-
No products found
because this supplier's products are not listed.
Hiroshi Nishiyama, et al.,
bioRxiv - Neuroscience 2023
Quote:
... the AMPA-R antagonist 2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide (NBQX;10 μM HB0443, Hello Bio) and NMDA-R antagonist DL-2-Amino-5-phosphonopentanoic acid (DL-APV ...
-
No products found
because this supplier's products are not listed.
Yusuke Ohno, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 1H NMR (CDCl3; Cambridge Isotope Laboratories ...
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No products found
because this supplier's products are not listed.
François Halloy, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 5-(Benzylthio)-1H-tetrazole (Carbosynth) was used at 0.24 M concentration in dry acetonitrile to activate the phosphoramidites for coupling ...
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No products found
because this supplier's products are not listed.
Yihe Huang, Wei Lü, Juan Du,
bioRxiv - Biophysics 2023
Quote:
... For nanodisc samples, 0.5 mM (1H, 1H, 2H, 2H-Perfluorooctyl)-β-D- Maltopyranoside (FOM, Anatrace) was added for improving particles distribution and contrast ...
-
No products found
because this supplier's products are not listed.
James A. D’Amour, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Electrodes of 4-6 MOhm resistance (borosilicate glass, World Precision Instruments, no. TW150F-3) were prepared on Narishige (PP-830 ...
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No products found
because this supplier's products are not listed.
Nittay Meroz, et al.,
bioRxiv - Evolutionary Biology 2023
Quote:
... The optical density was then measured using 6 automated plate readers (3 Epoch2 microplate reader - BioTek and 3 Synergy microplate reader - BioTek) simultaneously ...
-
No products found
because this supplier's products are not listed.
Esmeralda Vásquez Pacheco, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 3 × 105 cells were seeded per well in 6-well plates (Greiner Bio-One). The following day ...
-
No products found
because this supplier's products are not listed.
Claire C. Chen, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product by mitochondrial dehydrogenase enzymes ...
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3-Nitro-L-tyrosine (3-NT, N-Tyr, MNT), synthesized from L-tyrosine by peroxynitrite, is a...
Cat# S6345, SKU# S6345-100mg,
100mg, $97.00
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Liying Chen, et al.,
bioRxiv - Neuroscience 2023
Quote:
The D1 receptor antagonist R(+)-7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepi ne hydrochloride (SCH-23390, Selleck chemicals, China) was dissolved in 0.9% saline and stored -80°C ...
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No products found
because this supplier's products are not listed.
Julianna L. Sun, et al.,
bioRxiv - Microbiology 2022
Quote:
... Membranes were developed using 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) (Sigma Aldrich, 11697471001. Images were captured using a Nikon Z1000 microscope.
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No products found
because this supplier's products are not listed.
Fernando R. Valencia, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 7.5 and 10 μM (S)-nitro-blebbistatin (Toronto Research Chemicals), 2 μM Y-27632 ROCK inhibitor (EMD Millipore) ...
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Cat# HY-113248-100 mg,
100 mg, USD $50.0
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Estefania Sanchez-Vasquez, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... 1h treatment with 5uM dimethylenastron (MedChemExpress, HY-19944) and 10uM Mg132 in KSOM ...
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No products found
because this supplier's products are not listed.
Matthias M. Zimmer, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... RNAs were labeled at the 3’ end using pCp-Cy5 (Cytidine-5’-phosphate-3’-(6-aminohexyl) phosphate) (Jena Biosciences). For each binding experiment ...
-
No products found
because this supplier's products are not listed.
Weronika Czaban, Jim Rasmussen,
bioRxiv - Plant Biology 2019
Quote:
... ground plant material was extracted in a 1-ml extraction mixture of chloroform, methanol and water (1:3:1, v:v:v) in Sarstedts Eppendorf tubes (Sarstedt AG & Co, Nümbrecht, Germany). One metal bead (a 3-mm tungsten carbide bead ...
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No products found
because this supplier's products are not listed.
Raisa I. Krutilina, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... with 3 @g of either pCMV-6-Entry vector (OriGene, cat# PS100001, Rockville, MD) or pCMV-6-Entry expressing the CKB TrueORF (cat#RC203669) ...
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No products found
because this supplier's products are not listed.
Mengjun Ma, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Disease was induced between 6 and 10 weeks of age using 3 mg curdlan (InvivoGen) administered by intraperitoneal injection ...
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No products found
because this supplier's products are not listed.
Michelle Reed, et al.,
bioRxiv - Neuroscience 2022
Quote:
... FBF1 (10 min methanol-acetone fix, 1:200, Proteintech). Primary antibodies were diluted in blocking buffer and applied to sections ...
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No products found
because this supplier's products are not listed.
Yoshimi Haga, et al.,
bioRxiv - Biochemistry 2021
Quote:
... the free N-glycan were dissolved in methanol and 0.5 μL of methanol solution was applied onto a Opti-TOF™ 384-well plate (AB Sciex, Foster City, CA) together with 0.5 μL of CHCA matrix solution (4 mg/mL concentration in 70% acetonitrile ...
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No products found
because this supplier's products are not listed.
Ning Tsao, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... the U2OS 2-6-3 cells expressing degron-tagged LacI fusion proteins were incubated with 300 nM Shield1 ligand (Takara Bio) and 1 µg/mL doxycycline for 24 hours ...
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No products found
because this supplier's products are not listed.
Thomas I. R. Hopkins, et al.,
bioRxiv - Biophysics 2021
Quote:
... 100% (3 × 1h)) and then placed in Histoclear (Cat. No HS-200, National Diagnostics) overnight ...
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No products found
because this supplier's products are not listed.
Mattia Storti, et al.,
bioRxiv - Plant Biology 2020
Quote:
... proteins were transferred onto nitro-cellulose membrane (Pall Corporation) and detected with specific commercial (anti-PsaD ...
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6 well glass bottom plate with high performance #1.5 cover glass (0.170±0.005mm). Black frame,...
Cat# P06-1.5H-N,
20/case, $249.00
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R. A. Petazzi, et al.,
bioRxiv - Biophysics 2020
Quote:
... 3-6 · 105 cells were plated onto 35 mm glass-bottom dishes (CellVis, Mountain View ...
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No products found
because this supplier's products are not listed.
Zhibin Zhang, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Frizzled-3 (FZD3; Uniprot ID: Q9NPG1) and Frizzled-6 (FZD6; Uniprot ID: O60353) were synthesized by GenScript. For FZD1 ...
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No products found
because this supplier's products are not listed.
Emily G. Kuiper, et al.,
bioRxiv - Biochemistry 2019
Quote:
... was monitored during unfolding over a linear temperature gradient (35-95 °C) over 3 minutes on a Tycho NT.6 instrument (NanoTemper). The temperature at which a transition occurs in a plot of the first derivative of the fluorescence ratio (350/330 nm) ...
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No products found
because this supplier's products are not listed.
Samantha Sarni, et al.,
bioRxiv - Biochemistry 2020
Quote:
... The following day they were transfected with 6 μg Gag plasmid + 6 μg vector plasmid + 3 μg pCMV-Rev (to support nuclear export of vector RNA) using Transit-293 (Mirus) following the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Stephen Abini-Agbomson, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Cells were incubated in blocking solution (3% BSA in TBS-Tween 0.1%) for 1h with primary antibody (anti-53BP1, Rabbit polyclonal, Bethyl #A300-272A) diluted in blocking solution overnight at 4°C ...
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No products found
because this supplier's products are not listed.
Coline Arone, et al.,
bioRxiv - Microbiology 2023
Quote:
... using wet transfer with tris-glycine-methanol buffer (milli-Q H2O supplemented with 15% methanol and 10% 10X Tris-glycine solution, Euromedex). Then ...
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No products found
because this supplier's products are not listed.
Anna V. Protasio, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Worms were rinsed in PBS (3×10mins, 3 × 1hr) and equilibriated in mounting media with 4’,6-diamidino-2-phenylindole DAPI (Fluoromount G, Southern Biotech, Birmingham, AL) overnight before mounting and imaging.
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No products found
because this supplier's products are not listed.
Agata Szuba, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 5 mM dithiothreitol (DTT)) at 4°C overnight and concentrated to ~3-5 mg/mL using Vivaspin 6 concentrators (Sartorius, 6 ml, 100 kDa cut-off). Mammalian septin hexamers composed of mouse Sept2 (98.6% identical to human Sept2 ...
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Prepared to contain high collagenase activity with a caseinase to collagenase ratio of ~2:1....
Cat# LS005318,
100 mg, $60.00
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Miqdad O. Dhariwala, et al.,
bioRxiv - Immunology 2020
Quote:
... and skin was minced finely with dissection scissors and mixed in a 6-well plate with 3 ml of digestion buffer consisting of 0.8 mg/ml Collagenase Type 4 (4188; Worthington), 0.02 mg/ml DNAse (DN25-1G ...
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No products found
because this supplier's products are not listed.
Pablo Barbeito, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Immunoprecipitates were then digested for 1h at 25°C with TEV protease (BioVision, #7847) in a buffer containing 50 mM Tris-HCl ...
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No products found
because this supplier's products are not listed.
R. A. Petazzi, et al.,
bioRxiv - Biophysics 2020
Quote:
... 3-6 · 105 cells were plated onto 35 mm glass-bottom dishes (CellVis, Mountain View, CA or MatTek Corp., Ashland, MA) 48 h prior to the experiment and transfected as described above ...
-
No products found
because this supplier's products are not listed.
Fynn M. Hansen, et al.,
bioRxiv - Systems Biology 2020
Quote:
... samples were four-fold diluted with 1% TFA in isopropanol and loaded onto SDB-RPS cartridges (Strata™-X-C, 30 mg/ 3 ml or Strata™-X-C, 200 mg/ 6 ml, Phenomenex Inc.). Before peptide loading ...
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No products found
because this supplier's products are not listed.
Danielle M Paul, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Kinesore (3,5-dibromo-N′-[2,5-dimethyl-1-(3-nitrophenyl)-1H-pyrrol-3-yl]methylene}-4-hydroxybenzohydrazide) was obtained from Chembridge Corporation (Cat ...
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No products found
because this supplier's products are not listed.
C. Jenul, et al.,
bioRxiv - Microbiology 2023
Quote:
... hexakis (1H,1H,2H-difluoroethoxy)phosphazene ions (Apollo Scientific, m/z 622.1978) located on a wick within the source was used ...
-
No products found
because this supplier's products are not listed.
Subash Godar, et al.,
bioRxiv - Biophysics 2021
Quote:
... We detected the bound alkaline phosphatase labeled antibodies by incubating the membrane in BCIP/NBT (5-bromo, 4-chloro, 3- indolylphosphate/nitro-blue tetrazolium, AMRESCO, LLC.) substrate for 30–45 minutes ...
-
No products found
because this supplier's products are not listed.
Jossana A. Damasco, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Sulfo-Cy5.5-NHS ester (potassium 3-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-1,1-dimethyl-2-((1E,3E,5E)-5-(1,1,3-trimethyl-6,8-disulfonato-1,3-dihydro-2H-benzo[e]indol-2-ylidene)penta-1,3-dien-1-yl)-1H-benzo[e]indol-3-ium-6,8-disulfonate) was obtained from Lumiprobe (Hunt Valley, MD, USA).
-
No products found
because this supplier's products are not listed.
Lin Di, et al.,
bioRxiv - Genomics 2022
Quote:
... at 37°C for 1h and purified by Zymo columns ...
-
No products found
because this supplier's products are not listed.
Seraphine Kamayirese, et al.,
bioRxiv - Biochemistry 2023
Quote:
(His)6-14-3-3ε was commercially obtained from Novus Biologicals (Centennial CO, USA), and 14-3-3ε-(His)12 was expressed in our laboratory (see details on protein expression in the supporting information) ...
-
No products found
because this supplier's products are not listed.
Si Chen, et al.,
bioRxiv - Neuroscience 2021
Quote:
... with the addition of an antigen retrieval step consisting of 100% methanol incubation at −20 C for 30 minutes for staining pSTAT3 (MBL International Corporation, D128-3). For retinal flat-mounts ...
-
No products found
because this supplier's products are not listed.
Dongdong Chen, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Mycoplasma testing was performed every 3-6 months using the Mycoplasma test kit (PromoCell, PK-CA91-1024) and on an ad hoc basis.
-
No products found
because this supplier's products are not listed.
P.A. Kipnis, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Membranes were blocked in Rockland Blocking Buffer for 1h (Rockland #MB-070, USA). Membranes underwent 6h incubation with primary antibodies ...
-
No products found
because this supplier's products are not listed.
Zixiang Wang, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Colonies were fixed with methanol and stained with 0.1% crystal violet (Solarbio), and the number of colonies was counted with Image J ...
-
No products found
because this supplier's products are not listed.
Koetsu Inoue, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and anti-mouse PD-1 antibodies (clone RMP1-14, 10 mg/kg, 6 doses, every 3 days) were purchased from BioXcell. All drug treatments were administered intraperitoneally (i.p.).
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No products found
because this supplier's products are not listed.
Luis M. Rodríguez-Pérez, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The ependyma-denuded ventricle surface percentage was quantified from micrographs (3-6 brain sections per animal) scanned under fluorescence in an Olympus VS120 microscope (Olympus, Tokyo, Japan) with a 10x objective.
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No products found
because this supplier's products are not listed.
Erin E. Henninger, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 3×30 seconds at 6 M/s and zirconium/silica beads (BioSpec Products 11079105z). Next ...
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No products found
because this supplier's products are not listed.
Yue Qu, et al.,
bioRxiv - Neuroscience 2021
Quote:
The completed constructs in M-6-attB-UAS-1-3-4 vector were amplified in Epi300 competent cells (EpiCentre) in LB-Chloramphenicol medium ...
-
No products found
because this supplier's products are not listed.
Anne Charlotte le Floch, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... were cultured O/N or 1h for SUP-T1 on dishes (Ibidi, Cat.No: 80136) precoated with poly-L-lysine ...
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No products found
because this supplier's products are not listed.
Rahima Ziane, Alain Camasses, Marta Radman-Livaja,
bioRxiv - Molecular Biology 2020
Quote:
... The membrane was incubated for 1h at room temperature with anti-H3K56ac (Active motif, 39281) at 1:2000 dilution ...
-
No products found
because this supplier's products are not listed.
Els F. Halff, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and incubated for 1h at RT with 1:1000 mouse anti-MAP2 (Synaptic Systems, 188-011), followed by 45-60 min incubation with the appropriate Alexa-fluorophore conjugated secondary antibodies (goat-anti-mouse Alexa405 ...