-
No products found
because this supplier's products are not listed.
Emma J. Stowe, et al.,
bioRxiv - Bioengineering 2023
Quote:
... was determined via analysis of spent culture medium (n = 5/group/time) using a commercially available FRET-based generic MMP activity kit (SensoLyte 520 Generic MMP Activity Kit Fluorimetric; Anaspec). MMP activity is represented as the concentration of MMP cleaved product (5-FAM-Pro-Leu-OH) ...
-
No products found
because this supplier's products are not listed.
Kelsie J. Anson, Giulia A. Corbet, Amy E. Palmer,
bioRxiv - Cell Biology 2020
Quote:
... we performed an in-vitro plate-based fluorescence assay using recombinant human MKP-3 (Enzo Life Sciences) supplied in 20 mM Tris/HCl ...
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No products found
because this supplier's products are not listed.
Kyle L. Ellefsen, et al.,
bioRxiv - Biophysics 2019
Quote:
FRET-based traction force measurements and Piezo1 Ca2+ flicker measurements were performed with TIRFM on an inverted microscope (Nikon TiE) with an Apo TIRF 100× oil objective lens ...
-
No products found
because this supplier's products are not listed.
Davide De Forni, et al.,
bioRxiv - Microbiology 2021
Quote:
... and an ELISA assay (SARS-CoV-2 Nucleocapsid Detection ELISA Kit, Sino Biological) was performed to measure produced virus through the quantification of the viral NP nucleoprotein (a measure of viral replication capacity).
-
No products found
because this supplier's products are not listed.
Elisabeth Urbauer, et al.,
bioRxiv - Microbiology 2023
Quote:
Total RNA was prepared from isolated colonic epithelial cells of Hsp60Δ/ΔIEC and Hsp60fl/fl mice on day 2 with the column-based NucleoSpin RNAII kit (Macherey-Nagel) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Amalia Sintou, et al.,
bioRxiv - Immunology 2019
Quote:
... Apoptosis assays were performed using Nucleocounter NC-3000 and a Flexicyte apoptosis/necrosis detection kit based on staining with caspase 3 substrate NucView 488 and RedDot 2 (all Biotium, Cambridge Bioscience, UK). Apoptotic cells are defined as NucView488 positive and RedDot2 negative ...
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AdvanStain Scarlet is a fluorescent stain for gels and blots that allows sensitive and...
Cat# K-11072-C25,
25 ml, USD $695.00/ea
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Lopamudra Sadhu, et al.,
bioRxiv - Immunology 2022
Quote:
... Enhanced chemiluminescence (ECL)-based detection using the WesternBright Sirius Chemiluminescent Detection Kit (Advansta) was performed ...
-
No products found
because this supplier's products are not listed.
Gloria Ha, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Cells were regularly validated as mycoplasma free by a PCR-based mycoplasma detection kit (Southern Biotech).
-
No products found
because this supplier's products are not listed.
Huyen Thi Lam Nguyen, et al.,
bioRxiv - Systems Biology 2022
Quote:
... a MACH 3 Rabbit HRP Polymer Detection kit (Biocare Medical # M3R531H) or a MACH 3 Mouse HRP Polymer Detection kit (Biocare Medical # M3M530H ...
-
No products found
because this supplier's products are not listed.
Francesca Petriglieri, et al.,
bioRxiv - Microbiology 2023
Quote:
... DNA was extracted using a custom plate-based extraction protocol based on the FastDNA spin kit for soil (MP Biomedicals). The protocol is available at www.midasfieldguide.org (aau_wwtp_dna_v.8.0) ...
-
No products found
because this supplier's products are not listed.
Jessica Migliavacca, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Addgene)[70] in a ratio of 5:3:2 using polyethylenimine (24765-2, Polysciences). Virus supernatant was harvested 30 h after transfection ...
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No products found
because this supplier's products are not listed.
Taylor L. Hickman, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Intracellular aspartate was quantified using a plate-based Aspartate Colorimetric Assay Kit (BioVision, Cat# K552). Lysates (50 µL ...
-
No products found
because this supplier's products are not listed.
Feng Peng, et al.,
bioRxiv - Microbiology 2023
Quote:
Tn-5 transposon insertion library was build based on EZ-Tn5™ 2>Tnp transposome system (Lucigen, WI, USA). Competent C ...
-
No products found
because this supplier's products are not listed.
Michelle Wantoch, et al.,
bioRxiv - Immunology 2020
Quote:
... 96 well plates were coated with a mixture of antibodies against IFN-α (Mabtech, MT1/3/5) overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Maude Jans, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Fixative was removed by washing 5 x 3 min in 0.1 M cacodylate buffer and samples were incubated in 2% osmium (OsO4, EMS) in 0.1 M cacodylate buffer for 30 min at RT ...
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No products found
because this supplier's products are not listed.
Nioosha Nekooie-Marnany, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Cy-3 or Cy-5 (Jackson Immunoresearch Laboratories), and processed for DAPI or Hoechst staining to visualize cells’ nuclei before mounting in ImmuMount medium (Shandon) ...
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Cat# HY-100564,
inquire
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Lauren C. Farris, et al.,
bioRxiv - Immunology 2023
Quote:
... and STING inhibitor H-151 (HY-112693, MedChemExpress) were added at 10 mM and 0.5 mM ...
-
No products found
because this supplier's products are not listed.
Elizabeth C. Stahl, et al.,
bioRxiv - Bioengineering 2023
Quote:
... The plate-reader based LAL assay was performed with the Endosafe Endochrome-K kit (Charles River, #R1708K), where a control standard endotoxin (CSE ...
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No products found
because this supplier's products are not listed.
Anna Gritsenko, et al.,
bioRxiv - Immunology 2020
Quote:
... 5-Nitro-2-(3-phenylpropylamino)benzoic acid (NPPB, 0593) was sourced from Calbiochem.
-
Single well glass bottom plate with high performance #1.5 cover glass (0.170±0.005mm). Black...
Cat# P01-1.5H,
20/case, $249.00
Ask
Lissenya B. Argueta, et al.,
bioRxiv - Cell Biology 2021
Quote:
... hESC-qualified)-coated plates at 4×10^5/well in 24-well plates or 3×10^4/well in glass-like polymer bottom 96-well plates (CellVis).
-
No products found
because this supplier's products are not listed.
Jianbin Shi, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... A BCA protein detection kit (Solarbio) was used to quantify total protein ...
-
No products found
because this supplier's products are not listed.
Elazar Besser, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... The XTT-based cell proliferation kit (Sartorius, Beit Haemek, Israel) was used to quantitate viability according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Karen Gu, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... This cDNA library was then PCR barcoded with PCR Primer 1.0 (5’-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG-3’) and a sample-specific barcode primer (5’-CAAGCAGAAGACGGCATACGAGATNNNNNNGTGACTGGAGTTCAGACGTG-3’) using MyTaq Red Mix (Bioline) for 15 cycles ...
-
No products found
because this supplier's products are not listed.
Kristina Ulicna, et al.,
bioRxiv - Biophysics 2020
Quote:
... 3 ⨯ 104 cells / cm2 in 24-well plates (ibidi). Imaging was started 2–3 h after seeding ...
-
No products found
because this supplier's products are not listed.
Maciej Jerzy Smialek, et al.,
bioRxiv - Developmental Biology 2019
Quote:
Detection of apoptotic TCam-2 cells was performed 48 h after transfection of TCam-2 cells with control and KIF18A siRNA constructs using an Annexin V-FITC Apoptosis Detection Kit (Beckman Coulter), according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Gaoyang Liang, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... A Luna C18(2) C8 column (3 μm, 2 mm × 50 mm, Phenomenex) was used ...
-
No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... IP of total SUMOylated protein fractions used either SUMO-1 or SUMO-2/3 Ab included with the Signal Seeker SUMOylation 1 or 2/3 Detection Kit (Cytoskeleton) and IPs were carried out according to the manufacturer instructions ...
-
No products found
because this supplier's products are not listed.
Alice Lepelley, et al.,
bioRxiv - Immunology 2021
Quote:
... U2OS-STING cells plated in 12-well plates were transiently transfected using TransIT®-2020 (Mirus) with 500 ng of ATAD3A WT ...
-
No products found
because this supplier's products are not listed.
Ben S. Ou, et al.,
bioRxiv - Bioengineering 2023
Quote:
... aqueous adjuvant (Pam3CSK4, 3M-052, CpG1826, or cGAMP) and RBD-NP were included by subtracting the volume of the antigen and adjuvant cargoes from the volume of TBS 1X ...
-
No products found
because this supplier's products are not listed.
Helen Wegner, et al.,
bioRxiv - Microbiology 2023
Quote:
... in transparent 96-well plates (Sarstedt, PS, flat based). Protein concentrations were determined using a calibration curve with BSA solubilized in the same buffer and measured in a plate reader (Tecan Infinite® F200 Pro ...
-
No products found
because this supplier's products are not listed.
Thi Tuyet Trinh Phan, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... MTT (3-(4,5-dimethylthiazol-2-yl-2, 5-diphenyl tetrazolium bromide)) was purchased from Alfa Aesar (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Jianghua Wu, et al.,
bioRxiv - Immunology 2021
Quote:
... Detection of serum SARS-CoV-2 IgG and IgM antibodies was evaluated by IgM/IgG antibody detection kit (Abbott Laboratories, Inc).
-
No products found
because this supplier's products are not listed.
Yoichi Araki, et al.,
bioRxiv - Neuroscience 2020
Quote:
... or Spinning disk confocal microscopes controlled by axiovision software (Carl Zeiss; Fig. 2, 3, and 5). Following 5-10 min of baseline recording ...
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Prepared to contain higher collagenase and caseinase activities. A dialyzed, lyophilized powder.
Cat# LS005282,
1 gm, $246.00
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Luke R. Perreault, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Minced tissue underwent 3-5 serial digestions in collagenase type 2 (Worthington Biochemical Corp, Lakewood, NJ) in sterile PBS with 20 mM glucose ...
-
No products found
because this supplier's products are not listed.
Lina Streich, et al.,
bioRxiv - Bioengineering 2021
Quote:
... The microscope head and detection unit were based on a Movable Objective Microscope (MOM, Sutter Instrument Company), and three-dimensional translation of the objective was achieved with an electronic micromanipulator translation stage (MP-285) ...
-
No products found
because this supplier's products are not listed.
Eziwoma Alibo, et al.,
bioRxiv - Immunology 2021
Quote:
... Jedi T cells were activated for 3 days with 5 mg/ml plate-bound anti-CD3 mAb (clone 2C11, BioXCell), 1 mg/ml anti-CD28 mAb (clone 37.51 ...
-
No products found
because this supplier's products are not listed.
Sarah O’Keefe, et al.,
bioRxiv - Biochemistry 2021
Quote:
... incubated with appropriate primary antibodies (1:500 or 1:1000 dilution) and processed for fluorescence-based detection as described by LI-COR Biosciences using appropriate secondary antibodies (IRDye 680RD Donkey anti-Goat ...
-
No products found
because this supplier's products are not listed.
Caleb R. Carr, et al.,
bioRxiv - Microbiology 2024
Quote:
... 2 µL of 10 µM 3’ PacBio round 2 reverse primer (PacBio_3pri_RND2), and 25 µL KOD Hot Start Master Mix ...
-
No products found
because this supplier's products are not listed.
Aniruddha Das, et al.,
bioRxiv - Neuroscience 2022
Quote:
... A 3×5 mm2 craniotomy was drilled (Omnidrill35, World Precision Instruments) over an area covering the monocular and binocular primary visual (V1m and V1b ...
-
No products found
because this supplier's products are not listed.
Kamal L Nahas, et al.,
bioRxiv - Cell Biology 2021
Quote:
3 mm gold EM grids with a holey carbon film (R 2/2, 200 mesh; Quantifoil Cat no ...
-
No products found
because this supplier's products are not listed.
Rebecca T. Perelman, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... the 3’-end azide functionalization in presence of 2’-azido-2’-deoxyadenosine-5’triphosphate (ATP-azide, Trilink Biotechnologies) using yeast poly(A ...
-
No products found
because this supplier's products are not listed.
Yan Fang, et al.,
bioRxiv - Genetics 2023
Quote:
800 C3H/10T1/2 cells were seeded in a 96 well plate (glass bottom culture plates, MatTek, PBK96G-1.5-5-F) and keep cells in 37-degree for 24 h ...
-
No products found
because this supplier's products are not listed.
Alessandro Furlan, et al.,
bioRxiv - Neuroscience 2021
Quote:
... coconut oil-based HFD (Envigo; 1 g in 5 ml mineral oil), white chocolate (Lindt ...
-
No products found
because this supplier's products are not listed.
Mary Lou P. Bailey, et al.,
bioRxiv - Cell Biology 2023
Quote:
... cells were imaged on agarose pads (see below) containing 100nM 2-[5-(Adamantan-1-yl)-1H-indol-3-yl]acetic acid (“5-IAA”; TCI Product number A3390).
-
No products found
because this supplier's products are not listed.
Jenny L. M. Digby, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... MEIS1/2/3 (Active Motif 39796) and LTL (Vectorshield FL-1321) ...
-
No products found
because this supplier's products are not listed.
Melody Li, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Patch pipettes of 3-5 MΩ (Harvard Apparatus) were made using a puller (P-1000 ...
-
No products found
because this supplier's products are not listed.
Saskia-L. Jauch-Speer, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 5% CO2 and routinely screened and found negative for mycoplasma contamination in a PCR-based assay (PromoCell).
-
No products found
because this supplier's products are not listed.
Sayantan Roy, Bing Wang, Yuan Tian, Qian Yin,
bioRxiv - Biochemistry 2023
Quote:
... 5 mM MgCl2 and 2 mM Tris (2-carboxyethyl) phosphine (TCEP, GoldBio).
-
No products found
because this supplier's products are not listed.
Shenghui Xing, et al.,
bioRxiv - Microbiology 2021
Quote:
... Chemiluminescent detection was performed using an ECL fluorescence colorimetric kit (Tiangen) and fluorescent signals were visualized using a Bio-Rad Gel Doc XR ...
-
No products found
because this supplier's products are not listed.
J. Christopher Rounds, et al.,
bioRxiv - Neuroscience 2021
Quote:
... sonicated for 3×5 minutes in a 4°C Bioruptor ultrasonicator (UCD-200, Diagenode), vortexed ...