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5 Cyanopyridin 3 yl methanol
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Alfa Chemistry Sponsored
Cat# ACM135124719, Inquire Ask
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Millipore Sigma
No products found because this supplier's products are not listed.bioRxiv - Evolutionary Biology 2021Quote: ... 0.1% w/v Ferrozine (Disodium-4-[3-pyridin-2-yl-6-(4-sulfonatophenyl)-1,2,4-triazin-5-yl]benzosulfonate (Sigma-Aldrich) in dd ... -
Promega
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2022Quote: SJ-GBM2 and SF8628 cells were used to determine if reducing WDR82 through inducible knockdown affected cell viability 1×104 cells/100μl were plated in 96-well plates with complete cell culture medium with or without Dox (2μg/ml), and subjected to 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS, Promega) assay. -
Tocris
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... (S)-1-(2-Amino-2-carboxyethyl)-3-(2-carboxy-5-phenylthiophene-3-yl-methyl)-5-methylpyrimidine-2,4-dione (ACET; 1μM; Tocris Bioscience, catalog #2728). -
Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2022Quote: ... 10 μL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, concentration 5 mg/mL, Invitrogen, cat. M6494) in PBS was added to each well containing culture medium and incubated for 2.5 h at 37 °C ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2021Quote: ... in paraffin-embedded sections (8µm) and color was detected with 5-bromo-4-chloroindol-3-yl phosphate/nitrateblue tetrazolium (BCIP/NBT) (Roche). -
Merck
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2021Quote: ... excess media was removed from wells and mf were incubated with 0.5 mg/ml MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (Merck) in PBS at 37°C for 90 min ... -
VWR
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2021Quote: ... and washed/fixed with 3:1 methanol (VWR)/glacial-acetic acid (Carl Roth ... -
Avanti Polar Lipids
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2022Quote: ... and the soluble dye 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate Sulforhodamine B) were purchased from Avanti Polar Lipids (IL, USA). The fluorescent lipid dye ATTO 647N-DOPE was from ATTO-TEC GmbH (Siegen ... -
abcam
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2021Quote: ... and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) reagent (5 mg/mL MTT (Abcam ab146345) in PBS ... -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2021Quote: ... E2D2/3 (5’-AACAGUAAUGGCAGCAUUUGU-3’) and E2D4 siRNAs (5’ – CCGAAUGACAGUCCUUACCAA-3’) all from Qiagen, USA(83). -
New England Biolabs
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2022Quote: ... Cells were preserved in a 1:3 glacial acetic acid: methanol (Biolabs-chemicals) solution and karyotyped using g-banding. -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... 341F (5’-CCTACGGGNGGCWGCAG-3’) and 805R (5’-GACTACHVGGGTATCTAATCC-3’) or (2) 341F (5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3’) and 806R (5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGGACTACHVGGGTWTCTAAT-3’) (TaKaRa Bio, Shiga, Japan). The second PCR was performed to add the index sequences for Illumina sequencing with barcode sequences ... -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Immunology 2017, published in European Journal of Immunology doi: 10.1002/eji.201847498Quote: ... gRNAs composed of 5’-CACCGAAGACATACACCACCACGG-3’ annealed with 5’-AAACCCGTGGTGGTGTATGTCTTC −3’ and 5’-CACCGCATCAGTCCTCCAGCCAG −3’ annealed with 5’-AAACCTGGCTGGAGGACTGATGC −3’ were cloned into the pX330 vector (Addgene), amplified by PCR and transcribed using the Megashortscript T7 transcription kit (Life Technologies) ... -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2020Quote: ... 5% methanol) using a trans-blot system (Bio-Rad). Blocking the PVDF membrane was performed by shaking in TBS-T (10 mM Tris-HCl ... -
Alfa Aesar
No products found because this supplier's products are not listed.Cited in Tumor Suppressor p53 Restrains Cancer Cell Dissemination by Modulating Mitochondrial DynamicsbioRxiv - Molecular Biology 2021Quote: ... MTT (3-(4,5-dimethylthiazol-2-yl-2, 5-diphenyl tetrazolium bromide)) was purchased from Alfa Aesar (Thermo Fisher Scientific ... -
Eurogentec
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2022Quote: ... engMutfwd 5’-AGAACAGACGAATCTACAGCCGAA-3’ and engintron2rev 5’-AGCATGTTTTAACAAGACGGCAG-3’ primers and HotGoldStar PCR mix (Eurogentec). -
Calbiochem
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2020, published in Biochimica et Biophysica Acta (BBA) - General Subjects doi: 10.1016/j.bbagen.2021.129846Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent was obtained from Calbiochem (San Diego, CA, USA). -
Agilent
No products found because this supplier's products are not listed.Cited in Spermatoproteasome-deficient mice are proficient in meiotic DNA repair but defective in meiotic exitbioRxiv - Cell Biology 2018Quote: ... with primers flanking exons 1 and intron 1 (Primer F 5-CTTCTCGGTATGACAGGGCAATC-3’ and R 5’-ACTCTACCTCCACTGCCAACCTG-3’) and either direct sequenced or subcloned into pBlueScript (Stratagene) followed by Sanger sequencing ... -
GE Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Animal Behavior and Cognition 2020Quote: ... CG42797EcoRI_F: 5’-CCGgaattcATGGAGCCACCAGCT-3’ and CG42797XhoI_R: 5’-TAGAGGTACCctcgagCTACTCAATGCCGAACGTGTTG-3’ and cloned by enzymatic digestion into a pGEX6P1(GE Healthcare). -
Illumina
No products found because this supplier's products are not listed.Cited in Dual roles of mTORC1-dependent activation of the ubiquitin-proteasome system in muscle proteostasisbioRxiv - Physiology 2021Quote: ... 3’ and 5’ adaptors (Illumina) were ligated and the resulting product was reverse transcribed to generate cDNA by PCR ... -
Electron Microscopy Sciences
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... Fibroblasts were fixed with 3% (v/v) formaldehyde in PBS (methanol-free, Electron Microscopy Science, Hatfield, PA, USA) for 20 min at room temperature ... -
Santa Cruz
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2017, published in Proceedings of the National Academy of Sciences doi: 10.1073/pnas.1717594115Quote: ... We labeled both mutant Arp2/3 complexes overnight at 4°C with 10x molar excess 4,5-Bis(1,3,2-dithiarsolan-2-yl)-3,6-dihydroxyspiro[isobenzofuran-1(3H),9-[9H]xanthen]-3-one (FlAsH-EDT2) (Santa Cruz Biotechnology). Protein complexes were buffer exchanged into low salt buffer using a 30K Amicon Ultra centrifugal filter unit ... -
PerkinElmer
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019Quote: ... cyanine 3 and cyanine 5 (Perkin Elmer). The ACD 3-plex negative control probe was run in parallel on separate sections in each experiment to assess the background level and set the acquisition parameter ... -
Jena Bioscience
No products found because this supplier's products are not listed.Cited in Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogensbioRxiv - Biochemistry 2021Quote: ... 3’-deoxyadenosine-5’-triphosphate (3’-dATP, Jena Bioscience) at 2 mM and MgCl2 at 4 mM was incubated for 15 minutes at room temperature ... -
Peprotech
No products found because this supplier's products are not listed.Cited in In Vitro Assay for the Detection of Network Connectivity in Embryonic Stem Cell-Derived CulturesbioRxiv - Bioengineering 2018Quote: ... and 5 ng/mL neurotrophin-3 (NT-3; Peprotech #450-03). After 24 hours ... -
GenScript
No products found because this supplier's products are not listed.bioRxiv - Genetics 2021Quote: ... sequences flanked by overhangs for Gibson Assembly matching the vector insertion site (5’-CCGCATGCTTAATTAAGAAGGAGATATACAT-3’) – array sequence – (5’-GACTACAAGGATGACGACGACAAG-3’) were synthesized (Genscript) and obtained in a pUC57-Kan vector ... -
Polysciences
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2019, published in Cell Death & Disease doi: 10.1038/s41419-020-2342-2Quote: ... fixed in 4% methanol-free formaldehyde (Polysciences) in phosphate buffer ... -
Vector Labs
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... Endogenous peroxidase activity and non-specific binding were blocked by incubation with 3% hydrogen peroxide in methanol for 15 minutes followed by 5% normal goat serum (Vector Laboratories, CA, USA) with 0.1% TWEEN-20 in PBS for 1 hour ... -
Cayman Chemical
No products found because this supplier's products are not listed.Cited in Vitamin D regulation of a SOD1-to-SOD2 antioxidative switch to prevent osteosarcoma transformationbioRxiv - Cancer Biology 2020Quote: ... The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay was performed according to the manufacturer’s recommendation (10009365, Cayman Chemicals). After incubation with the MTT reagent ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: [2] GAL4d from pBPGAL4.1Uw (Addgene plasmid #26226)(Primers:ADdomain,forward,5’-caggcggccgccataTGCATGGATCCGCCAACTTC AACCAGAGTGG-3’, reverse, 5’-tatcgatagacgtca CTACTCCTTCTTTGGGTTCGG-3’; BD domain ... -
IDT DNA
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... and host Actb (Forward: 5’-AGCTCATTGTAGAAGGTGTGG-3’; Reverse: 5’ - GGTGGGAATGGGTCAGAAG-3’; Probe: 5’-TTCAGGGTCAGGATACCTCTCTTGCT-3’; IDT DNA) against plasmid standard curves using TAQman universal master mix II on a StepOnePlus real time PCR system (Thermo Fisher Scientific). -
Origene Technologies
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... ShRNA sequences against rat Zdhhc3 (5’-GAGACATTGAACGGAAACCAGAATACCTC-3’) and Zdhhc7 (5’-ATGACATGGCTTCTGGTCGTCTATGCAGA-3’) were purchased from Origene and subcloned ... -
R&D Systems
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2021Quote: ... 5 ng/mL recombinant murine IL-3 (R&D Systems, and 5% stem cell factor-containing supernatant from CHO-KL cells for 4-6 weeks until maturation ... -
Chembridge
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in Journal of Cell Biology doi: 10.1083/jcb.201911154Quote: ... Kinesore (3,5-dibromo-N′-[2,5-dimethyl-1-(3-nitrophenyl)-1H-pyrrol-3-yl]methylene}-4-hydroxybenzohydrazide) was obtained from Chembridge Corporation (Cat ... -
Toronto Research Chemical
No products found because this supplier's products are not listed.Cited in Anti-inflammatory role of GM1 and modulatory effects of gangliosides on microglia functionsbioRxiv - Neuroscience 2020Quote: ... and N-[(1R,2R)-1-(2,3-Dihydrobenzo[b][1,4]dioxin-6-yl)-1-hydroxy-3- (pyrrolidin-1-yl)propan 2-yl] nonanamide (GENZ-123346) was obtained from Toronto Research Chemicals (TRC G363450) and solubilized in DMSO ... -
MedChemExpress
Cat# HY-100839-25 mg, 25 mg, USD $660.0 AskbioRxiv - Microbiology 2021Quote: 1-(2,3-di(Thiophen-2-yl)quinoxalin-6-yl)-3-(2-methoxyethyl)urea (CAS 508186-14-9 – MCE-MedChemExpress) is an Acetyl-CoA Synthetase 2-specific and potent inhibitor ... -
Leica
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... 2.3% methanol and 1–3% H2O in Acetone) tempered at −90°C in FS device (Leica AFS2) equipped with a robotic solution handler (Leica FSP) ... -
Eppendorf
No products found because this supplier's products are not listed.bioRxiv - Immunology 2018Quote: ... and 0.4 U 5′-3′ RNase Inhibitor (Eppendorf)) and immediately frozen on dry ice ... -
Lucigen
No products found because this supplier's products are not listed.Cited in Expression of the miR-302/367 microRNA cluster is regulated by a conserved long non-coding host genebioRxiv - Genetics 2018Quote: Rapid Amplification of cDNA Ends (RACE) for the 5’ and 3’ ends was performed using ExactSTART™ Eukaryotic mRNA 5′- & 3′-RACE Kit (Epicentre). For 5’-RACE “mmiR-302 t Rev4” was used as reverse and internal primer ... -
Lonza
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2021Quote: ... The WT and mutant genes were then amplified from these expression plasmids using the PCR primers 5’-GCGCTAGCAGCACCCATATGCCTGAG-3’and 5’-ACGAAGCTTTCAGTGGTGGTGGTGGT-3’and subcloned into the pMaxCloning vector (Lonza, VDC-1040) via NheI and BamHI sites to generate the pMax_WT_NEIL1 and pMax_Mutant_NEIL1 expression plasmids that were utilized throughout this analysis. -
Corning
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2019Quote: ... of yeast culture was incubated with 5 µL of Nile red solution (0.1 mg/mL in methanol) into 96-well solid black plates (Corning Inc.). The fluorescence was measured before and after the addition of the Nile red solution with excitation and emission at 485 and 538 nm ... -
Sutter Instruments
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2022Quote: ... Recording electrodes (3-5 MΩ, Borosilicate Glass, Sutter Instrument) were prepared by a micropipette puller (Sutter Instrument ... -
Cambridge Isotope Labs
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2017, published in Nature Communications doi: 10.1038/s41467-018-03562-9Quote: ... Sample temperatures were calibrated using the 4% methanol + 96% methanol-d4 sample (DLM-5007, Cambridge Isotope Laboratories). -
Phenomenex
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2018, published in Journal of Proteome Research doi: 10.1021/acs.jproteome.8b00166Quote: ... with 3 cm of 5 μm Aqua C18 resin (Phenomenex, 00G-4299-E0) followed by 3 cm of Partisphere strong cation exchange (SCX ... -
The Jackson Laboratory
No products found because this supplier's products are not listed.Cited in Argininosuccinic aciduria fosters neuronal nitrosative stress reversed by Asl gene transferbioRxiv - Genetics 2018, published in Nature Communications doi: 10.1038/s41467-018-05972-1Quote: ... 5’-GGTTCTTGGTGCTCATGGAT-3’ (sense), 5’-GCCAGAGGCCACTTGTGTAG-3’ (WT, antisense) and 5’-CATGACAGCTCCCATGAAGA-3’ (AslNeo/Neo mice, antisense) provided by Jackson Laboratory (Bar Harbor, ME). Amplification conditions were 95°C for 10 minutes then 40 cycles of 94°C for 30 seconds ... -
World Precision Instruments
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2018, published in The Journal of Neuroscience doi: 10.1523/jneurosci.0168-19.2019Quote: ... 3-5 MΩ borosilicate glass pipettes (WPI) were filled with internal solution containing ... -
MP Biomedicals
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2021Quote: Metabolites were extracted from cell pellets using 3 mL 2:1 methanol-water solvent and 0.5 ml of lysing beads (Lysing Matrix D from MP Biomedicals, LLC). The mixture was vortexed and freeze-thawed to extract the metabolites ... -
Jackson ImmunoResearch
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2021Quote: ... Cy-3 or Cy-5 (1:500, Jackson ImmunoResearch). Sections were counterstained with a fluorescent Nissl stain (NeuroTrace 435/445 blue ... -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2022Quote: ... and 5 μg/mL of CD40 antibody ligand (clone: HM40-3; BioLegend). -
Cell Signaling Technology
No products found because this supplier's products are not listed.Cited in Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: ... permeabilized with methanol and stained for H3K27me3 (#C36B11, Cell Signaling Technology), PICH (inhouse antibody ...