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5α CHOLESTAN 3 ONE
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MedChemExpress Sponsored
Cat# HY-107826-10 mM * 1 mL, 10 mM * 1 mL, USD $55.0 Ask
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Millipore Sigma
No products found because this supplier's products are not listed.bioRxiv - Genetics 2021Quote: ... then were stimulated with either 10 nM DHT (5α-Androstan-17β-ol-3-one, Dihydrotestosterone, A8380, Sigma) or EtOH (Vehicle ... -
New England Biolabs
No products found because this supplier's products are not listed.Cited in Unblocking genome editing of major animal mycoplasmas using CRISPR/Cas9 base editor systemsbioRxiv - Microbiology 2022Quote: ... Escherichia coli NEB-5α (NEB, C2987H) was used for plasmid propagation and was cultivated in LB Broth (ThermoFisher ... -
Steraloids
No products found because this supplier's products are not listed.Cited in CYP154C5 Regioselectivity in Steroid Hydroxylation Explored by Substrate and Protein EngineeringbioRxiv - Biochemistry 2020Quote: ... 3-deoxydehydroepiandrostendione (10) and 5α-androstan-3-one (11) were purchased from Steraloids Inc ... -
Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2022Quote: ... One hundred nanomolar YOYO-3 Iodide (ThermoFisher Scientific) was added to label dead cells ... -
Zymo Research
No products found because this supplier's products are not listed.Cited in Programmed genome editing of the omega-1 ribonuclease 1 of the blood fluke, Schistosoma mansonibioRxiv - Molecular Biology 2018, published in eLife doi: 10.7554/elife.41337Quote: Escherichia coli Zymo 5α (Zymo Research) cells were transformed with lentiviral plasmid pLV-ω1X1 and cultured in LB broth in 100 μg/ml ampicillin at 37°C ... -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2018Quote: ... with one Tungsten Carbide Beads (3 mm, Qiagen) and lysis buffer (5X of the tissue weight ... -
Promega
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2020Quote: ... Caspase 3/7 activation was measured using the Apo-ONE Homogenous Caspase-3/7 Assay (Promega), following the manufacturers protocol ... -
Greiner
No products found because this supplier's products are not listed.Cited in Tau assemblies enter the cytosol in a cholesterol sensitive process essential to seeded aggregationbioRxiv - Cell Biology 2021Quote: ... 3×104 cells were seeded per well into a white 96-well (Greiner bio-one, 655098) poly-L-lysine (RnD Systems ... -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Genetics 2018, published in Nucleic Acids Research doi: 10.1093/nar/gky748Quote: ... Quantity One (BioRad) software was used to analyze gel images ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... Reverse: 3’-GGTCCACCAAACGTAATGCG-5’) were performed using KAPA SYBR FAST ONE-STEP qRT-PCR kits (Roche) according to manufacturer’s instructions on a Lightcycler 480 Instrument-II (Roche). -
Agilent
No products found because this supplier's products are not listed.bioRxiv - Evolutionary Biology 2018, published in eLife doi: 10.7554/elife.35122Quote: cRNA targets labeled with cyanine-3 were synthesized from 400 ng total Hydra RNA using a Quick Amp Labeling Kit for one color detection (Agilent Technologies). A set of fluorescently labeled cRNA targets was employed in a hybridization reaction with 4 × 44K Custom-Made Hydra viridissima Microarray (Agilent Technologies ... -
abcam
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... 4-(3-(cyclopentyloxy)-4-methoxyphenyl)pyrrolidin-2-one (rolipram; Abcam); 4-[(2S)-2-[(5-isoquinolinylsulfonyl)methylamino]-3-oxo-3-(4-phenyl-1-piperazinyl)propyl] phenyl isoquinolinesulfonic acid ester (KN-62 ... -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020, published in Cell doi: 10.1016/j.cell.2020.09.033Quote: ... targeting ACE2 (5’-TGGATACATTTGGGCAAGTG −3’) and one targeting B4GALT7 (5’-TGACCTGCTCCCTCTCAACG-3’) was cloned into the lentiGuide-Puro plasmid (Addgene plasmid #52963) following published procedure (Sanjana et al. ... -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2021Quote: ... was incubated with antibodies at 4°C for one hour: 3 µl (0.5 µg/µl) c-Myc monoclonal antibody (Cat. No. 631206, TaKaRa), or 1 µl (1.1 µg/µl ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2021Quote: ... 2.4 mm OD) attached on one end to a 3 ml syringe (BD 309657) and on the other positioned at one end of the test tank ... -
Corning
No products found because this supplier's products are not listed.Cited in TRIPLE-NEGATIVE BREAST CANCER CELLS RECRUIT NEUTROPHILS BY SECRETING TGF-β AND CXCR2 LIGANDSbioRxiv - Immunology 2021Quote: ... transwell migration chambers with membrane inserts of 3 μm diameter pore size (Corning and Greiner Bio-One) were used ... -
Santa Cruz
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2017, published in Proceedings of the National Academy of Sciences doi: 10.1073/pnas.1717594115Quote: ... We labeled both mutant Arp2/3 complexes overnight at 4°C with 10x molar excess 4,5-Bis(1,3,2-dithiarsolan-2-yl)-3,6-dihydroxyspiro[isobenzofuran-1(3H),9-[9H]xanthen]-3-one (FlAsH-EDT2) (Santa Cruz Biotechnology). Protein complexes were buffer exchanged into low salt buffer using a 30K Amicon Ultra centrifugal filter unit ... -
Tocris
No products found because this supplier's products are not listed.bioRxiv - Physiology 2021Quote: ... L-364,373 (R-L3 or 5-(2-Fluorophenyl)-1,3-dihydro-3-(1H-indol-3-ylmethyl)-1-methyl-2H-1,4-benzodiazepin-2-one) (Tocris Cookson; Bristol, UK) and mefenamic (dimethylphenylaminobenzoic ... -
Merck
No products found because this supplier's products are not listed.bioRxiv - Synthetic Biology 2021Quote: ... Eluted protein samples were combined in one tube and desalinated using an ultrafiltration tube (Amicon Ultra 3 kDa molecular weight cut-off, Merck/Millipore) with Buffer C (50 mM Tris-HCl (pH 7.5) ... -
Peprotech
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2018, published in PLOS ONE doi: 10.1371/journal.pone.0203244Quote: ... and IL-6) and one murine (Flt-3 ligand) recombinant proteins (all obtained from Peprotech). Isolated PBMNCs were cultured for five days at a cell density of 2.0×106 /2 mL per well in QQ culture medium (Stem Line II ... -
Illumina
No products found because this supplier's products are not listed.bioRxiv - Evolutionary Biology 2021Quote: ... All three reactions were pooled in equal-molar ratios and sequenced (paired end 300 bp) on one lane of Illumina MiSeq version 3 chemistry (Illumina, San Diego, California). The final reaction comprising 21 libraries with DNA concentrations 11.4 – 20.0 ng/μl was performed and sequenced as above. -
Cell Signaling Technology
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2021Quote: ... One microgram of H3K4me3 (Cell Signaling Technology, #9751) or H3K27ac (Active Motif ... -
VWR
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2018, published in Antimicrobial Agents and Chemotherapy doi: 10.1128/aac.02410-18Quote: ... while 2-phenyl-1,2-benzisoselenazol-3(2H)-one (ebselen, Eb, PubChem SID 856002) and glucosamine hydrochloride were obtained from VWR International (West Chester ... -
Leica
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2021Quote: ... Mice were anesthetized with a mixture of oxygen (1L/min) and isoflurane 3% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was shaved ... -
Electron Microscopy Sciences
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2018, published in Nature Communications doi: 10.1038/s41467-020-14752-9Quote: ... One milliliter of the cells was fixed in phosphate-buffered saline containing 3% glutaraldehyde/3% paraformaldehyde (Electron Microscopy Sciences) at room temperature for 15 min ... -
3M
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2021Quote: ... One SCX StageTip was prepared per sample using 3 plugs of SCX material (3M) topped with 2 plugs of C18 material ... -
BioLegend
No products found because this supplier's products are not listed.Cited in Expression of Tim-3 drives naïve Treg to an effector-like state with enhanced suppressive activitybioRxiv - Immunology 2020Quote: ... Tim-3 clone RMT 3-23 (BioLegend) and clone FAB1529 (R&D) ... -
Vector Labs
No products found because this supplier's products are not listed.Cited in Sequential regulation of maternal mRNAs through a conserved cis-acting element in their 3’UTRsbioRxiv - Developmental Biology 2018, published in Cell Reports doi: 10.1016/j.celrep.2018.12.007Quote: ... one-drop of Vectashield (Vector Labs, Inc.) was added and then the tissue was mounted on a glass slide and a coverslip was placed on the slide ... -
Beckman
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2020Quote: ... One was through twice daily measurement of 2 or 3 levels of QC specimens obtained from Beckman Coulter Inc ... -
R&D Systems
No products found because this supplier's products are not listed.Cited in Genetic Variant in 3’ Untranslated Region of the Mouse Pycard Gene Regulates Inflammasome ActivitybioRxiv - Genetics 2021Quote: ... 3 ng/ml mouse IL-3 (R&D Systems) and 20% L-cell conditioned medium ... -
GE Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2017, published in NeuroImage: Clinical doi: 10.1016/j.nicl.2017.06.013Quote: ... one with a field strength of 1.5 T and one of 3.0 T (MR450 and MR750, GE Healthcare, Milwaukee, WI, USA). The assignment of the cases to either of these scanners was not controlled in the current study ... -
Avanti Polar Lipids
No products found because this supplier's products are not listed.Cited in The lipid droplet protein Pgc1 controls the subcellular distribution of phosphatidylglycerolbioRxiv - Cell Biology 2018, published in FEMS Yeast Research doi: 10.1093/femsyr/foz045Quote: ... 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and 1-palmitoyl-2-oleoyl-sn-3-phosphoethanolamine (3:1; Avanti Polar Lipids) were dissolved in chloroform ... -
PerkinElmer
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2021Quote: ... cyanine 3 (PerkinElmer #NEL744E001KT), and cyanine 5 (PerkinElmer #NEL745E001KT) ... -
Alfa Aesar
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... AZVIII-40A (1,2-Benzisothiazol-3(2H)-one) was purchased from Alfa Aesar. Other protease inhibitors in table below were purchased from TargetMol. -
Invivogen
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2021Quote: ... one from Invivogen of high molecular weight (HMW ... -
Calbiochem
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2020Quote: ... The 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one (PD98059, MEK inhibitor) and STAT3 inhibitor VI were from Calbiochem (Darmstadt, Germany). The EGF was from Millipore (Billerica ... -
Jackson ImmunoResearch
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2017, published in Biology of the Cell doi: 10.1111/boc.201800007Quote: ... washed three times in PBS and incubated for one hour with Alexa 488- or cyanine 3-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA). After washing ... -
The Jackson Laboratory
No products found because this supplier's products are not listed.bioRxiv - Physiology 2018, published in Frontiers in Physiology doi: 10.3389/fphys.2019.00016Quote: ... One parent (Jackson Labs stock #006410) had homozygous expression of a ChAT promoter to direct the expression of Cre recombinase (Rossi et al. ... -
Cayman Chemical
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018Quote: ... one of which had LY294002 (Cayman Chemical) added to 100 µM (to prevent PkbA recruitment to macropinosomes and activation ... -
Lonza
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2020Quote: ... One million cells were electroporated (Nucleofector, Lonza), and after 48h ... -
Bioline
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... The MyTaq One-Step RT-PCR Kit (Bioline) was used to generate and amplify cDNA from viral RNA isolated from nasal wash samples (as above) ... -
Stemcell Technologies
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2022Quote: ... and Neurotrophin-3 (NT-3; 20 ng/ml, Stemcell technologies) and the culture medium was changed every 3-4 days ... -
World Precision Instruments
No products found because this supplier's products are not listed.Cited in Oxytocin via oxytocin receptor excites neurons in the endopiriform nucleus of juvenile micebioRxiv - Neuroscience 2022Quote: ... glass pipettes (3-8 MΩ, 1B150F-3, World Precision Instruments) were pulled using a horizontal puller (Sutter Instruments ... -
GeneCopoeia
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... using All-in-One qPCR Mix (GeneCopoeia). Quantitative PCR consisted of 40 cycles ... -
Worthington Biochemical
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004183, 5 gm, $825.0 AskbioRxiv - Neuroscience 2020Quote: ... incubated with 3% collagenase type 3 (Worthington), 3 U/ml dispase (Worthington ... -
Eppendorf
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... and a volume corresponding to an OD600nm of one (109 cells) was pelleted at 14,000 x g for one minute (Eppendorf MiniSpin). 20 µl of supernatant was mixed with 50 µl BioLux Gaussia Luciferase Assay substrate (New England Biolabs ... -
Charles River Labs
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2018, published in Journal of Neural Engineering doi: 10.1088/1741-2552/aae90eQuote: ... one male Long Evans rat (Charles River Laboratories) was implanted with a micro-drive array with independently adjustable tetrodes (implant coordinates −3.66 mm AP and 2.4 mm ML relative to bregma) ... -
Molecular Devices
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2021Quote: ... One was a GeneClamp 500 (Molecular Devices, Sunnyvale, CA) interfaced to a computer via a Digidata 1200 device (Molecular Devices ... -
Biotek
No products found because this supplier's products are not listed.Cited in Toxoplasma gondii secreted effectors co-opt host repressor complexes to inhibit necroptosisbioRxiv - Microbiology 2020Quote: ... on Cytation 3 (BioTek) multimode plate imager according to the manufacturer’s protocol ... -
Polysciences
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2019, published in NeuroToxicology doi: 10.1016/j.neuro.2019.10.001Quote: ... One hundred μL of 0.1% chitosan solution (Polysciences, Valley Road, Warrington) prepared in 1% acetic acid (pH 5 ...