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No products found
because this supplier's products are not listed.
Zachary T. Olmsted, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 24 - 48 h neurosphere (NS) cultures were formed in 6-well plates (CELLTREAT) using a Scilogex MX-M microplate mixer at ~120 rpm and freshly seeded ...
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No products found
because this supplier's products are not listed.
Jesse V Veenvliet, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Gastruloids were washed twice with PBS and then fixed in 4% PFA for 1 h in glass vials (Wheaton 224882) at 4°C on a roller ...
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No products found
because this supplier's products are not listed.
Stephen J. DeCamp, et al.,
bioRxiv - Biophysics 2020
Quote:
Polymerized polyacrylamide gels were first treated with 440 μL of a 1:50 (v/v) mixture of Sulfosuccinimidyl 6-(4'-azido-2'-nitrophenylamino)hexanoate (Sulfo-SANPAH) (ProteoChem) and 50 mM HEPES solution under a UV light for 10 minutes ...
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No products found
because this supplier's products are not listed.
Adrià Razzauti, Patrick Laurent,
bioRxiv - Cell Biology 2021
Quote:
To analyze co-localization of animals co-expressing TSP-6-wrmScarlet and GCY-22-mEGFP in ASER neuron we used Imaris software Version 9 (Oxford Instruments, Zurich, Switzerland). We first established a ROI containing the cilia and their base ...
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No products found
because this supplier's products are not listed.
Geoffrey L. Rogers, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
... 9 × 106 293AAV cells (Cell Biolabs) were seeded in 15 cm diameter dishes to achieve 70-80% confluence on the day of transfection ...
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No products found
because this supplier's products are not listed.
Alexander Popov, et al.,
bioRxiv - Neuroscience 2021
Quote:
... for one h at room temperature and then incubated overnight at four °C with primary mouse antibodies for ezrin (Antibodies-Online, ABIN5542456, Aachen, Germany), with primary rabbit antibodies for GLT-1 (Abcam ...
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No products found
because this supplier's products are not listed.
Xuedan Sun, et al.,
bioRxiv - Cell Biology 2019
Quote:
Cells were incubated in serum-free DMEM for 24 h and IL-6/8 levels determined in supernatants using the femtoELISA™ HRP Kit (G-Biosciences).
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No products found
because this supplier's products are not listed.
Kevin D. Whitley, et al.,
bioRxiv - Microbiology 2021
Quote:
... A 9 mm diameter silicone gasket (Grace Bio-Labs) was then placed over the PDMS microholes ...
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No products found
because this supplier's products are not listed.
Liliana D Ordonez, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... anti-K18 (1/5; Progen Biotechnik), anti-ΔNp63 (1:100 ...
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No products found
because this supplier's products are not listed.
Huifang Jia, et al.,
bioRxiv - Molecular Biology 2022
Quote:
Total RNA was extracted from less than 100 mg of 10-d-old seedlings that were grown vertically on half-strength MS plates or treated with 50 μM AA or an equal volume of DMSO for 4 h in liquid MS medium using RNAprep Pure Plant Plus Kit (TIANGEN, DP441). First-strand cDNA was synthesized using a HiScript® II cDNA Synthesis Kit (Vazyme R212-02 ...
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No products found
because this supplier's products are not listed.
Xian Zhou, et al.,
bioRxiv - Immunology 2020
Quote:
... Human B cell proliferation was measured by 3[H]-thymidine incorporation (1 μCi/mL) (American Radiolabeled Chemicals).
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No products found
because this supplier's products are not listed.
Nathaniel W. Snyder, et al.,
bioRxiv - Biochemistry 2021
Quote:
... The immunoblot was probed with 1:1000 PTGR1 rabbit antibody for 24 h (Aviva Systems Biology, San Diego, CA) and a horseradish peroxidase-conjugated anti-rabbit secondary antibody for 1 h ...
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No products found
because this supplier's products are not listed.
Kavya Prasad, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... mounted in Vectashield Antifade solution (4’,6 diamidino-2-phenylindole, Vector laboratories H-1200, Axxora/Alexis, Lörrach, Germany) with DAPI and cover with 24×60 mm coverslip sealed with a nail polish ...
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No products found
because this supplier's products are not listed.
Julie Firmin, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Embryos are placed in pre-equilibrated (at least 4 h at 37°C, 5% O2, 5% CO2) CSCM-C medium (Irvine Scientific) covered with mineral oil (Irvine Scientific ...
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Separase inhibitor
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Cat# 3082.0, SKU# 3082-10 mg,
10mg, US $121.00 / EA, EURO, €110 / EA
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Isabel R.K. Kuebler, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... The MCH receptor antagonist 6-(4-Chloro-phenyl)-3-[3-methoxy-4-(2-pyrrolidin-1-yl-ethoxy)-phenyl]-3H-thieno[3,2-d]pyrimidin-4-one (GW803430) (Axon Medchem, Groningen, Netherlands) was freshly prepared the day of the treatment ...
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No products found
because this supplier's products are not listed.
Hamid Keshmiri, et al.,
bioRxiv - Biophysics 2023
Quote:
... U2OS cells were treated with 10 µM trichostatin A (TSA) for 6h (AbMole M1753), 25 µM camptothecin for 1h (Sigma-Aldrich C9911) ...
-
No products found
because this supplier's products are not listed.
Adar Sonn-Segev, et al.,
bioRxiv - Biophysics 2019
Quote:
... and the supernatant applied for 3 h at 4°C to 5 mL of Toyopearl AF-Chelate-650M resin (Tosoh) precharged with Ni2+-ions ...
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No products found
because this supplier's products are not listed.
Uxia Gurriaran-Rodriguez, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... After 6h of transfection cells were treated overnight with PORCN inhbibitor diluted in DMSO (AdooQ) at two different concentrations 100nM and 500nM in fresh media ...
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No products found
because this supplier's products are not listed.
Qing-Tao Meng, et al.,
bioRxiv - Neuroscience 2021
Quote:
... chicken anti-NF-H (1:2000, EnCor Biotechnology, CPCA-NF-H), rabbit anti-βIII-Tubulin (1:2000 ...
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No products found
because this supplier's products are not listed.
Tomoya Sasahara, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Counterstaining was carried out with 4’,6-diamidino-2-phenylindole (DAPI, 1:500; Dojindo Molecular Technologies, Kumamoto, Japan). Fluorescence images were acquired with a confocal laser-scanning microscope LSM710 (Carl Zeiss ...
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No products found
because this supplier's products are not listed.
Danit Wasserman, et al.,
bioRxiv - Cell Biology 2019
Quote:
... ii) mock or 0.4 mg/ml of one of the following unlabled Ubiqutin variants: WT (Boston Biochem; #U100-H), Lys11 to Arg mutant (Boston Biochem ...
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No products found
because this supplier's products are not listed.
Andry Andrianarivelo, et al.,
bioRxiv - Neuroscience 2021
Quote:
9-tert-butyl doxycycline hydrochloride (9-TB-dox; Tebu-bio, Le Perray-en-Yvelines, France) was dissolved in a saline solution containing DMSO (5% ...
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No products found
because this supplier's products are not listed.
Andrew J. Lutkewitte, et al.,
bioRxiv - Physiology 2020
Quote:
... Insulin was measured in serum from 6 h-fasted mice using an ELISA (Crystal Chem, Downers Grove, IL). For protein studies livers were immediately harvested ...
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No products found
because this supplier's products are not listed.
Zhenwu Luo, et al.,
bioRxiv - Immunology 2021
Quote:
... The membrane was blocked for 1 h at room temperature and incubated overnight at 4°C with CREM/ICER antibody (clone 3B5) (LifeSpan BioSciences, Seattle, WA). Mouse anti-human β-actin was used as the reference ...
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No products found
because this supplier's products are not listed.
Mathieu Paquette, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... cells were incubated for 2 h in medium containing one of the following reagents: Dimethyl sulfoxide (DMSO) (0.1%) (Bioshop Canada, DMS666), Torin1 (1 μM ...
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No products found
because this supplier's products are not listed.
F. Phil Brooks III, et al.,
bioRxiv - Neuroscience 2024
Quote:
... a stack of one 5 mm and two 3 mm round cover glass (Thomas Scientific, 1217N66), pre-glued by optical glue (Norland 61) ...
-
No products found
because this supplier's products are not listed.
Souade Ikhlef, et al.,
bioRxiv - Biochemistry 2021
Quote:
... and 16:0/16:0-PI(4,5)P2 (1,2-dipalmitoyl-sn-glycero-3-phospho-(1′-myo-inositol-4′,5′-bisphosphate)) were purchased from Echelon Biosciences.
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No products found
because this supplier's products are not listed.
Abdulbasit Amin, et al.,
bioRxiv - Physiology 2022
Quote:
Blood was collected from 6 – 7 h fasted mice to quantify the concentration of insulin in the serum using an insulin ELISA kit (ALPCO). The medium of adipocytes was cleared and used to evaluate the levels of TNF ...
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No products found
because this supplier's products are not listed.
Fulai Zhou, et al.,
bioRxiv - Biophysics 2021
Quote:
Spodoptera frugiperda 9 (Sf9) insect cells (Expression Systems) were grown in ESF 921 serum-free medium (Expression Systems ...
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No products found
because this supplier's products are not listed.
Uljana Kravchenko, et al.,
bioRxiv - Microbiology 2020
Quote:
... The fixed cells were harvested (8000 g, 5 min, 4 °C) and resuspended in 1 mL of ExtractRNA Reagent (Evrogen, Russia) and the subsequent procedures were performed according to manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Flavio R. Palma, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... for 2 h at 4 °C and finally stained with anti-8-oxo-dG (Trevigen, #4354-MC-050), followed by incubation with secondary antibody Alexa647 (Invitrogen #A28181) ...
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No products found
because this supplier's products are not listed.
Takahide Matsui, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Bound antibodies were detected with goat anti-mouse IgG (H+L) 5-nm colloidal Gold particles (BBI Solutions) and rinsed with 0.1 M phosphate buffer (pH 7.4 ...
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No products found
because this supplier's products are not listed.
Justine Cristante, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... IMPACT Mouse FELASA and MKPV testing and was authenticated by CellCheck human 9 Test (9 Marker STR Profile and Inter-species Contamination Test) (IDEXX Analytics, Kornwestheim, Germany). H295R cells stably expressing a doxycycline inducible β-Catenin shRNA (pTer1 shRNA vector ...
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No products found
because this supplier's products are not listed.
Subhrangshu Guhathakurta, et al.,
bioRxiv - Neuroscience 2020
Quote:
... from days 1-6 and 3-μM CHIR99021(Reprocell, 04-0004) from days 3-12 ...
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No products found
because this supplier's products are not listed.
Vivian K. Rojas, et al.,
bioRxiv - Microbiology 2024
Quote:
... The chromogenic molecule X-Phos (5-bromo-4-chloro-3-indolyl phosphate, Chem-Impex) was added to agar plates with the purpose of detecting S ...
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No products found
because this supplier's products are not listed.
Siyuan Zhao, et al.,
bioRxiv - Neuroscience 2021
Quote:
... (9) Spin-coating SU-8 precursor (SU-8 2025, MicroChem) at 4000 rpm ...
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No products found
because this supplier's products are not listed.
James W. Smyth, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... and twice with PBS (2 x 5 min) before using One-step TUNEL In Situ Apoptosis kit (Green Elab Fluor® 488; Elabscience), according to manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Robert S. Porter, et al.,
bioRxiv - Molecular Biology 2023
Quote:
One μg of recombinant (Epicypher) or cellular nucleosomes (see protein purification above ...
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No products found
because this supplier's products are not listed.
Ye Sun, et al.,
bioRxiv - Neuroscience 2019
Quote:
... for 40 h and nanogold conjugated secondary antibody (1:100, #2003, Nanoprobes, NY) for 16 h ...
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No products found
because this supplier's products are not listed.
Daniel J. Modulevsky, et al.,
bioRxiv - Bioengineering 2022
Quote:
... a pledgets of surgifoam (5 mm2) were punched out and soaked in 4% FluoroGold (Fluorochrome) in saline ...
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No products found
because this supplier's products are not listed.
Benjamin C. Hunt, et al.,
bioRxiv - Microbiology 2023
Quote:
... Tissues were homogenized using a Bullet Blender 5 Gold (Next Advance, Speed 8, 4 minutes). Bladders were treated to two cycles to ensure full homogenization ...
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No products found
because this supplier's products are not listed.
Valeria Rudman-Melnick, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... UIR and UUO whole kidney lysates (n=4-6 per group) with RNA Stat-60 extraction reagent (Amsbio, CS-111) and purified using the GeneJET RNA purification kit (ThermoFisher Scientific ...
-
No products found
because this supplier's products are not listed.
Alexander V. Balatskiy, et al.,
bioRxiv - Synthetic Biology 2024
Quote:
... 6–8-week-old female mice were anesthetized with 5% isoflurane (Baxter, 10019036040) in an induction chamber (SomnoSuite, Kent Scientific), and then placed in the supine position onto a heating pad (Digital stereotaxic instrument ...
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No products found
because this supplier's products are not listed.
Nicole B. Webster, et al.,
bioRxiv - Developmental Biology 2021
Quote:
Total RNA was extracted from mixed stage 1–9 embryos and larvae using the RNA Trizol extraction protocol (Molecular Research Center, Inc.) or the RNeasy Mini Kit (Qiagen cat ...
-
No products found
because this supplier's products are not listed.
Yoon-Suk Kang, James E. Kirby,
bioRxiv - Microbiology 2022
Quote:
... supplemented with 9% iron-supplemented calf serum (Gemini Bio-Products, West Sacramento, CA), was seeded into white 96-well or clear 6-well tissue culture plates in the same medium ...
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No products found
because this supplier's products are not listed.
Ori Maller, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... anti-cytokeratin 5 antibody (Fitzgerald, Cat# 20R-CP003, dilution 1:400), anti-vimentin antibody (Cell Signaling ...
-
No products found
because this supplier's products are not listed.
Natascia Marino, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... and BeadBug 6 homogenizer (Benchmark Scientific) in a cold room at the following conditions ...
-
No products found
because this supplier's products are not listed.
Silvia Ferrara, et al.,
bioRxiv - Microbiology 2020
Quote:
... The lungs were washed with three one ml of RPMI-1640 (Euroclone) with protease inhibitors (Complete tablets ...
-
No products found
because this supplier's products are not listed.
Fabien Thery, et al.,
bioRxiv - Cell Biology 2021
Quote:
... at 10 ng/mL for 24 h (#11343596, #11343524, Immunotools). Each condition (2 baits ...
-
No products found
because this supplier's products are not listed.
Qi Feng, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... and incubated overnight at 4°C with primary antibodies against DCBLD1 (1:30, Atlas antibody, Bromma, Sweden) and CD31 (1:100 ...