-
No products found
because this supplier's products are not listed.
Giuseppe Gangarossa, et al.,
bioRxiv - Neuroscience 2019
Quote:
... and 2-(4-morpholino)-8-phenyl-4H-1-benzopyran-4-one (LY294002, 10µM) (Tocris) were dissolved in DMSO and then added in the external solution at a final concentration of DMSO of 0.001-0.04% ...
-
No products found
because this supplier's products are not listed.
Santiago Ojea Ramos, et al.,
bioRxiv - Neuroscience 2020
Quote:
A stock solution (74.8 mM) of the MEK inhibitor 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one (PD98059, PD hereafter, Sigma-Aldrich, Argentina) was conserved at −20 °C and freshly diluted in DMSO to the desired concentration (11.22 mM ...
-
No products found
because this supplier's products are not listed.
Aleksandar Bartolome, et al.,
bioRxiv - Biochemistry 2023
Quote:
... anti-Proteasome 20S alpha 1+2+3+5+6+7 (1:200, Abcam, ab22674), anti-GFP (Santa Cruz ...
-
No products found
because this supplier's products are not listed.
Elisangela Bressan, et al.,
bioRxiv - Genomics 2023
Quote:
The PPMI iPSC lines were thawed and grown on matrigel (Corning)-coated plates with Essential 8 Flex (E8, Batches 1, 2 and 3) or Essential 6 (E6, Batches 4 and 5) media (both Gibco) for about one month (5 passages) ...
-
No products found
because this supplier's products are not listed.
Paul A. S. Sheppard, et al.,
bioRxiv - Neuroscience 2022
Quote:
... or 10ng/side of PI3K inhibitor 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY294002; Santa Cruz Biotechnology, Dallas, TX); or vehicle (50% dimethyl sulfoxide [DMSO] in 0.9% NaCl solution ...
-
No products found
because this supplier's products are not listed.
Athéna Labeau, et al.,
bioRxiv - Microbiology 2021
Quote:
... and pVSV-G (4:3:1 ratio) using calcium phosphate transfection (Promega) following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Ji-Won Seo, et al.,
bioRxiv - Bioengineering 2021
Quote:
... C57BL/6 mice (7-8 weeks old; Charles River Laboratories) were subsequently subcutaneously injected with 100 μL of 3 x 106 B16F10 cells/mL in PBS above the right hind leg ...
-
No products found
because this supplier's products are not listed.
Darrell Pilling, et al.,
bioRxiv - Immunology 2022
Quote:
... 7-8 week old 20-25 g male and female C57BL/6 mice (Jackson Laboratories, Bar Harbor, ME) were given an oropharyngeal aspiration of 3 U/kg bleomycin (2246-10 ...
-
No products found
because this supplier's products are not listed.
Harold P. Hodgins, et al.,
bioRxiv - Genomics 2022
Quote:
... Mice (CD-1 strain, female, purchased from Envigo, 6-7 weeks old, 25–28 g, n=3) were anesthetized with isoflurane (3–4% ...
-
No products found
because this supplier's products are not listed.
Elizabeth Min, et al.,
bioRxiv - Cell Biology 2019
Quote:
... P-Smad 1/5/8 (1:1000; Cell Signaling; #13820), P-Smad 2/3 (1:1000 ...
-
No products found
because this supplier's products are not listed.
Jun Feng, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... The cultures were then inoculated into DM29-fructose (5 g L- 1) and DM29-glucose (5 g L-1) media in a 96-well plate (Greiner Bio-One) to an initial OD600 of 0.05 ...
-
No products found
because this supplier's products are not listed.
Teresa Neuwirth, et al.,
bioRxiv - Immunology 2024
Quote:
... Each patient was also stained with one of TotalSeq™-C0251/2/3/4 Antibody (Biolegend, Cat: 394661/3/5/7, 1:100) for sample multiplexing ...
-
No products found
because this supplier's products are not listed.
Nicolas Wieder, et al.,
bioRxiv - Cell Biology 2023
Quote:
... template switching (5’- AAGCAGTGGTATCAACGCAGAGTACrGrG+G-3’, Qiagen), and ISPCR (5’-AAGCAGTGGTATCAACGCAGAGT-3’ ...
-
No products found
because this supplier's products are not listed.
Pei-Yu Chu, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... The 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one (PD98059, MEK inhibitor) and STAT3 inhibitor VI were from Calbiochem (Darmstadt, Germany). The EGF was from Millipore (Billerica ...
-
No products found
because this supplier's products are not listed.
Kazuki Yoshizumi, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... blots were hybridized with 10 pmol/ml DIG-labeled (CAG)7 (5′-gcAgCagcAgca-3′) at 70°C for 4 h in hybridization buffer (5 × SSC, 1% block solution [Roche, Switzerland] ...
-
No products found
because this supplier's products are not listed.
Basila Moochickal Assainar, et al.,
bioRxiv - Biophysics 2023
Quote:
... 0.5% NBD-PC (1-palmitoyl-2-(6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl)-sn-glycero-3-phosphocholine (Avanti Polar Lipids, Inc.)) ...
-
No products found
because this supplier's products are not listed.
Yi-Wei Huang, et al.,
bioRxiv - Cell Biology 2024
Quote:
COS-7 cells (∼ 3-5 x 104) were grown on 8-well chamber glass slides (BD Falcon 8 chamber tissue culture-treated glass slides ...
-
No products found
because this supplier's products are not listed.
Mukaddes Izci, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
When the tumor sizes reached to 80 mm3 animals were treated with a PFKFB3 inhibitor 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one) (3PO) (Sigma-Aldrich, Merck, Overijse, Belgium). The animals received intraperitoneal (i.p. ...
-
No products found
because this supplier's products are not listed.
Jia-Ying Su, et al.,
bioRxiv - Genetics 2024
Quote:
... m7G(5’)ppp(5’)G RNA Cap Structure Analog (New England Biolabs, 4:1 to GTP) was supplemented to the in vitro transcription reaction ...
-
No products found
because this supplier's products are not listed.
Sylvia Krippner, et al.,
bioRxiv - Cell Biology 2020
Quote:
... HEK293T cells were transfected using calcium phosphate method with 4-6 µg plasmid DNA and 8 µg of each pMD2.G and psPAX2 (Addgene). After 48 h ...
-
No products found
because this supplier's products are not listed.
Sabrina Summer, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... and boiled for 5 min at 95°C were separated on 6-8% polyacrylamide/7 M Urea denaturing gel in 1× TBE and transferred onto an Amersham HybondN+ membrane (GE Healthcare). RNA was UV-crosslinked to the membrane ...
-
No products found
because this supplier's products are not listed.
Hemakumar M. Reddy, et al.,
bioRxiv - Genomics 2021
Quote:
... The proteins were separated in the first dimension on 4-7 and 5-8 IPG strips (Bio-Rad). The strips were then loaded onto 8-20% gradient PAGE for separation on the second dimension ...
-
No products found
because this supplier's products are not listed.
Zachary T. Olmsted, Janet L. Paluh,
bioRxiv - Developmental Biology 2022
Quote:
... Cultures were passaged 1:6 in 6-well plates every 4-7 days using Gentle Cell Dissociation Reagent (GCDR, STEMCELL Technologies). Cells were cryopreserved in mFreSR.
-
No products found
because this supplier's products are not listed.
Anaísa V. Ferreira, et al.,
bioRxiv - Immunology 2023
Quote:
... 1 or 5 µM 7-HDHA (Cayman Chemical), 1 or 10 nM Leukotriene B4 (Cayman Chemical ...
-
No products found
because this supplier's products are not listed.
Annika Ullrich, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...
-
No products found
because this supplier's products are not listed.
Alison Dumont, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Caspase 3/7 dye (4440, Sartorius, 1/1000) and/or propidium Iodide (PI ...
-
No products found
because this supplier's products are not listed.
Jordan J. Aoyama, Medha Raina, Gisela Storz,
bioRxiv - Microbiology 2021
Quote:
... was separated on denaturing 8% polyacrylamide gels containing 6 M urea (1:4 mix of Ureagel Complete to Ureagel-8 (National Diagnostics) with 0.08% ammonium persulfate ...
-
No products found
because this supplier's products are not listed.
Simon P. Fraessle, et al.,
bioRxiv - Immunology 2022
Quote:
... 5 ng ml−1 recombinant human IL-7 (Peprotech) and 5 ng ml−1 IL-15 ...
-
No products found
because this supplier's products are not listed.
Yang Wang, et al.,
bioRxiv - Bioengineering 2020
Quote:
... with 1-L buffer containing 3 g of Tris base (VWR international), 14.4 g of glycine (VWR international ...
-
No products found
because this supplier's products are not listed.
Jinyang Wang, et al.,
bioRxiv - Biochemistry 2023
Quote:
... shRNAs against mouse ACOT12 (#5, #6 and #7) and mouse ACOT8 (#1 and #2) were also constructed in pAdEasy-1 (Stratagene) for adenovirus packaging based on The AdEasyTM Technology (He et al. ...
-
No products found
because this supplier's products are not listed.
Alexis M. Crockett, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Antibodies were diluted in 3% normal donkey serum (monoclonal mouse anti-mouse GFAP 1:2000, Sigma G-A-5; polyclonal rabbit anti-human IL-6 1:50, Proteintech), and incubated overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Osamu Hisano, Takashi Ito, Fumihito Miura,
bioRxiv - Genomics 2021
Quote:
... 1 μL of 0.05 mM 7-propargylamino-7-deaza-ddATP-6-FAM (Jena Bioscience, Jena, Germany), 0.4 μL of internal standard solution (see Supplementary Methods) ...
-
No products found
because this supplier's products are not listed.
Katarzyna Wacnik, et al.,
bioRxiv - Microbiology 2021
Quote:
... 3 µl of 1 M NaOH and 6 µl of Cell-Tak (Corning, 5% (w/v) in acetic acid ...
-
No products found
because this supplier's products are not listed.
Anna V. Protasio, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Worms were rinsed in PBS (3×10mins, 3 × 1hr) and equilibriated in mounting media with 4’,6-diamidino-2-phenylindole DAPI (Fluoromount G, Southern Biotech, Birmingham, AL) overnight before mounting and imaging.
-
No products found
because this supplier's products are not listed.
Icaro Putinhon Caruso, et al.,
bioRxiv - Biophysics 2021
Quote:
... Samples of 20 μΜ N-NTD or N-NTD-SR consisting of varied protein:nucleic acid molar ratios (8:1, 4:1, 2:1, 1:1, 1:2) were prepared in low-binding microtubes (Eppendorf® LoBind) in the presence of 10% PEG-4000 (w/v ...
-
No products found
because this supplier's products are not listed.
Valencia L. Potter, et al.,
bioRxiv - Cell Biology 2020
Quote:
Eye cups from mice aged 4-8 months (Figures 2–5) or 10 days postnatal (Figures 6, 7, 9) were fixed for five minutes in 1% PFA (Electron Microscopy Science) diluted in 1x PBS ...
-
No products found
because this supplier's products are not listed.
Justin E. Silpe, et al.,
bioRxiv - Microbiology 2021
Quote:
... 40 μg mL−1 5-Bromo-4-Chloro-3-Indolyl-beta-D-Galactosidase (X-gal, Takara Bio), and 500 μM isopropyl beta-D-1-thiogalactopyranoside (IPTG ...
-
No products found
because this supplier's products are not listed.
Yoko Hayashi-Takanaka, et al.,
bioRxiv - Cell Biology 2021
Quote:
... and centrifuged at 40,000 rpm (∼128,000 × g) for 3 h at 4°C using an MLS-5 rotor (Beckman Coulter). Aldolase (158 kDa ...
-
No products found
because this supplier's products are not listed.
Alexia Polissidis, et al.,
bioRxiv - Neuroscience 2021
Quote:
For GBA quantification (n=5 for 4 WPI, n=3 for 8 WPI), the surface function of the Imaris software was used to select only GFP-positive cells by appropriately adjusting the number of voxels as well as the intensity of the GFP channel in the threshold tab ...
-
No products found
because this supplier's products are not listed.
Clément Blot, et al.,
bioRxiv - Microbiology 2023
Quote:
The ROS production by macrophages was measured by chemiluminescence in the presence of 5-amino-2,3-dihydro-1,4-phthalazinedione (luminol) using a thermostatically (37°C) controlled for 1 hr (Envision, PerkinElmer). For nitrite release ...
-
No products found
because this supplier's products are not listed.
Eszter Somogyi, et al.,
bioRxiv - Immunology 2020
Quote:
... the media were refreshed and supplemented with 5 ng/mL IL-7 or 5 ng/mL IL-7 and 4 ng/ml IL-2 (R&D Systems), respectively ...
-
No products found
because this supplier's products are not listed.
Faiza Islam, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 1-benzyl-1,4-dihydro-3-pyridinecarboxamide (BNAH) was purchased from TCI Chemicals ...
-
No products found
because this supplier's products are not listed.
Pappu K. Mandal, et al.,
bioRxiv - Microbiology 2020
Quote:
... 5 g L-1 Yeast extract (Oxoid: LP0021)) solidified with 8 g L-1 gellan gum (MP Biomedicals: 180106). The LB gellan gum (LBGG ...
-
No products found
because this supplier's products are not listed.
Elvin Wagenblast, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Between 1×10^3 – 5×10^4 cells were re-suspended in 20μl Buffer P3 (Lonza) per reaction and quickly added to the Eppendorf tube containing the CRISPR/Cas9 gRNA RNP complex ...
-
No products found
because this supplier's products are not listed.
Yuyang Wang, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... 3,4-Dihydro-2H-pyrano[2,3-b]quinolin-7-yl)-(cis-4-methoxycyclohexyl)-methanone (JNJ 16259685) was purchased from HelloBio (Princeton, NJ, USA). N-[4-Chloro-2-[(1,3-dihydro-1,3-dioxo-2H-isoindol-2-yl)methyl]phenyl]-2-hydroxybenzamide (CPPHA ...
-
No products found
because this supplier's products are not listed.
Theresa K Leslie, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... then were incubated for 10 minutes at 21 °C in 1 μM 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM, Biotium) in PSS ...
-
No products found
because this supplier's products are not listed.
Emily J. Talbot, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 4 µM digitonin) with or without 7 µM 2’,3’-cGAMP (Invivogen). Cells were further washed in PBS and incubated in culture medium until collection ...
-
No products found
because this supplier's products are not listed.
Yoko Fujita-Fujiharu, et al.,
bioRxiv - Microbiology 2024
Quote:
Huh-7 cells (1 × 104 cells) were seeded onto an 8-well slide (Ibidi, Gräfelfing, Germany). Transfection with plasmids expressing VP30-GFP ...
-
No products found
because this supplier's products are not listed.
Yiming Niu, et al.,
bioRxiv - Biophysics 2021
Quote:
... purified protein at a concentration of 6–7 mg/mL was mixed with 2.9 mM Fluorinated Fos-Choline-8 (FFC8) (Anatrace) immediately prior to grid preparation ...
-
No products found
because this supplier's products are not listed.
Elisabeth Stelling, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 1 μM dexamethasone (G-Biosciences), 0.5 mM methylisobutylxanthine ...