-
No products found
because this supplier's products are not listed.
Michele Bortolomeazzi, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... the slides were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Akoya Biosciences) and coverslipped using ProLong Gold antifade mounting media (Thermo Fisher Scientific) ...
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No products found
because this supplier's products are not listed.
Jason Decotter, Philippe Desprès, Gilles Gadea,
bioRxiv - Microbiology 2023
Quote:
... Blue-fluorescent DNA stain 4’,6-diamidino-2-phenylindole (DAPI) was purchased from Euromedex (Souffelweyersheim, France). Lipofectamine 3000 (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Trinovita Andraini, et al.,
bioRxiv - Neuroscience 2021
Quote:
... 3-(2,4-Dichloro-5-methoxyphenyl)-2-sulfanyl-4(3H)-quinazolinone (BML-CM127-0050, Enzo Life Sciences) was injected at 20mg/kg (in 10% DMSO ...
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No products found
because this supplier's products are not listed.
Brian T. Do, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... with a 4:2:1:1 ratio of the target:pMDLg:pMD2.G:pRSV-REV plasmids using Transit 293T reagent (Mirus). After 48 hours ...
-
No products found
because this supplier's products are not listed.
Georgina Gyarmati, et al.,
bioRxiv - Physiology 2021
Quote:
... animals underwent brief anesthesia sessions every 3–4 days using 1%–2% isoflurane and the SomnoSuite low-flow anesthesia system (Kent Scientific, Torrington, CT). Body temperature was maintained with a homeothermic blanket system (Harvard Apparatus) ...
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No products found
because this supplier's products are not listed.
Li-Qiang Chen, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and a series of calibrated von Frey filaments (2, 4, 6, 8, 10, 15, 26 g, Stoelting Company, Wood Dale, Illinois, USA) were lightly applied to the skin within the infraorbital territory ...
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No products found
because this supplier's products are not listed.
Bangmin Liu, et al.,
bioRxiv - Cell Biology 2022
Quote:
... the pTr1 cells (2×105 cells/2 ml/well) were seeded in 6-well plates (83.3920; Sarstedt, Nümbrecht, Germany) in CM until the monolayer reached 80-90% confluence ...
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No products found
because this supplier's products are not listed.
Alec Palmiotti, et al.,
bioRxiv - Genetics 2023
Quote:
... we extracted DNA from 2–4 dpf whole larvae using the Quick-DNA Microprep Kit (D3021, Zymo Research). We then performed a PCR for GFP as well as a ‘junction PCR’ to amplify a region including both mitfa and GFP to ensure integration at the intended locus.
-
No products found
because this supplier's products are not listed.
Stéphanie Thébault, et al.,
bioRxiv - Microbiology 2022
Quote:
... Biotinylated SARS-CoV-2 RBD or S1 (4 µg/mL) were loaded on streptavidin SA (18-0009) biosensors (Pall ForteBio) for 1 min ...
-
No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... IP of total SUMOylated protein fractions used either SUMO-1 or SUMO-2/3 Ab included with the Signal Seeker SUMOylation 1 or 2/3 Detection Kit (Cytoskeleton) and IPs were carried out according to the manufacturer instructions ...
-
No products found
because this supplier's products are not listed.
Tim Nierhaus, et al.,
bioRxiv - Microbiology 2021
Quote:
... pooled and concentrated to 2-3 mg/ml using Vivaspin 15R concentrators (2 kDa MWCO HY, Sartorius). Small aliquots were flash-frozen in liquid nitrogen and stored at −80°C ...
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No products found
because this supplier's products are not listed.
Silian Chen, et al.,
bioRxiv - Biochemistry 2020
Quote:
... PBS containing 2 μg/mL Hoechst 33342 and 4 μg/mL pyronin Y (Amresco, cat# 0207) was added to the cells to stain DNA and RNA ...
-
No products found
because this supplier's products are not listed.
Carly R. Mickelson, et al.,
bioRxiv - Neuroscience 2022
Quote:
... mechanically ventilated (2-4 mL tidal volume; VentElite Small Animal Ventilator, Harvard Apparatus, Holliston, MA, USA), and bilaterally vagotomized to prevent mechanosensory feedback from ventilation ...
-
No products found
because this supplier's products are not listed.
Marta Varela-Eirín, et al.,
bioRxiv - Cell Biology 2019
Quote:
... and 1 ng/ml recombinant human fibroblast growth factor-2 (rhFGF-2; Immunotools) or in MesenPRO RS™ Medium supplemented with 100 U/ml penicillin and 100 μg/ml streptomycin.
-
No products found
because this supplier's products are not listed.
Lucas Busta, et al.,
bioRxiv - Plant Biology 2024
Quote:
... (v/v 2:1) with 10 μM 13C(6)-tyrosine (Cambridge Isotope, CLM-1542-0.25) as an internal recovery standard ...
-
No products found
because this supplier's products are not listed.
Judit Serrat, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Blots were blocked for 1 hour at room temperature with 2% BSA diluted in PBST and incubated overnight at 4 °C with 25 μg/ml of PLG (Origene) diluted in blocking solution ...
-
No products found
because this supplier's products are not listed.
Helmut Bischof, et al.,
bioRxiv - Cancer Biology 2023
Quote:
Glucose uptake was assessed using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2- NBDG) (Biomol GmbH). Therefore ...
-
No products found
because this supplier's products are not listed.
Stephen J. DeCamp, et al.,
bioRxiv - Biophysics 2020
Quote:
Polymerized polyacrylamide gels were first treated with 440 μL of a 1:50 (v/v) mixture of Sulfosuccinimidyl 6-(4'-azido-2'-nitrophenylamino)hexanoate (Sulfo-SANPAH) (ProteoChem) and 50 mM HEPES solution under a UV light for 10 minutes ...
-
No products found
because this supplier's products are not listed.
Méghane Sittewelle, Stephen J. Royle,
bioRxiv - Cell Biology 2023
Quote:
... 6 mM of 2-deoxyglucose (Apexbio) and 10 mM of sodium azide (G-Biosciences ...
-
No products found
because this supplier's products are not listed.
Martineau Éric, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Muscles were incubated overnight at 4°C with a rat IgG2a anti-MAC-2 (Galectin-3) antibody (1:250; clone M3/38; Cedarlane, cat# CL8942AP), then 2h at RT with a rabbit IgG anti-S100β antibody (1:250 ...
-
No products found
because this supplier's products are not listed.
Justin M. Westerfield, et al.,
bioRxiv - Biophysics 2021
Quote:
... the peptides were labeled at their N-terminus with an amine-reactive environmentally sensitive fluorescent reporter, Succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoate (NBD-X, SE) (AnaSpec, Fremont, CA) [37] ...
-
No products found
because this supplier's products are not listed.
Mohit Rajabhoj, et al.,
bioRxiv - Plant Biology 2023
Quote:
... from 2 to 3-day-old WT and mea-1-/-;dme-2-/- seedlings using NucleoSpin® Plant II (MACHEREY-NAGEL). 200 ng of DNA was subjected to fragmentation using ultrasonicator (Covaris ...
-
No products found
because this supplier's products are not listed.
Lena H. Nguyen, et al.,
bioRxiv - Neuroscience 2021
Quote:
... p-4E-BP1/2/3-Thr45 (Bioss Antibodies bs-6421R, 1:200, 1:500), anti-HCN4 (Alomone Labs APC-052 ...
-
No products found
because this supplier's products are not listed.
Sherine E. Thomas, et al.,
bioRxiv - Biochemistry 2019
Quote:
... 1–2 M Ammonium sulfate or in sparse matrix screens: Wizard 1&2 (Molecular Dimensions), Wizard 3&4 (Molecular Dimensions) ...
-
No products found
because this supplier's products are not listed.
Natalie Burchat, et al.,
bioRxiv - Molecular Biology 2024
Quote:
Plasma glucagon-like peptide-1 and -2 (GLP-1 and GLP-2) were measured by ELISA (RayBiotech, Peachtree Corners ...
-
No products found
because this supplier's products are not listed.
Ami Vadgama, et al.,
bioRxiv - Immunology 2023
Quote:
... 3-100μM protease-activated receptor 4 agonist (PAR-4; Cambridge Biosciences); 0.03-3μM collagen-related peptide (CRP-XL ...
-
No products found
because this supplier's products are not listed.
Hailee R. Perrett, et al.,
bioRxiv - Immunology 2022
Quote:
... in TSM binding buffer at 4°C for 1 h before StrepMAB-Classic Oyster 645 conjugate (0.5 μg/mL, IBA Lifesciences 2-1555-050) was added ...
-
No products found
because this supplier's products are not listed.
Charles H. Danan, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and run on NuPAGE 4-12% Bis-Tris gels (Thermo NP0335BOX) in 1X MOPS-SDS buffer (BioWorld 10530007-2) or 1X MES-SDS buffer (Thermo NP0002 ...
-
No products found
because this supplier's products are not listed.
Lior Artzi, et al.,
bioRxiv - Microbiology 2021
Quote:
... 100 μL of heat-activated spores were then added and the OD600 was monitored every 2 min for 4 h using Infinite M Plex plate reader (Tecan). The 96-well plate was maintained at 37° C with agitation between measurements ...
-
No products found
because this supplier's products are not listed.
Alexandra A.M. Fischer, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... ACC 635) and U2OS 2-6-3[67] cells were cultivated in Dulbecco’s modified Eagle’s medium (DMEM, PAN Biotech, catalog no. P04-03550) completed with 10% (v/v ...
-
No products found
because this supplier's products are not listed.
Linlin You, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... coli TTC-pause at 13 mg/mL was incubated with 3-([3-cholamidopropyl] dimethylammonio)-2-hydroxy-1-propanesulfonate (CHAPSO, 8 mM, final concentration; Hampton Research Inc.) prior to grid preparation ...
-
No products found
because this supplier's products are not listed.
C Cintas, et al.,
bioRxiv - Cancer Biology 2021
Quote:
Human pancreatic cell lines (Capan-1, BxPC-3, PANC-1, MIA PaCa-2) came from American Type Culture Collection (ATCC), human acute myeloid leukemia cell line (MOLM4 ...
-
2'3'-cyclic GAMP ( cGAMP ) FRET Detection / assay Kit
Cat# K081-F1,
1.0 ea, USD $390.0
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Lisa Mohr, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 4°C for 10 min and 2′3′-cGAMP levels were quantified using the 2′3′-cGAMP ELISA Kit (Arbor Assays) according to the manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Caleb R. Carr, et al.,
bioRxiv - Microbiology 2024
Quote:
... 2 µL of 10 µM 3’ PacBio round 2 reverse primer (PacBio_3pri_RND2), and 25 µL KOD Hot Start Master Mix ...
-
No products found
because this supplier's products are not listed.
Iliodora V. Pop, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Mice (1-2 months old) were injected with 4% (w/v) FG solution in saline (Fluorochrome). Mice were anesthetized with isoflurane and the area above and around the cerebellar region was prepared for surgery ...
-
No products found
because this supplier's products are not listed.
Zheng Shi, Sarah Innes-Gold, Adam E. Cohen,
bioRxiv - Neuroscience 2022
Quote:
... Tethers were pulled with a 4 µm diameter polystyrene bead (Spherotech #DIGP-40-2) held at the tip of a micropipette and controlled by micromanipulators ...
-
No products found
because this supplier's products are not listed.
Romina Ulloa, et al.,
bioRxiv - Cell Biology 2021
Quote:
... ∼2 × 107 3-μm latex NH2-beads (Polyscience) were activated with 8% glutaraldehyde for 4 h at room temperature ...
-
No products found
because this supplier's products are not listed.
Görkem Garipler, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... and 2-inhibitor cocktail (3 mM CHIR (BioVision) and 1 mM PD0325901 (Sigma) ...
-
No products found
because this supplier's products are not listed.
Kevin C. Allan, et al.,
bioRxiv - Developmental Biology 2022
Quote:
96-well plates with parallel-aligned 2-4 μm electrospun fibers (AMS.TECL-005-8X, AMSBio) were washed with 70% ethanol followed by coating with PO for at least 1 hour at 37°C and laminin for at least 3 hours at 37°C ...
-
No products found
because this supplier's products are not listed.
Camilla Schinner, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The following primary antibodies were incubated in antibody buffer (Intercept blocking buffer diluted 1:1 in TBS containing 0.2 % tween 20) at 4 °C overnight: Mouse anti-DSG1/2 (61002, Progen, Heidelberg, Germany), mouse anti-DSP (61003 ...
-
No products found
because this supplier's products are not listed.
Zijun Sun, Thomas C. Südhof,
bioRxiv - Neuroscience 2020
Quote:
... fetal bovine serum (ATLANTA Biological; final concentration = 2-3%) were added to the culture medium ...
-
No products found
because this supplier's products are not listed.
Janne J. Mäkinen, et al.,
bioRxiv - Biochemistry 2020
Quote:
... 2’dUTP and 2’dCTP were from Bioline Reagents (London ...
-
No products found
because this supplier's products are not listed.
Dorothea O Tilley, et al.,
bioRxiv - Immunology 2021
Quote:
Immunising and screening peptides are outlined in S.Table 2 (Figure 3-figure supplement 2) and were synthesized by Eurogentec (Belgium). A portion was further conjugated to Key Lymphocyte Haemoglutinin (KLH ...
-
No products found
because this supplier's products are not listed.
Sheng Zhang, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Axin-2(#6163, RRID: AB_10904353, 1:1000, Prosci) and β-actin (3700 ...
-
No products found
because this supplier's products are not listed.
Ida Paciello, et al.,
bioRxiv - Immunology 2024
Quote:
... 3 µg ml-1 of SARS-CoV-2 subunits diluted in carbonate-bicarbonate buffer (E107, Bethyl Laboratories), were coated in 384-well plates (microplate clear ...
-
No products found
because this supplier's products are not listed.
Matthew S.J. Mangan, et al.,
bioRxiv - Immunology 2021
Quote:
... Rac1/2/3 (1:1000, rabbit polyclonal #2465) from CST, actin (mouse or rabbit, both 1:1,000 dilution) from LI-COR Biosciences ...
-
No products found
because this supplier's products are not listed.
Kangcheng Song, et al.,
bioRxiv - Biophysics 2020
Quote:
... culture medium were discarded and cells were incubated with 50 μl per well of 4 μM Rhod-2/AM (AAT Bioquest) in TARODES’s buffer (10 mM HEPES ...
-
No products found
because this supplier's products are not listed.
Julie Van Coillie, et al.,
bioRxiv - Immunology 2022
Quote:
... 96-well ELISA plates were coated alternatively with 4 µg/ml of SARS-CoV-2-S1 (ACROBiosystems, Newark, DE 19711, USA; #S1N-C52H3) or -S2 (ACROBiosystems ...
-
No products found
because this supplier's products are not listed.
Kritika Khanna, et al.,
bioRxiv - Microbiology 2021
Quote:
... or BHK-21/WI-2 (Kerafast) cells were transfected with SARS-CoV-2 Spike expression plasmid (pTT5 SARS-CoV-2 SΔ21) ...
-
No products found
because this supplier's products are not listed.
Victor Olariu, et al.,
bioRxiv - Systems Biology 2019
Quote:
... One day before the start of co-culture and imaging these micro meshes were adhered to 2-4 (macro)wells of a 24-well glass-bottom plate (P24G-1.0-13-F) (MatTek, Ashland, CA), sterilized with ethanol ...