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No products found
because this supplier's products are not listed.
Kayla Glockzin, Thomas D Meek, Ardala Katzfuss,
bioRxiv - Biochemistry 2021
Quote:
... 9-deazaadenine was purchased from AA blocks (AA0037QI). Unless otherwise indicated ...
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No products found
because this supplier's products are not listed.
Kyla D Omilusik, et al.,
bioRxiv - Immunology 2021
Quote:
... 1-1.5 mg of protein lysate was incubated with Id2 (9-2-8, CalBioeagents) or Id3 (6-1, CalBioreagents) antibody overnight at 4°C followed by protein A/G agarose beads (Santa Cruz ...
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No products found
because this supplier's products are not listed.
Shivnarayan Dhuppar, Aprotim Mazumder,
bioRxiv - Cell Biology 2019
Quote:
... DNA hybridization mixture was prepared: 9 µl hybridization buffer (from Empire Genomics) + 1 µl probes ...
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No products found
because this supplier's products are not listed.
Simon Schäper, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5-bromo-4-chloro-3-indolyl β-d-galactopyranoside (X-Gal, Apollo Scientific) was used at 100 μg/ml ...
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No products found
because this supplier's products are not listed.
Steven A Kemp, et al.,
bioRxiv - Microbiology 2021
Quote:
... 9 DNA fragments with overlap sequences were amplified by PCR from a plasmid (phCMV1, Genlantis) encoding the full-length SARS-CoV-2 S gene (BetaCoV/Wuhan-Hu-1/2019 ...
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No products found
because this supplier's products are not listed.
Sebiha Cevik, et al.,
bioRxiv - Genetics 2023
Quote:
... Worms were initially transferred onto a 2.5% agarose pad prior (Halocarbon oil, Sigma: 9002-83- 9), followed by microinjection ...
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No products found
because this supplier's products are not listed.
Shail Kabrawala, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Affinity-purified chicken anti-WHIMP antibodies were generated against mouse WHIMP amino acids 9-21 (Aves Labs). Other antibodies were rabbit anti-MBP [68] ...
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No products found
because this supplier's products are not listed.
Peter M. F. Emmrich, et al.,
bioRxiv - Genomics 2020
Quote:
Predicted protein sequences from 9 species (Cicer arietinum, Cucumis sativus, Fragaria vesca, Glycine max, Malus domestica, Medicago truncatula ...
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No products found
because this supplier's products are not listed.
Yuyeon Jang, et al.,
bioRxiv - Immunology 2022
Quote:
... immunofluorescence analysis and Western blotting were performed as described previously (9) using a SARS-CoV2 polyclonal antibody (Elabscience, USA).
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No products found
because this supplier's products are not listed.
Rishi Drolia, et al.,
bioRxiv - Microbiology 2023
Quote:
Listeria in the extra-intestinal sites was enumerated in the organs that were harvested aseptically following oral infection and homogenized using a tissue homogenizer in 4.5 ml (spleen and MLN) or 9 ml (liver) of buffered-Listeria enrichment broth (BLEB; Neogen) containing 0.1% Tween 20 and selective antimicrobial agents (Neogen) ...
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No products found
because this supplier's products are not listed.
Mansi Prakash, et al.,
bioRxiv - Neuroscience 2021
Quote:
... and tissue was triturated for about 1 min (90% tissue dispersal) using a 9” sterile silanized glass Pasteur pipette (BrainBits), avoiding air bubbles ...
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No products found
because this supplier's products are not listed.
Amala John, et al.,
bioRxiv - Plant Biology 2023
Quote:
45-50 inflorescence meristems for each of three biological replicates of Col-0 and clv3-9 were collected and total RNA isolated using the EZNA Plant RNA kit (Omega Bio-tek). RNA was treated with RNase-free DNase (Omega Bio-tek ...
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No products found
because this supplier's products are not listed.
Brianna Romer, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... GOPTS was then reacted with a mixture of 9 parts α-hydroxy-ω-amino PEG 3000 to 1 part α-biotinyl-ω-amino PEG 3000 by weight (Rapp Polymere). Flow cells for imaging were made using double-sided tape applied to a glass slide and passivated with 2 mg/mL PLL20-g[3,5]-PEG2 (SuSOS AG) ...
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No products found
because this supplier's products are not listed.
Minerva Rodriguez, et al.,
bioRxiv - Neuroscience 2023
Quote:
... the territorialized home-cage (10½ X 19 X 6⅛; N40 cage; Ancare, Bellmore, NY) of a pair-bonded couple (male and female ...
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No products found
because this supplier's products are not listed.
Emma Laporte, et al.,
bioRxiv - Developmental Biology 2022
Quote:
WT (C57BL/6) neonatal mice (PD5) were treated twice with 5 μg LGK-974 (Biogems, Westlake Village, CA) per g bodyweight or vehicle (corn oil ...
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No products found
because this supplier's products are not listed.
Xiaona Chen, et al.,
bioRxiv - Cell Biology 2020
Quote:
... gastrocnemius and quadriceps muscles were injected with CTX (Latoxan; 10−5 M). At the indicated time points (3- and 7-day post injury) ...
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No products found
because this supplier's products are not listed.
Mamoru Ishii, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... we mixed Hoechst 33342 (5 μg/ml, Dojindo Molecular Technologies, #H342-10) or DAPI (Dojindo Molecular Technologies ...
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No products found
because this supplier's products are not listed.
Siranush Babakhanova, et al.,
bioRxiv - Molecular Biology 2021
Quote:
Two-photon spectrum and cross sections of TagRFP658 were measured in PBS buffer at concentrations ∼1–5·10−5 M in 1 mm glass spectroscopy cuvettes (Starna cells) using an MOM two-photon fluorescent microscope (Sutter Instrument ...
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No products found
because this supplier's products are not listed.
Rohan Dandage, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
... 0.5 μl of each primer at 10 μM and 0.15 μl of Taq DNA Polymerase (5 U/μl, BioShop Canada Inc.), in a final volume of 25 μl ...
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No products found
because this supplier's products are not listed.
Kanishk Jain, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 5 μL of GST-tagged reader domain was incubated with 5 μL of 10 nM biotinylated nucleosomes (e.g., EpiCypher #16-9001) for 30 minutes at room temperature in 20 mM HEPES pH 7.5 ...
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No products found
because this supplier's products are not listed.
Koral Goltseker, et al.,
bioRxiv - Neuroscience 2022
Quote:
... the mice were habituated to collect 5-6 sucrose pellets (45 mg, Dustless Precision Pellets, Bio-Serv, Frenchtown, NJ, USA) from a plastic plate inserted into the home cage ...
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No products found
because this supplier's products are not listed.
Anne Berthold, Vett Lloyd,
bioRxiv - Molecular Biology 2022
Quote:
... starting material for the exposure experiments were HUVECs in passage 3 and HEK-293 cells in passage 5 seeded at a density of 4000 cells/cm2 into 6-well tissue culture plates (Celltreat, 229105) in antibiotic-containing media ...
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No products found
because this supplier's products are not listed.
Lotta Pohjolainen, Heikki Ruskoaho, Virpi Talman,
bioRxiv - Cell Biology 2022
Quote:
... were dissociated and seeded at a density of 700,000-800,000 cells/well in RB+ supplemented with 10% fetal bovine serum (FBS) on flexible collagen I-coated 6-well BioFlex® culture plates (Flexcell International Corporation ...
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No products found
because this supplier's products are not listed.
Susanne Reinhold, et al.,
bioRxiv - Cell Biology 2023
Quote:
... the cell pellet was washed with 5-10 ml FACS buffer (1% FBS (Thomas Scientific; USA), 1mM EDTA (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Xinwen Zhu, Chiara Ricci-Tam, Allyson E. Sgro,
bioRxiv - Molecular Biology 2022
Quote:
... diluted 1:5000 in 5% milk in PBST (10 mM sodium phosphate, 0.15M NaCl, 0.05% Tween-20, pH 7.5, BioWORLD) at 4°C ...
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No products found
because this supplier's products are not listed.
S. Jake Gonzales, et al.,
bioRxiv - Immunology 2021
Quote:
... 0.7 µl template switch oligo (TSO) (10 µM, f/c 200 nM, IDT DNA; #110, Supplementary table 5), nuclease-free water till 35 µl and subsequent incubation at 42°C for 2 hours ...
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No products found
because this supplier's products are not listed.
Daniel P Myatt, et al.,
bioRxiv - Biophysics 2022
Quote:
... (6) using plasmid DNA purchased from Aldevron, UK with the reaction optimised as per the design-of-experiment (DOE ...
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No products found
because this supplier's products are not listed.
Charles Kyriakos Vorkas, et al.,
bioRxiv - Immunology 2020
Quote:
Tissues were resected and underwent three minutes of bead beating at speed 6 using 10 2.0 mm zirconium oxide beads/sample in 1 mL (lungs and spleen) or 5 beads/sample in 0.5 mL of RPMI/10% fetal bovine serum in a Bullet Blender (Next Advance). Homogenized tissues were passed through 100 um strainers ...
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No products found
because this supplier's products are not listed.
Hartwig Seitter, et al.,
bioRxiv - Neuroscience 2020
Quote:
... In most experiments further gentle dissociation was achieved by shaking the tube for 5-10 min on the slowest setting of a vortexer (Vortex-Genie 2, Scientific Industries). Dissociated cells were seeded on coated coverslips (Ø 12 mm ...
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No products found
because this supplier's products are not listed.
Kevin R. Coffey, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Tissue was stained in a 5 % potassium ferrocyanide and 10 % HCl and counterstained for antibodies against calbindin D28k immunohistochemistry (ImmunoStar, Inc., Hudson WI), as described previously(Coffey et al. ...
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No products found
because this supplier's products are not listed.
Tianhu Sun, et al.,
bioRxiv - Plant Biology 2022
Quote:
... and LHCB1-6 (Cat#AS01011) antibodies were purchased from Agrisera. A secondary goat-anti-rabbit HRP-conjugated antibody (BioRad cat#1706515 ...
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No products found
because this supplier's products are not listed.
Armin Bayati, et al.,
bioRxiv - Cell Biology 2022
Quote:
... was then conjugated with 5 nm gold beads (Cytodiagnostics, cat# CGN5K-5-2), immediately before experimental use ...
-
No products found
because this supplier's products are not listed.
Ju-Chan Park, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... and maintained for 6 days in myoblast cell culture medium (SKM02, Amsbio). At day 12 ...
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No products found
because this supplier's products are not listed.
Sven Klumpe, et al.,
bioRxiv - Biophysics 2021
Quote:
... 5 ug/ml Insulin (Cell Applications), 10 mM HEPES (pH 7.4) ...
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No products found
because this supplier's products are not listed.
Yanan Yang, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 5 ng/mL insulin (CELL technologies), 25 ng/mL hydrocortisone (CELL technologies) ...
-
No products found
because this supplier's products are not listed.
Orsolya Németh-Szatmári, et al.,
bioRxiv - Molecular Biology 2022
Quote:
6 × 105 cells/flask were seeded into T25 cell culture flasks (Biologix, Jinan, Shandong, China) and left to grow for 24 h ...
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No products found
because this supplier's products are not listed.
Tanja Bhuiyan, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... cells were incubated in 2 μg/mL 4′,6-diamidino-2-phenylindole (DAPI, GeneCopoeia, C002) in PBS for 5 min at RT ...
-
No products found
because this supplier's products are not listed.
Kimberly L. Cramer-Morales, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Total genomic 5-methylcytosine and 5-hydroxymethylcytosine levels were measured in genomic DNA with the corresponding immunoassay systems also from EpiGentek according to the manufacturer’s instructions.
-
WB,ELISA
Cat# A5074, SKU# A5074-100ul,
100ul, $157.00
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Alexey A. Gavrilov, et al.,
bioRxiv - Genomics 2019
Quote:
... Cells were washed with cold PBS and incubated in 375 µL lysis buffer (10 mM Tris pH 7.5, 10 mM NaCl, 0.2% NP40, 1× protease inhibitors (Bimake), 37.5 U SUPERase.In RNase inhibitor (Invitrogene) ...
-
No products found
because this supplier's products are not listed.
Yuta Kudo, et al.,
bioRxiv - Biochemistry 2020
Quote:
... TSKgel Amide-80 HR (5 μm, 4.6 × 250 mm, Tosoh), with CH3CN:H2O:formic acid (30:70:0.002 ...
-
No products found
because this supplier's products are not listed.
Wakana Sato, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... The readings were performed by measuring the luminescence of all the wavelengths with readings “6” and photomultiplier tube (PMT) setting “medium” ...
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No products found
because this supplier's products are not listed.
Alena Rudkouskaya, et al.,
bioRxiv - Bioengineering 2019
Quote:
... The excitation was set to 750 nm, and the emission filter were 820±6 nm (Semrock, FF01-820/12-25) and 810±45 (Chroma Technology, ET810/90). The imaging parameters were set the same for all mice.
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No products found
because this supplier's products are not listed.
Natalie C. Butterfield, et al.,
bioRxiv - Genetics 2019
Quote:
... harboring alanine/alanine or threonine/threonine polymorphisms for the deiodinase 2 gene at codon 92 (Dio2Ala92, n=9 and Dio2Thr92, n=10) were generated by Applied Stemcell Inc ...
-
No products found
because this supplier's products are not listed.
Christopher A Jackson, et al.,
bioRxiv - Genomics 2019
Quote:
... We confirmed that glucose in the media was exhausted between hour 9 and hour 10 using a hexokinase-based assay (R-Biopharm #10716251035). All other steps of harvesting cells were identical to the 4 hour experiments.
-
No products found
because this supplier's products are not listed.
Wei Wei, et al.,
bioRxiv - Biochemistry 2024
Quote:
... blood plasma was collected at 9 am and ELISA kits were used following manufacturer’s instructions (Leptin: Crystal Chem, 90030 ...
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No products found
because this supplier's products are not listed.
Eric Blanc, et al.,
bioRxiv - Genomics 2019
Quote:
For immunisation 8-12-week old ABabDII mice were injected subcutaneously with 100 µg of mutant short peptide (9-10mers, JPT) supplemented with 50 µg CpG 1826 (TIB Molbiol), emulsified in incomplete Freund’s adjuvant (Sigma) ...
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No products found
because this supplier's products are not listed.
Tsuyoshi Waku, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... After the treatment with 5 nM or 10 nM bortezomib (BTZ, Peptide Institute), the cells were stained by Trypan Blue and automatically counted using a TC20 Automated Cell Counter (Bio-Rad Laboratories).
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No products found
because this supplier's products are not listed.
Cathal Meehan, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Recombinant IL-6 with an N-terminal his tag (Fitzgerald, Biosynth Ltd.) was immobilized on His-Pur™ Ni-NTA Resin (ThermoScientific ...
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No products found
because this supplier's products are not listed.
Yildiz Koca, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... blocked in 5% skim milk (Labscientific) and incubated with primary rabbit anti-Abl or mouse anti-NotchICD ...
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No products found
Mohummad Aminur Rahman, et al.,
bioRxiv - Cancer Biology 2020
Quote:
Human ATG7 shRNA-GFP (5’-CAGTGGATCTAAATCTCAAACTGAT-3’) and scrambled control shRNA-GFP (5’-GGGTGAACTCACGTCAGAA −3’) lentiviral plasmids were purchased from Applied Biological Materials Inc ...