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Building Block
Sold for research purposes only.
Cat# 1284.0, SKU# 1284-1000 mg,
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Isabel R.K. Kuebler, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... The MCH receptor antagonist 6-(4-Chloro-phenyl)-3-[3-methoxy-4-(2-pyrrolidin-1-yl-ethoxy)-phenyl]-3H-thieno[3,2-d]pyrimidin-4-one (GW803430) (Axon Medchem, Groningen, Netherlands) was freshly prepared the day of the treatment ...
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Takushi Shimomura, et al.,
bioRxiv - Biophysics 2022
Quote:
In PI(3, 5)P2-injection experiments, 5 mM PI(3, 5)P2–diC8 (Echelon Biosciences) was manually injected by positive pressure using the glass needle filled with the PI(3 ...
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No products found
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Tulsi Upadhyay, Vaibhav V Karekar, Ishu Saraogi,
bioRxiv - Biochemistry 2020
Quote:
... All GrpE variants were labeled with DACM (N-(7-dimethylamino-4-methylcoumarin-3-yl)maleimide) (AnaSpec) and DnaK was labeled with BODIPY-fluorescein-N-(2-aminoethyl)-maleimide (Invitrogen ...
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No products found
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Pranay Shah, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... 3 μM of CHIR99021 (Cambridge Bioscience, SM13-5) and 10 μM of Y-27632 ...
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No products found
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Yohan S.S. Auguste, et al.,
bioRxiv - Neuroscience 2022
Quote:
... subcutaneous] before being anesthetized using isoflurane (SomnoSuite, Kent Scientific; 3-5% induction, 1-2% maintenance). Once anesthetic depth was achieved ...
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No products found
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Subash Godar, et al.,
bioRxiv - Biophysics 2021
Quote:
... We detected the bound alkaline phosphatase labeled antibodies by incubating the membrane in BCIP/NBT (5-bromo, 4-chloro, 3- indolylphosphate/nitro-blue tetrazolium, AMRESCO, LLC.) substrate for 30–45 minutes ...
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No products found
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Bas W.A. Bögels, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide HCl (EDC, Carbosynth), 1,6-diaminohexane (Sigma ...
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No products found
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Tori Tonn, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... glass slides (4’’ by 3’’; Ted Pella) and the feature-side of the PDMS slabs were thoroughly cleaned with tape to remove any dust particles ...
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No products found
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Chun-Yu Fu, et al.,
bioRxiv - Biochemistry 2024
Quote:
... SSC was separated on a 250 x 3 mm 5 µm Nucleodur HTec C18 (Macherey-Nagel) column ...
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2-Oxo-3-phenylpropanoic acid (Phenylpyruvic acid) is used in the synthesis of 3-phenyllactic...
Cat# S6131, SKU# S6131-25mg,
25mg, $97.00
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Jeanne Corriveau, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... HUVECs were serum starved in M199 media with 0.5% FBS for 3 h followed by a 7 h treatment with 1 μM group I PAK inhibitor FRAX1036 (Selleck Chemicals).
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No products found
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Nicholas C. Bauer, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 3-5×104 cells were seeded into each well of a chambered 8-well 1.5H coverglass (Ibidi 80827) and allowed to adhere overnight prior to further manipulation ...
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No products found
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Prasath Paramasivam, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 5 µg pre-miR-181a-1 were labeled with cy3 using Nucleic Acid Labeling Kit (Mirus) following the manufacturer’s instructions.
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No products found
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Lei Chen, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... ATPase (MBL International Corp. D032-3, 1:200), P63 (Santa Cruz sc-8343 ...
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No products found
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Danielle M Paul, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Kinesore (3,5-dibromo-N′-[2,5-dimethyl-1-(3-nitrophenyl)-1H-pyrrol-3-yl]methylene}-4-hydroxybenzohydrazide) was obtained from Chembridge Corporation (Cat ...
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No products found
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Alvina I. Khamidullina, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Primers CDKN1B-forv 5’-attagctagcATGTCAAACGTGCGAGTGTCTAA-3’ and CDKN1B-rev 5’-taatggatccTTACGTTTGACGTCTTCTGAGGC-3’ (Evrogen, Moscow, Russia) containing NheI and BamHI restriction sites were used for amplification ...
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No products found
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Matthew D. Kerr, et al.,
bioRxiv - Bioengineering 2022
Quote:
... (4-(1,2,4,5-tetrzain-3-yl)phenyl)methanamine (tetrazine amine) was purchased from Kerafast (FCC659, lot: 2014). 1-bicyclo[2.2.1]hept-5-en-2-ylmethanamine (norbornene amine ...
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Watcharachai Meemetta, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Forward primer LCHV-MEP93-qF: 5’-GTACTTCATCGCCTACGGAGC-3’ and reverse primer LCHV-MEP93-qR: 5’-TACGTGTGCTTGAGGAGGTC-3’ were synthesized from Bio Basic, Canada ...
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No products found
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Thorsten M. Leucker, et al.,
bioRxiv - Cell Biology 2021
Quote:
... PCSK9 mRNA expression was assessed by real-time PCR using PCSK9 primer sets 5’-TGTCTTTGCCCAGAGCATC-3’ and 5’-GTCACTCTGTATGCTGGTGTC-3 (Integrated DNA Technologies) and SYBR Select Master Mix (ThermoFisher, ABI 4472908).
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No products found
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Alina Sultanova, et al.,
bioRxiv - Immunology 2020
Quote:
... 3 and 5 were obtained from Abnova (Taipei, Taiwan). The proteins were produced in vitro using wheat germ expression system which preserves native protein folding.
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No products found
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Saphala Dhital, Naren R. Vyavahare,
bioRxiv - Bioengineering 2019
Quote:
DiR (1, 1-dioctadecyl-3, 3, 3, 3-tetramethylindotricarbocyanine iodide) (PromoCell GmbH, Heidelberg, Germany) loaded BSA (Seracare ...
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No products found
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Andrew B. Stergachis, et al.,
bioRxiv - Genetics 2023
Quote:
... which preserves 3’ and 5’ end information (PacBio, Menlo Park, CA). A PacBio SMRTbell library was constructed using these PCR-amplified full-length cDNA transcripts and sequenced using a Sequel II ...
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No products found
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Michael D. Roberts, et al.,
bioRxiv - Physiology 2023
Quote:
Peptides obtained from each of the five NP mixtures were separately reconstituted according in a solution of 0.1% formic acid and 3% acetonitrile (34) spiked with 5 fmol μL PepCalMix from SCIEX (Framingham, MA, USA). Reconstitution volumes varied by NP types to allow for constant peptide quantity for MS injection between samples regardless of starting volume (240 ng ...
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Alina Ainbinder, et al.,
bioRxiv - Genetics 2021
Quote:
... An in vitro transcribed sgRNA targeting Slc16a11 (5’-AGTCCTAACCTCGCTTGGCT-3’) and hspCas9 mRNA (CAS500A-1, System Biosciences) were microinjected into C57BL/6J mouse zygotes by the Genome Modification Facility at Harvard University ...
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Kazuhiro Fukumura, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... hnRNPH1: 5′-CUCUUGUCCAUCUAGAC-3′] used for the NMR titration was purchased (IBA Lifesciences).
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Matteo Tassinari, et al.,
bioRxiv - Microbiology 2020
Quote:
... 1μL was then spotted on LB media supplemented with 40 μg/mL of 5-bromo-4-chloro-3-indolyl-β-D-galactoside (X-gal) (Euromedex), ampicillin (Amp ...
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Roman Sloutsky, et al.,
bioRxiv - Biochemistry 2020
Quote:
... blotted for 5 s and plunge-frozen into liquid ethane using Cryoplunge 3 (Gatan).
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Bohm Lee, et al.,
bioRxiv - Neuroscience 2021
Quote:
... which was crushed for 3 seconds with Dumont #5 forceps (Fine Science Tools, 11254-20) and special care was taken not to damage the vein sinus ...
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J. Michael Henderson, et al.,
bioRxiv - Cell Biology 2022
Quote:
Streptavidin-coated polystyrene beads (3 μm in diameter, SVP-30-5, 0.5% w/v, Spherotech) were washed three times in a 10X volume of PBS and recovered by centrifugation (12,000 rpm ...
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Hannah Wapenaar, et al.,
bioRxiv - Biochemistry 2023
Quote:
... and HRP conjugated secondary antibody, PonceauS (3% trichloroacetic acid, 3% sulfosalicylic Acid, 0.2% Ponceau) or stainfree gels (Mini-PROTEAN TGX Stain-Free Precast Gels).
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Aakash Basu, et al.,
bioRxiv - Neuroscience 2022
Quote:
... mice were first anaesthetized with isoflurane in oxygen (3-5% during induction, slowly lowered throughout the surgery to 1-2%) while placed in a stereotaxic apparatus (Stoelting). Eyes were lubricated with ophthalmic ointment ...
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Jennifer Schwarz, et al.,
bioRxiv - Biochemistry 2021
Quote:
... or with 0.5 µg/ml doxycycline for 2-3 days) were combined in a 1:1 ratio and loaded with 5 µg/ml Indo-1 (Molecular Probes, AAT Bioquest, Sunnyvale, USA) and 0.04% of pluronic F-127 (AAT Bioquest ...
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Anne Bourdais, Benoit Dehapiot, Guillaume Halet,
bioRxiv - Cell Biology 2021
Quote:
... then individually transferred into small (5 µl) drops of fixative (5:1:4 of methanol:acetic acid:water) on a glass-bottom dish (Mattek). After the zona pellucida had gradually dissolved ...
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S. Liu, et al.,
bioRxiv - Cell Biology 2023
Quote:
... containing 0.1% Triton X-100 and blocked 1 hour in 5% goat serum (Jackson Immuno Research, 005-000-121) 3% BSA (Pan Biotech, P06-1391100). Rabbit anti-phospho-ubiquitin ...
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No products found
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Ana S Almeida, et al.,
bioRxiv - Microbiology 2021
Quote:
... and three 3–4 mm sterile glass beads (Biospec Products). Next ...
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No products found
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Tiesuo Zhao, et al.,
bioRxiv - Immunology 2024
Quote:
... cleaved-caspase 3 (1:1000, Bioworld), Pro-Caspase 3 (1:2000 ...
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No products found
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Gonzalo Sanchez, et al.,
bioRxiv - Cell Biology 2021
Quote:
Detached MIN6 cells (3 to 5 million/ml) were transferred to a non-adherent petri dish (Sarstedt, Nümbrecht, Germany) with 5 ml culture medium and kept in culture for 5-7 days at 37 °C in an humidified atmosphere with 5% CO2 until they spontaneously formed cellular aggregates (pseudoislets).
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Hyeogsun Kwon, et al.,
bioRxiv - Immunology 2021
Quote:
Phagocytosis assays were performed by injecting 69 nl of 2% green fluorescent FluoSpheres (vol/vol) in 1X PBS to naïve female mosquitoes (3-to 5-day old) using a Nanoject II injector (Drummond Scientific). After injection ...
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No products found
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Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... IP of total SUMOylated protein fractions used either SUMO-1 or SUMO-2/3 Ab included with the Signal Seeker SUMOylation 1 or 2/3 Detection Kit (Cytoskeleton) and IPs were carried out according to the manufacturer instructions ...
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No products found
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Jamie Guenthoer, et al.,
bioRxiv - Immunology 2024
Quote:
... Omicron BA.4/BA.5 (Sino Biological, cat. 40589-V08H32), Omicron XBB (Sino Biological ...
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No products found
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Yuchen Li, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Organoid sections (4 µm) were probed with anti-cleaved caspase 3 (Biocare Medical) in 10% goat serum for 2 hours at room temperature and subsequently stained with Mach 1 Universal Polymer HRP ...
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No products found
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Chunfeng Mao, Maria Mills,
bioRxiv - Biophysics 2023
Quote:
... 0.25 mM PMSF) and eluted with 4 × 1 mL 0.15 mg/mL (50 µM) 3 x FLAG peptide (Chromotek). The elutant was concentrated with Amico-4 (50k MWCO ...
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No products found
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Raviprasad Kuthethur, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... active + pro Caspase-3 (1:3000) (ABclonal, Wuhan, China), B-Actin (1:5000 ...
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No products found
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Joanna X. Campbell, et al.,
bioRxiv - Microbiology 2022
Quote:
... which was synthesized by incorporating Fmoc-beta-(7- methoxycoumarin-4-yl)-Ala-OH (Mca, Bachem) at position 10 and Fmoc-Cys(Trt)-OH (Novabiochem ...
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No products found
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Jun Sun, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Rabbit anti-5-HT 1:500 (Immunostar); Rabbit Anti-DCP-1 1:250 (Cell Signalling) ...
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No products found
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Stephen B. McHugh, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Sections were then incubated with primary antibodies diluted in 3% NDS blocking solution and incubated at 4 °C for 72 hours (GFP anti-chicken, 1:1,000, Aves Labs, catalog no ...
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No products found
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Lucia Gonzalo, et al.,
bioRxiv - Plant Biology 2021
Quote:
... and 1/1000 of Histone 3 (H3 AS10 710, Agrisera) or RNAPII (AS11 1804 ...
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No products found
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Valerie Betting, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... RNA was transferred to Hybond Nx nylon membranes (Amersham) and crosslinked using EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride) (Sigma) (Pall & Hamilton 2008). Membranes were pre-hybridized in Ultrahyb Oligo hybridization buffer (Invitrogen) ...
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No products found
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Jun Feng, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... The cultures were then inoculated into DM29-fructose (5 g L- 1) and DM29-glucose (5 g L-1) media in a 96-well plate (Greiner Bio-One) to an initial OD600 of 0.05 ...
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No products found
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Susanne Wiemann, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 3 % goat serum (Dianova, Hamburg, Germany), and 0.5 % Triton™-X-100 (Sigma-Aldrich ...
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No products found
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Bianca Maria Rotoli, et al.,
bioRxiv - Cell Biology 2019
Quote:
Calu-3 cells (American Type Culture Collection), obtained from a human lung adenocarcinoma and derived from serous cells of proximal bronchial airways ...