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No products found
because this supplier's products are not listed.
Natalie S. Al-Otaibi, et al.,
bioRxiv - Microbiology 2019
Quote:
... wild-type 81116 strain cell culture grown to OD600 = 5 was applied onto glow-discharged C-flat holey carbon films 2/2 200 mesh (EMS). A Leica EM GP (Leica ...
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No products found
because this supplier's products are not listed.
Jessica T. Stieglitz, James A. Van Deventer,
bioRxiv - Synthetic Biology 2021
Quote:
... (S)-2-amino-6-(((prop-2-yn-1-yloxy)carbonyl)amino)hexanoic acid (AstaTech), Nε-Boc-L-lysine (Chem-Impex International) ...
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No products found
because this supplier's products are not listed.
Jessica T. Stieglitz, James A. Van Deventer,
bioRxiv - Synthetic Biology 2021
Quote:
... (S)-2-amino-6-((2-azidoethoxy)carbonylamino)hexanoic acid (Iris Biotech), (S)-2-amino-6-(((prop-2-yn-1-yloxy)carbonyl)amino)hexanoic acid (AstaTech) ...
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No products found
because this supplier's products are not listed.
Mainak Bose, et al.,
bioRxiv - Cell Biology 2023
Quote:
... the IVT reaction was doped with 5-amino-allyl UTP (Biotium) at 1:10 (aminoallyl-UTP ...
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No products found
because this supplier's products are not listed.
Caterina Di Sano, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... 1% nonessential amino acids and 2□mM L‐glutamine (all from Euroclone) was used for culturing A549 and Colo 699.The cells were maintained in an incubator at 37□°C with a humidified atmosphere with 5% CO2and were maintained as adherent monolayers ...
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No products found
because this supplier's products are not listed.
Francesco Limone, et al.,
bioRxiv - Neuroscience 2023
Quote:
... non-essential amino acids (NEAA, Stemcell technologies). After 24h (day0) ...
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No products found
because this supplier's products are not listed.
Zoe L. Watson, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... and 0.5 mM 2’-NH2-ATP (2’-amino-2’-deoxyadenosine-5’-triphosphate, purchased from Axxora). Reactions were incubated at 37 °C for 2 h ...
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No products found
because this supplier's products are not listed.
Darach Miller, Adam Dziulko, Sasha Levy,
bioRxiv - Systems Biology 2024
Quote:
Amino-acid additive stocks and selective plates with 5-FOA (GoldBio), hygromycin ...
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No products found
because this supplier's products are not listed.
Holly Melland, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), DL- 2-Amino-5-phosphonopentanoic acid (AP5), and Bafilomycin A1 were distributed by Sapphire Bioscience (Sydney, Australia) from Enzo Life Sciences (Lausen, Switzerland), Cayman Chemical (Ann Arbor ...
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No products found
because this supplier's products are not listed.
Fengrong Wang, et al.,
bioRxiv - Microbiology 2023
Quote:
... 2 mM DTT) using a 5-KD concentrator (Sartorius). The purified samples were aliquoted ...
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No products found
because this supplier's products are not listed.
Kirsty J. McMillan, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 2-5 MΩ borosilicate glass electrodes (GC150-10F, Harvard Apparatus) filled with aCSF were placed into the stratum radiatum of a GFP positive region of CA1 and a bipolar stimulating electrode (CBAPB50 ...
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No products found
because this supplier's products are not listed.
Justin M. Westerfield, et al.,
bioRxiv - Biophysics 2021
Quote:
... the peptides were labeled at their N-terminus with an amine-reactive environmentally sensitive fluorescent reporter, Succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoate (NBD-X, SE) (AnaSpec, Fremont, CA) [37] ...
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No products found
because this supplier's products are not listed.
Adele Xu, et al.,
bioRxiv - Genetics 2022
Quote:
... 5-ethynyl-2’-deoxyuridine (EdU) (Click Chemistry Tools 1381, Scottsdale, AZ) was added to a final concentration of 10 uM ...
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No products found
because this supplier's products are not listed.
Guillaume Etter, et al.,
bioRxiv - Neuroscience 2020
Quote:
... one adult mouse (~2 months) was anesthetized with isoflurane (5% induction, 0.5-2% maintenance) and placed in a stereotaxic frame (Stoelting). The skull was completely cleared of all connective tissue ...
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No products found
because this supplier's products are not listed.
Marius Weismehl, et al.,
bioRxiv - Biochemistry 2023
Quote:
Farnesylated GBP1 reconstituted on liposomes (as described above) were supplemented with 5 nm colloidal gold and applied on glow-discharged UltrAuFoil R 2/2 gold grids (Quantifoil Micro Tools), flash-frozen in liquid ethane using a Vitrobot Mark II device (FEI) ...
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No products found
because this supplier's products are not listed.
Karen. M. Marshall, et al.,
bioRxiv - Bioengineering 2023
Quote:
... the scaffolds seeded with C2C12 cells were moved to a 24 well plate and 1.5 mL of media was added (basal with 2% FCS ± 5 µg of BMP-2 (3.33 µg/mL BMP-2). Culture was continued for 4 days and ALP staining performed on the 5th day from cell seeding ...
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No products found
because this supplier's products are not listed.
Zhimin Huang, et al.,
bioRxiv - Bioengineering 2023
Quote:
... (2) block sections in 5% normal goat serum (SouthernBiotech) and 0.3% Triton-X solution in PBS for 1h at room temperature ...
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No products found
because this supplier's products are not listed.
Shreyas Krishnan, Richard L. Cryberg,
bioRxiv - Genetics 2019
Quote:
... 5 μL FailSafe 2× PCR premix G (EpiCentre, Madison, Wisconsin), approximately 25 ng genomic DNA template ...
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No products found
because this supplier's products are not listed.
Thi Tuyet Trinh Phan, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... MTT (3-(4,5-dimethylthiazol-2-yl-2, 5-diphenyl tetrazolium bromide)) was purchased from Alfa Aesar (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Shinya Ito, et al.,
bioRxiv - Neuroscience 2020
Quote:
We used 2–5 month-old CBA/CaJ (The Jackson Laboratory, 000654) mice of each sex ...
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No products found
because this supplier's products are not listed.
Anthony R. Anzell, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... 5% CO2 in endothelial growth medium 2 (EGM2, consisting of endothelial basal medium-2 supplemented with growth factors; Promocell) and 1X antibiotic-antimycotic (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Rayan Khaddaj-Mallat, et al.,
bioRxiv - Neuroscience 2021
Quote:
Pericytes viability was assessed using the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay according to the manufacturer’s procedure (Cell Signaling Technology). Cells were plated at the appropriate concentration in 96-well plates under different experimental conditions ...
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No products found
because this supplier's products are not listed.
Ana Domingo-Muelas, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 2+5 DIV differentiation samples were imaged using a Nikon ECLIPSE Ni-U microscope (Nikon) with a Zyla 4.2 sCMOS camera (Andor) ...
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No products found
because this supplier's products are not listed.
Artyom A. Egorov, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... complete amino acid supplementation) overnight in 96-well flat bottom plates (Eppendorf), diluted into fresh medium with a dilution factor of 200 ...
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No products found
because this supplier's products are not listed.
Patsy R. Tomlinson, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Cells were incubated at 2°C for 5 days and bacmid YFP expression assessed by Leica DM IL LED Fluo microscope using a green fluorescent protein filter cube ...
-
No products found
because this supplier's products are not listed.
Eric L. Van Nostrand, et al.,
bioRxiv - Genomics 2020
Quote:
... A 5’-amino modifier (Glen Research) was coupled onto the end of sequences for subsequent conjugation with the polymer ...
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No products found
because this supplier's products are not listed.
Andrew R Harris, et al.,
bioRxiv - Biophysics 2020
Quote:
... and 5% biotinyl-amino-PEG (Rapp Polymere, #13 3000-25-20) which was incubated for a minimum of 4 hours at 50°C ...
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No products found
because this supplier's products are not listed.
Alexandros Sfikas, et al.,
bioRxiv - Cell Biology 2019
Quote:
... siRNA 2 5’ GCCCUAUCCCUUUACGUCA (Eurogentec). Dharmacon ONTarget plus SMARTpool ...
-
No products found
because this supplier's products are not listed.
Victor Ruiz-Rodado, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... and A18110101 (L-amino acid diet with 2 g L-cysteine and 2 g L-cystine per kg) from Research Diets Inc ...
-
No products found
because this supplier's products are not listed.
Sonali Gupta, et al.,
bioRxiv - Systems Biology 2020
Quote:
... The amino acid solutions consisted of either EZ Amino Acids (AA, Teknova) or a modified amino acid solution (AA* ...
-
No products found
because this supplier's products are not listed.
Preksha Gupta, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Rainbow Calibration particles (RCP-60-5-2, RCP-60-5-5) were obtained from Spherotech. FITC and PE stained beads were part of the three color BD CaliBRITE kit (BD Biosciences 340486) ...
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No products found
because this supplier's products are not listed.
Elie M. Teyssonniere, et al.,
bioRxiv - Systems Biology 2023
Quote:
... The strains were grown in liquid SC medium (Yeast Nitrogen Base with ammonium sulfate 6.7 g.l−1, MPbio, OH, USA; amino acid mixture 2 g.l−1, MPbio; glucose 20 g.l−1, Euromedex, France). The culture was maintained until the strains reached their growth mid log phase using an optical plate reader (Tecan infinite F200 pro) ...
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No products found
because this supplier's products are not listed.
Samuel Hume, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... and using 5 μg anti-NUCKS1 antibody (ProteinTech 12023-2-AP) or 5 μg normal rabbit IgG (SantaCruz sc2027) ...
-
No products found
because this supplier's products are not listed.
Dillon S. McDevitt, et al.,
bioRxiv - Neuroscience 2019
Quote:
... recording electrodes (2-5 MΩ; borosilicate glass capillaries (WPI #1B150F-4) pulled on a horizontal puller from Sutter Instruments (model P-97) ...
-
No products found
because this supplier's products are not listed.
Shristi Baral, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Borosilicate glass pipettes (Sutter Instrument BF100-58-10, 2-5 MΩ) were pulled using a laser micropipette puller (Sutter Instrument Model P-2000) ...
-
No products found
because this supplier's products are not listed.
David S Uygun, et al.,
bioRxiv - Neuroscience 2021
Quote:
... sIPSCs were recorded at −70 mV in the presence of the glutamate receptor antagonists (20 μM 6-cyano-7-nitroquinoxaline-2,3-dione +50 μM D-(2R)-amino-5-phosphonopentanoic acid) using a Multiclamp 700B amplifier and pClamp 10.0 software (Molecular devices; California, United States). A 1 min period after 5 min application of the glutamate receptor antagonists was used for statistical analysis (Igor software ...
-
No products found
because this supplier's products are not listed.
Manuele Rebsamen, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Starvation media lacking a specific amino acid were prepared by complementing amino acid-free DMEM media (ie devoid of all 15 amino acids normally present, custom made by PAN biotech) with the other 14 amino acids (from individual powders ...
-
No products found
because this supplier's products are not listed.
Daniel R. Lu, et al.,
bioRxiv - Immunology 2019
Quote:
... with 5 μg purified anti-mouse IL-2 antibody (JES6-1, BioXcell) for 30 min at 37°C (molar ratio for IL2:anti-IL-2 is 2:1) ...
-
No products found
because this supplier's products are not listed.
Allie Lipshutz, et al.,
bioRxiv - Neuroscience 2022
Quote:
... angle 0°) under a combined isoflurane anesthetic (2-5%, SomnoSuite, Kent Scientific) and meloxicam analgesic (3 mg/kg ...
-
No products found
because this supplier's products are not listed.
Marcus A Begley, et al.,
bioRxiv - Cell Biology 2020
Quote:
... supplemented with non-essential amino acids (Genesee 25-536), sodium pyruvate (Genesee 25-537) ...
-
No products found
because this supplier's products are not listed.
Jose Ernesto Canton-Josh, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 2-5 mW) delivered through a 60X water-immersion objective (Olympus, Tokyo, Japan) with CoolLED PE4000 widefield illumination (CoolLED Ltd. ...
-
No products found
because this supplier's products are not listed.
Sneha Damal Villivalam, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 600 ng of denatured DNA was incubated with 2 mg of 5-methylcytosine (5-mC) monoclonal antibody ([33D3] Diagenode, Cat No # C15200081) in IP buffer (0.1% SDS ...
-
No products found
because this supplier's products are not listed.
Amanuel Tafessu, et al.,
bioRxiv - Genomics 2022
Quote:
... 5 μg of antibody and 2 µg of spike-in antibody (Active Motif 61686) were bound to 50 μl protein A or protein G Dynabeads (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Hooi Ting Hu, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Cells (5 × 105) were seeded into a well of culture-insert 2 well (ibidi, 80209). The cells were then cultured for 7 days without replacement of the medium and visualized using crystal violet staining.
-
No products found
because this supplier's products are not listed.
Yoichi Araki, et al.,
bioRxiv - Neuroscience 2020
Quote:
... or Spinning disk confocal microscopes controlled by axiovision software (Carl Zeiss; Fig. 2, 3, and 5). Following 5-10 min of baseline recording ...
-
An aqueous solution with toluene added as a preservative.
Cat# LS002764,
5 mg, $178.00
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Luke R. Perreault, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Minced tissue underwent 3-5 serial digestions in collagenase type 2 (Worthington Biochemical Corp, Lakewood, NJ) in sterile PBS with 20 mM glucose ...
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No products found
because this supplier's products are not listed.
Andrew G. Shuparski, et al.,
bioRxiv - Immunology 2021
Quote:
... cells were incubated for 5 mins with 2% (vol/vol) normal mouse and normal rat serum (Jackson ImmunoResearch Laboratories), prior to staining with a 2X master mix containing fluorophore-labeled antibodies at pre-titrated concentrations in Brilliant Violet Staining Buffer (BD Biosciences) ...
-
No products found
because this supplier's products are not listed.
Yang Wang, et al.,
bioRxiv - Microbiology 2024
Quote:
... and IL-5 (Mabtech, 3391-4HPW-2) precoated ELISpot kits according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Melanie A. MacMullan, et al.,
bioRxiv - Immunology 2022
Quote:
... Beads were then reconstituted in 200 µL elution buffer 2 (EB2, 5% ACN, 0.3M NH4OH) and incubated for 1 hour on a ThermoMixer at 45°C ...
-
No products found
because this supplier's products are not listed.
Michihito Sasaki, et al.,
bioRxiv - Microbiology 2022
Quote:
... followed by permeabilization with 0.5% Triton X-100 in PBS for 5 min and staining with anti-SARS-CoV-2 nucleocapsid rabbit monoclonal antibody (GTX635679, GeneTex) in 25% Block Ace (KAC ...