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No products found
because this supplier's products are not listed.
Morgan E. Mouchka, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
... Colonies were screened on LB plates with 50 μg/mL ampicillin and 9.5 mg/mL X-gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside; APExBIO) and Sanger sequencing of the J was used to confirm cloning and successful transformation of the ancestral λ sequence.
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No products found
because this supplier's products are not listed.
Subash Godar, et al.,
bioRxiv - Biophysics 2021
Quote:
... We detected the bound alkaline phosphatase labeled antibodies by incubating the membrane in BCIP/NBT (5-bromo, 4-chloro, 3- indolylphosphate/nitro-blue tetrazolium, AMRESCO, LLC.) substrate for 30–45 minutes ...
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No products found
because this supplier's products are not listed.
Caterina Di Sano, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 1% nonessential amino acids and 2□mM L‐glutamine (all from Euroclone) was used for culturing A549 and Colo 699.The cells were maintained in an incubator at 37□°C with a humidified atmosphere with 5% CO2and were maintained as adherent monolayers ...
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No products found
because this supplier's products are not listed.
Kazuhiro Hayashi, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 25mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)(Research Products International) or acidic pH 6.5 media (26mM NaHCO3 ...
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No products found
because this supplier's products are not listed.
Michael Bouyer, et al.,
bioRxiv - Bioengineering 2020
Quote:
... two conditions were further studied in larger groups with an intermediate dose of BMP-2 (loading at 50 μg/ cm3 and 110 μg/ cm3) (n=6 for low BMP-2 dose, n=5 for high dose) to perform statistical analysis ...
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4-Amino-5-imidazolecarboxamide is an imidazole derivative which is a metabolite of the...
Cat# S3651, SKU# S3651-25mg,
25mg, $97.00
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Liying Chen, et al.,
bioRxiv - Neuroscience 2023
Quote:
The D1 receptor antagonist R(+)-7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepi ne hydrochloride (SCH-23390, Selleck chemicals, China) was dissolved in 0.9% saline and stored -80°C ...
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No products found
because this supplier's products are not listed.
Kailin Yin, et al.,
bioRxiv - Immunology 2023
Quote:
... 6 million cells were resuspended in 4 ml of PBS (Rockland) containing 2 mM EDTA (Corning ...
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No products found
because this supplier's products are not listed.
Yue Qu, et al.,
bioRxiv - Neuroscience 2021
Quote:
The completed constructs in M-6-attB-UAS-1-3-4 vector were amplified in Epi300 competent cells (EpiCentre) in LB-Chloramphenicol medium ...
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No products found
because this supplier's products are not listed.
He-Chin Hsieh, et al.,
bioRxiv - Microbiology 2023
Quote:
... and BA.4/5 (Genetex, Cat. No. GTX137098-pro) were mixed with RBD-LTA ...
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No products found
because this supplier's products are not listed.
Agata Stepien, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... rat antibodies for the detection of phosphorylated RNAPII (serine 5 and serine 2) (Chromotek, 1: 100) and secondary goat anti-rat Alexa Fluor 488 antibodies (A-11006 ...
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No products found
because this supplier's products are not listed.
James P Bridges, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 5 % CO2 with 20 µl Calcium 6 loading buffer (Molecular Devices, Cat #R8191): 1x HBSS (Gibco ...
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No products found
because this supplier's products are not listed.
Alexa R. Anderson, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Sections were then developed using the respective Polink-2 Plus HRP with DAB kit (Origene, D87-6 Hamster D39-6 Rabbit). Sections were counterstained in Hematoxylin (EMS ...
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No products found
because this supplier's products are not listed.
Bangmin Liu, et al.,
bioRxiv - Cell Biology 2022
Quote:
... the pTr1 cells (2×105 cells/2 ml/well) were seeded in 6-well plates (83.3920; Sarstedt, Nümbrecht, Germany) in CM until the monolayer reached 80-90% confluence ...
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WB, IHC, IF,ELISA
Cat# A5442, SKU# A5442-100ul,
100ul, $157.00
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Kristina A.M. Arendt, et al.,
bioRxiv - Cancer Biology 2020
Quote:
In vitro cell proliferation was determined using WST-8 [water soluble tetrazolium-8 or 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulphophenyl)-2H-teterazolium] assay (Bimake; Munich, Germany). For this ...
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No products found
because this supplier's products are not listed.
Rachel E. Young, et al.,
bioRxiv - Bioengineering 2022
Quote:
... The apparent pKa of LNPs was determined via TNS [6-(p-toluidinyl)naphthalene-2-sulfonic acid] (Thomas Scientific, Swedesboro, NJ) assays ...
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No products found
because this supplier's products are not listed.
Miguel Alejandro Lopez-Ramirez, et al.,
bioRxiv - Biochemistry 2019
Quote:
... and 2-amino-N-(1-phenylethyl)benzamide (Enamine).
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No products found
because this supplier's products are not listed.
P. Lejeune, et al.,
bioRxiv - Plant Biology 2020
Quote:
... Jiffypots® with weak or abnormal plantlets were discarded and the others were transplanted into 12-cm square plastic cultivation pots filled with 1.5 L of leaf mould and baked clay (4:1) mixed with 6 gr.L−1 of slow release fertilizer (Osmocote Exact Standard 5-6 M, ICL Specialty Fertilizers). The pots were fitted at the bottom with a 2 x 10 cm felt wick and randomly placed on the deck of the cultivation gutters described above ...
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No products found
because this supplier's products are not listed.
Helmut Bischof, et al.,
bioRxiv - Cancer Biology 2023
Quote:
Glucose uptake was assessed using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2- NBDG) (Biomol GmbH). Therefore ...
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No products found
because this supplier's products are not listed.
Michèle Reindl, et al.,
bioRxiv - Microbiology 2020
Quote:
... Quantitative Gus and LacZ liquid assays were based on the chromogenic and fluorescent substrates ONPG for the liquid LacZ activity assay (o-nitrophenyl-β-D-galactopyranoside) and MUG for the liquid Gus activity assay (4-methylumbelliferyl-ß-D-glucuronide trihydrate; BioWorld, 30350000-2 (714331), respectively (Stock et al. ...
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No products found
because this supplier's products are not listed.
Stephen J. DeCamp, et al.,
bioRxiv - Biophysics 2020
Quote:
Polymerized polyacrylamide gels were first treated with 440 μL of a 1:50 (v/v) mixture of Sulfosuccinimidyl 6-(4'-azido-2'-nitrophenylamino)hexanoate (Sulfo-SANPAH) (ProteoChem) and 50 mM HEPES solution under a UV light for 10 minutes ...
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No products found
because this supplier's products are not listed.
Shaohe Wang, Kazue Matsumoto, Kenneth M. Yamada,
bioRxiv - Developmental Biology 2020
Quote:
... 4 mL 5× PEG reagent (System Biosciences, LV825A-1) was added and mixed by pipetting ...
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No products found
because this supplier's products are not listed.
Surya Prakash Rao Batta, et al.,
bioRxiv - Cell Biology 2022
Quote:
HUVECs (passage 2 to 6; PromoCell) were routinely cultured in EBM media supplemented with the provided growth factors kit (Promocell) ...
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No products found
because this supplier's products are not listed.
Dong Hun Lee, et al.,
bioRxiv - Physiology 2022
Quote:
... HAPECs (passage 2-6, ScienCell, Carlsbad, CA) were incubated with 0.1 ng/mL interleukin-1 beta (IL-1β) ...
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No products found
because this supplier's products are not listed.
Annekatrien Boel, et al.,
bioRxiv - Genetics 2019
Quote:
... 1 hour and 4 hours of stimulation with TGFβ1 (4342-5, Biovision). Cells were scraped in PBS supplemented with protease inhibitor cocktail (1:100 ...
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No products found
because this supplier's products are not listed.
Seiya Yamada, et al.,
bioRxiv - Neuroscience 2021
Quote:
... were blocked for 2 h at 4°C with 5% normal goat serum in PBST and incubated with anti-EGFP (1:2000, Aves Labs, GFP-1010), anti-HA (1:500 ...
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No products found
because this supplier's products are not listed.
Aya Ghassani, et al.,
bioRxiv - Microbiology 2023
Quote:
... VII-1-1 and IX-5-2 (Genomic DNA extraction kit, Macherey-Nagel, Hoerdt) by using the primers listed in Table S4 ...
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No products found
because this supplier's products are not listed.
Anne Bourdais, Benoit Dehapiot, Guillaume Halet,
bioRxiv - Cell Biology 2021
Quote:
... then individually transferred into small (5 µl) drops of fixative (5:1:4 of methanol:acetic acid:water) on a glass-bottom dish (Mattek). After the zona pellucida had gradually dissolved ...
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No products found
because this supplier's products are not listed.
Agustin A. Corbat, et al.,
bioRxiv - Systems Biology 2021
Quote:
... 1% L-glutamine and 1% non-essential amino-acids (all PAN Biotech) at 37 °C and 5% CO2 in a humidified incubator ...
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No products found
because this supplier's products are not listed.
Alexandros Sfikas, et al.,
bioRxiv - Cell Biology 2019
Quote:
... siRNA 2 5’ GCCCUAUCCCUUUACGUCA (Eurogentec). Dharmacon ONTarget plus SMARTpool ...
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No products found
because this supplier's products are not listed.
Elizabeth Haythorne, et al.,
bioRxiv - Physiology 2019
Quote:
... mice were placed on diet at 5-6 weeks of age for 2 weeks (58% [wt/wt] fat and sucrose content; Research Diets, Inc) prior to analysis.
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No products found
because this supplier's products are not listed.
Brian T. Do, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... with a 4:2:1:1 ratio of the target:pMDLg:pMD2.G:pRSV-REV plasmids using Transit 293T reagent (Mirus). After 48 hours ...
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No products found
because this supplier's products are not listed.
Wenjing Zhang, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The membranes were then incubated with the following primary antibodies at 4°C for 6 h: GAPDH (1:6000, no. A19056), vinculin (1:1000, A2752) (ABclonal Technology, Co., China), caspase-3 (1:1000 ...
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No products found
because this supplier's products are not listed.
Shogo Tsujikawa, et al.,
bioRxiv - Neuroscience 2022
Quote:
... IL-6 (RayBiotech, ELM-IL6-1), PGE2 (Cayman Chemical ...
-
No products found
because this supplier's products are not listed.
Sonali Gupta, et al.,
bioRxiv - Systems Biology 2020
Quote:
... The amino acid solutions consisted of either EZ Amino Acids (AA, Teknova) or a modified amino acid solution (AA* ...
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No products found
because this supplier's products are not listed.
Maria Zimmermann-Kogadeeva, et al.,
bioRxiv - Systems Biology 2022
Quote:
... and lysed in a bead beater high for 4 min (2 min / kept on ice 1 min / 2 min) (BioSpec Products). Cellular debris was removed by centrifugation (8,000 × g ...
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No products found
because this supplier's products are not listed.
Arne H. Smits, et al.,
bioRxiv - Bioengineering 2019
Quote:
... Antibody 1: Rabbit anti-BRD4 amino acids 1312-1362 (Bethyl Laboratories, #A301985A). Antibody 2 ...
-
No products found
because this supplier's products are not listed.
Carlos A. Aldrete, et al.,
bioRxiv - Synthetic Biology 2024
Quote:
... and 1× MEM non-essential amino acids (Genesee, catalog no. 25-536). HEKBlue™ IL-2 ...
-
No products found
because this supplier's products are not listed.
Yohan S.S. Auguste, et al.,
bioRxiv - Neuroscience 2022
Quote:
... subcutaneous] before being anesthetized using isoflurane (SomnoSuite, Kent Scientific; 3-5% induction, 1-2% maintenance). Once anesthetic depth was achieved ...
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No products found
because this supplier's products are not listed.
Jordan J. Aoyama, Medha Raina, Gisela Storz,
bioRxiv - Microbiology 2021
Quote:
... was separated on denaturing 8% polyacrylamide gels containing 6 M urea (1:4 mix of Ureagel Complete to Ureagel-8 (National Diagnostics) with 0.08% ammonium persulfate ...
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6 micro-well glass bottom plate with #1 cover glass(0.13-0.16mm), micro-well size 14mm, with...
Cat# P06-14-1-N,
20/case, $178.00
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Laura R Lee, et al.,
bioRxiv - Plant Biology 2023
Quote:
... 5 mL of 1/2 MS with 2% low melt agarose was cast into imaging cuvettes (CellVis product number #C1-1.5H-N) after being filtered through a 0.45 micron nylon filter to remove any particulates that might disturb the path of the light sheet to prepare media “blankets” ...
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No products found
because this supplier's products are not listed.
Joonyong Lee, et al.,
bioRxiv - Physiology 2021
Quote:
... Serum IGF2 levels were quantified using a 1/6 dilution with the Mouse IGF-2 ELISA Kit (Boster Biological Technology, EK0381) as per directions by the manufacturer and read with the SpectraMax M2e spectrophotometer using the SoftMax Pro 6 program.
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No products found
because this supplier's products are not listed.
Timothy N. Hoang, et al.,
bioRxiv - Immunology 2020
Quote:
... The sections were incubated with Thyroid Transcription Factor-1 (Clone 8G7G3/1) for overnight at 4°C followed by a detection polymer system (MACH 2™; Biocare Medical). Labeled antibody was visualized by development of the chromogen (DAB Chromogen Kits ...
-
No products found
because this supplier's products are not listed.
Maria Font Farre, et al.,
bioRxiv - Plant Biology 2023
Quote:
... membranes were blocked in TBS-T/milk 5% overnight at 4°C and incubated in 1:2000 anti-PR2 (Agrisera, AS122366) in TBS-T/milk 5% for 1.5h at room temperature ...
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No products found
because this supplier's products are not listed.
Monika Chodasiewicz, et al.,
bioRxiv - Plant Biology 2022
Quote:
... Thermal unfolding of G-actin (2 μM) and F-actin (2 μM) was performed with the Tycho NT.6 (Nanotemper, Munich, Germany) according to the manufacturer’s instructions in G-buffer (5 mM Tris-HCl ...
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No products found
because this supplier's products are not listed.
Anthony Santella, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Rectangles of silicon wafer approximately 2×4 cm (Ted Pella, No 16015) were cut from the round plate glow discharged ...
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No products found
because this supplier's products are not listed.
Jun Tominaga, et al.,
bioRxiv - Plant Biology 2020
Quote:
... a large 6 x 6 cm chamber (6800-13; LI-COR) was used to maximize measurement precision ...
-
No products found
because this supplier's products are not listed.
Bridget Shafit-Zagardo, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Perilipin 2 (PLIN2) antibody recognizes the N-terminal amino acids 1-29 (Progen GP40, 1: 400). APC/CC1 (OP80 Millipore ...
-
No products found
because this supplier's products are not listed.
Reza Nejat, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... Ubiquitin−7-amino-4-methylcourmarin (Ub-AMC) was purchased from Boston Biochem; human ISG15−7-amino-4-methylcourmarin (ISG15-AMC ...
-
No products found
because this supplier's products are not listed.
Preksha Gupta, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Rainbow Calibration particles (RCP-60-5-2, RCP-60-5-5) were obtained from Spherotech. FITC and PE stained beads were part of the three color BD CaliBRITE kit (BD Biosciences 340486) ...
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No products found
because this supplier's products are not listed.
Maximilian W. G. Schneider, et al.,
bioRxiv - Cell Biology 2021
Quote:
... was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 μl of 5 mg ml−1 Biotin-XX-labeled tubulin (Pursolutions ...