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Building Block
Sold for research purposes only.
Cat# 1028.0, SKU# 1028-100 mg,
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Isabel R.K. Kuebler, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... The MCH receptor antagonist 6-(4-Chloro-phenyl)-3-[3-methoxy-4-(2-pyrrolidin-1-yl-ethoxy)-phenyl]-3H-thieno[3,2-d]pyrimidin-4-one (GW803430) (Axon Medchem, Groningen, Netherlands) was freshly prepared the day of the treatment ...
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No products found
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Emily G. Kuiper, et al.,
bioRxiv - Biochemistry 2019
Quote:
... was monitored during unfolding over a linear temperature gradient (35-95 °C) over 3 minutes on a Tycho NT.6 instrument (NanoTemper). The temperature at which a transition occurs in a plot of the first derivative of the fluorescence ratio (350/330 nm) ...
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No products found
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Liam McCarthy, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Cell pellets of OD600 = 3-6 units (as measured using an Eppendorf BioPhotometer) were resuspended in 300 µL of 20 % TCA and 100 µL of acid-washed glass beads ...
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6 well glass bottom plate with high performance #1.5 cover glass (0.170±0.005mm). Black frame,...
Cat# P06-1.5H-N,
20/case, $249.00
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R. A. Petazzi, et al.,
bioRxiv - Biophysics 2020
Quote:
... 3-6 · 105 cells were plated onto 35 mm glass-bottom dishes (CellVis, Mountain View ...
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No products found
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A. Singer, L. Vinel, F. Trigo, I. Llano, M. Oheim,
bioRxiv - Neuroscience 2023
Quote:
Glutamate was photolysed from 4-Methoxy-7-nitroindoli-inyl-caged-L-glutamate (MNI-GLutamate, HelloBio, Bristol, UK) by focusing the beam of a 405-nm diode laser (Obis ...
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No products found
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Matthew Denholtz, et al.,
bioRxiv - Genomics 2019
Quote:
... cells were washed 3×3 minute in PBS and fixed for 30 minutes in 6% paraformaldehyde (Electron Microscopy Sciences 15710) in 1x PBS ...
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No products found
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Nittay Meroz, et al.,
bioRxiv - Evolutionary Biology 2023
Quote:
... The optical density was then measured using 6 automated plate readers (3 Epoch2 microplate reader - BioTek and 3 Synergy microplate reader - BioTek) simultaneously ...
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No products found
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Shigeo Takashima, et al.,
bioRxiv - Biochemistry 2020
Quote:
... 19-docosahexaenoic acid (22:6 ω-3, DHA, Tokyo Chemical Industry/TCI, Tokyo, Japan, #D2226). The FA standards were dissolved in a solution containing two volumes of chloroform and one volume of methanol with 0.05% (w/v ...
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No products found
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Zhibin Zhang, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Frizzled-3 (FZD3; Uniprot ID: Q9NPG1) and Frizzled-6 (FZD6; Uniprot ID: O60353) were synthesized by GenScript. For FZD1 ...
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No products found
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Samantha Sarni, et al.,
bioRxiv - Biochemistry 2020
Quote:
... The following day they were transfected with 6 μg Gag plasmid + 6 μg vector plasmid + 3 μg pCMV-Rev (to support nuclear export of vector RNA) using Transit-293 (Mirus) following the manufacturer’s protocol ...
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No products found
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Shelly J. Robertson, et al.,
bioRxiv - Microbiology 2023
Quote:
Sera were collected from SARS-CoV-2-infected mice at 3 and 6 dpi by centrifugation of whole blood in GelZ serum separation tubes (Sarstedt). BAL samples were recovered by insufflation of lungs with 1 ml sterile PBS followed by aspiration to collect ∼0.5ml volume of fluid ...
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No products found
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Raisa I. Krutilina, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... with 3 @g of either pCMV-6-Entry vector (OriGene, cat# PS100001, Rockville, MD) or pCMV-6-Entry expressing the CKB TrueORF (cat#RC203669) ...
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No products found
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Agata Szuba, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 5 mM dithiothreitol (DTT)) at 4°C overnight and concentrated to ~3-5 mg/mL using Vivaspin 6 concentrators (Sartorius, 6 ml, 100 kDa cut-off). Mammalian septin hexamers composed of mouse Sept2 (98.6% identical to human Sept2 ...
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Nathalia L. Malacco, et al.,
bioRxiv - Immunology 2023
Quote:
Female B6 mice at 6-8 weeks old were provided with 3% w/v DSS (MP Biomedicals) in drinking water ad libitum for 5 days ...
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Prepared to contain high collagenase activity with a caseinase to collagenase ratio of ~2:1....
Cat# LS005318,
100 mg, $60.00
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Miqdad O. Dhariwala, et al.,
bioRxiv - Immunology 2020
Quote:
... and skin was minced finely with dissection scissors and mixed in a 6-well plate with 3 ml of digestion buffer consisting of 0.8 mg/ml Collagenase Type 4 (4188; Worthington), 0.02 mg/ml DNAse (DN25-1G ...
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No products found
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Shijian Zhang, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Cymal-6 (Anatrace) was added to the Env(- ...
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No products found
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Yu-Cheng Chen, et al.,
bioRxiv - Bioengineering 2019
Quote:
... were coated with 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-5000] (DSPE-PEG, Nanocs) in order to reach a stable nanoparticle dispersion in aqueous solution ...
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No products found
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Charles J. Buchanan, et al.,
bioRxiv - Biophysics 2021
Quote:
6’-Sialyllactose sodium salt and 3’-Sialyllactose sodium salt were purchased from Carbosynth and used directly ...
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No products found
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Koetsu Inoue, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and anti-mouse PD-1 antibodies (clone RMP1-14, 10 mg/kg, 6 doses, every 3 days) were purchased from BioXcell. All drug treatments were administered intraperitoneally (i.p.).
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No products found
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Jacob Rose, et al.,
bioRxiv - Cell Biology 2021
Quote:
... peptide mixtures were transferred onto a trap chip (with 200 μm x 6 mm ChromXP C18-CL chip, 3 μm, 300 Å, SCIEX) and washed at 2 μL/min for 10 min with the loading solvent (H2O/0.1% formic acid) ...
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No products found
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R. A. Petazzi, et al.,
bioRxiv - Biophysics 2020
Quote:
... 3-6 · 105 cells were plated onto 35 mm glass-bottom dishes (CellVis, Mountain View, CA or MatTek Corp., Ashland, MA) 48 h prior to the experiment and transfected as described above ...
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No products found
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Yi-Hsuan Wu, et al.,
bioRxiv - Microbiology 2020
Quote:
The assay followed the protocol of 6-Phosphogluconic Acid (6-PGA) Assay Kit (Colorimetric) (BioVision). The cells were lysed and centrifuged to get clear extract ...
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No products found
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Wei Huang, et al.,
bioRxiv - Genetics 2023
Quote:
... 14-3-3 polyclonal antibody (Proteintech, Cat#14503-1-AP), and beta tubulin antibody (Abways Technology ...
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No products found
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Shane Breznak, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... 6 ug of rabbit IgG (Jackson Immunoresearch) or rat-HA antibodies for 3 hours with rotation at 4°C ...
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No products found
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Fanny Georgi, et al.,
bioRxiv - Microbiology 2020
Quote:
... 3’-deoxy-3’-fluorothymidine (DFT, CAS number 25526-93-6) was obtained from Toronto Research Chemical, North York ...
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No products found
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Seraphine Kamayirese, et al.,
bioRxiv - Biochemistry 2023
Quote:
(His)6-14-3-3ε was commercially obtained from Novus Biologicals (Centennial CO, USA), and 14-3-3ε-(His)12 was expressed in our laboratory (see details on protein expression in the supporting information) ...
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Anna V. Protasio, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Worms were rinsed in PBS (3×10mins, 3 × 1hr) and equilibriated in mounting media with 4’,6-diamidino-2-phenylindole DAPI (Fluoromount G, Southern Biotech, Birmingham, AL) overnight before mounting and imaging.
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No products found
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Dongdong Chen, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Mycoplasma testing was performed every 3-6 months using the Mycoplasma test kit (PromoCell, PK-CA91-1024) and on an ad hoc basis.
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No products found
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Prasath Paramasivam, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N [methoxy (polyethyleneglycol)-2000] (DMPE-PEG2000, NOF Corporation) and contained CleanCap® Enhanced Green Fluorescent Protein (eGFP) mRNA (5-methoxyuridine) (TriLink Biotechnologies, L-7201) and/or Clean Cap®Cyanine5 (Cy5 ...
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No products found
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Luis M. Rodríguez-Pérez, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The ependyma-denuded ventricle surface percentage was quantified from micrographs (3-6 brain sections per animal) scanned under fluorescence in an Olympus VS120 microscope (Olympus, Tokyo, Japan) with a 10x objective.
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No products found
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Fynn M. Hansen, et al.,
bioRxiv - Systems Biology 2020
Quote:
... samples were four-fold diluted with 1% TFA in isopropanol and loaded onto SDB-RPS cartridges (Strata™-X-C, 30 mg/ 3 ml or Strata™-X-C, 200 mg/ 6 ml, Phenomenex Inc.). Before peptide loading ...
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Patrick A. Carroll, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... anti-TXNIP (WB and IF, K0204-3, K0205-3, MBL International), anti-PGK1/2 (WB and IF ...
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6-Methoxy-2-naphthoic acid (6-Methoxy-2-naphthalenecarboxylic acid) is an modulator of NMDAR.
Cat# S5302, SKU# S5302-25mg,
25mg, $97.00
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Hui Zhang, et al.,
bioRxiv - Cell Biology 2020
Quote:
... with 6 μM CHIR 99021 (Selleck Chemicals) for 48 hours ...
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No products found
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Marine Tessier, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 6 colors cytometer Gallios (Beckman Coulter®). Gating strategy was determined based on the specific staining of markers for microglial population18.
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No products found
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Peng Liang, et al.,
bioRxiv - Microbiology 2020
Quote:
... and 6 μL DEPC-treated water (R1600,Solarbio) were mixed together as the PCRs (20 μL).
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No products found
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Natalia Benetti, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... 3 and 4 by lysing cells in the 6-well plate in RNA lysis buffer (Zymo) and freezing at −80 °C until extraction ...
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No products found
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Joel E. Graham, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 6 neoprene stoppers (RPI-259100-6) and capped with media bottle lid with a center bore to access the stopper ...
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No products found
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Ryann M. Fame, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 5–10 μL of CSF or serum was extracted in 4:6:3 chloroform:methanol:water mixture supplemented with isotopically labeled T3 and T4 (at 100 nM, Cambridge Isotope Laboratories ...
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No products found
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Tobias Jores, et al.,
bioRxiv - Plant Biology 2020
Quote:
... The center 8-10 cm of the second leaf from 7-9 plants were cut into thin strips perpendicular to the veins and immediately submerged in 10 mL protoplasting solution (0.6 M mannitol, 10 mM MES, 15 mg/mL cellulase R-10 [GoldBio], 3 mg/mL Macerozyme R-10 [GoldBio] ...
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No products found
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Joseph Atherton, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Single-axis cryo-electron tomography was performed at 3-6 μm defocus with a K2 Summit direct electron detector (Gatan) operating in counting mode (at 5 e-per pixel per second ...
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No products found
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I. Fayer, et al.,
bioRxiv - Biophysics 2020
Quote:
... 6 μM Biotin tubulin (T333P, Cytoskeleton), 3 μM Rhodamine tubulin (TL590M ...
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No products found
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Kevin J. McNaught, et al.,
bioRxiv - Molecular Biology 2020
Quote:
H3K27me2/3 ChIP using anti-H3K27me2/3 antibody (Active Motif, 39536) was performed as previously described (12 ...
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No products found
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Candida Wong, et al.,
bioRxiv - Immunology 2020
Quote:
Flow cytometry data was analysed using the FCS Express 6 Flow Cytometry Software version 6 (De Novo Software). CHO cells were primarily gated by forward scatter height (FSC-H ...
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No products found
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Nikita Raj, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 5-6 × 105 siRNA transfected or untransfected cells were cultured on collagen-coated 6-well plates (Ibidi, 80287) until 90-100 % confluency (20-24 hours) ...
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No products found
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I Ladero-Auñon, et al.,
bioRxiv - Immunology 2021
Quote:
... and IL-8 (3114-1A-6 MABTECH) release in 24h culture supernatants using the provided manufacturer’s instructions ...
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No products found
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Sophie Lanciano, et al.,
bioRxiv - Genomics 2023
Quote:
Two micrograms of genomic DNA were sonicated for 6 cycles (6 s on, 90 s off) at 4 °C with a Bioruptor NGS (Diagenode), generating average fragments of 1 kb ...
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No products found
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Chhiring Lama, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... 6-diamidino-2-phenylindole (Spectral DAPI, Akoya Biosciences) was applied per provided protocols to label nuclei ...
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No products found
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Ashwathi Rajeevan, Riya Keshri, Sachin Kotak,
bioRxiv - Cell Biology 2020
Quote:
Double-stranded siRNA oligonucleotides used were 5’- CAGUACCAGUGAGUGGCCCCACCUG-3’ (NuMA 3’UTR siRNA; Eurogentec) and 5’-CACCGUGUGUCUAAGCAAA-3’ (RCC1 siRNA ...
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No products found
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Brendan P. Lehnert, et al.,
bioRxiv - Neuroscience 2021
Quote:
... 3 mm WD objective (Nikon) with scan mirror excursions that produced a 510 x 510 μm2 field of view ...
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No products found
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Yuuki Wittmer, et al.,
bioRxiv - Biophysics 2022
Quote:
... ∼6 mL using Millipore Amicon Ultra-15 3 kDa MWCO centrifugal filter and dialyzed (Spectrum Laboratories 1.7 ml/cm standard SpectraPor 1 RC Tubing ...