-
No products found
because this supplier's products are not listed.
Satoshi Watanabe, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 2 and 6 (3M and 6M, mIPSCs), respectively.
-
No products found
because this supplier's products are not listed.
Bao V. Nguyen, et al.,
bioRxiv - Biochemistry 2024
Quote:
Kinase activity was monitored by Synergy H1 microplate reader (BioTek) at 30 °C as previously described with minor modifications (Stratton et al. ...
-
No products found
because this supplier's products are not listed.
Sara M. Blazejewski, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and C and Nuak kinases 1 and 2 were purchased from OriGene Technologies ...
-
No products found
because this supplier's products are not listed.
Cristina Esteva-Font, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... DAPI (4’,6-diamidino-2-phenylindole, MP Biomedicals, Solon, OH) was added at 300 nM for 15 min ...
-
No products found
because this supplier's products are not listed.
Kyung Duk Koh, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... HBECs were seeded on human placental collagen (HPC)-coated 6-well dishes and propagated in BEGM supplemented with the rho-associated protein kinase inhibitor Y-27632 (10 µM; Enzo Life Sciences, Farmingdale, NY), which enhances cell proliferation (16) ...
-
No products found
because this supplier's products are not listed.
Juhi Singh, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 6 mM 2-mercaptoethanol and centrifuged with a 70Ti (Beckman coulter) rotor at 40,000 rpm for 18 hours at 4°C ...
-
No products found
because this supplier's products are not listed.
Daniel Bull, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Calcium 6 dye (Molecular Devices) was diluted in HBSS assay buffer (Gibco ...
-
No products found
because this supplier's products are not listed.
Robert C. Klipp, John R. Bankston,
bioRxiv - Biophysics 2022
Quote:
... pulled to a resistance of 2–6 MΩ (P-1000; Sutter Instrument) and filled with an internal solution containing (in mM) ...
-
No products found
because this supplier's products are not listed.
Étienne Fafard-Couture, et al.,
bioRxiv - Genomics 2021
Quote:
... followed by dephosphorylation using T4 Polynucleotide Kinase (Epicentre) and final purification using ...
-
No products found
because this supplier's products are not listed.
Fatima El Alaoui, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Rhodamine-PE and Laurdan (6-Dodecanoyl-2-dimethylaminonaphthalene) are from Avanti Polar Lipids, Inc ...
-
No products found
because this supplier's products are not listed.
Clara W. Liff, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Samples were permeabilized for 2 days and blocked in 6% goat serum (Jackson ImmunoResearch, 005-000-121) for 2 days at 37°C ...
-
No products found
because this supplier's products are not listed.
Hanna Braun, et al.,
bioRxiv - Cell Biology 2022
Quote:
For esiRNA transfection 35,000 U2OS cells were seeded in 2 ml medium in 6-well plates (Eppendorf) the evening before transfection ...
-
No products found
because this supplier's products are not listed.
Zengqin Deng, et al.,
bioRxiv - Biophysics 2020
Quote:
... 3.5 µl of purified channel proteins at ∼6 mg/ml were pipetted onto glow-discharged copper Quantifoil R2/2 holey carbon grids (Quantifoil). Grids were blotted for 2 s at ∼100% humidity and flash frozen in liquid ethane ...
-
No products found
because this supplier's products are not listed.
Wen-Yu Cheng, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... 6-Diamidino-2-phenylindole (DAPI) and the slides were examined under a laser confocal microscope (Olympus FV-1000, Tokyo, Japan). Images of the cells were analyzed by FV10-ASW 3.0 software (Tokyo ...
-
No products found
because this supplier's products are not listed.
Dongyang Wang, et al.,
bioRxiv - Cell Biology 2022
Quote:
... BMDMs were plated in 6-well plates (2×105 cells/well) and treated with 1 ng/mL LPS and 20 ng/mL mIFNγ (Sino Biological Inc., Beijing, China) for 24 h ...
-
No products found
because this supplier's products are not listed.
Surya Prakash Rao Batta, et al.,
bioRxiv - Cell Biology 2022
Quote:
HUVECs (passage 2 to 6; PromoCell) were routinely cultured in EBM media supplemented with the provided growth factors kit (Promocell) ...
-
No products found
because this supplier's products are not listed.
J. Cody Herron, et al.,
bioRxiv - Systems Biology 2022
Quote:
... supplemented with 2% HI-FBS and 20 μM Rho kinase inhibitor Y-27632 (Hello Bio, HB2297) for 25 min of inhibition during frustrated phagocytosis ...
-
No products found
because this supplier's products are not listed.
Whee-Soo Kim, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 4’,6-diamidino-2-phenylindole (DAPI, TCI Chemicals, Tokyo, Japan) was added to stain the nuclei of the organoids ...
-
No products found
because this supplier's products are not listed.
Sujung Choi, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... -pyruvate dehydrogenase kinase 1 (PDK1, Novus Biologicals, cat # 100-2383), - transcription factor II B (TFIIB ...
-
No products found
because this supplier's products are not listed.
Monika Chodasiewicz, et al.,
bioRxiv - Plant Biology 2022
Quote:
... Thermal unfolding of G-actin (2 μM) and F-actin (2 μM) was performed with the Tycho NT.6 (Nanotemper, Munich, Germany) according to the manufacturer’s instructions in G-buffer (5 mM Tris-HCl ...
-
No products found
because this supplier's products are not listed.
Miranda L. Curtiss, et al.,
bioRxiv - Immunology 2022
Quote:
... in the presence of 10μg/ml rIL-6 (R&D) and neutralizing antibodies to IL-2 (JES6-1A12 BioXcell, 5μg/ml and S4B6-1 BioXcell, 5μg/mL).
-
No products found
because this supplier's products are not listed.
Dmitry Molotkov, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The virus solution (0.4-0.6 µL) was injected at a rate of 2 nL/s with a microinjection pump (either Neurostar NanoW or WPI NanoLiter 2010). The pipette was slowly withdrawn at least 10 minutes after the completion of the injection ...
-
LC Laboratories' Product Number R-1234 - Roscovitine, Free Base (Seliciclib, CYC202,...
Cat# R-1234, SKU# R-1234_10mg,
10 mg, $49.00
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Marisol Romero-Tejeda, et al.,
bioRxiv - Cell Biology 2023
Quote:
... supplemented with 6 µM of glycogen synthase kinase 3-inhibitor CHIR99021 (LC Labs, C-6556). On day 1 ...
-
No products found
because this supplier's products are not listed.
Dong Hun Lee, et al.,
bioRxiv - Physiology 2022
Quote:
... HAPECs (passage 2-6, ScienCell, Carlsbad, CA) were incubated with 0.1 ng/mL interleukin-1 beta (IL-1β) ...
-
No products found
because this supplier's products are not listed.
Chhiring Lama, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... 6-diamidino-2-phenylindole (Spectral DAPI, Akoya Biosciences) was applied per provided protocols to label nuclei ...
-
No products found
because this supplier's products are not listed.
Hengping Xu, et al.,
bioRxiv - Cell Biology 2022
Quote:
Kinase activity was measured using ADPsensor™ Universal Kinase Activity Assay Kit (BioVision). HA-tagged CDKB1 ...
-
No products found
because this supplier's products are not listed.
David Salcedo-Tacuma, et al.,
bioRxiv - Cell Biology 2024
Quote:
... employing 2 mM 6-FAM-dC-Puromycin (Jena Bioscience), according to Wang et al ...
-
No products found
because this supplier's products are not listed.
Sara M. Blazejewski, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and mRFP + Nuak 1 and 2 kinases was performed via chemical transfection using TransIT-X2 (Mirus Bio, Madison, WI) 48 hours following plating ...
-
No products found
because this supplier's products are not listed.
Hugo Belda, et al.,
bioRxiv - Microbiology 2024
Quote:
... 3µl kinase at 40nM in kinase reaction buffer (20mM MOPS, 10mM magnesium chloride and 10mM manganese chloride, pH 7.4, Alfa Aesar) was dispensed in each well using a Multidrop™ Combi Reagent dispenser (ThermoFischer Scientific) ...
-
No products found
because this supplier's products are not listed.
Rodrigo Mohallem, Uma K. Aryal,
bioRxiv - Systems Biology 2020
Quote:
2 mL of collected spent media was loaded in Vivaspin 6™ Centrifugal Concentrator 10,000 MWCO PES (Sartorius AG, Germany) and centrifuged at 4000 RPM for 13 minutes to concentrate samples ...
-
No products found
because this supplier's products are not listed.
Nikita Raj, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 5-6 × 105 siRNA transfected or untransfected cells were cultured on collagen-coated 6-well plates (Ibidi, 80287) until 90-100 % confluency (20-24 hours) ...
-
No products found
because this supplier's products are not listed.
Zhenglin Zhu, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Pyruvate kinase M-2 (PKM2) (ABclonal, A16700), α-SMA (ABclonal ...
-
No products found
because this supplier's products are not listed.
I.R. Akberdin, et al.,
bioRxiv - Systems Biology 2021
Quote:
... Another target of calmodulin is Ca2+/calmodulin-dependent protein kinase kinase 2 (CAMKK2) that phosphorylates AMPK Thr172 thereby activating the kinase (Abbott et al., 2009). In turn ...
-
No products found
because this supplier's products are not listed.
Avishek Ghosh, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 50 µl of 2× kinase buffer containing 80 µM O18 ATP (OLM-7858-PK, Cambridge Isotope laboratories, Inc) and 1 µg of bacterial purified human PIP4Kα-GST was added and the reaction was incubated at 30 °C for 1 hour ...
-
6 well glass bottom plate with high performance #1.5 cover glass (0.170±0.005mm). Black frame,...
Cat# P06-1.5H-N,
20/case, $249.00
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Sara M. Tudor, et al.,
bioRxiv - Cell Biology 2019
Quote:
... 2 mL was cured overnight at 37C in each well of a 6-well glass bottom plate (Cellvis). Using a biopsy punch (cat no ...
-
No products found
because this supplier's products are not listed.
Meraj Ramezani, et al.,
bioRxiv - Cell Biology 2023
Quote:
... then immediately resuspended in 6 ml medium and cultured in MatTek dishes (2 ml/dish) (MatTek Corporation, Ashland, MA) for 24 hr to recover ...
-
No products found
because this supplier's products are not listed.
Paul J Banks, E Clea Warburton, Zafar I Bashir,
bioRxiv - Neuroscience 2020
Quote:
... were targeted under oblique infra-red illumination based on somatic morphology and patch clamped using 2-6 MΩ boroscillicate glass electrodes (GC150-10F, Harvard Apparatus) filled with potassium gluconate internal for current-clamp experiments (in mM ...
-
No products found
because this supplier's products are not listed.
Lucas Serra Moncadas, et al.,
bioRxiv - Microbiology 2023
Quote:
... All processed samples were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) and analyzed by epifluorescence microscopy (Zeiss Imager Z2, Carl Zeiss, Oberkochen, DE) with a colibri LED system and the following filter sets ...
-
Building Block
Sold for research purposes only.
Cat# 1028.0, SKU# 1028-100 mg,
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Bart Smeets, et al.,
bioRxiv - Biophysics 2019
Quote:
... 10 or 20 µM Rho-kinase inhibitor Y-276323 (Axon Medchem).
-
No products found
because this supplier's products are not listed.
Matteo Tassinari, et al.,
bioRxiv - Microbiology 2020
Quote:
... 20 mL of solubilization solution supplemented with the 2 % 6-Cyclohexyl-1-Hexyl-β-D-Maltoside (Cymal6, Anatrace) was added gently to the membrane solution and the sample incubated at RT for 30 min under moderate shaking ...
-
No products found
because this supplier's products are not listed.
Kyra L. Feuer, et al.,
bioRxiv - Genetics 2022
Quote:
RNA was extracted from pellets of ∼2 million iPSCs or ∼6 million neurons immediately after harvesting with the Quick-RNA Miniprep Kit (Zymo R1054) following manufacturer’s instructions except for two alterations ...
-
No products found
because this supplier's products are not listed.
Juliane Mietz, et al.,
bioRxiv - Immunology 2023
Quote:
... Wells were washed and incubated for 2 hours with biotinylated anti-IFN-ψ detection antibody (mAb-7-B6-1-Biotin, Mabtech, Cat. 3420-6-250). Afterwards ...
-
No products found
because this supplier's products are not listed.
Celia Fernandez-Sanz, et al.,
bioRxiv - Physiology 2021
Quote:
... INF-2 (GeneTex; CTX130714), SPIRE-1C (custom made ...
-
No products found
because this supplier's products are not listed.
Muhammed Jamsheer K, et al.,
bioRxiv - Plant Biology 2020
Quote:
... and total (Ribosomal S6 kinase 1/2 antibody; dilution: 1:2000; catalog no.: AS12 1855, Agrisera) level of S6Ks ...
-
No products found
because this supplier's products are not listed.
Jason Neidleman, et al.,
bioRxiv - Immunology 2020
Quote:
... 6×106 cells were resuspended at room temperature in 2 ml PBS (Rockland) with 2 mM EDTA (Corning) ...
-
No products found
because this supplier's products are not listed.
Joel E. Graham, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 6 neoprene stoppers (RPI-259100-6) and capped with media bottle lid with a center bore to access the stopper ...
-
No products found
because this supplier's products are not listed.
Elyse M. Digby, et al.,
bioRxiv - Biophysics 2021
Quote:
4’,6-Diamidino-2-phenylindole 2HCl (purchased from Biosynth Carbosynth, 1 eq., 0.0100 g, 0.029 mmol) was dissolved in 1:1 distilled water/acetone (1 mL) ...
-
No products found
because this supplier's products are not listed.
Axel Soto-Serrano, et al.,
bioRxiv - Genomics 2024
Quote:
DNA concentration was normalized to 50 ng/μl and sheared to 6 Kb fragments using Megaruptor® 2 (Diagenode, Seraing, Belgium). Shearing quality was estimated with TapeStation 4200 (Agilent Technologies ...
-
No products found
because this supplier's products are not listed.
Kaiyi Jiang, et al.,
bioRxiv - Genomics 2022
Quote:
... Hepa 1-6 cells (American Type Culture Collection (ATCC) -CRL-1830) ...
-
No products found
because this supplier's products are not listed.
Meena Kusi, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... 2×150 mm (Phenomenex); mobile phase A ...