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No products found
because this supplier's products are not listed.
Manuele Rebsamen, et al.,
bioRxiv - Cell Biology 2022
Quote:
Antibodies used were SLC7A5 (Cell Signaling, #5347), SLC3A2 (Santa Cruz ...
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No products found
because this supplier's products are not listed.
Renate Hausinger, et al.,
bioRxiv - Cell Biology 2020
Quote:
Staining of cell surface antigens was performed with antibodies from Invitrogen-ThermoFisher except for PE-Cy5.5-streptavidin conjugate ...
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No products found
because this supplier's products are not listed.
Thomas N. Burn, et al.,
bioRxiv - Immunology 2022
Quote:
... and antibodies against respective surface antigens (BD Biosciences ...
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No products found
because this supplier's products are not listed.
King Faisal Yambire, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Ferritin light chain (Abcam), TOM20 (Proteintech) ...
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No products found
because this supplier's products are not listed.
Qing Tang, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... The following antibodies specific for the cell surface antigens from Biolegend were used for the flow cytometry ...
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No products found
because this supplier's products are not listed.
Yasmin Dar, et al.,
bioRxiv - Microbiology 2021
Quote:
... HRP-conjugated α-Light Chain-specific secondary antibodies (Jackson ImmunoResearch) were used to avoid detecting the primary antibodies’ heavy chains.
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No products found
because this supplier's products are not listed.
Fanchong Jian, et al.,
bioRxiv - Immunology 2023
Quote:
Antibody heavy and light chain genes were synthesized by GenScript, separately inserted into vector plasmids (pCMV3-CH ...
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No products found
because this supplier's products are not listed.
Kylie M. Konrath, et al.,
bioRxiv - Immunology 2021
Quote:
... Goat anti-Human Lambda Light Chain Antibody HRP Conjugated (Bethyl Laboratories) at 1:10000 ...
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No products found
because this supplier's products are not listed.
Michael Korenkov, et al.,
bioRxiv - Immunology 2023
Quote:
... Monoclonal antibodies were produced in 50 ml of 0.8×106 HEK293-6E cells by co-transfection of heavy chain (IgG1) and light chain (Igλ, Igκ) antibody expression plasmids with polyethylenimine (PEI; Sigma-Aldrich). Cells were maintained at 37°C and 5% CO2 in FreeStyle 293 Expression Medium (Thermo-Fisher ...
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No products found
because this supplier's products are not listed.
Quynh T. Phan, et al.,
bioRxiv - Microbiology 2021
Quote:
... anti-light chain antibody (Santa Cruz Biotechnology ...
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No products found
because this supplier's products are not listed.
Ioanna Tzani, et al.,
bioRxiv - Cell Biology 2021
Quote:
... the light chain by the anti-human kappa light chain antibody (1:2,500; Biorad, cat.no. STAR 127) and GAPDH by the anti-GAPDH monoclonal antibody (1:10,000 ...
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No products found
because this supplier's products are not listed.
Stephen Graham, et al.,
bioRxiv - Immunology 2020
Quote:
Heavy and light chain vectors were verified by sequencing and transformed into DH5alpha cells (NEB). The transformed cells were grown and the plasmid was purified by midiprep (Macherey-Nagel) ...
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No products found
because this supplier's products are not listed.
Yushan Li, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... Guinea Pig anti-Rabbit IgG (Heavy & Light Chain) secondary antibody (ABIN101961; antibodies-online) was diluted in 100 µl of Dig-wash buffer and cells were incubated for 1 hour ...
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No products found
because this supplier's products are not listed.
Teha Kim, et al.,
bioRxiv - Immunology 2023
Quote:
... or the mouse kappa light chain plasmid (InVivoGen). The gBlock DNA and plasmids were cut with the specified enzymes ...
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No products found
because this supplier's products are not listed.
Israa Al Jamal, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and dual-color reagent Anti-human Kappa light chain/Anti-Human Lambda light chains/RPE (Dako, FR481 X0935). Results were analyzed with Kaluza software version 2.1 (Beckman coulter).
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No products found
because this supplier's products are not listed.
Carlos J Nogueras-Ortiz, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Resuspended total EVs were incubated with 4 μg of anti-GLAST (i.e. antibody against the astrocyte cell surface antigen-1; RRID: AB_2733473; Miltenyi Biotec, Auburn, CA), anti-human CD171 (i.e ...
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No products found
because this supplier's products are not listed.
Jakub Netolicky, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Surface antigens were labeled with primary antibody (rabbit anti-GFP, 1:1000, Merck) for 15 min on ice ...
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No products found
because this supplier's products are not listed.
Meghan Robinson, et al.,
bioRxiv - Bioengineering 2021
Quote:
... 36 million cells were used for sorting with the Sertoli cell marker Thy-1 Cell Surface Antigen (THY1/CD90) using a PE-conjugated antibody for THY1/CD90 (R&D Systems, FAB2067P) and the EasySep™ Human PE Positive Selection Kit II (STEMCELL Technologies ...
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No products found
because this supplier's products are not listed.
Kem A. Sochacki, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Cells were transfected with mCherry – clathrin light chain B (Addgene Plasmid #55019) and EGFP – clathrin light chain A52 plasmids ...
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No products found
because this supplier's products are not listed.
Kamalakannan Vijayan, et al.,
bioRxiv - Immunology 2020
Quote:
Plasmid DNA encoding the heavy and light chains of recombinant monoclonal antibodies and polyethylene imine (Polysciences, Warrington, PA) were mixed at a 1:4 ratio ...
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No products found
because this supplier's products are not listed.
Sedat Odabas, et al.,
bioRxiv - Bioengineering 2021
Quote:
... Quantitative Real-Time polymerase chain reaction (qRT-PCR; Light-Cycler 480 instrument Roche Diagnostics, Mannheim, Germany) analysis was performed for Collagen Type I (COL1A1) ...
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No products found
because this supplier's products are not listed.
Wei Liu, et al.,
bioRxiv - Cell Biology 2023
Quote:
... MYLK polyclonal antibody and myosin light chain polyclonal antibody were purchased from Proteintech. ROCK1 or ROCK2 rabbit mAb ...
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No products found
because this supplier's products are not listed.
J. Andrew Duty, et al.,
bioRxiv - Microbiology 2022
Quote:
Kinetic interactions between the antibodies and his-tagged antigen proteins were measured at room temperature using Biacore T200 surface plasmon resonance (GE Healthcare). Anti-human fragment crystallizable region (Fc region ...
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No products found
because this supplier's products are not listed.
Dora Buzas, et al.,
bioRxiv - Biochemistry 2023
Quote:
... and Lambda light chains (Catalog #1060-01, Southern Biotech). After overnight incubation at 4□°C ...
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No products found
because this supplier's products are not listed.
Beiyuan Liang, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... After antigen retrieval using Antigen Unmasking Solution (Vector Laboratories), tissue slides were blocked with 10% Goat Serum (Vector Laboratories ...
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No products found
because this supplier's products are not listed.
Ying Dong, et al.,
bioRxiv - Immunology 2022
Quote:
... anti-mouse lambda light chain (Novus Biologicals, Catalog number NB7552), anti-FLAG (Sigma Aldrich ...
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No products found
because this supplier's products are not listed.
Michael Ferns, et al.,
bioRxiv - Neuroscience 2024
Quote:
... A dye-conjugated light chain-specific anti-rabbit secondary antibody (Jackson Labs) was used to detect bound Kv5.1 antibody ...
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No products found
because this supplier's products are not listed.
Soumya Palliyil, et al.,
bioRxiv - Microbiology 2021
Quote:
... plasmids bearing chimeric antibody heavy and light chain genes were prepared (EndoFree Plasmid Mega prep Kit, QIAGEN) and transfected into Human Embryonic Kidney cells (HEK293F ...
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No products found
because this supplier's products are not listed.
Tamara Boschert, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... HEK cells were transfected with both heavy and light chain BCR constructs using FuGENE HD transfection reagent (Promega) in a 1:3 ratio ...
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No products found
because this supplier's products are not listed.
Lei Jin, et al.,
bioRxiv - Neuroscience 2023
Quote:
... surface vasculature map was draw on a notebook for each mouse under microscope with light source (Leica M50) and put ‘*’ marker near the location of FOVs in this map for reference when doing the first imaging session ...
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No products found
because this supplier's products are not listed.
Matthew D. Kerr, et al.,
bioRxiv - Bioengineering 2022
Quote:
... 50μL of anti-rat κ immunoglobulin light chain antibody (MAR 18.5, Bio X Cell, lot: 752020J2) was administered every other day starting on the second day of depletion ...
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No products found
because this supplier's products are not listed.
Stijn De Munter, et al.,
bioRxiv - Immunology 2023
Quote:
... Surface antigen expression was measured with the CytoFLEX LX (Beckman Coulter) and data was analyzed with the CytExpert Software Version 2.4 (Beckman Coulter ...
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No products found
because this supplier's products are not listed.
Mladen Jergović, et al.,
bioRxiv - Immunology 2022
Quote:
... Cells were stained with surface antibodies in PBS (Lonza) + 2% FCS ...
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No products found
because this supplier's products are not listed.
Jan Christoph Thiele, et al.,
bioRxiv - Biophysics 2023
Quote:
... Scattered light from the coverslip surface is collected using an objective (60×, 1.1NA, water dipping, Olympus) and is imaged onto four different cameras by an achromatic tube lens (f = 300 mm ...
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No products found
because this supplier's products are not listed.
Fangwu Wang, et al.,
bioRxiv - Immunology 2024
Quote:
... cells in a single-cell suspension were stained with fluorochrome-conjugated antibodies specific to surface antigens (listed in Supplemental Table 1) in Hanks’ Balanced Salt Solution (HBSS, STEMCELL Technologies) supplemented with 2% FBS ...
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No products found
because this supplier's products are not listed.
Thadeu Estevam Moreira Maramaldo Costa, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... Antibodies against human PCNA (proliferating cell nuclear antigen, rabbit) were purchased from Sino Biological (China). DNase I recombinant RNase free was purchased from Roche Life Sciences (Germany).
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No products found
because this supplier's products are not listed.
Jianlong Zhai, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... the antigen-antibody complexes were detected using Odyssey XF (LI-COR, USA). The signal intensities of target bands were analyzed using ImageJ software (National Institutes of Health ...
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No products found
because this supplier's products are not listed.
Luis A. Williams, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and cell culture surfaces pre-coated with Matrigel (Corning) according to manufacturers’ recommendations ...
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No products found
because this supplier's products are not listed.
Lizandra Jimenez, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Cells were imaged via light microscopy (Nikon Eclipse TE2000E) at 48 and 72 hr post plating ...
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No products found
because this supplier's products are not listed.
Steven V. John, et al.,
bioRxiv - Cell Biology 2024
Quote:
... gondii surface antigen 1 (SAG1, rabbit, 1:300; Genetex GTX38936) overnight at 4°C and then thoroughly washed with DPBS ...
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No products found
because this supplier's products are not listed.
Paritosh Parashar, et al.,
bioRxiv - Immunology 2022
Quote:
... Samples showing the correct size band for heavy and light chain amplification in the respective wells were subsequently cloned into pAb20-hCHIgG1 (Synbio; for heavy chain) and pAb20-hCK (Synbio; for light chains) through in-fusion cloning (Takara). The ligated product was transformed to DH5-α competent cells ...
-
No products found
because this supplier's products are not listed.
Travis J Gates, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... and underwent antigen retrieval in antigen retrieval buffer (AR9, PerkinElmer) in a 95LC water bath ...
-
No products found
because this supplier's products are not listed.
Jovana Vasiljević, et al.,
bioRxiv - Cell Biology 2021
Quote:
... where anti-mouse light chain specific HRP-conjugated antibodies (Dianova) were used to avoid interference from the heavy chain of the precipitated IgG ...
-
No products found
because this supplier's products are not listed.
Qian Wang, et al.,
bioRxiv - Microbiology 2021
Quote:
... Antibodies were produced by co-transfecting paired heavy- and light-chain antibody-expressing plasmids at a ratio of 1:1 using FectoPRO DNA Transfection Reagent (VWR) into Expi293 cells ...
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No products found
because this supplier's products are not listed.
Xiaoyu Zhao, et al.,
bioRxiv - Immunology 2023
Quote:
... by co-transfection of heavy and light chain expressing plasmids using polyethylenimine (Polyscience) and cells were cultured at 37 °C with shaking at 125 rpm and 8% CO2 ...
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No products found
because this supplier's products are not listed.
Karla Gasparini, et al.,
bioRxiv - Plant Biology 2020
Quote:
... the resin mold was removed from the leaflet surface and filled with nail polish to create a cast that was analyzed by light microscopy (Zeiss Axioskop 40, Carl Zeiss, Jena, Germany). Images were analyzed with Image-Pro ® PLUS ...
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No products found
because this supplier's products are not listed.
Kok-Siong Chen, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Antigen retrieval was performed using an antigen decloaking chamber (Biocare Medical, Pacheco, California). Mounted sections were heated to 125°C for 4 minutes at 15 psi in 10 mM sodium citrate buffer (pH 6.0 ...
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No products found
because this supplier's products are not listed.
Catherine M Sevigny, et al.,
bioRxiv - Cancer Biology 2019
Quote:
SLC7A5 siRNA and plasmid DNA were obtained from OriGene. Products used were SLC7A5 (ID 8140 ...
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Native Antigen
Cat# CMV-CL-100,
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Maya Imbrechts, et al.,
bioRxiv - Immunology 2021
Quote:
The binding of the purified recombinant antibodies to the following SARS-CoV-2 antigens was assessed via ELISA: spike glycoprotein (S1) RBD-His (REC31849-500, The Native Antigen Company), RBD(N439K)-His (40592-V08H14 ...
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No products found
because this supplier's products are not listed.
Anup Kumar Prasad, et al.,
bioRxiv - Biophysics 2023
Quote:
... non-binding surfaces from Greiner Bio-One (Germany ...