-
No products found
because this supplier's products are not listed.
Yahor Savich, et al.,
bioRxiv - Biophysics 2019
Quote:
... and RLC was incubated at 4°C for 1hr in 5-fold molar excess of bifunctional 3,4-Bis-(methanethiosulfonylmethyl)-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-1-yloxy spin label (BSL; Toronto Research Chemicals). The covalent disulfide double bond between BSL and the 𝛼-helix backbone produces bifunctional stereospecific attachment in this labeling conditions (Fig ...
-
No products found
because this supplier's products are not listed.
François Halloy, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 5-(Benzylthio)-1H-tetrazole (Carbosynth) was used at 0.24 M concentration in dry acetonitrile to activate the phosphoramidites for coupling ...
-
No products found
because this supplier's products are not listed.
Yongchan Lee, et al.,
bioRxiv - Biochemistry 2021
Quote:
... and L-[3H]alanine (5 Ci/mol; Moravek Biochemicals)) were measured for 3 min in Na+-free HBSS pH 7.4 ...
-
No products found
because this supplier's products are not listed.
A. Kumar, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and blocked for 1h at room temperature (RT) or overnight at 4 °C in 5% normal donkey serum (NDS) in PBS-TX (Jackson Immunoresearch). Primary antibodies were chicken anti-GFP (1:1000 ...
-
No products found
because this supplier's products are not listed.
Súil Collins, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... The devices were silanized by pipetting a fresh solution of 2% Trichloro(1H,1H,2H,2H-perfluorooctyl)silane in HFE-7500 (3M) into the chips ...
-
No products found
because this supplier's products are not listed.
Xavier Grau-Bové, et al.,
bioRxiv - Evolutionary Biology 2022
Quote:
... followed by another round of centrifugation at 21,000xg 4°C for 1h (Eppendorf, Centrifuge 5424R). Pellets were stored at −70°C ...
-
No products found
because this supplier's products are not listed.
Manu De Groeve, Bram Laukens, Peter Schotte,
bioRxiv - Microbiology 2023
Quote:
... 2 L or 5 L scale (Biostat B-plus and B-DCU units, Sartorius Stedium Biotech) as previously described [PMID ...
-
No products found
because this supplier's products are not listed.
Yang Liu, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... and incubated with 4′,6-diamidino-2-phenylindole (Solarbio, #C0065) for 10 min ...
-
No products found
because this supplier's products are not listed.
Walden Li, Ryan M. Wyllie, Paul A. Jensen,
bioRxiv - Microbiology 2020
Quote:
... Strains carrying the pLacZ plasmid appear blue when plated on 0.008% X-gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Zymo Research, CA, USA). X-gal concentrations above 0.008% appear to inhibit growth of S ...
-
No products found
because this supplier's products are not listed.
Dillon S. McDevitt, et al.,
bioRxiv - Neuroscience 2019
Quote:
... recording electrodes (2-5 MΩ; borosilicate glass capillaries (WPI #1B150F-4) pulled on a horizontal puller from Sutter Instruments (model P-97) ...
-
No products found
because this supplier's products are not listed.
Dougald M. Monroe, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 4 μM lipid vesicles (Avanti Polar Lipids PS:PC:PE 3:2:5), and FV (0 ...
-
No products found
because this supplier's products are not listed.
Paulina M. Wojnarowicz, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... 5 and 6 hours after addition of the reagent using a plate reader (Synergy 2, BioTek).
-
No products found
because this supplier's products are not listed.
Abishek Chandrashekar, et al.,
bioRxiv - Microbiology 2022
Quote:
... SARS-CoV-2 (B.1.1.529) RBD proteins (Sino Biological) labeled with APC and DyLight 405 ...
-
No products found
because this supplier's products are not listed.
Jie Hu, et al.,
bioRxiv - Microbiology 2021
Quote:
... SARS-CoV-2 B.1.617 and B.1.1.7 variant Spikes were codon-optimized and synthesized by GenScript Inc (Nanjing ...
-
No products found
because this supplier's products are not listed.
Lunhua Liu, Kazuyo Takeda, Mustafa Akkoyunlu,
bioRxiv - Immunology 2020
Quote:
Splenic B cells were isolated from 5 or 12 weeks old MRL/Lpr mice using B Cell Isolation Kit (Miltenyi Biotec) and stained with CSFE before co-culturing with purified TCRβ+CD138+ or TCRβ+CD138− cells in the presence of anti-CD3/CD28 antibodies (BD Biosciences) ...
-
No products found
because this supplier's products are not listed.
Trinovita Andraini, et al.,
bioRxiv - Neuroscience 2021
Quote:
... 3-(2,4-Dichloro-5-methoxyphenyl)-2-sulfanyl-4(3H)-quinazolinone (BML-CM127-0050, Enzo Life Sciences) was injected at 20mg/kg (in 10% DMSO ...
-
Cat# HY-W036553,
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Aleksandra Tempes, et al.,
bioRxiv - Cell Biology 2023
Quote:
... cells were treated for 2 h with 60 µM 1-adamantyl(5- bromo-2-methoxybenzyl)amine (ABMA; Medchemexpress, catalog no. HY-124801) or 50 µM chloroquine (dissolved in water ...
-
No products found
because this supplier's products are not listed.
Maxime Penisson, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 6 % agarose (LE-8200-B, Euromedex) PBS solution ...
-
No products found
because this supplier's products are not listed.
Christoph G. Gäbelein, et al.,
bioRxiv - Cell Biology 2021
Quote:
... (B) Coupling mitochondrial extraction with transplantation from individual cell to cell: One quadrant of a 4-well micro-insert (ibidi) was seeded with recipient HeLa (su9-BFP ...
-
No products found
because this supplier's products are not listed.
Francesco Roncato, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Images were acquired at a rate of one frame every 4-5 min for 4 h using an IX83 inverted microscope (Olympus) equipped with UPlanFLN 20x/0.50 Ph1 ∞/0.17/FN 26.5 objective (Olympus) ...
-
No products found
because this supplier's products are not listed.
Priyadharshini Prabhakar, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 0.4 μM 5/6- TAMRA-PEG 4 -Alkyne (Jena Bioscience; Jena, Germany; CLK-TA108) and 0.2 mM freshly prepared CuSO4 in 1x PBS pH7.8 ...
-
Chromatographically purified. A solution in 100 mM sodium chloride. Chymotrypsin and trypsin ² 0.02%.
Cat# LS005302,
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Wei Wei, et al.,
bioRxiv - Biochemistry 2024
Quote:
... epidydimal fat from 4-month-old male DIO C57BL/6J mice was dissected out and dissociated using 2 mg/ml Collagenase B (Worthington, CLSAFB) and 1 mg/ml soybean trypsin inhibitor (Worthington ...
-
No products found
because this supplier's products are not listed.
Bharat Ravi Iyengar, Andreas Wagner,
bioRxiv - Evolutionary Biology 2021
Quote:
... and 6-(2-deoxy-beta-D-ribofuranosyl)-3,4-dihydro-8H-pyrimido-[4,5-C] [1,2]oxazin-7-one triphosphate (dPTP, Trilink Biotechnologies), 200nM each of forward and reverse primers ...
-
No products found
because this supplier's products are not listed.
Emma E. Kovak, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... one 2 microgram portion received 5 units of RNase R (Lucigen, Middleton, WI) and the other an equal volume of nuclease-free water (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Julia Hartmann, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 2 *106 cells were transferred into one well of a new 6-well plate (#83.3920, Sarstedt, Germany) in 4 mL medium ...
-
No products found
because this supplier's products are not listed.
Sergii Palchevskyi, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... 5-6 mM LMNG (Anatrace) and 1-1.2 mM Cholesteryl Hemisuccinate Tris Salt (CHS ...
-
No products found
because this supplier's products are not listed.
Michael Brandon Ware, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... anti-IL-6 or anti-CTLA-4 antibodies (BioXCell). For CXCR3 blockade studies ...
-
No products found
because this supplier's products are not listed.
Sebastiaan Pieter van Kessel, et al.,
bioRxiv - Microbiology 2021
Quote:
... Samples were analysed on a C18 column (Kinetex 5 μM, C18 100 Å, 250 × 4·6 mm, Phenomenex, Utrecht, The Netherlands) using a gradient of water/methanol with 0·1% formic acid (0–3 min ...
-
No products found
because this supplier's products are not listed.
Catherine Horiot, et al.,
bioRxiv - Immunology 2023
Quote:
... recombinant human IL-6 (2 ng/mL) (Proteintech) was added to RPMI1640 culture medium containing Ultraglutamine (Lonza ...
-
No products found
because this supplier's products are not listed.
Pan Gong, et al.,
bioRxiv - Plant Biology 2021
Quote:
Confocal microscopy was performed using a Leica TCS SP8 point scanning confocal microscope (Figure 2, 3h upper panel, Supplementary figures 8, 9, 10a, and 10d) or Zeiss LSM980 confocal microscope (Carl Zeiss) (Figure 3e ...
-
No products found
because this supplier's products are not listed.
Janne J.M. van Schie, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... 2 mg of chromatin lysate was rotated overnight at 4°C with 5 μg antibody: rabbit IgG (Diagenode #C15410206), anti-NIPBL (NIPBL#1 ...
-
No products found
because this supplier's products are not listed.
Mario Mietzsch, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... 3.5 μL was applied to a glow-discharged Quantifoil copper grid with 2 nm continuous carbon support over holes (Quantifoil R 2/4 400 mesh), blotted ...
-
No products found
because this supplier's products are not listed.
Shulan Xiao, Saumitra Yadav, Krishna Jayant,
bioRxiv - Neuroscience 2022
Quote:
... 4 to 6 MΩ borosilicate patch pipette (Sutter Instruments, CA, USA) were pulled using a P1000 pipette puller (Sutter Instruments ...
-
No products found
because this supplier's products are not listed.
Ancély F. dos Santos, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Fluorescence intensity values were collected every 2 min during 1h at 500 nm in a SpectraMax M2 microplate reader (Molecular Devices). Activity units were calculated as ...
-
No products found
because this supplier's products are not listed.
Nina Jurčić, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Patch pipettes (4-6 MΩ) were pulled from borosilicate glass capillaries (Harvard Apparatus) using a horizontal P-97 Flaming/Brown type micropipette puller (Sutter Instrument Co) ...
-
No products found
because this supplier's products are not listed.
Morgan E. Mouchka, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
... Colonies were screened on LB plates with 50 μg/mL ampicillin and 9.5 mg/mL X-gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside; APExBIO) and Sanger sequencing of the J was used to confirm cloning and successful transformation of the ancestral λ sequence.
-
No products found
because this supplier's products are not listed.
Yusuke Ohno, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 1H NMR (CDCl3; Cambridge Isotope Laboratories ...
-
No products found
because this supplier's products are not listed.
Eva Dervas, et al.,
bioRxiv - Pathology 2020
Quote:
... followed by a 15 min incubation with DAPI (4′, 6-diamidino-2-phenylindole, Novus Biologicals; 1:10,000 in PBS). Sections were washed twice with distilled water ...
-
No products found
because this supplier's products are not listed.
L. Camborde, et al.,
bioRxiv - Plant Biology 2020
Quote:
... 50μg of total proteins were incubated 3H at 4°C with 30μl of GFP-Trap Agarose beads (Chromotek; gta-20) under gentle agitation for GFP-tagged protein purification ...
-
No products found
because this supplier's products are not listed.
Amy R. Rappaport, et al.,
bioRxiv - Immunology 2021
Quote:
... Membranes were blocked for 1h in 5% skim milk in TBST and then probed with an anti-S2 mouse monoclonal antibody (GeneTex) at a 1:1000 dilution for 2h-overnight ...
-
No products found
because this supplier's products are not listed.
Hillary A. Wadsworth, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and 5-(3-Bromophenyl)-1,3-dihydro-2H-benzofuro[3,2-e]-1,4-diazepin-2-one (5-BDBD) (cat. no. T22518, TargetMol, Wellesley Hills, Massachusetts, USA) were dissolved in stock solutions and then diluted into ACSF at specified concentrations (50 µM IVM ...
-
No products found
because this supplier's products are not listed.
Amin Zargar, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... 5-methyl-6-(propan-2-yl)oxan-2-one was synthesized by Enamine (Cincinnati, USA) to greater than 95% purity.
-
No products found
because this supplier's products are not listed.
Jossana A. Damasco, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Sulfo-Cy5.5-NHS ester (potassium 3-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-1,1-dimethyl-2-((1E,3E,5E)-5-(1,1,3-trimethyl-6,8-disulfonato-1,3-dihydro-2H-benzo[e]indol-2-ylidene)penta-1,3-dien-1-yl)-1H-benzo[e]indol-3-ium-6,8-disulfonate) was obtained from Lumiprobe (Hunt Valley, MD, USA).
-
No products found
because this supplier's products are not listed.
Pazhanichamy Kalailingam, et al.,
bioRxiv - Pathology 2024
Quote:
... then incubated overnight at 37°C with staining buffer (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside [X-gal, Bioline, London ...
-
No products found
because this supplier's products are not listed.
Adrien Birot, et al.,
bioRxiv - Genomics 2021
Quote:
... Samples were incubated 1h at 4°C with anti-MYC magnetic beads (TA150044, Origene). Beads were washed 5 times with lysis buffer without inhibitors ...
-
No products found
because this supplier's products are not listed.
Surya Prakash Rao Batta, et al.,
bioRxiv - Cell Biology 2022
Quote:
HUVECs (passage 2 to 6; PromoCell) were routinely cultured in EBM media supplemented with the provided growth factors kit (Promocell) ...
-
No products found
because this supplier's products are not listed.
Rohan Kodgule, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133 ...
-
No products found
because this supplier's products are not listed.
Aaron T. Crain, et al.,
bioRxiv - Genomics 2024
Quote:
... Membranes were blocked in 5% milk in TBS- Tween for 1h prior to incubation with α-GFP (1:1000, Rockland) or α-Set8 (1:1000 ...
-
No products found
because this supplier's products are not listed.
Eugene V. Ravkov, et al.,
bioRxiv - Immunology 2023
Quote:
... SARS-CoV-2 infection was assessed by one or more immunologic (Abbott Architect SARS-CoV-2 IgG ...
-
No products found
because this supplier's products are not listed.
Alice L. Herneisen, et al.,
bioRxiv - Microbiology 2023
Quote:
... The membrane was incubated in secondary antibody solution (1:10,000 Goat anti-Mouse IgG IRDye 800 or 680) in 5% milk/TBS-T for 1h at room temperature and was visualized by LI-COR Odyssey CLx ...