-
No products found
because this supplier's products are not listed.
Jaime M. Pita, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... and 4 µM 5-fluoro-2′-deoxyuridine (FldU, Sigma-Aldrich) were added to the cells ...
-
No products found
because this supplier's products are not listed.
Diede de Haan, et al.,
bioRxiv - Cell Biology 2021
Quote:
... PDMPO [2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-amino-carbamoyl)methoxy)-phenyl)oxazole] (ThermoFisher Scientific, USA) was added to a final concentration of 330 µM ...
-
No products found
because this supplier's products are not listed.
Janne Purhonen, Jukka Kallijärvi,
bioRxiv - Molecular Biology 2023
Quote:
... BI stands for Broccoli aptamer ligand (5Z)-3-(1H-Benzimidazol-7-ylmethyl)-5-[(3,5-di-fluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-4H-imidazol-4-one dihydrochloride) (Cat. No. 7466, Tocris) (14) ...
-
No products found
because this supplier's products are not listed.
Chisato M. Yamazaki, et al.,
bioRxiv - Bioengineering 2020
Quote:
... and 1-methoxy-5-methylphenazinium methylsulfate (1-methoxy PMS, 100 μM, Cayman Chemical) was added to each well ...
-
No products found
because this supplier's products are not listed.
Lubna Amer, Maurice Retout, Jesse V. Jokerst,
bioRxiv - Bioengineering 2023
Quote:
The percent toxicity was calculated using an XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) colorimetric assay (Biotium) wherein viable cells form an orange formazan product upon reduction ...
-
No products found
because this supplier's products are not listed.
R. Wercberger, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 2% Fluoro-Gold (Fluorochrome), or the HSV-hEF1a-GFP-L10a.
-
No products found
because this supplier's products are not listed.
Hua Tian, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole) and coverslips were mounted over slides in Fluoro-Gel (EMS; 17985-10) and imaged by spinning disc confocal microscopy and Aperio Versa 8 fluorescent slide scanner.
-
No products found
because this supplier's products are not listed.
Amanda N.D. Adams, et al.,
bioRxiv - Microbiology 2021
Quote:
... 200µg/ml 5’-fluoro-2’-deoxyuridine (VWR), 100ng/ml anhydrotetracycline (Sigma) ...
-
No products found
because this supplier's products are not listed.
Maali AlAhmad, et al.,
bioRxiv - Cell Biology 2024
Quote:
... 4-hydroxy-3-methoxy-acetophenone (Apocynin, 73536, Merck), gp-91-ds-tat (AS-63818 ...
-
No products found
because this supplier's products are not listed.
Nitin Wadhwani, et al.,
bioRxiv - Cancer Biology 2022
Quote:
SJ-GBM2 and SF8628 cells were used to determine if reducing WDR82 through inducible knockdown affected cell viability 1×104 cells/100μl were plated in 96-well plates with complete cell culture medium with or without Dox (2μg/ml), and subjected to 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS, Promega) assay.
-
No products found
because this supplier's products are not listed.
Brian J. Thomas, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... 2’-fluoro modified CTP and 2’-fluoro modified UTP (TriLink Biotechnologies). All RNA aptamers were purified through denaturing polyacrylamide gel electrophoresis (0.75 mm ...
-
No products found
because this supplier's products are not listed.
Steven M. Wellman, et al.,
bioRxiv - Neuroscience 2023
Quote:
... mice were injected intraperitoneally with methoxy-X04 (2 mg/kg, Abcam, #ab142818) 24 hours prior to imaging20 ...
-
No products found
because this supplier's products are not listed.
S. ter Horst, et al.,
bioRxiv - Microbiology 2022
Quote:
2’-Fluoro-2’-deoxycytidine (2’-FdC) (Biosynth-Carbosynth) was dissolved in 100% DMSO (VWR Chemicals) ...
-
No products found
because this supplier's products are not listed.
Doyoung Kwon, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
... 3-[2-(N,N-diethyl-N-methylammonium)ethyl]-7-methoxy-4-methylcoumarin (AMMC, 100 μM; Cyp2d10; Cat. No. 451700, Corning Inc.), 7-methoxy-4-(trifluoromethyl)-coumarin (MFC ...
-
No products found
because this supplier's products are not listed.
Mélanie Rogier, et al.,
bioRxiv - Immunology 2020
Quote:
... IL-4 (5 ng/ml; Peprotech) and an anti-CD40 antibody (200 ng/ml ...
-
No products found
because this supplier's products are not listed.
Rayan Khaddaj-Mallat, et al.,
bioRxiv - Neuroscience 2021
Quote:
Pericytes viability was assessed using the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay according to the manufacturer’s procedure (Cell Signaling Technology). Cells were plated at the appropriate concentration in 96-well plates under different experimental conditions ...
-
No products found
because this supplier's products are not listed.
Dougald M. Monroe, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 4 μM lipid vesicles (Avanti Polar Lipids PS:PC:PE 3:2:5), and FV (0 ...
-
No products found
because this supplier's products are not listed.
Dan Xia, et al.,
bioRxiv - Neuroscience 2021
Quote:
... with 10 mg/kg methoxy-X04 (R&D Systems). Mice were perfused with PBS 24 hours after injection and cortical and hippocampal tissues were dissected and processed into single-cell suspension for staining as described above except the antibodies used for FACS were ...
-
No products found
because this supplier's products are not listed.
Sora Kim, et al.,
bioRxiv - Biophysics 2022
Quote:
... were mixed in 70 μL of assembly buffer (50 mM HEPES-KOH, pH 7.5, 300 mM KCl, 5 mM MgCl2, 2 mM TCEP, 4% glycerol, 2 mM ATPγS [Calbiochem]) for 5 min at 25 °C ...
-
No products found
because this supplier's products are not listed.
Caleb Ruiz-Jiménez, et al.,
bioRxiv - Immunology 2021
Quote:
... was incubated with 50μl MTT (sodium 3′-[1-(phenylaminocarbonyl)-3,4-tetrazolium]-bis (4-methoxy-6-nitro) benzene sulfonic acid hydrate) labeling reagent (Roche Life Science, USA) to each well ...
-
No products found
because this supplier's products are not listed.
Mareike D. Hoffmann, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
... 5 μl of the resulting amplicon were diluted 1:4 in 1x buffer 2 (NEB), followed by denaturation and re-annealing in a nexus GSX1 Mastercycler (Eppendorf ...
-
No products found
because this supplier's products are not listed.
Jun Liu, et al.,
bioRxiv - Immunology 2021
Quote:
... and stained with fluorochrome-labeled anti-mouse IFN-γ/TNF-α/IL-2/IL-4/IL-5 mAbs (Biolegend). LSRFortessa was used to acquire the flow cytometry data ...
-
No products found
because this supplier's products are not listed.
S. ter Horst, et al.,
bioRxiv - Microbiology 2022
Quote:
2’-Fluoro-2’-deoxycytidine (2’-FdC) (Biosynth-Carbosynth) was dissolved in 100% DMSO (VWR Chemicals) ...
-
No products found
because this supplier's products are not listed.
Shene Chiou, et al.,
bioRxiv - Cell Biology 2024
Quote:
... 5% v/v 2-mercaptoethanol) and fractionated by 4-15% Tris-Glycine gel (Bio-Rad Cat#5678084) using Tris-Glycine running buffer (0.2M Tris-HCl ...
-
No products found
because this supplier's products are not listed.
Liang Wang, et al.,
bioRxiv - Cell Biology 2023
Quote:
... #4: 5’-CCGGTTTAGCTGAAGATTCAA-3’ (SI00443779, Qiagen), GTPBP10 siRNA 5’-TTGCGTGTTGTTCAGAAAGTA-3’ (SI04308647 ...
-
No products found
because this supplier's products are not listed.
Christine Krammer, et al.,
bioRxiv - Immunology 2021
Quote:
... 8 color (4-2-2) configuration (BD Bioscience, Heidelberg, Germany). The system automatically adjusts spillover values for the compensation of standard fluorochromes ...
-
No products found
because this supplier's products are not listed.
Rohan Kodgule, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133; V5 tag, ThermoFisher #R960-25; ETV6, Santa Cruz, # sc-166835x ...
-
Cat# HY-W008378-10 mM * 1 mL,
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Guohui Zhang, et al.,
bioRxiv - Biophysics 2022
Quote:
BC5 (ARG-4-Methoxy-2-Naphthylamine, ordered from Medchemexpress LLC Monmouth Junction, NJ) was dissolved into DMSO to make 100 mM stock solution and then added to recording solutions to the final concentrations ...
-
No products found
because this supplier's products are not listed.
Dillon S. McDevitt, et al.,
bioRxiv - Neuroscience 2019
Quote:
... recording electrodes (2-5 MΩ; borosilicate glass capillaries (WPI #1B150F-4) pulled on a horizontal puller from Sutter Instruments (model P-97) ...
-
No products found
because this supplier's products are not listed.
Hengqian Ren, et al.,
bioRxiv - Biochemistry 2023
Quote:
... D-amino standards were generated by reacting the L-amino acid solution with 1-fluoro-2-4-dinitrophenyl-5-D-alanine amide (D-FDAA, Toronto Research Chemicals). These reaction products are enantiomers to the hypothetical D-amino acid and L-FDAA coupled products ...
-
No products found
because this supplier's products are not listed.
Niccolò Paolo Pampaloni, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Slices were superfused with recirculating aCSF (5 mL/min at room temperature) containing 4-methoxy-7-nitroindolinyl-glutamate (MNI-caged-L-Glutamate; HelloBio HB0423) at a concentration of 0.5 mM and used for the whole day of recordings.
-
No products found
because this supplier's products are not listed.
Joanna X. Campbell, et al.,
bioRxiv - Microbiology 2022
Quote:
... Purified and quantified Hist-5Mca was then reacted under basic conditions in a 1:1 molar ratio with 27.3 µM 4-fluoro-7-sulfamoylbenzofurazan (ABD-F, TCI America) in DMSO for 20 min over a 60 °C water bath ...
-
No products found
because this supplier's products are not listed.
Konstadinos Moissoglu, et al.,
bioRxiv - Cell Biology 2020
Quote:
... sgRNAs targeting RABIF (sg #2: 5’-GAGCGAGTTAGTGTCAGCCGAGG-3’ and sg #4 5’-AGCGAGTTAGTGTCAGCCGAGGG-3’) were cloned in lentiCRISPRv2 (Addgene #52961). MDA-MB-231 cells were infected with the corresponding lentiviruses and selected with 1μg/ml puromycin (Thermo Fisher Scientific).
-
No products found
because this supplier's products are not listed.
Martineau Éric, et al.,
bioRxiv - Neuroscience 2020
Quote:
... then 2h at RT with a rabbit IgG anti-S100β antibody (1:250; Dako-Agilent, cat# Z031101-2 or between 1:4 and 1:5, Dako-Agilent, cat#IR50461-2) followed by 2h at RT with the chicken IgY anti-Neurofilament medium chain (NF-M ...
-
No products found
because this supplier's products are not listed.
Chi-Yeh Chung, et al.,
bioRxiv - Genomics 2019
Quote:
... incubated for 5 min at 4 °C with rotation and pelleted again (500 x g, 5 min, 4°C; 5920R, Eppendorf). Nuclei were resuspended in 500 μL high salt tagmentation buffer (36.3 mM Tris-acetate (pH = 7.8) ...
-
No products found
because this supplier's products are not listed.
Chong Wang, et al.,
bioRxiv - Genetics 2022
Quote:
... the pre-implantation embryos were harvested from 4-5-week-old C57BL/6N females mated with DBA/2 males (8-week-old when purchased from Charles River). To be specific ...
-
No products found
because this supplier's products are not listed.
Azharul Islam, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 4′6-diamidino-2-phenylindole hydrochloride (Vector Laboratories). More than 20 randomly selected fields of view per sample were photographed using a WHN10×/22 eyepiece and a 60× objective (field of view is 1.1 mm ...
-
No products found
because this supplier's products are not listed.
G. Unali, et al.,
bioRxiv - Microbiology 2021
Quote:
... 2% glutamine and 5% human serum AB (Lonza) for seven days ...
-
No products found
because this supplier's products are not listed.
Ying Yu, et al.,
bioRxiv - Genetics 2022
Quote:
... reverse transcription was carried out using 2 μL of RNA mixed with 4 μL of 5×PrimeScript IV cDNA Synthesis Mix (Takara, Code No. 6215A) containing PrimeScript IV RTase ...
-
No products found
because this supplier's products are not listed.
Chris P. Sarnowski, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The resulting supernatant was incubated for 4-5 h at 4 °C on glutathione sepharose 4B (GE Healthcare) (3 mL per 2 L expression) ...
-
No products found
because this supplier's products are not listed.
Vishwaratn Asthana, et al.,
bioRxiv - Bioengineering 2020
Quote:
... 2’-fluoro (2’-F) modified RNA was synthesized using the DuraScribe T7 transcription kit (Lucigen) as described in the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Hajime Suyama, Veronica Egger, Michael Lukas,
bioRxiv - Neuroscience 2021
Quote:
... using DAPI fluoro mount-G (Southern Biotech, Birmingham, AL, USA).
-
No products found
because this supplier's products are not listed.
Paul Renauer, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 4 x 2 mm (Phenomenex). The eluents were A ...
-
No products found
because this supplier's products are not listed.
Jeroen van den Berg, et al.,
bioRxiv - Cell Biology 2019
Quote:
... a Lionheart FX automated microscope in combination with sirDNA77 staining was used to generate growth curves with a time resolution of 4 hours for a total time span of 136 hours (microscope maintained at 37°C, 5% CO2 using a 4× lens and a Sony CCD, 1.25 megapixel camera with 2 times binning; BioTek). Quantification of cell number was performed by Gen5 software (BioTek).
-
No products found
because this supplier's products are not listed.
Yan Shin J. Liao, et al.,
bioRxiv - Physiology 2019
Quote:
... containing 2-4% normal goat serum (Jackson Laboratories). Tissues were incubated with primary antibodies for 2 hours at 37°C ...
-
No products found
because this supplier's products are not listed.
Sayantan Roy, Bing Wang, Yuan Tian, Qian Yin,
bioRxiv - Biochemistry 2023
Quote:
... 5 mM MgCl2 and 2 mM Tris (2-carboxyethyl) phosphine (TCEP, GoldBio).
-
No products found
because this supplier's products are not listed.
Jessica Migliavacca, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Addgene)[70] in a ratio of 5:3:2 using polyethylenimine (24765-2, Polysciences). Virus supernatant was harvested 30 h after transfection ...
-
No products found
because this supplier's products are not listed.
David A. Siegel, et al.,
bioRxiv - Genomics 2022
Quote:
... and DRB3, 4, 5 (exon 2) genes with Fluidigm Access Array (Fluidigm, Singapore) and sequenced on an Illumina MiSeq sequencer (Illumina, San Diego, USA). HLA alleles and genotypes are called using the Omixon HLA Explore (version 2.0.0 ...
-
No products found
because this supplier's products are not listed.
Fatmanur Tiryaki, et al.,
bioRxiv - Cell Biology 2021
Quote:
... for 5 minutes at 4°C with TLA100 rotor (Beckman). 100 µg cell lysate was brought to a final volume of 250 µl BRB80 supplemented with 1 mM GTP ...
-
No products found
because this supplier's products are not listed.
Anthony Flamier, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and passaging every 4-5 days using ReLeSR (StemCell technologies). hES-qualified Matrigel (Corning ...