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Building Block
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Cat# 1135.0, SKU# 1135-1000 mg,
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Isabel R.K. Kuebler, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... The MCH receptor antagonist 6-(4-Chloro-phenyl)-3-[3-methoxy-4-(2-pyrrolidin-1-yl-ethoxy)-phenyl]-3H-thieno[3,2-d]pyrimidin-4-one (GW803430) (Axon Medchem, Groningen, Netherlands) was freshly prepared the day of the treatment ...
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No products found
because this supplier's products are not listed.
Thomas W. Jackson, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... and 2H,2H,3H,3H-Perfluorooctane-1-sulfonate (6:2 FTSA, CAS 59587-39-2, purity ≥ 97%) were from Synquest Laboratories (Alachua, FL). HSA (CAS 70024-90-7 ...
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No products found
because this supplier's products are not listed.
Sytse J. Piersma, et al.,
bioRxiv - Immunology 2020
Quote:
... and host Actb (Forward: 5’-AGCTCATTGTAGAAGGTGTGG-3’; Reverse: 5’ - GGTGGGAATGGGTCAGAAG-3’; Probe: 5’-TTCAGGGTCAGGATACCTCTCTTGCT-3’; IDT DNA) against plasmid standard curves using TAQman universal master mix II on a StepOnePlus real time PCR system (Thermo Fisher Scientific).
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No products found
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Tracy J. Berg, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Primary human astrocytes (3H Biomedical) were cultured in Astrocyte Medium (3H Biomedical ...
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No products found
because this supplier's products are not listed.
Anushka Nayak, et al.,
bioRxiv - Biochemistry 2023
Quote:
... and tri-methyl lysine (Abclonal). Anti-GAPDH-HRP was used to measure even loading.
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No products found
because this supplier's products are not listed.
Karen Gu, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... This cDNA library was then PCR barcoded with PCR Primer 1.0 (5’-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG-3’) and a sample-specific barcode primer (5’-CAAGCAGAAGACGGCATACGAGATNNNNNNGTGACTGGAGTTCAGACGTG-3’) using MyTaq Red Mix (Bioline) for 15 cycles ...
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No products found
because this supplier's products are not listed.
Pranay Shah, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... 3 μM of CHIR99021 (Cambridge Bioscience, SM13-5) and 10 μM of Y-27632 ...
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No products found
because this supplier's products are not listed.
Karen. M. Marshall, et al.,
bioRxiv - Bioengineering 2023
Quote:
... the scaffolds seeded with C2C12 cells were moved to a 24 well plate and 1.5 mL of media was added (basal with 2% FCS ± 5 µg of BMP-2 (3.33 µg/mL BMP-2). Culture was continued for 4 days and ALP staining performed on the 5th day from cell seeding ...
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No products found
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Caleb R. Carr, et al.,
bioRxiv - Microbiology 2024
Quote:
... 2 µL of 10 µM 3’ PacBio round 2 reverse primer (PacBio_3pri_RND2), and 25 µL KOD Hot Start Master Mix ...
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No products found
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Ulrika Simone Spangsberg Petersen, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... All SSOs used are RNA oligonucleotides with 2’-O-methyl modifications and phosphorothioate backbones (LGC Biosearch Technologies; Risskov, Denmark) (Table S2) ...
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No products found
because this supplier's products are not listed.
Görkem Garipler, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... and 2-inhibitor cocktail (3 mM CHIR (BioVision) and 1 mM PD0325901 (Sigma) ...
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No products found
because this supplier's products are not listed.
Anderson R Frank, et al.,
bioRxiv - Cancer Biology 2022
Quote:
All lentiviruses were produced by co-transfection of HEK 293T/17 cells with plasmid DNA for the respective lentiviral vector and the packaging components psPAX2 and pMD2.G at a 5:3:2 mass ratio (vector:psPAX2:pMD2.G) using TransIT-LT1 (Mirus MIR 2300). Lentiviral supernatants were collected at 48 and 72 hours post-transfection ...
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No products found
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Huntly M. Morrison, et al.,
bioRxiv - Immunology 2022
Quote:
... Sections were stained with 0.3% uranyl acetate/2% methyl cellulose and viewed on a JEOL 1200 EX transmission electron microscope (JEOL USA) equipped with an AMT 8 megapixel digital camera and AMT Image Capture Engine V602 software (Advanced Microscopy Techniques) ...
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No products found
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Yang Wang, et al.,
bioRxiv - Microbiology 2024
Quote:
... and IL-5 (Mabtech, 3391-4HPW-2) precoated ELISpot kits according to the manufacturer’s instructions ...
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No products found
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Yusuke Nishimura, et al.,
bioRxiv - Cell Biology 2021
Quote:
Akt1/2/3 inhibitor MK-2206 dihydrochloride (ApexBio, A3010), Rapamycin (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Harold P. Hodgins, et al.,
bioRxiv - Genomics 2022
Quote:
... VAMP1/2/3 (104102) were purchased from Synaptic Systems. Antibody against actin (AC-15 ...
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No products found
because this supplier's products are not listed.
Samuel Hume, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... and using 5 μg anti-NUCKS1 antibody (ProteinTech 12023-2-AP) or 5 μg normal rabbit IgG (SantaCruz sc2027) ...
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No products found
because this supplier's products are not listed.
Oskar Staufer, et al.,
bioRxiv - Immunology 2022
Quote:
... cells were stained with anti-human ICAM-1-AlexaFluor647 (BioXcell, BE0020-2, 3 µM), anti-human CD58-AlexaFluor647 (BD Bioscience ...
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No products found
because this supplier's products are not listed.
Hongbing Zhang, et al.,
bioRxiv - Bioengineering 2020
Quote:
... 5 mg/mL SARS-CoV-2 spike protein (Sino biological, 40591-V08H) was coated on high binding plates (Corning ...
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No products found
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Dipsikha Biswas, et al.,
bioRxiv - Cell Biology 2020
Quote:
... and 0.8 ng/uL in H2O containing sodium-2-Keto-3-methyl-d3-butyrate-3,4,4,4d4 (KIVd7; CDN Isotopes), 120 µl of 6M perchloric acid (VWR ...
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No products found
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Kevin J. Forsberg, et al.,
bioRxiv - Microbiology 2021
Quote:
... 40% (+/-)-2-methyl-2,4-pentanediol (MPD, Hampton Research), 10mM MgCl2 as a mother liquor ...
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No products found
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Aidan McGlinchey, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 3β-Hydroxy-5-cholestene-3-linoleate (ChoE(18:2)) from Larodan, were prepared to the following concentration levels ...
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No products found
because this supplier's products are not listed.
Alvina I. Khamidullina, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Primers CDKN1B-forv 5’-attagctagcATGTCAAACGTGCGAGTGTCTAA-3’ and CDKN1B-rev 5’-taatggatccTTACGTTTGACGTCTTCTGAGGC-3’ (Evrogen, Moscow, Russia) containing NheI and BamHI restriction sites were used for amplification ...
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No products found
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Nina R. Montoya, et al.,
bioRxiv - Microbiology 2022
Quote:
... The plates were developed with TMB (3, 3’, 5, 5’ - Tetramethylbenzidine) ELISA Peroxidase Substrate (Rockland Immunochemical, Limerick, PA), and absorbance was measured on a plate reader at 655 nm.
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No products found
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Wenli Yang, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 8-(4-Chlorophenylthio)-2’-O- methyladenosine-3’,5’-cyclic monophosphate acetoxymethyl ester (007-AM) was from Axxora (Cat. # BLG-C051). DAPI (Cat ...
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No products found
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Jimin Yoon, et al.,
bioRxiv - Biophysics 2023
Quote:
... the cells were stained with 200 μL of 5 mg/mL solution of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) stain (Research Products International) in PBS ...
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No products found
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Thorsten M. Leucker, et al.,
bioRxiv - Cell Biology 2021
Quote:
... PCSK9 mRNA expression was assessed by real-time PCR using PCSK9 primer sets 5’-TGTCTTTGCCCAGAGCATC-3’ and 5’-GTCACTCTGTATGCTGGTGTC-3 (Integrated DNA Technologies) and SYBR Select Master Mix (ThermoFisher, ABI 4472908).
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No products found
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Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... IP of total SUMOylated protein fractions used either SUMO-1 or SUMO-2/3 Ab included with the Signal Seeker SUMOylation 1 or 2/3 Detection Kit (Cytoskeleton) and IPs were carried out according to the manufacturer instructions ...
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No products found
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Morgane Bomsel, et al.,
bioRxiv - Microbiology 2021
Quote:
... and exposed for 3h to 500nM or 5μM biotinylated CGRP (AnaSpec) in 100μl RPMI medium added to the apical side ...
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No products found
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Preksha Gupta, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Rainbow Calibration particles (RCP-60-5-2, RCP-60-5-5) were obtained from Spherotech. FITC and PE stained beads were part of the three color BD CaliBRITE kit (BD Biosciences 340486) ...
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No products found
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Jacob Kumro, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Methyl methacrylate powder (A-M Systems, Everett, WA) was mixed with its respective dental cement solvent until saturated and then a thin layer of the mix was applied to the top of the skull with the periosteal elevators ...
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No products found
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Timothy J. Ross-Elliott, et al.,
bioRxiv - Plant Biology 2019
Quote:
Methyl-β-cyclodextrin was purchased from Frontier Scientific, tyrphostin A51 and turanose were purchased from Sigma-Aldrich ...
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No products found
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Zijun Sun, Thomas C. Südhof,
bioRxiv - Neuroscience 2020
Quote:
... fetal bovine serum (ATLANTA Biological; final concentration = 2-3%) were added to the culture medium ...
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No products found
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Erin C. Bogacki, Patrick A. Lewis, Susanne Herbst,
bioRxiv - Cell Biology 2023
Quote:
L-leucyl-L-leucine methyl ester (LLOMe; BAChem, #4000725.0001) was stored at a stock concentration of 333 mM in ethanol and diluted to 1 mM in 10% FCS in DMEM for experimental use ...
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No products found
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Jennifer Schwarz, et al.,
bioRxiv - Biochemistry 2021
Quote:
... or with 0.5 µg/ml doxycycline for 2-3 days) were combined in a 1:1 ratio and loaded with 5 µg/ml Indo-1 (Molecular Probes, AAT Bioquest, Sunnyvale, USA) and 0.04% of pluronic F-127 (AAT Bioquest ...
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No products found
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Ziqiang Lin, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Sections were incubated in Histoclear (2×3 min; National Diagnostics, USA) and cover-slipped with mounting medium (Sigma Aldrich ...
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No products found
because this supplier's products are not listed.
Kazuhiro Fukumura, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... hnRNPH1: 5′-CUCUUGUCCAUCUAGAC-3′] used for the NMR titration was purchased (IBA Lifesciences).
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No products found
because this supplier's products are not listed.
Bohm Lee, et al.,
bioRxiv - Neuroscience 2021
Quote:
... which was crushed for 3 seconds with Dumont #5 forceps (Fine Science Tools, 11254-20) and special care was taken not to damage the vein sinus ...
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No products found
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S. P. Maher, et al.,
bioRxiv - Cell Biology 2023
Quote:
... vivax cases were infected into PHH lot BGW at day 2 post-seed (for case 1) or day 3 post-seed (for cases 2 and 3) in 384-well plates (Greiner Bio-One cat 781956) using the same methods for initiating P ...
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No products found
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Gaëlle Hogrel, et al.,
bioRxiv - Biochemistry 2022
Quote:
... These were diluted 2-fold with water and ultracentrifuged using 3 kDa MWCO spin filters (Pall). Liquid chromatography analysis was performed on the Dionex UltiMate 3000 system ...
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No products found
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Lasse Kvich, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... filled to ∼1/3 volume with 2 and 0.1 mm diameter zirconia beads (Biospec, OK, USA) on ice ...
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No products found
because this supplier's products are not listed.
Yongrong Liao, et al.,
bioRxiv - Cell Biology 2023
Quote:
... washed 3 times for 5 min in PBS and permeabilized with 0.1% Triton X-100 and 0.02% SDS (Euromedex, EU0660) in PBS for 5 min ...
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No products found
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Ghazal Vahidi, et al.,
bioRxiv - Physiology 2023
Quote:
... followed by Rayon fine cloths and alumina pastes (9, 5, 3, 1, 0.5, 0.3, and 0.05 μm, Ted Pella, Inc.).
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No products found
because this supplier's products are not listed.
Ming Zhou, et al.,
bioRxiv - Plant Biology 2021
Quote:
... (2) A ∼5 cm (15 µL capacity) glass capillary tube (Drummond Scientific, Cat# 1-1000-0150) was inserted in the 2 mm perforation such that the bottom of the tube reaches the 500 µl mark when the lid is refastened to the Eppendorf tube ...
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No products found
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Rowyn C Liebau, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Membranes were then blocked for 1-2 hours in 5% powdered milk (Amresco, M203-10G-10PK) in PBST ...
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No products found
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Nicole A. Jandick, Cathy L. Miller,
bioRxiv - Microbiology 2023
Quote:
... Membranes were washed with 1x TBST 3 times and incubated for 5 minutes 3 times between antibody incubations and before addition of NovaLume Atto Chemiluminescent Substrate AP (Novus Biologicals). Membranes were then imaged on a ChemiDoc SRS Imaging System (Bio-Rad Laboratories ...
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No products found
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Eleonora Grisard, et al.,
bioRxiv - Cell Biology 2021
Quote:
... rabbit anti-human 14-3-3 1/1000 (EPR6380, GeneTex), rabbit anti-human Lamp1 1/1000 (clone EPR4204 ...
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No products found
because this supplier's products are not listed.
Hannah Fitzgerald, et al.,
bioRxiv - Physiology 2023
Quote:
... and anti-Galectin 3 (Mac-2) (Cedarlane Cat# CL8942AP). For this co-stain ...
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No products found
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Roman Sloutsky, et al.,
bioRxiv - Biochemistry 2020
Quote:
... blotted for 5 s and plunge-frozen into liquid ethane using Cryoplunge 3 (Gatan).
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No products found
because this supplier's products are not listed.
Kerri L. Miazgowicz, et al.,
bioRxiv - Microbiology 2022
Quote:
... Transfected cells were rapidly cooled and stained in blocking solution (dPBS with 2% (v/v) bovine serum albumin (BSA)) containing anti-MXRA8 (1:100, W040-3, MBL International), anti-hTIM1(1:100 ...