-
No products found
because this supplier's products are not listed.
Mathieu Paquette, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... The beads were washed in PBS and the proteins eluted by incubating for 15 min with 50 mM Tris-HCl (pH 8.0) and 10 mM glutathione (Bioshop Canada, GTH001.5).
-
No products found
because this supplier's products are not listed.
Khushali Patel, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... ELISAs were performed using a β-hCG ELISA kit (EIA-1911; DRG International Springfield NJ) and IL-1β ELISA kit (DY401-05 ...
-
No products found
because this supplier's products are not listed.
Ria Göttert, et al.,
bioRxiv - Neuroscience 2020
Quote:
... The converted dye was solubilized in 10 %SDS in 0.01 M HCl and measured at 550 nm with a platereader (TriStar LB941, Berthold Technologies).
-
No products found
because this supplier's products are not listed.
Tomohide Saio, et al.,
bioRxiv - Biochemistry 2024
Quote:
... The reaction was quenched with an equivalent volume of 1 M HCl at the indicated time points and separated by RP-HPLC on a TSKgel Protein C4-300 column (Tosoh Bioscience, Japan) with monitoring at 229 nm ...
-
No products found
because this supplier's products are not listed.
Kevin R. Coffey, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Tissue was stained in a 5 % potassium ferrocyanide and 10 % HCl and counterstained for antibodies against calbindin D28k immunohistochemistry (ImmunoStar, Inc., Hudson WI), as described previously(Coffey et al. ...
-
No products found
because this supplier's products are not listed.
Miguel Ricardo Leung, et al.,
bioRxiv - Cell Biology 2022
Quote:
... anti-SPACA9 (HPA022243 from Atlas Antibodies, used at 6 μg/mL), or no primary antibody diluted in blocking buffer for 2 h at RT ...
-
No products found
because this supplier's products are not listed.
Zhouyi Rong, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Mouse Interleukin 6 (IL6) ELISA Kit (RD-IL6-Mu, Reddot biotech), Mouse Interferon Gamma Induced Protein 10kDa (IP10 ...
-
No products found
because this supplier's products are not listed.
M.C. van Zwam, et al.,
bioRxiv - Cell Biology 2022
Quote:
Parental and/or ALFA β-actin HT1080 cells were seeded in WillCo wells (WillCo Wells B.V.). The next day ...
-
No products found
because this supplier's products are not listed.
Bryce K. Allen, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... STING-mediated induction IFN-β in PBMCs from cynomolgus monkey (IQ Biosciences; donor 5032, Lot P19D2206) was tested after treatment for 6h using an ELISA (VeriKine Cynomolgus IFNb ELISA ...
-
No products found
because this supplier's products are not listed.
Ruhi Patel, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... plated on solid NGM supplemented with 8 mM isopropyl β-D-1-thiogalactopyranoside (IPTG, Laguna Scientific), and incubated for 16 to 24 h at 25° C ...
-
No products found
because this supplier's products are not listed.
Gudrun Liebscher, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Snap- frozen samples of iBAT were homogenized in NP40-free lysis buffer (0.5 mM DTT in 50 mM Tris-HCl (pH 8.0) and freshly added protease and phosphatase inhibitors) using a tapered tissue grinder (DWK Life Sciences, Milville, New Jersey). The homogenate was passed through a 25G needle 6 times and centrifuged for 10 min at 1,000 x g and 4°C (pellet ...
-
No products found
because this supplier's products are not listed.
Felix M. Bennetts, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
... Saturation assays involved the addition of increasing concentrations of [3H]-α,β-methylene ATP (Moravek Inc., USA). Competition assays utilized a sub-saturation concentration of [3H]-α,β-meATP (50 nM) ...
-
No products found
because this supplier's products are not listed.
Alex M. Eddie, et al.,
bioRxiv - Immunology 2021
Quote:
... in a 6-well cell culture plate (Peak Serum Inc. Cat. #TR5000), supplemented with recombinant mouse BAFF (10 ng/mL) ...
-
No products found
because this supplier's products are not listed.
Kuohan Li, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 10% glycerol and 5 mM β-mercaptoethanol and lysed by a Panda Plus 2000 homogenizer (GEA Niro Soavi). The lysate was clarified by high-speed centrifugation at 20 000×g at 4°C for 40 min ...
-
No products found
because this supplier's products are not listed.
Petra Pjevac, et al.,
bioRxiv - Microbiology 2019
Quote:
... Sample aliquots were transferred to 6 ml exetainer screw cap vials (Labco Limited) directly from the Niskin bottles ...
-
No products found
because this supplier's products are not listed.
Frøydis Sved Skottvoll, et al.,
bioRxiv - Biochemistry 2020
Quote:
... 6-MAM-d6 and morphine-d3 were purchased from Cerilliant (Austin, TX, USA). Unless otherwise stated ...
-
No products found
because this supplier's products are not listed.
Shabnam Ghiasvand, et al.,
bioRxiv - Neuroscience 2022
Quote:
6-well tissue culture plates were transferred to an interface chamber (Bioscience Tools) connected to a temperature controller maintaining temperature at 37 °C and a blood gas providing 5% CO2 ...
-
No products found
because this supplier's products are not listed.
Carole Y. Perrot, et al.,
bioRxiv - Immunology 2023
Quote:
... immersed in a pH=6 antigen retrieval solution (IHC World, Elliott City, MD) and placed in a steamer for 40 min at 95-98°C ...
-
No products found
because this supplier's products are not listed.
Hao Wang, et al.,
bioRxiv - Biochemistry 2024
Quote:
The gel was prepared with a concentration of 6% (wt/vol) agarose (HydraGene Co. ...
-
No products found
because this supplier's products are not listed.
Sandra Rizk, et al.,
bioRxiv - Microbiology 2020
Quote:
... For the differentiation between pigments with C30 and C40 carbon backbones Zeaxanthin and β-carotene (DHI Lab Products, Denmark) were run as examples for C40 pigments ...
-
No products found
because this supplier's products are not listed.
Xiao Lin, et al.,
bioRxiv - Plant Biology 2020
Quote:
A BAC library of plant GIG362-6 was generated by Bio S&T (Canada). A BAC clone that spans the mapping interval was isolated using molecular markers (Table S2 ...
-
No products found
because this supplier's products are not listed.
Jennifer McDonald, Catherine J. Merrick,
bioRxiv - Microbiology 2021
Quote:
Mature schizont cultures at >6% parasitaemia were synchronised using 55% Nycodenz (Alere technologies AS). Cultures were centrifuged and media removed to leave 2ml of media and blood ...
-
No products found
because this supplier's products are not listed.
Rupa Banerjee, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 6-9 PE-units) and 3-5 mg Silane-PEG-Biotin (Nanocs, 3400 Da) in a solution of 50 mL toluene at 55 °C ...
-
No products found
because this supplier's products are not listed.
Esther Sasson, et al.,
bioRxiv - Cell Biology 2021
Quote:
... and 1% β mercaptoethanol (Barna et al., 2016; Dempsey, Vaughan, Chen, Bates, & Zhuang, 2011; J. Zhang, Carver, Choveau, & Shapiro, 2016). A Nikon Ti-E inverted microscope was used ...
-
No products found
because this supplier's products are not listed.
Mathew Clement, et al.,
bioRxiv - Immunology 2020
Quote:
... The α and β chains of CD8 were cloned similarly into a single pSF-Lenti-EFα lentiviral vector (Oxford Genetics) separated by an IRES sequence (Genewiz) ...
-
No products found
because this supplier's products are not listed.
Anurag Pandey, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Anaesthetised mice were secured in a Narishige SR-6 stereotaxic frame (Narishige International, London, UK); the skull was thinned over a 2 x 2mm area above the barrel-field centerd on the D1 barrel (at approximately 3mm lateral to the midline and 1.5 mm caudal to bregma) ...
-
No products found
because this supplier's products are not listed.
Trung Q. Tran, et al.,
bioRxiv - Genomics 2020
Quote:
... 50 mM Tris at pH 7.4) and 14 μL of β-ME and 0.5 ml of a 10 mg/mL solution of zymolyase (Sunrise Science Products) prepared in buffer Z were added ...
-
No products found
because this supplier's products are not listed.
E.H. Bowler-Barnett, et al.,
bioRxiv - Cancer Biology 2021
Quote:
HCT116-GSK3β-KO and paired isogenic control cell line HCT116-GSK3β-WT were created by integrating a ssDNA oligonucleotide containing several stop codons at the GSK3 β locus with TALEN technology (Cellectis). Cell lines were cultured in McCoys 5A culture medium (Gibco ...
-
No products found
because this supplier's products are not listed.
Vincent Tano, et al.,
bioRxiv - Neuroscience 2022
Quote:
Approximately 1-2 million cells were lysed using FARB buffer with β-mercaptoethanol and RNA was purified using the FavorPrep Blood/Cultured Cell Total RNA Mini kit (Favorgen) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Jason Wallach, et al.,
bioRxiv - Neuroscience 2023
Quote:
... either coelenterazine h for β-Arrestin2 recruitment BRET1 or coelenterazine 400a for Gq dissociation BRET2 (Prolume/Nanolight, 5 µM final concentration) and was added per well ...
-
No products found
because this supplier's products are not listed.
Cristian Polo Rivera, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Ascus walls were digested for 20 minutes with β-glucuronidase and spores were then separated using a dissection microscope (Singer Instruments). After growth on a YPD plate for two days at 30°C ...
-
No products found
because this supplier's products are not listed.
Arash Farhadi, et al.,
bioRxiv - Bioengineering 2019
Quote:
... cells cultured in 6-well plates were lysed with 400 µL of Solulyse-M (Genlantis Inc) per well for one hour at 4 °C ...
-
No products found
because this supplier's products are not listed.
Thomas Hoffmann, et al.,
bioRxiv - Genetics 2023
Quote:
... STAG1 was stained with Polink 2 Plus HRP rat NM detection system (GBI Labs #D46-6) according to manufacturer’s instructions using a recombinant rat monoclonal STAG1 antibody (Abcam ab241544 ...
-
No products found
because this supplier's products are not listed.
Xiaoyan Shi, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Monoclonal rabbit anti-GAPDH antibody with 1: 5000 dilution (proteintech) and Monoclonal rabbit anti-β-actin antibody with 1: 3000 dilution (SAB Signalway Antibody).
-
No products found
because this supplier's products are not listed.
Minhui Guan, et al.,
bioRxiv - Microbiology 2024
Quote:
... Two biotinylated glycan analogs (3’SLN: Neu5Acα2-3Galβ1-4GlcNAcβ and 6’SLN: Neu5Acα2-6Galβ1- 4GlcNAcβ) were purchased (GlycoTech, Gaithersburg, MD). Three biotinylated glycans Neu5Acα2- 3Galβ1-4(Fucα1-3)GlcNAcβ (sLeX) ...
-
No products found
because this supplier's products are not listed.
You Zhang, et al.,
bioRxiv - Microbiology 2022
Quote:
... The β-lactamase in LASVpp-BlaM and LASV-VLP-BlaM incorporation and cleavage were examined by Western blotting using anti-β-lactamase antibody (QED Bioscience Inc, CA, USA).
-
No products found
because this supplier's products are not listed.
Paola Benaglio, et al.,
bioRxiv - Genomics 2020
Quote:
Peripheral blood mononuclear cells (PBMCs) from 10 individuals (4 females and 6 males) were purchased from HemaCare (Northridge, CA) and profiled for snATAC using 10x Genomics Chromium Single Cell ATAC Solution ...
-
No products found
because this supplier's products are not listed.
Jiang-An Yin, et al.,
bioRxiv - Genomics 2023
Quote:
... Viral titers of the sgRNA libraries were determined by a 6-point dose response in 6-well plates by puromycin selection and determination of live and dead cells (T.spiezzo, Cellecta) or flow cytometry of RFP+ cells (Brunello ...
-
No products found
because this supplier's products are not listed.
Ryutaro Ariyoshi, et al.,
bioRxiv - Biochemistry 2023
Quote:
... and MTG mutant was purified with size exclusion chromatography using ProteoSEC-D 16/60 6-600 HR (Protein Ark) with PBS (pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Christina Strauch, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and nuclei were visualized using 4’,6-diamidino-2-phenylindole (DAPI) in mounting medium (SCR-038448; Dianova, Hamburg, Germany).
-
No products found
because this supplier's products are not listed.
Lama El Cheikh Hussein, et al.,
bioRxiv - Neuroscience 2021
Quote:
... tail-tip blood (6 µl) was collected from 4 mice at ZT7 and ZT12 to check corticosterone levels (ELISA kit From Assaypro).
-
No products found
because this supplier's products are not listed.
María Concepción Izquierdo, et al.,
bioRxiv - Physiology 2023
Quote:
... we used 200 μl of plasma on Superose 6 10/300 GL column (Amersham Pharmacia Biotech) and FC-204 fraction collector (Gilson). For sequential density ultracentrifugation ...
-
No products found
because this supplier's products are not listed.
David Jukam, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... Eggs were irradiated by placing the 6 cm petri dish in a dark chamber for 2 minutes under a 500 Watt Hg arc lamp (Oriel Instruments) producing a downward facing focused beam with diameter of ∼8 cm such that every egg in the dish was covered by the UV light ...
-
No products found
because this supplier's products are not listed.
Hélène Neyret-Kahn, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... The ends of the DNA fragments were ligated to double stranded barcoded DNA adapters (NEXTflex ChIP-Seq Barcodes - 6, #514120, Bioo Scientific) using T4 DNA Ligase ...
-
No products found
because this supplier's products are not listed.
Jessica L. Kelliher, et al.,
bioRxiv - Microbiology 2023
Quote:
... Endpoint kinase assays were performed by mixing 3 μg purified PrkA1- 338 with the indicated combinations of ∼3x molar excess of substrate to kinase (6 μg of the generic kinase substrate myelin basic protein [MBP; Novatein Biosciences, Woburn ...
-
No products found
because this supplier's products are not listed.
Grzegorz Maciag, et al.,
bioRxiv - Cell Biology 2024
Quote:
... After antibody staining the tissue was washed 6 times in PBS and before imaging the tissue was incubated in RapiClear (SUNJin Lab) for 30-60 minutes at room temperature ...
-
No products found
because this supplier's products are not listed.
Maciej M. Jankowski, et al.,
bioRxiv - Neuroscience 2021
Quote:
... the outer panels of every third section contain two nose-poke ports (Fig 2; a total of 12 ports in 6 sections, SPECIAL.090-SE v1.0, LaFayette-Campden Instruments, Loughborough, UK). One of the two ports in each section is connected to a liquid pump and the other to a pellet dispenser ...
-
No products found
because this supplier's products are not listed.
Dieter G. Müller, et al.,
bioRxiv - Evolutionary Biology 2021
Quote:
... Colchicine treatment was performed using a disk of filter paper of 6 mm diameter loaded with 1 mg of colchicine (Fluka, Honeywell Research Chemicals), which was placed in the centre of an agar plate filled with gametophyte material ...
-
No products found
because this supplier's products are not listed.
Rio Ikuta, Yuu Kakinohana, Shun Hamada,
bioRxiv - Neuroscience 2023
Quote:
... the nuclei were labeled with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/mL) and then coverslipped with a mounting medium (Fluoroshield Mounting Medium; ImmunoBioScience, CA, USA). Images were obtained using a fluorescence microscope (Eclipse E800 ...
-
No products found
because this supplier's products are not listed.
Giovanni S. Offeddu, et al.,
bioRxiv - Cancer Biology 2020
Quote:
MVNs were cultured using Vasculife Endothelial Medium (LL-0003, Lifeline) and pooled HUVECs (GFP-expressing, Angio-Proteomie, 6 million ml-1 after five passages) and nHLFs (Lonza ...