation ...
-
No products found
because this supplier's products are not listed.
Jugal Mohapatra, et al.,
bioRxiv - Biochemistry 2021
Quote:
... and heated to 90 °C for 2 min while bubbling with nitrogen gas in N,N-dimethylformamide (DMF, Oakwood Chemical). Fmoc deprotection was carried out with 20% piperidine (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Jamie Orr, et al.,
bioRxiv - Plant Biology 2023
Quote:
... The captured protein was divided in two and half was incubated for two hours at 37°C with broad spectrum deubiquitinating enzyme (DUB; LifeSensors). The results of the assay were determined via SDS PAGE and western blotting as described above.
-
No products found
because this supplier's products are not listed.
Alyssa F. Pybus, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Samples were incubated at 4°C overnight with primary antibodies diluted in blocking buffer: GFAP (1:100, Diagnostic Biosystems Mob064), NeuN (1:200 ...
-
No products found
because this supplier's products are not listed.
Isabelle Petit-Härtlein, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 0.1 µL of protein solution and 0.1 µL of reservoir were mixed to equilibrate against 45 µL reservoir solution at 20°C in 96-well CrystalDirect plates (MiTeGen) and automatically imaged in RockImager robot (Formulatrix) ...
-
No products found
because this supplier's products are not listed.
Tomasz Zieliński, et al.,
bioRxiv - Biophysics 2022
Quote:
... and kept in the CO2 (37°C and 5%CO2/95% air atmosphere) for 24 hours in the DMEM (ATCC, LGC Standards) supplemented with 10% FBS ...
-
No products found
because this supplier's products are not listed.
Genevieve. McCluskey, et al.,
bioRxiv - Immunology 2021
Quote:
The effect of FH on protein C (PC) activation was monitored by combining FH (0.5-125nM) with 1.75nM thrombin and 200nM PC (Haematologic Technologies, Inc., USA) in the absence or presence of 10nM soluble recombinant TM (R&D Systems/Biotechne ...
-
No products found
because this supplier's products are not listed.
Yujiao He, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Raji B lymphoma cells were incubated for 30 min at 37°C in the presence or absence of SEE (100 ng/ml; Toxin Technology). The cells were washed with PBS and were mixed with Jurkat T cells at a ratio of 1:1 ...
-
No products found
because this supplier's products are not listed.
Anna Vilalta, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Proteins in storage buffer were concentrated to between 5-10 mg/ml for use and tested for endotoxin with the EndoZyme recombinant Factor C assay (Hyglos GmbH). Endotoxin levels in all assays were maintained at < 0.1 EU/ml.
-
No products found
because this supplier's products are not listed.
Henriette Frikke-Schmidt, et al.,
bioRxiv - Physiology 2020
Quote:
... The plates for the islets were placed on a mat heated at 37 °C on a H101A ProScan motorized stage (Prior Scientific Instruments Ltd ...
-
No products found
because this supplier's products are not listed.
Alan P. R. Lorenzetti, et al.,
bioRxiv - Systems Biology 2022
Quote:
... Cell pellets were resuspended in Milli-Q water and disrupted at 4°C using ceramic beads (Mo Bio Laboratories) and a Precellys 24 homogenizer (Bertin Corp). Protein content was determined by bicinchoninic acid assay (BCA ...
-
No products found
Tao Cheng, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... 15 minutes at 4°C) from blood isolated by sub-mandibular bleeding and used for the BUN assay (BUN-Urea, BioAssay Systems) following the manufacturer’s protocol.
-
No products found
because this supplier's products are not listed.
Grigorios Fanourgakis, et al.,
bioRxiv - Genetics 2024
Quote:
... After washing cells were incubated with 20 μg/ml with Hoechst 33342 (Thermo Fischer Scientific H3570) for 1 hour at 32°C with constant shaking and protected from light and then with 30nM DRAQ7 (Biostatus DR71000) for 5 minutes on bench ...
-
No products found
because this supplier's products are not listed.
Lauren Rylaarsdam, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Organoid pieces were incubated for 75 minutes on a 95 RPM orbital shaker in a 37°C incubator and triturated using wide bore tips (Rainin, 30389218) every 30 minutes to help break up pieces ...
-
No products found
because this supplier's products are not listed.
Hui Zhang, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... Samples were boiled at 100 °C in a metal bath for 10 min in protein loading buffer (CoWin Biosciences, Beijing, China) and equal amount proteins were separated by 10% SDS-PAGE gel ...
-
No products found
because this supplier's products are not listed.
Claudia Ctortecka, et al.,
bioRxiv - Biochemistry 2024
Quote:
... the proteoCHIP EVO 96 is inserted into the cellenONE® using a dedicated holder (C-PEVO-96-CHB, Scienion, Berlin, Germany), 150nL of master mix (0.2% DDM (D4641-500MG ...
-
No products found
because this supplier's products are not listed.
Aldo E. García-Guerrero, et al.,
bioRxiv - Microbiology 2024
Quote:
... Membranes were blocked at room temperature for 1 hour in 5% skim milk/PBS and probed overnight at 4°C with 1:1000 dilutions of rabbit anti-HSP60 (Novus NBP2-12734) and goat anti-GFP (Antibodies.com A121560) antibodies ...
-
No products found
because this supplier's products are not listed.
Tongcui Ma, et al.,
bioRxiv - Microbiology 2023
Quote:
... or conjugated in-house with X8 antibody-labeling kits (Standard BioTools) and stored at 4°C in Antibody Stabilizer (Boca Scientific) supplemented with 0.05% sodium azide ...
-
No products found
because this supplier's products are not listed.
Chloe C. Ence, et al.,
bioRxiv - Microbiology 2024
Quote:
... The cultures were incubated at 37°C with 5% CO2 and routinely checked for mycoplasma contamination using the e-Myco Plus kit from Intron Biotechnology. Human hepatocellular carcinoma cells (HepG2 ...
-
No products found
because this supplier's products are not listed.
Wakana Sato, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... at 37°C for 1 hour and agarose gel-purified with GenCatch Advanced Gel Extraction Kit (Epoch Life Science, Inc., No. 2260050). eGFP fluorescence was measured at λex 488 nm and λem 509 nm with plate reader PMT setting “medium” and 6 reads per well ...
-
No products found
because this supplier's products are not listed.
Mathew Miller, et al.,
bioRxiv - Molecular Biology 2022
Quote:
MULTI-ARRAY Standard 96-well plates (Meso Scale Diagnostics, Rockville, Maryland) were coated overnight at 4°C with K1 anti-dsRNA mouse monoclonal antibody (SCICONS, Budapest, Hungary). Plates were blocked using 5% MSD Blocker A (Meso Scale ...
-
No products found
because this supplier's products are not listed.
Thekla Cordes, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... The cell suspension was centrifuged at 20,000 g for 10 min at 4 °C and 20 µl of the supernatant was used for protein quantification using BCA protein assay kit (Cat. #G1002, Lamda Biotech. Inc) as per manufacturer’s instruction.
-
No products found
because this supplier's products are not listed.
Yuichiro Honda, et al.,
bioRxiv - Pathology 2020
Quote:
... The sections were blocked with 5% bovine serum albumin in PBS for 60 min and incubated overnight at 4°C with a mouse anti-CD-11b primary antibody (1:2000; BMA Biomedicals, Augst, Switzerland) or a rabbit polyclonal anti-dystrophin primary antibody (1:1000 ...
-
No products found
Bikash Manandhar, et al.,
bioRxiv - Cell Biology 2023
Quote:
Cell surface F1-ATPase β-subunit in Ins-1E cells was blocked by pre-incubation (37 °C, 15 min) with IF1 (final concentration 10 µg/mL, Creative BioMart, Shirley, NY) in KRBH/0.1 % (w/v ...
-
No products found
because this supplier's products are not listed.
Julie Van Coillie, et al.,
bioRxiv - Immunology 2022
Quote:
... IL-6 levels in the supernatant were measured by enzyme-linked immunosorbent assay (ELISA) using IL-6 CT205-c and CT205-d antibody pair (U-CyTech, Utrecht, the Netherlands) as described previously (1).
-
No products found
because this supplier's products are not listed.
Lisanne de Vor, et al.,
bioRxiv - Microbiology 2021
Quote:
... antibodies were buffer exchanged into conjugation buffer and incubated at 37°C for 1.5 h with a 5-fold molar excess of bifunctional CHXA” (Macrocyclics, prepared less than 24 h before use). The mAb-CHXA” conjugate was then exchanged into 0.15 M ammonium acetate buffer to remove unbound CHXA” and subsequently incubated with approximately 150 kBq 111In (purchased as [111In]InCl3 from CuriumPharma ...