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No products found
because this supplier's products are not listed.
Zhexin Wang, et al.,
bioRxiv - Cell Biology 2020
Quote:
... and 3 times at 10,000 rpm for 5 s (IKA TC10 basic ULTRA-TURRAX® homogenizer with S10N-5G dispersing element ...
-
No products found
because this supplier's products are not listed.
Shanna H. Coop, Jacob L. Yates, Jude F Mitchell,
bioRxiv - Neuroscience 2022
Quote:
... We inserted tungsten 2.5- to 5-MΩ electrodes (1-3 FHC) that were mounted onto a lightweight screw micro-drive (Crist Instrument ...
-
No products found
because this supplier's products are not listed.
Ligia B. Schmitd, et al.,
bioRxiv - Neuroscience 2024
Quote:
... mice were anesthetized with isoflurane (5% induction, 2-3% maintenance, SomnoSuite Kent Scientific) 10d after the first SNC and the crush placed immediately proximal to the first one ...
-
No products found
because this supplier's products are not listed.
Yoichi Araki, et al.,
bioRxiv - Neuroscience 2020
Quote:
... or Spinning disk confocal microscopes controlled by axiovision software (Carl Zeiss; Fig. 2, 3, and 5). Following 5-10 min of baseline recording ...
-
No products found
because this supplier's products are not listed.
Chun-Yu Fu, et al.,
bioRxiv - Biochemistry 2024
Quote:
... SSC was separated on a 250 x 3 mm 5 µm Nucleodur HTec C18 (Macherey-Nagel) column ...
-
No products found
because this supplier's products are not listed.
Ghazal Vahidi, et al.,
bioRxiv - Physiology 2023
Quote:
... followed by Rayon fine cloths and alumina pastes (9, 5, 3, 1, 0.5, 0.3, and 0.05 μm, Ted Pella, Inc.).
-
Sodium 2-(1H-indol-3-yl)acetate (3-Indoleacetic acid sodium, Indole-3-acetic acid sodium, 3-IAA...
Cat# S3324, SKU# S3324-25mg,
25mg, $99.00
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Jeanne Corriveau, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... HUVECs were serum starved in M199 media with 0.5% FBS for 3 h followed by a 7 h treatment with 1 μM group I PAK inhibitor FRAX1036 (Selleck Chemicals).
-
No products found
because this supplier's products are not listed.
Kevin J. McNaught, et al.,
bioRxiv - Molecular Biology 2020
Quote:
H3K27me2/3 ChIP using anti-H3K27me2/3 antibody (Active Motif, 39536) was performed as previously described (12 ...
-
No products found
because this supplier's products are not listed.
Amin Zargar, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... 5-methyl-6-(propan-2-yl)oxan-2-one was synthesized by Enamine (Cincinnati, USA) to greater than 95% purity.
-
No products found
because this supplier's products are not listed.
Mary E. Law, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... cells were incubated with 0.5 mg/mL MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (Biomatik, Wilmington, DE USA) in PBS for 3 h ...
-
No products found
because this supplier's products are not listed.
Alvina I. Khamidullina, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Primers CDKN1B-forv 5’-attagctagcATGTCAAACGTGCGAGTGTCTAA-3’ and CDKN1B-rev 5’-taatggatccTTACGTTTGACGTCTTCTGAGGC-3’ (Evrogen, Moscow, Russia) containing NheI and BamHI restriction sites were used for amplification ...
-
No products found
because this supplier's products are not listed.
Jérémy Dufloo, et al.,
bioRxiv - Microbiology 2024
Quote:
... Correct insertion was checked by colony PCR using vector-specific primers (Forward: 5’-GAGAACCCACTGCTTACTGGC-3’; Reverse: 5’-AGGGTCAAGGAAGGCACG-3’) and the NZYTaq II 2x Green Master Mix (NZYtech). Plasmids with correct insertions were checked by Sanger (Eurofins ...
-
No products found
because this supplier's products are not listed.
Nicolò Mangraviti, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... The full cDNA sequence of the lncRNA was then amplified with forward primer: 5’- GTATCATAAGGATCCCTTTCCACTGCTCTGGTGAG-3’ and reverse primer: 5’- GTATCATAAGTCGACCTCACCTAGCTGTCTGTCC-3’ and cloned into pAAV-MCS (Cat#: VPK-410, Cell Biolabs Inc.) using the restriction enzymes BamH I and HindIII sites ...
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No products found
because this supplier's products are not listed.
Weronika Czaban, Jim Rasmussen,
bioRxiv - Plant Biology 2019
Quote:
... ground plant material was extracted in a 1-ml extraction mixture of chloroform, methanol and water (1:3:1, v:v:v) in Sarstedts Eppendorf tubes (Sarstedt AG & Co, Nümbrecht, Germany). One metal bead (a 3-mm tungsten carbide bead ...
-
No products found
because this supplier's products are not listed.
Katelyn A. Queen, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 24 hours after induction of GFP-KIF18A and endogenous KIF18A knockdown cells were fixed for 3 minutes in -20oC methanol and subsequently stained for rabbit-KIF18A 1:100 (Bethyl, A301-080A), guinea-pig- CENP-C (MBL ...
-
No products found
because this supplier's products are not listed.
Luke A Johnson, et al.,
bioRxiv - Neuroscience 2020
Quote:
... In all patients a directional “1-3-3-1” electrode was used (4/5 patients: Abbott Infinity model 6172 ...
-
No products found
because this supplier's products are not listed.
Rebecca A. MacPherson, et al.,
bioRxiv - Genomics 2022
Quote:
... We used 2μL of the resulting DNA mixture in a PCR reaction with primers (Left: 5’-CTAGCACGGAACCCTGGAAAT -3’; Right: 5’-GCAGCGCCTAGTAATCACAGA -3’) according to ApexRedTaq (Genesee Scientific, El Cajon, CA) manufacturer instructions ...
-
No products found
because this supplier's products are not listed.
Andrew B. Stergachis, et al.,
bioRxiv - Genetics 2023
Quote:
... which preserves 3’ and 5’ end information (PacBio, Menlo Park, CA). A PacBio SMRTbell library was constructed using these PCR-amplified full-length cDNA transcripts and sequenced using a Sequel II ...
-
No products found
because this supplier's products are not listed.
Joanna X. Campbell, et al.,
bioRxiv - Microbiology 2022
Quote:
... which was synthesized by incorporating Fmoc-beta-(7- methoxycoumarin-4-yl)-Ala-OH (Mca, Bachem) at position 10 and Fmoc-Cys(Trt)-OH (Novabiochem ...
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No products found
because this supplier's products are not listed.
Luke A. Simpson, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Caps were fixed with 1% methanol-free formaldehyde-PBS (Covaris) for 15 minutes before quenching ...
-
No products found
because this supplier's products are not listed.
Zhaoduan Liang, et al.,
bioRxiv - Immunology 2019
Quote:
... 5-Bromo-4-chloro-3-indolyl phosphate/Nitro blue tetrazolium (BCIP/NBT, Mabtech) was used to develop the immune-spot according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Kazuhiro Fukumura, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... hnRNPH1: 5′-CUCUUGUCCAUCUAGAC-3′] used for the NMR titration was purchased (IBA Lifesciences).
-
No products found
because this supplier's products are not listed.
Elena A. Andreeva, et al.,
bioRxiv - Biophysics 2022
Quote:
... the protein solution (∼ 5 ml) was circulated with a peristaltic pump (Gilson Minipuls 3) through a 2 mm X-ray quartz capillary in a closed loop ...
-
No products found
because this supplier's products are not listed.
Vivian K. Rojas, et al.,
bioRxiv - Microbiology 2024
Quote:
... The chromogenic molecule X-Phos (5-bromo-4-chloro-3-indolyl phosphate, Chem-Impex) was added to agar plates with the purpose of detecting S ...
-
No products found
because this supplier's products are not listed.
Bohm Lee, et al.,
bioRxiv - Neuroscience 2021
Quote:
... which was crushed for 3 seconds with Dumont #5 forceps (Fine Science Tools, 11254-20) and special care was taken not to damage the vein sinus ...
-
No products found
because this supplier's products are not listed.
J. Michael Henderson, et al.,
bioRxiv - Cell Biology 2022
Quote:
Streptavidin-coated polystyrene beads (3 μm in diameter, SVP-30-5, 0.5% w/v, Spherotech) were washed three times in a 10X volume of PBS and recovered by centrifugation (12,000 rpm ...
-
No products found
because this supplier's products are not listed.
Olivier Da Ines, et al.,
bioRxiv - Genetics 2022
Quote:
... 2 mM X-Gluc (5-bromo-4-chloro-3-indolyl-ß-D-glucuronic acid; Biosynth), dissolved in N,N-dimethylformamide) ...
-
No products found
because this supplier's products are not listed.
Subodh K. Mishra, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... permeabilized using 1:1 methanol/acetone and blocked using Background Sniper (Biocare Medical). Slides were incubated with 1:100 rabbit anti Clcn1 (Alpha Diagnostic International ...
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No products found
because this supplier's products are not listed.
Saphala Dhital, Naren R. Vyavahare,
bioRxiv - Bioengineering 2019
Quote:
DiR (1, 1-dioctadecyl-3, 3, 3, 3-tetramethylindotricarbocyanine iodide) (PromoCell GmbH, Heidelberg, Germany) loaded BSA (Seracare ...
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No products found
because this supplier's products are not listed.
Nicholas C. Bauer, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 3-5×104 cells were seeded into each well of a chambered 8-well 1.5H coverglass (Ibidi 80827) and allowed to adhere overnight prior to further manipulation ...
-
No products found
because this supplier's products are not listed.
Yasuhisa Sakamoto, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and fluorescein-PE [N-(fluorescein-5-thiocarbamoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine] was obtained from AAT Bioquest Inc.
-
No products found
because this supplier's products are not listed.
Francesca Baggio, et al.,
bioRxiv - Microbiology 2020
Quote:
... incubated 15 min at RT with DAPI (Novus Biologicals, 1 μg/μl, diluted 1:10000 in methanol), and washed twice with milli-Q water prior to mounting with FluoreGuard mounting medium (Scytek Laboratories) ...
-
No products found
because this supplier's products are not listed.
Hyeogsun Kwon, et al.,
bioRxiv - Immunology 2021
Quote:
Phagocytosis assays were performed by injecting 69 nl of 2% green fluorescent FluoSpheres (vol/vol) in 1X PBS to naïve female mosquitoes (3-to 5-day old) using a Nanoject II injector (Drummond Scientific). After injection ...
-
No products found
because this supplier's products are not listed.
Aakash Basu, et al.,
bioRxiv - Neuroscience 2022
Quote:
... mice were first anaesthetized with isoflurane in oxygen (3-5% during induction, slowly lowered throughout the surgery to 1-2%) while placed in a stereotaxic apparatus (Stoelting). Eyes were lubricated with ophthalmic ointment ...
-
No products found
because this supplier's products are not listed.
Anderson R Frank, et al.,
bioRxiv - Cancer Biology 2022
Quote:
All lentiviruses were produced by co-transfection of HEK 293T/17 cells with plasmid DNA for the respective lentiviral vector and the packaging components psPAX2 and pMD2.G at a 5:3:2 mass ratio (vector:psPAX2:pMD2.G) using TransIT-LT1 (Mirus MIR 2300). Lentiviral supernatants were collected at 48 and 72 hours post-transfection ...
-
No products found
because this supplier's products are not listed.
Fabian Ruperti, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... at 6.0 m/s (3 x 30 s, 5 min pause time) using 1.0 mm zirconia/glass beads (Biospec Products, OK, USA). After centrifugation for 10 min at 15,000 x g and 4 °C with a 5415R benchtop microcentrifuge (Eppendorf ...
-
No products found
because this supplier's products are not listed.
Eleonora Grisard, et al.,
bioRxiv - Cell Biology 2021
Quote:
... rabbit anti-human 14-3-3 1/1000 (EPR6380, GeneTex), rabbit anti-human Lamp1 1/1000 (clone EPR4204 ...
-
No products found
because this supplier's products are not listed.
Eline D. Postma, et al.,
bioRxiv - Genetics 2021
Quote:
... Biomass was washed with cooled 100 % methanol on a PES membrane with a pore size of 0.45 µm (Pall Corporation) which was pre-cooled and wetted with 100 % methanol at -40°C ...
-
No products found
because this supplier's products are not listed.
Hideki Nakamura, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Arp2/3 complex (Cytoskeleton, Inc. RP01P), and GST-WASP-VCA (Cytoskeleton ...
-
No products found
because this supplier's products are not listed.
Evan M. Hess, et al.,
bioRxiv - Neuroscience 2022
Quote:
... supplemented with 5% fetal bovine serum/5% horse serum (Atlanta Biologicals), Glutamax (Gibco) ...
-
No products found
because this supplier's products are not listed.
Matthew D. Kerr, et al.,
bioRxiv - Bioengineering 2022
Quote:
... (4-(1,2,4,5-tetrzain-3-yl)phenyl)methanamine (tetrazine amine) was purchased from Kerafast (FCC659, lot: 2014). 1-bicyclo[2.2.1]hept-5-en-2-ylmethanamine (norbornene amine ...
-
No products found
because this supplier's products are not listed.
Emilia Neuwirt, et al.,
bioRxiv - Immunology 2022
Quote:
... 3 - 5 μM MCC950 (Adipogen), 50 - 200 nM MG132 (Sigma) ...
-
No products found
because this supplier's products are not listed.
Xueao Zheng, et al.,
bioRxiv - Plant Biology 2023
Quote:
... was grinded in liquid nitrogen and were extracted by using 3 mL Methyl-tert-butyl ether/methanol/water (10:3:2.5, V/V) containing 10 ng D4-SA (CDN Isotopes, Pointe-Claire, Canada) for 1 h at room temperature ...
-
No products found
because this supplier's products are not listed.
Andrew J. Lutkewitte, et al.,
bioRxiv - Physiology 2020
Quote:
... or AAV8-GFP-U6-Mogat1-shRNA (sequences 5-UUUCACCCUCAUGGAAUAUUCGUGCCU-3 and 5-CAAGACGCAAUGUAUGAUUCAAUGGGA-3 [20]; pooled (2.0 x 1011 GC total) before injection (Vector Biolabs). For hepatic Mogat1 overexpression ...
-
No products found
because this supplier's products are not listed.
Watcharachai Meemetta, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Forward primer LCHV-MEP93-qF: 5’-GTACTTCATCGCCTACGGAGC-3’ and reverse primer LCHV-MEP93-qR: 5’-TACGTGTGCTTGAGGAGGTC-3’ were synthesized from Bio Basic, Canada ...
-
No products found
because this supplier's products are not listed.
Chiara Battistini, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... sh-C (5’-TAATACGACTCACTATAGGG-3’; HSH096566-LVRU6GP-c); sh-E (5’-TAATACGACTCACTATAGGG-3’; HSH096566-LVRU6GP-e) or with the scrambled control (CSHCTR001-LVRU6GP, Genecopoeia), and the following packaging vectors ...
-
No products found
because this supplier's products are not listed.
Elisa T. Granato, Kevin R. Foster,
bioRxiv - Microbiology 2020
Quote:
... and placed onto a 5 cm diameter glass bottom Petri dish with a 3-cm diameter uncoated n°1.5 glass window (MatTek Corporation), which was then inverted to allow immediate imaging of the spots through air ...
-
No products found
because this supplier's products are not listed.
S. Liu, et al.,
bioRxiv - Cell Biology 2023
Quote:
... containing 0.1% Triton X-100 and blocked 1 hour in 5% goat serum (Jackson Immuno Research, 005-000-121) 3% BSA (Pan Biotech, P06-1391100). Rabbit anti-phospho-ubiquitin ...
-
No products found
because this supplier's products are not listed.
Trung The Tran, et al.,
bioRxiv - Immunology 2022
Quote:
... Cells were fixed and permeabilized with methanol and 0.6% H2O2 and incubated with rabbit monoclonal IgG against SARS-CoV2 NP (Sino Biological). Cells were further incubated with biotinylated goat anti-rabbit IgG (H+L ...
-
No products found
because this supplier's products are not listed.
Chatterjee Amit, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Syntaxin 3 (Synaptic system 110032). Each antibody was 1000-fold diluted in either 5% (w/v ...