-
No products found
because this supplier's products are not listed.
Michael Plank, et al.,
bioRxiv - Systems Biology 2022
Quote:
... Cultures were treated with the indicated amount of 1-(1,1-dimethylethyl)-3-(1-naphthalenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine (1-NM-PP1; Millipore Sigma), taken from a 10,000x stock in DMSO ...
-
No products found
because this supplier's products are not listed.
Rahul Bhattacharjee, et al.,
bioRxiv - Cell Biology 2022
Quote:
... methyl]-1-(1,1-dimethylethyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine 4-amino-1-tert-butyl-3-(3-bromobenzyl)pyrazolo[3,4-d]pyrimidine (3BrB-PP1) (Abcam; ab143756) for 30 min ...
-
No products found
because this supplier's products are not listed.
Rafiquel Sarker, et al.,
bioRxiv - Physiology 2022
Quote:
... BPTU (1-(2-(2-(tert-butyl)phenoxy)pyridin-3-yl)-3-(4-(trifluoromethoxy) phenyl) urea was from Tocris Bioscience.
-
No products found
because this supplier's products are not listed.
Blessy Paul, et al.,
bioRxiv - Cell Biology 2024
Quote:
... 3) Oxidative stress induction using 70 μM tert-Butyl hydroperoxide (Thermo Fisher 180345000) for 16-18 h ...
-
No products found
because this supplier's products are not listed.
Amoldeep S. Kainth, Hesheng Zhang, David S. Gross,
bioRxiv - Genetics 2024
Quote:
... then 1-NM-PP1 (4-amino-1-tert-butyl-3-(1’-naphthylmethyl)pyrazolo[3-4-d]pyrimidine) (Toronto Research Chemicals, Inc.; no. A603003) was added to a final concentration of 15 μM ...
-
No products found
because this supplier's products are not listed.
Rahul Bhattacharjee, et al.,
bioRxiv - Cell Biology 2022
Quote:
... cells were grown in YES at 32°C to mid-log phase and treated with 4-amino-1-tert-butyl-3-(3-methylbenzyl)pyrazolo[3,4-3]pyrimidine (3MB-PP1) (Toronto Research Chemical; A602960 or Cayman Chemical; 17860) at a final concentration of 15 µM for 30 min ...
-
No products found
because this supplier's products are not listed.
Dimitra Sakoula, et al.,
bioRxiv - Microbiology 2021
Quote:
... 1.25 μl of a 100 mM Tris(3-hydroxypropyltriazolylmethyl)amine solution (THPTA; 95% purity, Merck KGaA) and 0.3 μl of 5 mM Azide-Fluor 488 in DMSO (≥90% purity ...
-
No products found
because this supplier's products are not listed.
Lina Hacker, et al.,
bioRxiv - Bioengineering 2020
Quote:
DNA was isolated from liver samples of female C57BL/6J mice (3-4 months; n=3) and Wistar rats (6-9 months; n=3) (Charles River) using the Qiagen DNeasy Blood/Tissue kit following the manufacturer’s instruction ...
-
No products found
because this supplier's products are not listed.
Tzu-Yu Feng, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... anti-PDGF-B (F-3, Santa Cruz), anti-GAPDH antibody (6C5 ...
-
No products found
because this supplier's products are not listed.
Melissa Hale, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 5 μg of lysate was heat denatured for 3 minutes at 95°C for 3 minutes and resolved on a pre-cast 4-15 % SDS-PAGE gels (Bio-Rad) at 200V for 45 minutes in 1X running buffer (25mM Tris pH 8.3 ...
-
No products found
because this supplier's products are not listed.
Christopher Douglas, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... HGF (5’-CTCACACCCGCTGGGAGTAC-3’, 5’-TCCTTGACCTTGGATGCATTC-3’), STAT3 (5’-CTTTGAGACCGAGGTGTATCACC-3’, 5’-GGTCAGCATGTTGTACCACAGG-3’) and B-Actin Primer Set (Qiagen, QT00095431). After preparing master mixes samples were prepared in quadruplicate in a 96-well Reaction Microplates (Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Jie Luo, Jeff Ranish,
bioRxiv - Systems Biology 2024
Quote:
Di-tert-butyl 3,3’-Iminodipropionate was purchased from TCI America (Portland ...
-
No products found
because this supplier's products are not listed.
Sapir Herchcovici Levi, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... 3 µM CHIR99021 (PeproTech, SM-2520691-B) and 0.2 µM PD0325901 (PeproTech ...
-
No products found
because this supplier's products are not listed.
M.T. Jahn, et al.,
bioRxiv - Microbiology 2019
Quote:
... were transfected with 20 ng pNF-□B-Luc and 3 ng pRL-TK using FuGENE 6 (Roche). Transfected cells were incubated for 24 h (37°C ...
-
No products found
because this supplier's products are not listed.
Bidisha Mitra, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... For the Caspase-Glo 3/6 Assay (Promega #G8092), Caspase-Glo 3/7 reagent was added to the cells ...
-
No products found
because this supplier's products are not listed.
Sachithrani U. Madugalle, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The original CIRTs-6: B-defensin 3-TBP6.7-YTHDF2 plasmid (# 132544) was purchased from Addgene and the YTHDF2 domain was PCR amplified and cloned into the AgeI and NheI sites ...
-
No products found
because this supplier's products are not listed.
Travis J Gates, et al.,
bioRxiv - Microbiology 2022
Quote:
3–4-week-old female C57BL/6 and BALB/c mice purchased from Jackson Laboratory were used in this study ...
-
No products found
because this supplier's products are not listed.
Ffion R Hammond, et al.,
bioRxiv - Immunology 2023
Quote:
... 1μl Cas9 (diluted 1:3 in diluent B, NEB), 1μl water (water was replaced with 100ng/μl trib1 RNA for cop1 experiments) ...
-
No products found
because this supplier's products are not listed.
N. Sumru Bayin, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Image stacks were obtained every 3-4 minutes for 6-8 hours using a LSM880 (Leica) with an environmental chamber (37°C with 5% CO2) ...
-
No products found
because this supplier's products are not listed.
Juan Liu, et al.,
bioRxiv - Biochemistry 2019
Quote:
... HPLC grade methyl tert-butyl ether was obtained from Alfa Aesar. HPLC grade acetic acid was obtained from Millipore.
-
No products found
because this supplier's products are not listed.
Juan Qin, et al.,
bioRxiv - Biochemistry 2021
Quote:
... PKAc was immobilized via standard N-hydroxysuccinimide (NHS) / 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) amine coupling on a CM5 (carboxyl methyl dextran) sensor chip (GE Healthcare). Before covalent immobilization of PKAc ...
-
No products found
because this supplier's products are not listed.
Pinanong Na-Phatthalung, et al.,
bioRxiv - Immunology 2023
Quote:
... The nuclei were stained with 3 µM DAPI (4’,6-diamidino-2-phenylindole; BioLegend, #422801) for 5 min ...
-
No products found
because this supplier's products are not listed.
Yiming Fang, et al.,
bioRxiv - Cancer Biology 2023
Quote:
3×105 OVCAR3 cells were seeded with 3×105 CAFs in 6-well ultra-low attachment plates (Corning). At the time of seeding ...
-
No products found
because this supplier's products are not listed.
Handi Cao, et al.,
bioRxiv - Immunology 2022
Quote:
... for 3 days and Stemdiff B (STEMdiff™ Hematopoietic Kit, Stem cell technologies) for 7 days as described previously (41) ...
-
No products found
because this supplier's products are not listed.
Phuoc H. T. Ngo, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... ribosome was purified using hydrophobic chromatography (3×5 mL HiTrap Butyl HP column (VWR)) with Fast Performance Liquid Chromatography (FPLC ...
-
No products found
because this supplier's products are not listed.
Kun Wang, et al.,
bioRxiv - Biochemistry 2022
Quote:
... lipid contents were extracted using the tert-butyl-methyl ether and methanol method47 from purified MBOAT7 proteins and lipid controls (Avanti Polar Lipids). Extracted lipids were resolubilized in 300 μL of isopropanol ...
-
No products found
because this supplier's products are not listed.
Ellen Pun Casavant, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... 95-3% 6.5 minutes) using an Agilent 1200 G1312 Binary pump (Agilent Technologies, San Jose, CA). The column was coupled to a home-built electrospray ionization source and LTQ-Velos Orbitrap mass spectrometer (Thermo Scientific ...
-
No products found
because this supplier's products are not listed.
Katja Ester, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and 3 μM Fluo-4 AM dye (BD Biocsiences). After 60 min incubation in the dark at 37 °C ...
-
No products found
because this supplier's products are not listed.
Caitlyn B. Smith, et al.,
bioRxiv - Microbiology 2024
Quote:
... zonula occludens-3 (ZO-3) (Cell Signaling Technologies), anti-mouse IgG (Alexa Fluor® 488 ...
-
No products found
because this supplier's products are not listed.
James A. D’Amour, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Electrodes of 4-6 MOhm resistance (borosilicate glass, World Precision Instruments, no. TW150F-3) were prepared on Narishige (PP-830 ...
-
No products found
because this supplier's products are not listed.
Vignesh Venkatakrishnan, et al.,
bioRxiv - Biochemistry 2020
Quote:
... disrupted with a tip sonicator at 1/3 power (8 MHz) for 3 × 30 seconds on ice and fractionated by ultracentrifugation (100’000 x g, 1H, Beckman TLA 120.2 rotor). The luminal fraction was concentrated to around 50 µL on a 3 kDa MWCO Amicon Ultra 0.5 mL centrifugal filter while membrane pellets were dissolved in 50 µL TBS with 2% SDS ...
-
No products found
because this supplier's products are not listed.
Caitriona M. McEvoy, et al.,
bioRxiv - Immunology 2021
Quote:
Commercially available human primary PTs from 6 donors (3 males and 3 females, Lonza Walkersville Inc) were expanded at passage 4 ...
-
No products found
because this supplier's products are not listed.
Matthew Denholtz, et al.,
bioRxiv - Genomics 2019
Quote:
... cells were washed 3×3 minute in PBS and fixed for 30 minutes in 6% paraformaldehyde (Electron Microscopy Sciences 15710) in 1x PBS ...
-
No products found
because this supplier's products are not listed.
Natasja L. de Vries, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 3 µg/mL anti-ULBP2/5/6 (clone 165903, R&D Systems), and 6 µg/mL anti-ULBP3 (clone 166510 ...
-
No products found
because this supplier's products are not listed.
Evelyne Krin, et al.,
bioRxiv - Microbiology 2023
Quote:
... 3-(2,4-dinitrostyryl)-(6 R,7 R)-7-(2-thienylacetamido)-ceph-3-em-4-carboxyl acid (Calbiochem), was used to assess membrane permeability ...
-
No products found
because this supplier's products are not listed.
Andrew Pountain, et al.,
bioRxiv - Systems Biology 2023
Quote:
... All oligos were synthesized with a 3’ amine modification (LGC Biosearch Technologies). The oligos against a given gene (oligo set ...
-
No products found
because this supplier's products are not listed.
T Winogrodzki, et al.,
bioRxiv - Bioengineering 2022
Quote:
... (3) LPS and 150 μg/ml Polymyxin B (Invivogen) for 45 and 90 min ...
-
No products found
because this supplier's products are not listed.
Anjali Verma, et al.,
bioRxiv - Plant Biology 2023
Quote:
... The primers were annealed at 95°C for 4 min followed by 70°C for 3 min in a thermocycler (Eppendorf, Hamburg, Germany). The reaction was allowed to cool slowly to room temperature ...
-
No products found
because this supplier's products are not listed.
Antonios Apostolopoulos, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... for 1[h at 60°C and was subsequently PCR amplified using the primers 5′-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTC-3′ and 5′-CAAGCAGAAGACGGCATACGAGATJJJJJJGTGACTGGAGTTCAGACGTGTG-3′(where Js indicates the 6-mer index sequence for Illumina sequencing).
-
No products found
because this supplier's products are not listed.
Korrie L. Mack, et al.,
bioRxiv - Bioengineering 2020
Quote:
... Cells were washed 3 times with cold PBS and resuspended in Vectashield mounting medium with 4’,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories). Cells were imaged at 100X magnification using a Leica DM IRBE microscope ...
-
No products found
because this supplier's products are not listed.
Vadim Molodtsov, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... ≥95% purity for 3’-dGTP (Jena Bioscience)] and incubated 2 min at 37°C (to “chase,” and thereby to stabilize ...
-
No products found
because this supplier's products are not listed.
Gang Ye, et al.,
bioRxiv - Microbiology 2020
Quote:
Male C57BL/6 mice (3 to 4 weeks old) (Envigo) were intravenously injected (tail-vein ...
-
No products found
because this supplier's products are not listed.
Yuishin Kosaka, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... The deletion of the lacZ gene was verified using blue-white selection on an LB agar plate containing 0.1 mM isopropyl-β-D-thiogalactopyranoside (Nacalai Tesque) and 4.0 × 10−3 % 5-bromo-4-chloro-3-indolyl-β-D-galactoside (Takara Bio).
-
No products found
because this supplier's products are not listed.
Jelena Platisa, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Pipettes were pulled from thin-walled borosilicate capillaries without filament (3-6 MΩ) (Sutter Instruments), and filled with internal solution containing (in mM ...
-
No products found
because this supplier's products are not listed.
Lamuk Zaveri, Jyotsna Dhawan,
bioRxiv - Cell Biology 2021
Quote:
... Klf4 or Oct-3/4 or Klf4 + Oct-3/4 or Klf4 + OctER using PEI (Polysciences, USA). Self-ligated empty pGEM-T vector (Promega ...
-
No products found
because this supplier's products are not listed.
Johanna Theruvath, et al.,
bioRxiv - Immunology 2021
Quote:
... 4 minutes after 3 mg d-luciferin (PerkinElmer) was injected intraperitoneally ...
-
No products found
because this supplier's products are not listed.
Martin Baccino-Calace, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Larvae were dissected and sharp-electrode recordings were made from muscle 6 in abdominal segments 3 and 4 using an Axoclamp 900A amplifier (Molecular Devices). The extracellular HL3 saline contained (in mM) ...
-
No products found
because this supplier's products are not listed.
Valentina Salvi, et al.,
bioRxiv - Immunology 2021
Quote:
... and PE-conjugated anti-IL-4 (clone 7A3-3, Miltenyi Biotec) following the manufacturer’s recommendations ...
-
No products found
because this supplier's products are not listed.
Antonio Marino, et al.,
bioRxiv - Systems Biology 2023
Quote:
... 4 3 2.0 mm SecurityGuard (Phenomenex) cartridge as a guard column ...
-
No products found
because this supplier's products are not listed.
Katrin Gerstmann, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Electrodes (3-6 MΩ, borosilicate glass Harvard Apparatus) were filled with a solution containing (in mM) ...