-
No products found
because this supplier's products are not listed.
Bianca Dietrich, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... inhibition of NOTCH signalling in P1 organoids was achieved by adding either 50 µM [(2R,4R,5S)-2-benzyl-5-(Boc-amino)-4-hydroxy-6-phenyl-hexanoyl]-Leu-Phe-NH2 trifluoroacetate salt (L-685,458; Bachem) or 10 µM Deshydroxy LY-411575 (DBZ ...
-
No products found
because this supplier's products are not listed.
Nathalia L. Malacco, et al.,
bioRxiv - Immunology 2023
Quote:
Female B6 mice at 6-8 weeks old were provided with 3% w/v DSS (MP Biomedicals) in drinking water ad libitum for 5 days ...
-
No products found
because this supplier's products are not listed.
Alex Chialastri, et al.,
bioRxiv - Genomics 2022
Quote:
... One layer of photoresist (Microchem, SU-8 2075) is spun onto a silicon wafer at a thickness of 100 μm ...
-
No products found
because this supplier's products are not listed.
Mai Takenaka, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... riluzole (supply name: 2-amino-6-(trifluoromethyl)benzothiazole) and 4-chloro-3-ethylphenol (4-CEP) from Tokyo Chemical Industry (Tokyo, Japan), and 4-chloro-m-cresol (4-CMC ...
-
No products found
because this supplier's products are not listed.
Yeon Soo Kim, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... shSIRT3_#4: 5’-TCACATTCTGTTGACTCTCCATACTCAGC-3’) in pRS vector (Origene, TR309432) were used to stably transfect OVCA433 cells (Supp ...
-
No products found
because this supplier's products are not listed.
Martina Oravcová, et al.,
bioRxiv - Cell Biology 2022
Quote:
... arginine-6/lysine-8 (heavy, Cambridge Isotope Laboratories ...
-
No products found
because this supplier's products are not listed.
Aidan B Estelle, et al.,
bioRxiv - Biophysics 2023
Quote:
... either labeled with 5’ 6-carboxyfluorescein or unlabeled and reverse complement 14mer (5’-GACGUCCAUGUGCC-3’) were purchased from GenScript. The dsRNA was prepared by annealing the ss-14mer (5’-GGCACAUGGACGUC-3’ ...
-
No products found
because this supplier's products are not listed.
Coralie Hérent, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Cy-3 or Cy-5 (1:500, Jackson ImmunoResearch). Sections were counterstained with a fluorescent Nissl stain (NeuroTrace 435/445 blue ...
-
No products found
because this supplier's products are not listed.
Swati Gupta, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and Western Blotting samples were denatured at 94°C for 5 minutes in 3 x SDS sample buffer (150mM Tris pH 8, 6% SDS, 0.3M DTT ...
-
No products found
because this supplier's products are not listed.
Agata Szuba, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 5 mM dithiothreitol (DTT)) at 4°C overnight and concentrated to ~3-5 mg/mL using Vivaspin 6 concentrators (Sartorius, 6 ml, 100 kDa cut-off). Mammalian septin hexamers composed of mouse Sept2 (98.6% identical to human Sept2 ...
-
No products found
because this supplier's products are not listed.
Laura Gámez-Díaz, et al.,
bioRxiv - Immunology 2022
Quote:
... HEK293T cells were seeded as 0.3*106 cells in 6-wells plate followed by treatment for 4 h with 333 nM of Torin 1 (InvivoGen) alone or in combination with 200 nM of Bafilomycin A1 for 1 h.
-
No products found
because this supplier's products are not listed.
Han Zhu, et al.,
bioRxiv - Cell Biology 2022
Quote:
Samples were incubated on a rotator for 5 min at 4°C and then centrifuged at 500g for 5 min (Eppendorf, 5920R; 4°C, ramp speed of 3/3). Supernatant was removed and pellet was resuspended in sort buffer [1mM EDTA (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Lihong Wang-Bishop, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Mice in groups receiving αPD-1 and αCTLA-4 (100 µg, every 3 days for 5 injections, BioXcell, West Lebanon NH) were treated intraperitoneally ...
-
No products found
because this supplier's products are not listed.
Alexis M. Crockett, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Antibodies were diluted in 3% normal donkey serum (monoclonal mouse anti-mouse GFAP 1:2000, Sigma G-A-5; polyclonal rabbit anti-human IL-6 1:50, Proteintech), and incubated overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Ning Zhou, et al.,
bioRxiv - Plant Biology 2024
Quote:
... The infection phenotypes and events were determined at the indicated time-points by examining the roots stained with 5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside (X-Gal) using a light microscope (ECLIPSE Ni, Nikon, Tokyo, Japan).
-
No products found
because this supplier's products are not listed.
Qinyu Hao, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Oligonucleotides with 3’ amino group (LGC Biosearch Technologies) were pooled and coupled with either Cy®3 Mono NHS Ester (GE healthcare ...
-
No products found
because this supplier's products are not listed.
Walden Li, Ryan M. Wyllie, Paul A. Jensen,
bioRxiv - Microbiology 2020
Quote:
... Strains carrying the pLacZ plasmid appear blue when plated on 0.008% X-gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Zymo Research, CA, USA). X-gal concentrations above 0.008% appear to inhibit growth of S ...
-
No products found
because this supplier's products are not listed.
Kevin O. Saunders, et al.,
bioRxiv - Immunology 2020
Quote:
... One-methylpseudouridine (m1Ψ)-5’-triphosphate (TriLink) instead of UTP was used to generate modified nucleoside-containing mRNA ...
-
No products found
because this supplier's products are not listed.
Amédée Renand, et al.,
bioRxiv - Immunology 2020
Quote:
... sequences [SLA (p1-p53)] or 0.6 nmol/mL PepTivatorR Candida albicans MP65 (peptides pools of 15 amino acids length with 11 amino acid overlap, Miltenyi Biotec) in 5% human serum RPMI medium in the presence of 1µg/ml anti-CD40 (HB14 ...
-
No products found
because this supplier's products are not listed.
David S Uygun, et al.,
bioRxiv - Neuroscience 2021
Quote:
... sIPSCs were recorded at −70 mV in the presence of the glutamate receptor antagonists (20 μM 6-cyano-7-nitroquinoxaline-2,3-dione +50 μM D-(2R)-amino-5-phosphonopentanoic acid) using a Multiclamp 700B amplifier and pClamp 10.0 software (Molecular devices; California, United States). A 1 min period after 5 min application of the glutamate receptor antagonists was used for statistical analysis (Igor software ...
-
No products found
because this supplier's products are not listed.
Susan Hromada, et al.,
bioRxiv - Systems Biology 2021
Quote:
Genomic DNA was quantified using Sybr Green fluorescence assay with a 6-point DNA standard curve (0, 0.5, 1, 2, 4, 6 ng/μL Biotium). 1 μL of samples and 5 μL of standards were diluted into 95 μL of 1X SYBR Green (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Vikram Jakkamsetti, et al.,
bioRxiv - Neuroscience 2023
Quote:
We used a standard rotarod (rod diameter 3 cm, fall height 20 cm, start speed 4 rpm, acceleration rate 1 rpm/ 8 s, Touchscreen RotaRod, Harvard Apparatus) in several consecutive testing trials performed over 7 days while blinded to genotype and simultaneously testing control littermate mice ...
-
No products found
because this supplier's products are not listed.
Patricia Vit, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 2,2,3,3-d(4)-3- (trimethylsilyl)propionic acid sodium salt (TSP) by Alfa Aesar (Karlsruhe, Germany), potassium dihydrogen orthophosphate (KH2PO4) ...
-
No products found
because this supplier's products are not listed.
Charles R. Heller, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 1 - 4 tungsten micro-electrodes (FHC, 1-5 MΩ) were inserted to characterize the tuning and response latency of the region of cortex ...
-
No products found
because this supplier's products are not listed.
Anna V. Protasio, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Worms were rinsed in PBS (3×10mins, 3 × 1hr) and equilibriated in mounting media with 4’,6-diamidino-2-phenylindole DAPI (Fluoromount G, Southern Biotech, Birmingham, AL) overnight before mounting and imaging.
-
Chromatographically purified. A lyophilized powder.
Cat# LS006311,
Bulk, Inquire
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Kwangdeok Shin, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... Zebrafish ventricles were fixed in 3% PFA for 5 min and incubated in PBS supplemented with 1 mg/mL collagenase type 4 (Worthington Biochemical) at 4°C overnight ...
-
No products found
because this supplier's products are not listed.
Etienne Lelièvre, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... engMutfwd 5’-AGAACAGACGAATCTACAGCCGAA-3’ and engintron2rev 5’-AGCATGTTTTAACAAGACGGCAG-3’ primers and HotGoldStar PCR mix (Eurogentec).
-
No products found
because this supplier's products are not listed.
Antonio Marino, et al.,
bioRxiv - Systems Biology 2023
Quote:
... 4 3 2.0 mm SecurityGuard (Phenomenex) cartridge as a guard column ...
-
No products found
because this supplier's products are not listed.
Anna L. Vlasits, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Boroscillate pipettes (Sutter Instruments, 3-5 MΩ) were used for all recordings and whole-cell electrophysiology recordings were performed using a Multiclamp 700B amplifier (Molecular devices) ...
-
No products found
because this supplier's products are not listed.
Gemma L. Pearson, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Sections were then incubated in the following primary antisera overnight at 4°C in 1 X PBS + 1% tween + 20% Blocking One solution: rabbit anti-Aldh1a3 (Novus Biologicals; Littleton, CO, USA). Sections were then washed twice with PBS and incubated for 2 h at room temperature with species specific Cy2 and Cy3 conjugated secondary antibodies ...
-
No products found
because this supplier's products are not listed.
Luke A Johnson, et al.,
bioRxiv - Neuroscience 2020
Quote:
... In all patients a directional “1-3-3-1” electrode was used (4/5 patients: Abbott Infinity model 6172 ...
-
No products found
because this supplier's products are not listed.
Matteo Tassinari, et al.,
bioRxiv - Microbiology 2020
Quote:
... 1μL was then spotted on LB media supplemented with 40 μg/mL of 5-bromo-4-chloro-3-indolyl-β-D-galactoside (X-gal) (Euromedex), ampicillin (Amp ...
-
No products found
because this supplier's products are not listed.
Trisha A. Macrae, Miguel Ramalho-Santos,
bioRxiv - Molecular Biology 2020
Quote:
... 1 mM EGTA) and rotated 4h-overnight with 1 µg Rb anti-Usp9x (Bethyl), 1.7 µg Rb anti-Ezh2 (CST #5246) ...
-
No products found
because this supplier's products are not listed.
Yue Qu, et al.,
bioRxiv - Neuroscience 2021
Quote:
The completed constructs in M-6-attB-UAS-1-3-4 vector were amplified in Epi300 competent cells (EpiCentre) in LB-Chloramphenicol medium ...
-
No products found
because this supplier's products are not listed.
Francesco Roncato, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Images were acquired at a rate of one frame every 4-5 min for 4 h using an IX83 inverted microscope (Olympus) equipped with UPlanFLN 20x/0.50 Ph1 ∞/0.17/FN 26.5 objective (Olympus) ...
-
No products found
because this supplier's products are not listed.
Justin M. Westerfield, et al.,
bioRxiv - Biophysics 2021
Quote:
... the peptides were labeled at their N-terminus with an amine-reactive environmentally sensitive fluorescent reporter, Succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoate (NBD-X, SE) (AnaSpec, Fremont, CA) [37] ...
-
No products found
because this supplier's products are not listed.
Xinxin Zhang, et al.,
bioRxiv - Genetics 2019
Quote:
... Other mRNA fragments were incubated for 4h at 4°C with 3ug anti-m6A polyclonal antibody (Synaptic systems, 202003), which has combined with protein-A beads (Invitrogen ...
-
No products found
because this supplier's products are not listed.
J. Christopher Rounds, et al.,
bioRxiv - Neuroscience 2021
Quote:
... sonicated for 3×5 minutes in a 4°C Bioruptor ultrasonicator (UCD-200, Diagenode), vortexed ...
-
No products found
because this supplier's products are not listed.
Vasco Rodrigues, et al.,
bioRxiv - Cell Biology 2022
Quote:
Viral particles were produced by transfection of 293FT cells in 6-well plates with 3 μg of total DNA and 8 mL TransIT-293 Transfection Reagent (Mirus Bio) per well ...
-
No products found
because this supplier's products are not listed.
Melisa Martinez-Paniagua, et al.,
bioRxiv - Immunology 2022
Quote:
... Arrays were imaged for 6-8 hrs at an interval of 5 minutes using an Axio fluorescent microscope (Carl Zeiss) utilizing a 20x 0.45 NA objective ...
-
No products found
because this supplier's products are not listed.
Trinovita Andraini, et al.,
bioRxiv - Neuroscience 2021
Quote:
... 3-(2,4-Dichloro-5-methoxyphenyl)-2-sulfanyl-4(3H)-quinazolinone (BML-CM127-0050, Enzo Life Sciences) was injected at 20mg/kg (in 10% DMSO ...
-
No products found
because this supplier's products are not listed.
Shaibu Oricha Bello, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... Neutral red (3-amino-7-dimethylamino-2-methyl-phenazine hydrochloride) (Solarbio, cat. N8160), Minimal Essential Medium/Earls Balance Salts (MEM/EBSS ...
-
No products found
because this supplier's products are not listed.
Michelle E. Poling, et al.,
bioRxiv - Physiology 2022
Quote:
... cells were seeded on poly-D-lysine coated 8-well chambered culture slides for fixed imaging (MatTek CCS-8). The T7 flasks ...
-
No products found
because this supplier's products are not listed.
Thivanka Muthumalage, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... and 6 hours using microplate spectrophotometer (Cytation 5, Biotek).
-
No products found
because this supplier's products are not listed.
Md Gulam Musawwir Khan, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Cell survival was measured using the WST-8 (water-soluble Tetrazolium-8: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)- 5- (2,4-disulfophenyl)-2H-tetrazolium) assay kit (CCK-8; Dojindo Molecular Technologies, #CK04) following manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Gabriela Souza Barbosa, et al.,
bioRxiv - Physiology 2023
Quote:
Male and female C57Bl/6 mice (6-8 weeks of age) were divided into 4 groups and fed either a normal diet (ND; Research Diets, D12328 ...
-
No products found
because this supplier's products are not listed.
Rosalba Perrone, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... in 3-5% Bovine Serum Albumin - Fraction V in-1X TBS for one hour (Rockland, Baltimore, MD, BSA-1000). Block was removed and slides were incubated in the respective primary antibody diluted in protein block solution for 1 hour or overnight in a humidified chamber ...
-
No products found
because this supplier's products are not listed.
Syed Usman Enam, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and labeled on the 3’-end with amino-11-12 ddUTP (Lumiprobe A5040) using deoxynucleotidyl transferase (TdT ...
-
No products found
because this supplier's products are not listed.
Yan Chen, et al.,
bioRxiv - Molecular Biology 2022
Quote:
The purified complex samples (3 μL at 5-8 mg/mL) were applied to glow-discharged holey grids (Quantifoil R1.2/1.3, 300 mesh) and subsequently vitrified using a Vitrobot Mark IV (ThermoFisher ...
-
8 Well Chambered Cover Glass with #1 cover glass (0.13-0.16mm), with lid, sterilized. Designed...
Cat# C8-1-N,
48/case, $219.00
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Lissenya B. Argueta, et al.,
bioRxiv - Cell Biology 2021
Quote:
... hESC-qualified)-coated plates at 4×10^5/well in 24-well plates or 3×10^4/well in glass-like polymer bottom 96-well plates (CellVis).